Abstract:
OBJECTIVES:This study was designed to identify the characteristics and surface antigen properties of mesenchymal stromal cells (MSC) isolated and cultured from bone marrow of healthy subjects and to assess their differentiation potential to osteoblast and chondroblast lineage cells. METHODS:Mononuclear cells were isolated by density-gradient separation from 1-3 ml of bone marrow collected from 10 donors of bone transplantation aged between 4 months and 18 years. These mononuclear cells were cultured in flasks containing 10% fetal calf serum, which resulted in growth of fibroblast-like cells showing adhesion onto the culture flask. Physical properties of the cells were identified and flow cytometric immunophenotyping was performed to specify surface antigen properties. Differentiation of mesenchymal stromal cells in specific media to chondroblasts and osteoblasts was evaluated. Osteoblasts and chondroblasts were stained with Alizarin red and Alcian blue, respectively, and microphotographed. RESULTS:In vitro yield of MSCs showed no age-related differences in terms of morphological and adhesive properties. While cells of stromal origin showed strong positivity (90% to 99%) to characteristic CD105, CD44, CD166, CD29, CD90, and CD73 antibodies, hematopoietic cells remained negative (0% to 5%) to CD45, CD34, CD14, and HLA-DR antibodies. It was observed that MSCs produced in cell-specific media differentiated to osteoblasts and chondroblasts in all passages (p1-15) tested, including late passages. CONCLUSION:It seems that the use of MSCs would provide promising treatment strategies in bone marrow transplantation, inherited diseases, and organ repair; in in vitro assessment of biological effects of biomaterials in orthopedics; and in repair of bone and cartilage injuries.
journal_name
Acta Orthop Traumatol Turcjournal_title
Acta orthopaedica et traumatologica turcicaauthors
Kiliç E,Ceyhan T,Cetinkaya DUsubject
Has Abstractpub_date
2007-08-01 00:00:00pages
295-301issue
4eissn
1017-995Xissn
2589-1294journal_volume
41pub_type
杂志文章abstract::Tuberculosis is one of the oldest diseases affecting mankind and is known for its ability to present in various forms and guises. Pubic symphysis is an uncommon site for tuberculous affliction; hence very few cases have been reported in the English-language literature. We present a rare case of pubic symphysis tubercu...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/aott.2012.2696
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVES:We retrospectively evaluated patients who underwent arthroscopic treatment for superior labrum anterior posterior (SLAP) lesions combined with antero-inferior labral detachment (Bankart lesion, Maffet type 5). METHODS:The study included eight male patients (mean age 31.8 years; range 23 to 50 years) who wer...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:OBJECTIVE:This study aimed to evaluate whether a history of a pelvic osteotomy or proximal femoral osteotomy compromises the outcomes of total hip arthroplasty in patients with dysplastic coxarthrosis. METHODS:The results of total hip arthroplasty in 240 hips of 172 patients without previous pelvic osteotomy or proxim...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.5152/j.aott.2020.01.7
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:Proximal and diaphyseal humeral fractures are common especially in the elderly, presenting as a challenging problem due to their high complication rates following surgical treatment. In this prospective study, we evaluated the results of patients treated with the PHILOS (Proximal Humeral Internal Locking Sys...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/aott.2008.42.2.097
更新日期:2008-03-01 00:00:00
abstract::Closed reduction attempts may be unsuccessful after traumatic knee dislocations on rare occasions. The interposition of the soft tissues on the medial aspect of the joint into the femoral condyle and tibial plateau is shown to be the cause of an unsuccessful reduction. In such cases, open reduction is the recommended ...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2011.2377
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVE:The present study aimed to compare the isometric strength and endurance of shoulder abduction and internal and external rotation between operated shoulders and nonoperated, contralateral shoulders of patients who underwent reverse shoulder replacement due to unilateral rotator cuff tear arthropathy. PATIENTS...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.1016/j.aott.2019.08.001
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to examine the changes in the lumbar intervertebral area after extracorporeal shockwave therapy (ESWT) at different energy levels in a rabbit model using magnetic resonance imaging (MRI) and histopathological evaluation. METHODS:The study included 30 male New Zealand white rabbits d...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/aott.2012.2931
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVES:Patients whose anterior cruciate ligament (ACL) was completely ruptured described a feeling as if they had lost their lower extremity below the knee and gave an account that they had not sensed that part of their extremity at the time of ACL rupture. Thinking that this description might denote a diagnostic s...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Osteochondritis dissecans (OCD) is a condition in which a portion of articular cartilage separates from the bone together with the underlying subchondral bone. Its classical localization is the medial femoral condyle. We presented a 14-year-old boy who had OCD lesions in both the medial femoral condyle and patella in ...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2009.072
更新日期:2009-01-01 00:00:00
abstract:OBJECTIVES:This study was designed to determine normal values of pedobarography during standing and walking in adolescents in our country and to investigate correlations between demographic data and pedobarographic values. METHODS:Fifty volunteers (25 girls, 25 boys; mean age 14 years; range 13 to 15 years) who were f...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract:OBJECTIVES:The results of open reduction and internal fixation of humeral shaft fractures by either an intramedullary nail or a dynamic compression plate were compared. METHODS:The study included 60 patients (43 males, 17 females; mean age 38 years; range 19 to 61 years) with humerus fractures. Thirty-three patients w...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:OBJECTIVES:We evaluated the functional results of open reduction and internal fixation with a locking plate in patients with three- or four-part fractures of the proximal humerus. METHODS:We reviewed 32 consecutive patients (22 women, 10 men; mean age 63 years; range 29 to 82 years) who were treated with open reductio...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2010.2275
更新日期:2010-01-01 00:00:00
abstract::Fibroma of the tendon sheath (FTS) is a rare benign tumour that usually develops in the upper extremity, particularly in the fingers, hands and wrists. Herein, we present the case of a patient with an unusually localised FTS compressing the superficial branch of the radial nerve. A 62-year-old woman presented with a s...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.1016/j.aott.2019.04.007
