Abstract:
OBJECTIVES:This study was designed to identify the characteristics and surface antigen properties of mesenchymal stromal cells (MSC) isolated and cultured from bone marrow of healthy subjects and to assess their differentiation potential to osteoblast and chondroblast lineage cells. METHODS:Mononuclear cells were isolated by density-gradient separation from 1-3 ml of bone marrow collected from 10 donors of bone transplantation aged between 4 months and 18 years. These mononuclear cells were cultured in flasks containing 10% fetal calf serum, which resulted in growth of fibroblast-like cells showing adhesion onto the culture flask. Physical properties of the cells were identified and flow cytometric immunophenotyping was performed to specify surface antigen properties. Differentiation of mesenchymal stromal cells in specific media to chondroblasts and osteoblasts was evaluated. Osteoblasts and chondroblasts were stained with Alizarin red and Alcian blue, respectively, and microphotographed. RESULTS:In vitro yield of MSCs showed no age-related differences in terms of morphological and adhesive properties. While cells of stromal origin showed strong positivity (90% to 99%) to characteristic CD105, CD44, CD166, CD29, CD90, and CD73 antibodies, hematopoietic cells remained negative (0% to 5%) to CD45, CD34, CD14, and HLA-DR antibodies. It was observed that MSCs produced in cell-specific media differentiated to osteoblasts and chondroblasts in all passages (p1-15) tested, including late passages. CONCLUSION:It seems that the use of MSCs would provide promising treatment strategies in bone marrow transplantation, inherited diseases, and organ repair; in in vitro assessment of biological effects of biomaterials in orthopedics; and in repair of bone and cartilage injuries.
journal_name
Acta Orthop Traumatol Turcjournal_title
Acta orthopaedica et traumatologica turcicaauthors
Kiliç E,Ceyhan T,Cetinkaya DUsubject
Has Abstractpub_date
2007-08-01 00:00:00pages
295-301issue
4eissn
1017-995Xissn
2589-1294journal_volume
41pub_type
杂志文章abstract:OBJECTIVES:The aim of this study was to evaluate Turkish trends in zone 2 flexor repair with regards to surgical technique, suture materials, anesthesia and post-operative rehabilitation and compare this with international surgeons by modifying Gibson's survey. METHODS:A printed and online survey consisting of 19 ques...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章,多中心研究
doi:10.1016/j.aott.2019.07.003
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate changes in lesser trochanter shapes in relation to femoral rotations and to develop a reference value for the determination of clinically relevant malrotation of the femur. METHODS:Patients who underwent computed tomography angiography between January 2009 and July 2018 ...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.5152/j.aott.2020.02.126
更新日期:2020-03-01 00:00:00
abstract::We report a case of a pseudoaneurysm of the medial circumflex femoral artery that presented 4 months following cementless left total hip arthroplasty (THA). A successful embolization was achieved using super-selective catheterization and coil embolization. Arterial complications associated with THA are remarkably rare...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2014.3000
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:Metallic implants in the first metatarsophalangeal (MTP) joint have been used for many years in the treatment of hallux rigidus (HR). The HemiCAP(®) prosthesis is the first implant used for resurfacing the metatarsal head in HR treatment. The aim of our study was to evaluate the early results of the HemiCAP(®...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/aott.2012.2610
更新日期:2012-01-01 00:00:00
abstract::We present a 21-year-old male patient with an isolated ulnopalmar dislocation of the fifth carpometacarpal joint that occurred due to a fall on the hand. Diagnosis was delayed for five weeks and closed reduction was not successful. He was treated with open reduction and internal fixation. Upon removal of fixation mate...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract:OBJECTIVE:The purpose of this study was to analyze and demonstrate the heterogeneity in adult spinal deformity (ASD) populations by baseline health-related quality of life (HRQL) data. METHODS:ASD was defined as patients over 18 years of age with any of the following: coronal deformity >20°, sagittal vertical axis (SV...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2016.14.0421
更新日期:2016-01-01 00:00:00
abstract::The number of primary hip replacement procedures performed each year has exponentially increased, with a subsequent increase in associated revision procedures. Prosthetic infection is a serious concern for all arthroplasty surgeons. Psoas abscess is a rare condition with unknown etiology, and diagnosis is often missed...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2014.2994
