Deinococcus indicus sp. nov., an arsenic-resistant bacterium from an aquifer in West Bengal, India.

Abstract:

:An arsenic- and radiation-resistant bacterium, strain Wt/1a(T), was isolated from water from an arsenic-contaminated aquifer located in the Chakdah district of West Bengal, India. The bacterium stains Gram-negative and is rod-shaped, non-motile, non-sporulating and red-pigmented. Cell-wall peptidoglycan contains ornithine as the diamino acid, MK-8 is the major menaquinone, C(15 : 1) and C(16 : 1) are the major fatty acids and the DNA G+C content of the organism is 65.8 mol%. Based on these phenotypic and chemotaxonomic characteristics, strain Wt/1a(T) was identified as a member of the genus Deinococcus. Strain Wt/1a(T) exhibited maximum 16S rRNA gene sequence similarity (95 %) with Deinococcus grandis; however, strain Wt/1a(T) exhibited only 14 % similarity to D. grandis IAM 13005(T) at the DNA-DNA level. Furthermore, strain Wt/1a(T) (compared to D. grandis IAM 13005(T)) is more resistant to arsenate and arsenite, is positive for arginine dihydrolase, utilizes a number of carbon sources and exhibits quantitative differences in fatty acid composition and qualitative differences in lipid composition. Strain Wt/1a(T) is identified as a novel species of the genus Deinococcus, for which the name Deinococcus indicus sp. nov. is proposed. The type strain of Deinococcus indicus is Wt/1a(T) (=MTCC 4913(T)=DSM 15307(T)).

authors

Suresh K,Reddy GSN,Sengupta S,Shivaji S

doi

10.1099/ijs.0.02758-0

keywords:

subject

Has Abstract

pub_date

2004-03-01 00:00:00

pages

457-461

issue

Pt 2

eissn

1466-5026

issn

1466-5034

journal_volume

54

pub_type

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