Abstract:
:Nuclear receptors play important roles in the maintenance of the endocrine system, regulation of organ differentiation, and fetal development. Endocrine disruptors exert their adverse effects by disrupting the endocrine system via various mechanisms. To assess the effects of endocrine disruptors on nuclear receptors, we developed a high-throughput method for identifying activators of nuclear receptors. Using this system, we found that triphenyltin and tributyltin were activators of peroxisome proliferator-activated receptor (PPAR) gamma and retinoid X receptor. Because PPARgamma is a master regulator of adipocyte differentiation, we assessed the effect of organotin compounds on preadipocyte 3T3-L1 cells. We found that organotin compounds stimulated differentiation of 3T3-L1 cells as well as expression of adipocyte marker genes.
journal_name
Mol Pharmacoljournal_title
Molecular pharmacologyauthors
Kanayama T,Kobayashi N,Mamiya S,Nakanishi T,Nishikawa Jdoi
10.1124/mol.104.008409keywords:
subject
Has Abstractpub_date
2005-03-01 00:00:00pages
766-74issue
3eissn
0026-895Xissn
1521-0111pii
mol.104.008409journal_volume
67pub_type
杂志文章abstract::The mechanisms underlying mastoparan-induced elevation of the intracellular free calcium concentration ([Ca2+]i) were investigated in the insulin-secreting cell lines RINm5F and HIT. In both cell types, micromolar concentrations of mastoparan induced a prompt increase of [Ca2+]i, measured as an increase in fura-2 fluo...
journal_title:Molecular pharmacology
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更新日期:2005-04-01 00:00:00
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journal_title:Molecular pharmacology
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journal_title:Molecular pharmacology
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journal_title:Molecular pharmacology
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journal_title:Molecular pharmacology
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doi:
更新日期:1996-12-01 00:00:00