Abstract:
OBJECTIVES:This study was designed to identify the characteristics and surface antigen properties of mesenchymal stromal cells (MSC) isolated and cultured from bone marrow of healthy subjects and to assess their differentiation potential to osteoblast and chondroblast lineage cells. METHODS:Mononuclear cells were isolated by density-gradient separation from 1-3 ml of bone marrow collected from 10 donors of bone transplantation aged between 4 months and 18 years. These mononuclear cells were cultured in flasks containing 10% fetal calf serum, which resulted in growth of fibroblast-like cells showing adhesion onto the culture flask. Physical properties of the cells were identified and flow cytometric immunophenotyping was performed to specify surface antigen properties. Differentiation of mesenchymal stromal cells in specific media to chondroblasts and osteoblasts was evaluated. Osteoblasts and chondroblasts were stained with Alizarin red and Alcian blue, respectively, and microphotographed. RESULTS:In vitro yield of MSCs showed no age-related differences in terms of morphological and adhesive properties. While cells of stromal origin showed strong positivity (90% to 99%) to characteristic CD105, CD44, CD166, CD29, CD90, and CD73 antibodies, hematopoietic cells remained negative (0% to 5%) to CD45, CD34, CD14, and HLA-DR antibodies. It was observed that MSCs produced in cell-specific media differentiated to osteoblasts and chondroblasts in all passages (p1-15) tested, including late passages. CONCLUSION:It seems that the use of MSCs would provide promising treatment strategies in bone marrow transplantation, inherited diseases, and organ repair; in in vitro assessment of biological effects of biomaterials in orthopedics; and in repair of bone and cartilage injuries.
journal_name
Acta Orthop Traumatol Turcjournal_title
Acta orthopaedica et traumatologica turcicaauthors
Kiliç E,Ceyhan T,Cetinkaya DUsubject
Has Abstractpub_date
2007-08-01 00:00:00pages
295-301issue
4eissn
1017-995Xissn
2589-1294journal_volume
41pub_type
杂志文章abstract:OBJECTIVE:This study aimed to investigate the academic productivity of and the obstacles encountered by orthopedic residents in Turkey. METHODS:Overall, 220 orthopedic specialists who were registered in the Ministry of Health and had started orthopedic residency between 2009 and 2010 were invited to participate in a s...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.5152/j.aott.2020.03.243
更新日期:2020-05-01 00:00:00
abstract:OBJECTIVES:The results of open reduction and internal fixation of humeral shaft fractures by either an intramedullary nail or a dynamic compression plate were compared. METHODS:The study included 60 patients (43 males, 17 females; mean age 38 years; range 19 to 61 years) with humerus fractures. Thirty-three patients w...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:OBJECTIVE:Delaying the treatment of bone and tendon injuries may cause unmanageable complications. Bonesetters continue to cause delays in treatment. The purpose of this study was to analyze the medical outcomes of delay due to bonesetter intervention and factors affecting patient treatment preference. METHODS:Among o...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2015.14.0358
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the mid-term results of patients with surgically treated 4th and 5th carpometacarpal (CMC) fracture dislocation. METHODS:The study included 9 CMC dislocation patients (9 males; mean age: 31.2 years, range: 20 to 40 years) treated with open reduction and internal fixation...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2014.14.0040
更新日期:2014-01-01 00:00:00
abstract::Nontyphoidal salmonella (NTS) infection can lead to gastroenteritis, enteric fever, and bacteremia. However, osteoarticular infections caused by NTS are rarely encountered. We report the case of a 53-year-old male patient with bilateral hip infection caused by NTS. We performed a two-stage reconstruction comprising de...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type:
doi:10.5152/j.aott.2020.02.278
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVE:The visual analog scale spine score (VASSS) is a valid and reliable instrument for outcome assessment of patients with thoracic and lumbar spine fractures. The aim of this study was to prepare a Turkish version of the VASSS and to validate its use for assessing treatment outcomes in Turkish patients with spin...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2011.2528
更新日期:2011-01-01 00:00:00
abstract::A unique case of large intra-articular osteochondroma of the knee of a 15-year duration is presented along with a review of the literature. The tumor may have remained asymptomatic for such a long period because of its slow growth and stretch elongation of the quadriceps mechanism. ...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章,评审
doi:10.3944/AOTT.2012.2542
更新日期:2012-01-01 00:00:00
abstract::We present a 51-year-old male patient with Brucella abortus septic arthritis in the right knee following arthroscopic meniscus surgery. He had eaten a traditional dish of raw minced cattle conceptus (bovine fetus) that was prepared after the cow was slaughtered. Despite treatment with empirical antibiotics and debride...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2015.14.0287
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study is to evaluate the functional results of arthroscopic lateral epicondylitis (LE) treatment in patients in whom conservative treatment proved insufficient. METHODS:Between 2011-2014, 29 patients with LE (15 women, 14 men; mean age: 46 years; range: 33-79) who received at least 6 months o...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2015.15.0048
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:Metallic implants in the first metatarsophalangeal (MTP) joint have been used for many years in the treatment of hallux rigidus (HR). The HemiCAP(®) prosthesis is the first implant used for resurfacing the metatarsal head in HR treatment. The aim of our study was to evaluate the early results of the HemiCAP(®...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/aott.2012.2610
更新日期:2012-01-01 00:00:00
abstract::Partial meniscectomy is the most widely used intervention in the arthroscopic treatment of meniscal disorders. Despite improved arthroscopic techniques and instrumentation, surgery of the lateral compartment is still challenging. The "wheel track" technique, first described here, will facilitate partial meniscectomy i...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the short-term outcomes of metatarsal head metal resurfacing hemiarthroplasty in patients with advanced stage hallux rigidus. METHODS:The study included 14 feet (4 left, 10 right) of 12 patients (10 female, 2 male; mean age: 63 ± 5; range: 55 to 71 years) who underwent m...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/aott.2012.2795
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVES:We evaluated the functional results of open reduction and internal fixation with a locking plate in patients with three- or four-part fractures of the proximal humerus. METHODS:We reviewed 32 consecutive patients (22 women, 10 men; mean age 63 years; range 29 to 82 years) who were treated with open reductio...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2010.2275
