Green fluorescent protein as a visual marker in somatic hybridization.

Abstract:

:Using a transgenic citrus plant expressing Green Fluorescent Protein (GFP) as a parent in somatic fusion experiments, we investigated the suitability of GFP as an in vivo marker to follow the processes of protoplast fusion, regeneration and selection of hybrid plants. A high level of GFP expression was detected in transgenic citrus protoplasts, hybrid callus, embryos and plants. It is demonstrated that GFP can be used for the continuous monitoring of the fusion process, localization of hybrid colonies and callus, and selection of somatic hybrid embryos and plants.

journal_name

Ann Bot

journal_title

Annals of botany

authors

Olivares-Fuster O,Peña L,Duran-Vila N,Navarro L

doi

10.1093/aob/mcf054

keywords:

subject

Has Abstract

pub_date

2002-04-01 00:00:00

pages

491-7

issue

4

eissn

0305-7364

issn

1095-8290

journal_volume

89

pub_type

杂志文章
  • SF21 is a Protein which Exhibits a Dual Nuclear and Cytoplasmic Localization in Developing Pistils of Sunflower and Tobacco.

    abstract::SF21 was originally described as a pollen- and pistil-expressed protein from sunflower and tobacco. In pistils excised from these species, transcripts were detected in the stigma and in the transmitting tissue where they accumulated in an ovary-oriented increasing concentration gradient. We studied the cellular locali...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1006/anbo.2000.1319

    authors: Kräuter-Canham R,Bronner R,Steinmetz A

    更新日期:2001-02-01 00:00:00

  • Phylogenetic relationships among arecoid palms (Arecaceae: Arecoideae).

    abstract:BACKGROUND AND AIMS:The Arecoideae is the largest and most diverse of the five subfamilies of palms (Arecaceae/Palmae), containing >50 % of the species in the family. Despite its importance, phylogenetic relationships among Arecoideae are poorly understood. Here the most densely sampled phylogenetic analysis of Arecoid...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcr020

    authors: Baker WJ,Norup MV,Clarkson JJ,Couvreur TL,Dowe JL,Lewis CE,Pintaud JC,Savolainen V,Wilmot T,Chase MW

    更新日期:2011-12-01 00:00:00

  • Exogenous ethylene inhibits sprout growth in onion bulbs.

    abstract:BACKGROUND AND AIMS:Exogenous ethylene has recently gained commercial interest as a sprouting inhibitor of onion bulbs. The role of ethylene in dormancy and sprouting of onions, however, is not known. METHODS:A cultivar (Allium cepa 'Copra') with a true period of dormancy was used. Dormant and sprouting states of onio...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcn203

    authors: Bufler G

    更新日期:2009-01-01 00:00:00

  • The barley MATE gene, HvAACT1, increases citrate efflux and Al(3+) tolerance when expressed in wheat and barley.

    abstract:BACKGROUND AND AIMS:Aluminium is toxic in acid soils because the soluble Al(3+) inhibits root growth. A mechanism of Al(3+) tolerance discovered in many plant species involves the release of organic anions from root apices. The Al(3+)-activated release of citrate from the root apices of Al(3+)-tolerant genotypes of bar...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mct135

    authors: Zhou G,Delhaize E,Zhou M,Ryan PR

    更新日期:2013-08-01 00:00:00

  • Biomechanical properties of marsh vegetation in space and time: effects of salinity, inundation and seasonality.

    abstract:BACKGROUND AND AIMS:Over the last decade, the importance of plant biomechanical properties in shaping wave dissipation efficiency of marsh vegetation has gained growing attention. Here we provide the first analyses of how biomechanical stem properties vary with seasons and along environmental gradients in coastal and e...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcz063

    authors: Zhu Z,Yang Z,Bouma TJ

    更新日期:2020-02-03 00:00:00

  • Assessment of enzyme induction and aerenchyma formation as mechanisms for flooding tolerance in Trifolium subterraneum 'Park'.

    abstract::The objective of this study was to evaluate the role of enzyme induction and aerenchyma formation in prolonged tolerance to soil flooding in a variety of underground clover (Trifolium subterraneum 'Park') previously selected for resistance. Seedlings were grown in hydroponic tanks, initially with aeration for 3 weeks ...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcf022

    authors: Aschi-Smiti S,Chaibi W,Brouquisse R,Ricard B,Saglio P

    更新日期:2003-01-01 00:00:00

  • 'Hemiepiphyte': a confusing term and its history.

    abstract:BACKGROUND AND SCOPE:Over more than 120 years of scientific study since Schimper's seminal work, the recognized categories of structurally dependent plants have changed several times. Currently, ignoring parasitic mistletoes, it is usual to distinguish four functional groups: (1) true epiphytes; (2) primary hemiepiphyt...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mct085

    authors: Zotz G

    更新日期:2013-06-01 00:00:00

  • How can we take advantage of halophyte properties to cope with heavy metal toxicity in salt-affected areas?