更新日期:2014-01-01 00:00:00
abstract::Cartilage injuries can be treated through conservative or surgical approaches upon evaluation of the lesion. In surgical approach, arthroscopic treatment has proven to be both very popular and efficient. Through arthroscopy, treatment options such as lavage, chondral shaving, debridement, microfracture, abrasion, and ...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章,评审
doi:
更新日期:2007-01-01 00:00:00
abstract:OBJECTIVE:The aim of this anatomical study was to compare the effects of the prone and lateral decubitus positions in endoscopic disc surgery on the Kambin's triangle (KT) and neural foramina zones in the lumbosacral region. METHODS:The study included 32 healthy volunteers (16 females and 16 males). Bilateral KT areas...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2015.14.0233
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the effectiveness of cryotherapy applied for the purpose of matricectomy following partial nail removal in treatment of ingrown nails. METHODS:Between 2010 and 2013, 76 ingrown toenails of 62 patients were included in this prospective study. Following partial toenail ...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2015.15.0117
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVES:Proximal and diaphyseal humeral fractures are common especially in the elderly, presenting as a challenging problem due to their high complication rates following surgical treatment. In this prospective study, we evaluated the results of patients treated with the PHILOS (Proximal Humeral Internal Locking Sys...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/aott.2008.42.2.097
更新日期:2008-03-01 00:00:00
abstract:OBJECTIVE:The prognostic factors that affect sensory nerve recovery after digital nerve repair are variable because of nonhomogeneous data, subjective tests, and different assessment/scoring methods. The aim of this study was to evaluate the success of sensory nerve recovery after digital nerve repair and to investigat...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2015.15.0140
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVE:This study is an evaluation of the overall survival rate and factors affecting survival in patients with osteosarcoma, chondrosarcoma, or Ewing's sarcoma. This study aimed to determine the effect of factors related to the preoperative period, patient, tumor, treatment, and postoperative period on survival. M...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.5152/j.aott.2020.03.79
更新日期:2020-05-01 00:00:00
abstract:OBJECTIVES:We evaluated the midterm results of total knee arthroplasty in degenerative knee joint diseases with severe deformity. METHODS:Total knee arthroplasty was performed in 125 knees of 86 patients (75 women, 11 men; mean age 69 years; range 39 to 85 years) with degenerative knee joint diseases accompanied by se...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/aott.2008.001
更新日期:2008-01-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to determine 1) the publication rates of podium and poster presentations from the 23rd (2013) and the 24th (2014) National Turkish Orthopedics and Traumatology Congresses in peer-reviewed journals and (2) compare these rates with publication rates from the 20th congress (2007) publi...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.1016/j.aott.2019.05.006
更新日期:2019-07-09 00:00:00
abstract:OBJECTIVES:We evaluated the results of external rotation osteotomy of the humerus for the treatment of shoulder problems secondary to obstetric brachial plexus palsy. METHODS:Forty children (24 boys, 16 girls; mean age 7.5 years; range 23 months to 14.8 years) underwent external rotation osteotomy of the humerus. Invo...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract:OBJECTIVE:This study aimed to evaluate whether a history of a pelvic osteotomy or proximal femoral osteotomy compromises the outcomes of total hip arthroplasty in patients with dysplastic coxarthrosis. METHODS:The results of total hip arthroplasty in 240 hips of 172 patients without previous pelvic osteotomy or proxim...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.5152/j.aott.2020.01.7
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to define histopathological features of giant cell tumor of bone, especially accompanying fibrohistiocytic or aneurysmal bone cyst like components, in the light of our institutions experience. METHODS:A total of 120 cases (64 females and 56 males; mean age: 36.2 (12-80)) with 'GCT' ...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.1016/j.aott.2018.10.007
更新日期:2019-01-01 00:00:00
