Definition of clinical threshold for CMV real-time PCR after comparison with PP65 antigenaemia and clinical data.

Abstract:

BACKGROUND:pp65 antigenaemia and real-time PCR are two methods that are used to diagnose CMV infection in its early stages and, thereby, to facilitate initiation of pre-emptive therapy. OBJECTIVES:Firstly, to compare PCR with antigenaemia and clinical outcome in order to define a clinical threshold for starting pre-emptive therapy. Secondly, to study the impact of the transplant recipient's serological status on the viral load and on the cut-offs. STUDY DESIGN:Sixty-two patients were analysed using antigenaemia (APAAP method) and real-time PCR. ROC curves were established with antigenaemia or clinical outcome as reference. Patients were divided into primo-infection or reactivation on the basis of the serological status. RESULTS:PCR correlated better with the clinical data (AUC closer to 1 and best sensitivity, PPV and NPV) than antigenaemia. Furthermore, the performance of qPCR was even better in the reactivation patients. CONCLUSIONS:This work suggests that transplant recipients should be divided according to their serological status. Indeed, replacing antigenaemia by real-time PCR for decisions regarding initiation of pre-emptive therapy is of particular appeal in patients with positive serology. As a result of this work, we have set our clinical threshold at 1500 copies/ml for reactivation.

journal_name

Acta Clin Belg

journal_title

Acta clinica Belgica

authors

Mullier F,Kabamba-Mukadi B,Bodéus M,Goubau P

doi

10.1179/acb.2009.083

subject

Has Abstract

pub_date

2009-11-01 00:00:00

pages

477-82

issue

6

eissn

1784-3286

issn

2295-3337

journal_volume

64

pub_type

杂志文章
  • Short-term return visits of 'general internal medicine' patients to the emergency department: extent and risk factors.

    abstract:OBJECTIVES:Although emergency department (ED) return visits are a significant problem universally, it has not been previously studied in our ED. The aim of this study was to determine the extent of the problem in our ED, to identify the relevant clinical predictor variables and to detect diagnostic errors. METHODS:A r...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1179/acb.2009.069

    authors: Vanbrabant P,Knockaert D

    更新日期:2009-09-01 00:00:00

  • [Hypothalamic hypopituitarism with hyperphagia and subacute dementia due to neurosarcoidosis: case report and literature review].

    abstract::The frequency of neurologic involvement in sarcoidosis is about 5%. When the clinical picture presents with only neurological manifestations, as it did in our patient, the diagnosis at presentation is often difficult. Involvement of other tissues must therefore be actively searched. We describe a case of neurosarcoido...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章,评审

    doi:10.1080/17843286.1992.11718250

    authors: Vanhoof J,Wilms G,Bouillon R

    更新日期:1992-01-01 00:00:00

  • Heparin-associated thrombocytopenia: an update.

    abstract::The use of heparin may be complicated by two types of thrombocytopenia (HAT): type I occurs early, is transient, and has no clinical relevance, while type II may lead to very severe manifestations (arterial or venous thromboses and more rarely bleedings), that are still underestimated by some clinicians. HAT-type II m...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章,评审

    doi:10.1080/17843286.1995.11718474

    authors: Fondu P

    更新日期:1995-01-01 00:00:00

  • Liver perfusion and hepatocellular inflammatory response in sepsis.

    abstract::Sepsis is characterized by disturbances in liver perfusion and alterations in intrahepatic cellular functions and interactions. This provokes structural and functional liver damage as well as hepatocellular activation that is believed to perpetuate the immuno-inflammatory response. Changes in hepatic perfusion during ...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章,评审

    doi:

    authors: Smets D,Spapen H,Diltoer M,Nguyen DN,Hubloue I,Huyghens L

    更新日期:1999-08-01 00:00:00

  • Gestational diabetes: overview of the new consensus screening strategy and diagnostic criteria.

    abstract::Gestational diabetes (GDM) is a frequent medical condition during pregnancy. GDM is associated with an increased risk of complications for both the mother and the baby during pregnancy and birth. Women with GDM also have an increased risk of developing type 2 diabetes later in life. Two large randomised intervention t...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章,评审

    doi:10.2143/ACB.67.4.2062669

    authors: Benhalima K,Van Crombrugge P,Hanssens M,Devlieger R,Verhaeghe J,Mathieu C

    更新日期:2012-07-01 00:00:00

  • Subtle relationships between Pseudomonas aeruginosa and fungi in patients with cystic fibrosis.