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVES:Giant-cell tumors of the tendon sheath localized in the hand or wrist were retrospectively reviewed with respect to diagnosis, surgical treatment, and postoperative complications. METHODS:During a 21-year period, a total of 141 patients (83 females, 58 males; mean age 37.5 years; range 6 to 77 years) underw...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to compare the long-term results of two groups of patients with trochanteric fractures of the femur treated with either dynamic hip screw (DHS) or AO angled blade plate (AP) insertions. METHODS:A total of 157 patients with trochanteric fractures were included in the study, and 82 p...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章,随机对照试验
doi:10.3944/AOTT.2010.2356
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to compare tunnel expansion and isokinetic muscle strength after single- and dual-bundle reconstruction of the anterior cruciate ligament (ACL). METHODS:This study included 34 patients who underwent ACL reconstruction in our clinic between November 2007 and March 2008. Eighteen pati...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章,随机对照试验
doi:10.3944/aott.2012.2749
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the outcomes of open reduction and internal fixation (ORIF) in hamate hook fractures and review the literature on this surgical procedure. METHODS:We report the outcomes of ORIF of hamate hook fractures in 13 consecutive patients (12 men and 1 woman; mean age: 32 years (...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.1016/j.aott.2018.12.005
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVES:We evaluated the results of external rotation osteotomy of the humerus for the treatment of shoulder problems secondary to obstetric brachial plexus palsy. METHODS:Forty children (24 boys, 16 girls; mean age 7.5 years; range 23 months to 14.8 years) underwent external rotation osteotomy of the humerus. Invo...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to compare the Winograd and knot techniques based on efficiency, complication rate, surgery time, and amount of local anesthetic required. This study also aimed to determine the etiology of ingrown nails, whether due to involvement of the nail or soft tissue. METHODS:Seventy-five pa...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2015.14.0450
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVES:We evaluated the results of the Ilizarov method in the treatment of pseudoarthrosis of the lower extremities. METHODS:Forty-six patients (34 men, 12 women; mean age 38.6 years; range 28 to 69 years) were treated by the Ilizarov method for femoral (n=8, 17%) and tibial (n=38, 83%) pseudoarthrosis. The mean d...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the results of multifunctional intramedullary (IM) ulna nailing following diaphyseal fracture of the ulna. METHODS:Adult patients with isolated fractures of the ulna treated with closed or mini-open reduction using the new IM ulna nail between May 2008 and January 2011 a...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/aott.2013.3021
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:Two different rat models for degloving injury were described in the literature. Our aim in this study is to compare these rat models to determine which one is more reliable and reproducible. METHODS:We surgically induced degloving injury on tails and left hindlimbs of Wistar albino rats (n = 8), and sutured ...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.1016/j.aott.2017.03.007
更新日期:2017-07-01 00:00:00
abstract:OBJECTIVE:Intramedullary nailing is the preferred surgical treatment of humerus shaft fractures. The purpose of this study was to investigate the relationship between the bicipital groove and specific anatomical landmarks in achieving correct alignment of the humerus during intramedullary nailing, and to describe these...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2015.14.0188
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to evaluate Turkish trends in zone 2 flexor repair with regards to surgical technique, suture materials, anesthesia and post-operative rehabilitation and compare this with international surgeons by modifying Gibson's survey. METHODS:A printed and online survey consisting of 19 ques...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章,多中心研究
doi:10.1016/j.aott.2019.07.003
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to compare the efficiency of dorsal tangential fluoroscopy and ultrasonography in detecting dorsal screw penetration in distal radius volar locking plate applications. METHODS:Ten cadaveric forearms were operated. The distal four screws were protruded 0, 1 and 2 mm into each of the ...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.1016/j.aott.2017.10.004
更新日期:2017-12-01 00:00:00
abstract::Triceps tendon rupture is a rare condition. A thirty-three year old man presented with a complaint of pain in his left elbow, which occurred when his motorcycle slid and flipped on one side. Physical examination two days after the accident showed swelling and ecchymosis in the elbow. There was a palpable, slightly ten...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:OBJECTIVE:To investigate the relationship between injury patterns, complications, and the functional outcomes of patients with proximal-ulna fracture-dislocations. METHODS:Retrospective analysis of 15 patients (10 men, 5 women; mean age, 49.1 years; mean follow-up 49 months) with 6 anterior and 9 posterior fracture-di...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2015.14.0178
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:This study aimed to determine the relationship between red cell distribution width (RDW) and mortality in patients that received a partial hip prosthesis. METHODS:The study included 316 patients (183 female and 133 male) that underwent surgery due to hip fracture and were followed up for ≥1 year. Mean age of...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:10.3944/AOTT.2014.2859
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVES:We evaluated the efficacy and short-term results of medial opening wedge high tibial osteotomy with the use of a Puddu plate in patients with medial compartment gonarthrosis. METHODS:The study included 15 knees of 12 female patients (mean age 50.6 years; range 45 to 63 years) who were treated with medial op...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:OBJECTIVES:We evaluated the results of lengthening of the phalanges by callus distraction in traumatic amputations of the fingers. METHODS:We treated traumatic amputations of 16 fingers of 13 male patients (mean age 27.7 years; range 12 to 43 years) by callotasis of the phalanges. Callus distraction was performed with...
journal_title:Acta orthopaedica et traumatologica turcica
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00