更新日期:2019-09-01 00:00:00
abstract::This article presents a 48-year-old male patient who presented with pain in the left forearm and weakness and clumsiness in the left hand of 6 months' duration. Flexor motor strength loss of the thumb and the index finger was present and neurophysiologic tests showed findings compatible with axonal injury in the anter...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.1016/j.aott.2017.09.011
更新日期:2017-12-01 00:00:00
abstract::Pelvic digit was incidentally detected on plain pelvis radiography of a 44-year-old male patient who presented with right-sided hip pain. Three-dimensional reconstruction of computed tomography scans provided a more clear illustration of the anatomy and localization of this anomaly. To avoid unnecessary investigation ...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:OBJECTIVES:We evaluated the contribution of subacromial local anesthetic and corticosteroid injection to the conservative treatment of subacromial impingement syndrome. METHODS:The study included 56 patients (39 women, 17 men; mean age 50 years; range 31 to 68 years) with subacromial impingement syndrome without any r...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章,随机对照试验
doi:10.3944/AOTT.2009.331
更新日期:2009-08-01 00:00:00
abstract:OBJECTIVES:We evaluated the results of primary open reduction and internal fixation with the Herbert-Whipple screw in acute displaced carpal scaphoid fractures. METHODS:The study included 10 patients with acute displaced carpal scaphoid fractures and one patient with transscaphoid perilunate fracture dislocation. All ...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to determine whether the Taylor spatial frame (TSF) can precisely correct deformities around the knee and whether application of TSF is easy and safe for treatment of the deformities around the knee. METHODS:This study included 50 retrospectively reviewed limbs of 37 patients (mean ...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/aott.2013.2958
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:The visual analog scale spine score (VASSS) is a valid and reliable instrument for outcome assessment of patients with thoracic and lumbar spine fractures. The aim of this study was to prepare a Turkish version of the VASSS and to validate its use for assessing treatment outcomes in Turkish patients with spin...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2011.2528
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to examine the use of magnetically controlled growing rods as a method of providing spinal improvement while preventing thoracic insufficiency in patients with early-onset scoliosis (EOS). METHODS:Of a total of 13 patients, 4 patients underwent a dual magnetic rod implantation, whil...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.1016/j.aott.2017.12.004
更新日期:2018-11-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to describe the application of the rapid prototyping (RP) life-size 3-dimensional model used to improve accuracy of screw insertion in irreducible atlanto-axial dislocation (IAD). METHODS:The study included 10 patients with IAD. All patients were assessed using the Japanese Orthoped...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2014.13.0005
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to compare the clinical and radiographic outcomes of clavicular hook plate fixation with and without coracoclavicular (CC) tape augmentation for the treatment of acute unstable AC dislocation. METHODS:We treated 47 patients (31 men and 16 women; mean age: 47 years (range, 21-81)) wi...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.1016/j.aott.2019.08.002
更新日期:2019-11-01 00:00:00
abstract::In this case report a 38-year-old woman who presented with swelling and pain on her right ankle for two years is presented. Physical and radiological examinations revealed a soft, lobulated and fixed mass in the ankle. During surgical resection shiny, whitish bodies were removed from the tendon sheath of tibialis ante...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/aott.2013.2683
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:Two different rat models for degloving injury were described in the literature. Our aim in this study is to compare these rat models to determine which one is more reliable and reproducible. METHODS:We surgically induced degloving injury on tails and left hindlimbs of Wistar albino rats (n = 8), and sutured ...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.1016/j.aott.2017.03.007
更新日期:2017-07-01 00:00:00
abstract::Ganglion cysts represent a rare pathology mostly encountered in the lumbar region of the spinal column. Magnetic resonance imaging revealed a ganglion cyst at the L4-5 level in a 46-year-old woman who had a complaint of long-standing pain in her right leg. The cyst was completely excised following total laminectomy at...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract:OBJECTIVES:Thirty-nine percent of the ankle and midfoot fractures in ankle distortions could be missed during initial evaluation in emergency department because of inadequate clinical and radiological evaluation in a limited time. We aimed to evaluate the follow-up and treatment outcomes of subjects with missed fractur...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2010.2328
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study is to evaluate the functional results of arthroscopic lateral epicondylitis (LE) treatment in patients in whom conservative treatment proved insufficient. METHODS:Between 2011-2014, 29 patients with LE (15 women, 14 men; mean age: 46 years; range: 33-79) who received at least 6 months o...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2015.15.0048
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to compare tunnel expansion and isokinetic muscle strength after single- and dual-bundle reconstruction of the anterior cruciate ligament (ACL). METHODS:This study included 34 patients who underwent ACL reconstruction in our clinic between November 2007 and March 2008. Eighteen pati...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章,随机对照试验
doi:10.3944/aott.2012.2749
更新日期:2012-01-01 00:00:00
abstract::Dislocation and fracture-dislocation of the knee are serious injuries, often related to high-energy trauma. Irreducibility with closed techniques is an extremely rare and challenging problem that has been described in posterolateral, posterior and lateral knee dislocations. Irreducibility in fracture-dislocations arou...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2014.3112
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVES:We investigated the effect of extracorporeal shock wave therapy in the treatment of lateral epicondylitis. METHODS:The study included 13 elbows of 12 patients (8 women, 4 men; mean age 40 years) who did not benefit from conservative therapy for lateral epicondylitis and refused surgical therapy. Under fluor...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00