    abstract::Cystic fibrosis (CF) is one of the most common hereditary lung diseases. Pseudomonas aeruginosa (PA), Aspergillus fumigatus (AF) and Candida albicans (CA) are the principal bacterial and fungal pathogens in the airways of CF patients. The interactions of coexisting bacterial-fungal pathogens are of great interest. In ...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1080/17843286.2020.1852850

    authors: Yan K,Yin H,Wang J,Cai Y

    更新日期:2020-11-26 00:00:00

  • Epidemiology of AKI in the ICU.

    abstract::Up to a little while ago there was no uniform definition for acute kidney injury (AKI). Recently, the Acute Dialysis Quality Initiative proposed the RIFLE consensus classification for AKI. This classification was adapted and modified by the Acute Kidney Injury Network into the AKI staging system. This classification d...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1179/acb.2007.071

    authors: Hoste EA,De Corte W

    更新日期:2007-01-01 00:00:00

  • Isolation of Prototheca zopfii from a finger. Case report and review of the literature.

    abstract::Prototheca zopfii, an alga-like organism, was isolated from subungual material in a patient with onycholysis. This organism seldom causes human infections. Clinical and laboratory aspects are described. The literature is reviewed. ...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章,评审

    doi:10.1080/17843286.1991.11718171

    authors: Magerman K,Gordts B,Hindryckx P,Van Landuyt HW

    更新日期:1991-01-01 00:00:00

  • Donor cell leukemia developing after hematopoietic stem cell transplantation for multiple myeloma.

    abstract::The development of secondary leukemia in donor cells after allogeneic stem cell transplantation is a rare event. We describe the occurrence of acute myeloid leukemia in donor cells 4 years after a stem cell transplantation for multiple myeloma. The multiple myeloma was relapsing at the time of the onset of acute myelo...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1179/acb.2006.016

    authors: Havelange V,Antoine-Poirel H,Saussoy P,Van Den Neste E,Ferrant A

    更新日期:2006-03-01 00:00:00

  • Febrile neutropenia induces changes in insulin sensitivity similar to obesity.

    abstract::Background/aim: To determine insulin sensitivity before chemotherapy and during febrile neutropenia in patients with acute leukemia and to assess its effect on the number of documented infections, the severity of infection and the outcome of the first hospitalization. To compare insulin sensitivity in the study group ...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1080/17843286.2018.1534577

    authors: Perčić I,Urošević I,Stokić E,Tomić Naglić D,Milošević I

    更新日期:2019-12-01 00:00:00

  • Simultaneous determination of seven azole antifungal drugs in serum by ultra-high pressure liquid chromatography and diode array detection.

    abstract::Azole antifungals are a group of fungistatic agents that can be administered orally or parenterally. The determination of the concentrations of these antifungals (miconazole, fluconazole, ketoconazole, posaconazole, voriconazole, itraconazole, and its major active metabolite, hydroxy-itraconazole) in serum can be usef...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1179/0001551213Z.00000000018

    authors: Mistretta V,Dubois N,Denooz R,Charlier C

    更新日期:2014-01-01 00:00:00

  • Repair for partial and complete atrioventricular septal defect: single centre experience and long-term results.

    abstract:BACKGROUND:The number of adult patients with surgical repaired congenital heart defects increases continuously. We were interested to compare late outcome after partial and complete atrioventricular defect repair (pAVSD and cAVSD, respectively) and to determine the most important reason for re-intervention. METHODS:Al...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:

    authors: Soufflet V,Daenen W,Van Deyk K,Troost E,Budts W

    更新日期:2005-09-01 00:00:00

  • Postprandial reactive hypoglycaemia in a very old patient.

    abstract:OBJECTIVE AND IMPORTANCE:Postprandial reactive hypoglycaemia (PRH) is a clinical syndrome characterized by the recurrence of symptomatic hypoglycaemia during postprandial periods. PRH remains a diagnostic challenge for clinicians, because of its atypical manifestations and low prevalence, especially in older persons. ...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1179/2295333714Y.0000000026

    authors: Deliens C,Losseau C,Vandeleene B,Boland B

    更新日期:2014-08-01 00:00:00

  • Long-term oxygen therapy with concentrators and liquid oxygen.

    abstract::The National Institute for Insurance of Disease and Disablement (RIZIV/INAMI) in Belgium reimburses the costs of long term oxygen therapy (LTOT) by concentrators since 1984 and by liquid oxygen since 1988 for specific categories of patients supervised by approved medical centers. Since then we prescribed these therapi...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1080/17843286.1995.11718439

    authors: Buyse B,Demedts M

    更新日期:1995-01-01 00:00:00

  • Intra-abdominal pressure measurements in lateral decubitus versus supine position.