abstract:PURPOSE:This study investigated the short-term effects of KT on back pain (BP) in patients with Lenke Type 1 adolescent idiopathic scoliosis (AIS). METHODS:We chosen Lenke Type 1 scoliosis who have had only back pain (the localization of the pain: the only in the apical convex edge). Forty patients suffering from BP w...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.aott.2017.01.002
更新日期:2017-05-01 00:00:00
abstract:OBJECTIVE:Our aim was to evaluate the results of minimally invasive plate osteosynthesis (MIPO) using locking plates in diaphyseal humerus and proximal humerus fractures. METHODS:Nine patients who underwent open reduction and MIPO for the treatment of diaphyseal and proximal humerus fractures between June 2006 and Oct...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/aott.2012.2592
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to compare tunnel expansion and isokinetic muscle strength after single- and dual-bundle reconstruction of the anterior cruciate ligament (ACL). METHODS:This study included 34 patients who underwent ACL reconstruction in our clinic between November 2007 and March 2008. Eighteen pati...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章,随机对照试验
doi:10.3944/aott.2012.2749
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to compare the long-term results of two groups of patients with trochanteric fractures of the femur treated with either dynamic hip screw (DHS) or AO angled blade plate (AP) insertions. METHODS:A total of 157 patients with trochanteric fractures were included in the study, and 82 p...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章,随机对照试验
doi:10.3944/AOTT.2010.2356
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVES:We compared the electrophysiologic and histopathologic results of grafting and distraction methods in the treatment of defective peripheral neural injuries. METHODS:Thirty male white rabbits (age 6 months) were divided into three groups and a circular external fixator (CEF) was applied to the right tibias. ...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to compare the clinical and radiographic outcomes of clavicular hook plate fixation with and without coracoclavicular (CC) tape augmentation for the treatment of acute unstable AC dislocation. METHODS:We treated 47 patients (31 men and 16 women; mean age: 47 years (range, 21-81)) wi...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.1016/j.aott.2019.08.002
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to analyze and compare the preoperative anamnestic details between patients with an arthroscopic diagnosis of bucket handle and other tear patterns of the medial meniscus in stable knees. METHODS:A total of 204 patients (mean age 49.3 ± 13 years) were included in the study. The mean...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.1016/j.aott.2016.03.009
更新日期:2016-12-01 00:00:00
abstract::One of the standard treatment methods for developmental dysplasia of the hip is closed reduction followed by immobilization in a hip spica cast. This treatment is performed before achievement of independent walking, under general anesthesia and with arthrographic control. Avoidance of forced reductions and immobilizat...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章,评审
doi:
更新日期:2007-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to compare the sleep quality of patients who underwent total knee arthroplasty before and after the surgery and analyze the effect of total knee arthroplasty on sleep quality. METHODS:The study included 42 patients (32 females, 10 males) who underwent total knee arthroplasty for pri...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2014.3163
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVES:This study was designed to evaluate anatomical and functional results of non-surgical treatment for unstable distal radius fractures in the elderly. METHODS:Twenty-nine patients (7 males, 22 females; mean age 72+/-2 years) aged = or > 65 years were treated with closed reduction and short-arm circular castin...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2009.229
更新日期:2009-05-01 00:00:00
abstract:OBJECTIVE:To describe a clinical treatment algorithm for posterior ankle impingement (PAI) syndrome in professional football players. MATERIAL AND METHODS:A case series of 26 elite professional football players diagnosed and treated for posterior ankle impingement syndrome were included for the study. All of the athle...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.1016/j.aott.2016.03.008
更新日期:2016-12-01 00:00:00
abstract::Dislocation and fracture-dislocation of the knee are serious injuries, often related to high-energy trauma. Irreducibility with closed techniques is an extremely rare and challenging problem that has been described in posterolateral, posterior and lateral knee dislocations. Irreducibility in fracture-dislocations arou...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2014.3112
更新日期:2014-01-01 00:00:00