Noncoding plastid trnT-trnF sequences reveal a well resolved phylogeny of basal angiosperms.

Abstract:

:Recent contributions from DNA sequences have revolutionized our concept of systematic relationships in angiosperms. However, parts of the angiosperm tree remain unclear. Previous studies have been based on coding or rDNA regions of relatively conserved genes. A phylogeny for basal angiosperms based on noncoding, fast-evolving sequences of the chloroplast genome region trnT-trnF is presented. The recognition of simple direct repeats allowed a robust alignment. Mutational hot spots appear to be confined to certain sectors, as in two stem-loop regions of the trnL intron secondary structure. Our highly resolved and well-supported phylogeny depicts the New Caledonian Amborella as the sister to all other angiosperms, followed by Nymphaeaceae and an Austrobaileya-Illicium-Schisandra clade. Ceratophyllum is substantiated as a close relative of monocots, as is a monophyletic eumagnoliid clade consisting of Piperales plus Winterales sister to Laurales plus Magnoliales. Possible reasons for the striking congruence between the trnT-trnF based phylogeny and phylogenies generated from combined multi-gene, multi-genome data are discussed.

journal_name

J Evol Biol

authors

Borsch T,Hilu KW,Quandt D,Wilde V,Neinhuis C,Barthlott W

doi

10.1046/j.1420-9101.2003.00577.x

keywords:

subject

Has Abstract

pub_date

2003-07-01 00:00:00

pages

558-76

issue

4

eissn

1010-061X

issn

1420-9101

journal_volume

16

pub_type

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