    abstract:OBJECTIVE:Intra-abdominal pressure (IAP) has traditionally been measured in the supine position, however, measuring the pressure in lateral semi-recumbent position has not been studied. DESIGN:A single centre prospective 1-day study. PATIENTS:10 patients admitted for more then 24 hours who were mechanically ventilate...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1179/acb.2009.037

    authors: De Keulenaer BL,Cheatham ML,De Waele JJ,Kimball EJ,Powell B,Davis WA,Jenkins IR

    更新日期:2009-05-01 00:00:00

  • A rare presentation of kidney failure in a patient with giant cell arteritis: case report and review of literature.

    abstract::Although giant cell arteritis, also called temporal arteritis, is the most common primary vasculitis in the elderly, an association with AA amyloidosis has rarely been reported. AA amyloidosis is a disorder that results from the extracellular deposition of proteolytic cleavage products of serum amyloid A, which occurs...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1080/17843286.2020.1763671

    authors: Van De Ginste L,Dendooven A,Van Dorpe J,Delanghe JR,Vanmassenhove J,Speeckaert MM

    更新日期:2020-05-12 00:00:00

  • [Wegener's granulomatosis: clinical, radiological and diagnostic characteristics. Apropos of a case].

    abstract::The authors report an observation of Wegener's granulomatosis with pulmonary, bronchial, renal, cutaneous and sinusal involvement. Five years ago, the patient was referred to us because of bilateral diffuse pulmonary infiltrates of unknown origin. A corticotherapy was introduced at that time and a complete clearance o...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章,评审

    doi:10.1080/17843286.1995.11718464

    authors: Polus M,Hamels J,Dorzee J,Henkinbrant A,Carlier B,d'Odemont JP

    更新日期:1995-01-01 00:00:00

  • Primary sclerosing cholangitis and idiopathic pulmonary fibrosis: a case report.

    abstract::Primary sclerosing cholangitis (PSC) and idiopathic pulmonary fibrosis (IPF) were each separately described in association with a variety of chronic idiopathic inflammatory diseases such as retroperitoneal fibrosis, Sicca complex or Riedel's thyroiditis. We report a case which may be the first description of the assoc...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1080/17843286.1989.11717982

    authors: Jonard P,Geubel A,Wallon J,Rahier J,Dive C,Meunier H

    更新日期:1989-01-01 00:00:00

  • The kidney in the critically ill.

    abstract::Acute kidney injury (AKI) is a common and serious complication in the intensive care setting. It seldom occurs in isolation, but is mostly part of a multiple organ dysfunction syndrome. The pathogenesis is frequently multifactorial, with sepsis contributing to 50% of the cases.The development of AKI in critically-ill ...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章,评审

    doi:10.1179/acb.2007.033

    authors: Schetz M

    更新日期:2007-07-01 00:00:00

  • Pulmonary Langerhans' cell granulomatosis (histiocytosis X): clinical analysis of 8 cases.

    abstract::A detailed retrospective analysis of 8 cases has been set up to obtain an update of the clinical, radiological, diagnostic and therapeutical aspects of pulmonary Langerhans' cell granulomatosis (PLCG), previously called Histiocytosis X. This disease represents 2.8% of interstitial lung diseases (ILD) in a registration...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1080/17843286.1998.11754186

    authors: Callebaut W,Demedts M,Verleden G

    更新日期:1998-10-01 00:00:00

  • Relapsing infiltrates after pneumocystis pneumonia in stem cell transplant patients: think about BOOP!

    abstract::Bronchiolitis Obliterans Organizing Pneumonia (BOOP) can complicate allogeneic haematopoietic stem cell transplantation. It is associated with prior graft-versus-host disease (GVHD) and the case fatality is 21%. In 22%, diagnosis is preceded by tapering the corticosteroids given as a treatment for GVHD. We report a fa...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1179/acb.2010.042

    authors: Deeren D,Lammertijn L,Van Dorpe J

    更新日期:2010-05-01 00:00:00

  • Taenia saginata a rare cause of acute cholangitis: a case report.

    abstract::Parasitic infestations of the galdbladder and biliary tract are quite rare. Taenia saginata is an intestinal helmint and patients harbouring adult T.saginata tapeworms are mostly asymptomatic and discharge only fecal proglottids. In some cases there might be nonspecific symptoms like vomiting, nausea, epigastric pain,...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.2143/ACB.67.6.2062709

    authors: Uygur-Bayramiçli O,Ak O,Dabak R,Demirhan G,Ozer S

    更新日期:2012-11-01 00:00:00

  • [Unexpected origin of recurrent ascites: apropos of 2 cases].

    abstract::The authors report the cases of two patients suffering from ascites attributed for several years to a non pericarditic aetiology. The first patient presented with a diagnosis of right cardiac failure secondary to a right myocardial infarction. Cardiac catheterisation, magnetic resonance imaging and transoesophageal ec...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1080/17843286.1997.11718570

    authors: Heureux F,Frankart L,Marchandise B,Buche M,Martinet JP,Donckier J

    更新日期:1997-01-01 00:00:00

  • A woman with recurrent "infections" since birth--a new mevalonate kinase mutation.

    abstract::A tired 32-year-old woman complaining of tiredness was referred for work-up of a possible immune deficiency. She had a history of recurrent infections since birth, which usually responded to antibiotics within a few days. Her mother, a nurse, had reported that early charts had disappeared. Munchausen's by proxy was su...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.2143/ACB.66.2.2062531

    authors: Farber CM,Wanders JA,Goffard JC,Parma J

    更新日期:2011-03-01 00:00:00

  • In vitro and in vivo evaluation of two factor VIII concentrates virally inactivated by solvent-detergent or by pasteurization.

    abstract::The present study compares two new factor VIII preparations currently used in the treatment of haemophilia. A Factor VIII was partially purified from plasma obtained from unpaid voluntary Belgian donors in the blood transfusion centers of Lille and Amsterdam and virus-inactivated by exposure to solvent-detergent (FVII...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1080/17843286.1991.11718180

    authors: Peerlinck K,Arnout J,Tamise M,Vanherle A,Fondu P,Vermylen J

    更新日期:1991-01-01 00:00:00

  • Streptococcus pyogenes bacteremia: a retrospective study in a Belgian university hospital.

    abstract::We retrospectively studied 29 cases of S.pyogenes bacteremia, treated from September 1985 through September 1993, in order to define the clinical pattern, predisposing factors and the outcome of S.pyogenes bacteremia in our region. The number of infections, the clinical picture and mortality rate of S.pyogenes bactere...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1080/17843286.1995.11718427

    authors: Van de Mierop F,Knockaert DC,De Raedt S,Verhaegen J,Peetermans WE

    更新日期:1995-01-01 00:00:00

  • Massive pleural and pericardial effusion due to hypothyroidism in a patient with a surgically treated thyroid-stimulating hormone-producing pituitary adenoma.

    abstract::Hypothyroidism is relatively rare etiology of serositis with effusion, but massive pleural effusion is very unusual. This is a report of massive pleural effusion in patient taking methimazole after surgical resection of thyroid-stimulating hormone (TSH)-producing pituitary adenoma (TSHoma). The patient was clinically ...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1080/17843286.2018.1474619

    authors: Lee JH,Park M,Park MJ,Jo YS

    更新日期:2018-10-01 00:00:00

  • Recovery of pulmonary and skin lesions of sarcoidosis after thymectomy.

    abstract:OBJECTIVE AND IMPORTANCE:Sarcoidosis is a multisystem inflammatory disorder of unknown etiology. It is characterized by the presence of noncaseating granulomas and an inflammatory process in which T lymphocytes, especially type-1 helper T (Th1) cells, macrophages and different cytokines are involved. Different studies ...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1080/17843286.2016.1152671

    authors: Esendagli D,Koksal D,Emri S

    更新日期:2016-12-01 00:00:00

  • Is systematic screening and treatment for latent tuberculosis infection in HIV patients useful in a low endemic setting?

    abstract:OBJECTIVES:A decreasing incidence of tuberculosis (TB) among HIV patients has been documented in high-income settings and screening for tuberculosis is not systematically performed in many clinics (such as ours). Our objectives are to evaluate whether a same decline of incidence was seen in our Belgian tertiary center ...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章

    doi:10.1080/17843286.2016.1237696

    authors: Maniewski U,Payen MC,Delforge M,De Wit S

    更新日期:2017-08-01 00:00:00

  • Diagnosis and treatment of hypertensive disorders during pregnancy.

    abstract::Pregnancy is a cardiovascular and metabolic challenge to the human female body. This review summarizes current knowledge on the regulation of blood pressure and plasma volume in normal and hypertensive pregnant women. During pregnancy, systemic vascular resistance and blood pressure decrease, whereas cardiac output an...

    journal_title:Acta clinica Belgica

    pub_type: 杂志文章,评审

    doi:10.1179/acb.2010.050

    authors: Fabry IG,Richart T,Chengz X,Van Bortel LM,Staessen JA

    更新日期:2010-07-01 00:00:00