    abstract:BACKGROUND:Many areas throughout the world are simultaneously contaminated by high concentrations of soluble salts and by high concentrations of heavy metals that constitute a serious threat to human health. The use of plants to extract or stabilize pollutants is an interesting alternative to classical expensive decont...

    journal_title:Annals of botany

    pub_type: 杂志文章,评审

    doi:10.1093/aob/mcu264

    authors: Lutts S,Lefèvre I

    更新日期:2015-02-01 00:00:00

  • Variable response of three Trifolium repens ecotypes to soil flooding by seawater.

    abstract:BACKGROUND AND AIMS:Despite concerns about the impact of rising sea levels and storm surge events on coastal ecosystems, there is remarkably little information on the response of terrestrial coastal plant species to seawater inundation. The aim of this study was to elucidate responses of a glycophyte (white clover, Tri...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcu118

    authors: White AC,Colmer TD,Cawthray GR,Hanley ME

    更新日期:2014-08-01 00:00:00

  • Orchid conservation: making the links.

    abstract::Orchidaceae, one of the largest families of flowering plants, present particular challenges for conservation, due in great part to their often complex interactions with mycorrhizal fungi, pollinators and host trees. In this Highlight, we present seven papers focusing on orchids and their interactions and other factors...

    journal_title:Annals of botany

    pub_type:

    doi:10.1093/aob/mcv142

    authors: Fay MF,Pailler T,Dixon KW

    更新日期:2015-09-01 00:00:00

  • Recovery dynamics of growth, photosynthesis and carbohydrate accumulation after de-submergence: a comparison between two wetland plants showing escape and quiescence strategies.

    abstract:BACKGROUND AND AIMS:The capacity for fast-growth recovery after de-submergence is important for establishment of riparian species in a water-level-fluctuation zone. Recovery patterns of two wetland plants, Alternanthera philoxeroides and Hemarthria altissima, showing 'escape' and 'quiescence' responses, respectively, d...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcq212

    authors: Luo FL,Nagel KA,Scharr H,Zeng B,Schurr U,Matsubara S

    更新日期:2011-01-01 00:00:00

  • Predicted changes in vegetation structure affect the susceptibility to invasion of bryophyte-dominated subarctic heath.

    abstract:BACKGROUND AND AIMS:A meta-analysis of global change experiments in arctic tundra sites suggests that plant productivity and the cover of shrubs, grasses and dead plant material (i.e. litter) will increase and the cover of bryophytes will decrease in response to higher air temperatures. However, little is known about w...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcr097

    authors: Eckstein RL,Pereira E,Milbau A,Graae BJ

    更新日期:2011-07-01 00:00:00

  • Genome size variation at constant chromosome number is not correlated with repetitive DNA dynamism in Anacyclus (Asteraceae).

    abstract:BACKGROUND AND AIMS:Changes in the amount of repetitive DNA (dispersed and tandem repeats) are considered the main contributors to genome size variation across plant species in the absence of polyploidy. However, the study of repeatome dynamism in groups showing contrasting genomic features and complex evolutionary his...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcz183

    authors: Vitales D,Álvarez I,Garcia S,Hidalgo O,Nieto Feliner G,Pellicer J,Vallès J,Garnatje T

    更新日期:2020-03-29 00:00:00

  • Pushed to the limit: consequences of climate change for the Araucariaceae: a relictual rain forest family.

    abstract:BACKGROUND AND AIMS:Under predicted climate change scenarios, increased temperatures are likely to predispose trees to leaf and other tissue damage, resulting in plant death and contraction of already narrow distribution ranges in many relictual species. The effects of predicted upward temperatures may be further exace...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcr135

    authors: Offord CA

    更新日期:2011-08-01 00:00:00

  • Effects of self-, chase and mixed self/cross-pollinations on pistil longevity and fruit set in Ceiba species (Bombacaceae) with late-acting self-incompatibility.

    abstract:BACKGROUND AND AIMS:Late-acting self-incompatibility (LSI), in which selfed flowers fail to form fruits despite apparently successful growth of the pollen tubes to the ovules, is a contentious and still poorly understood phenomenon. Some studies have indicated pollen tube-pistil interactions, and major gene control. Ot...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mch141

    authors: Gibbs P,Bianchi MB,Taroda Ranga N

    更新日期:2004-08-01 00:00:00

  • Retracing the contours of the early angiosperm environmental niche.

    abstract:BACKGROUND AND AIMS:Our aim was to understand the environmental conditions of the emergence and radiation of early angiosperms. Such a question has long remained controversial because various approaches applied in the past have drawn conflicting images of early angiosperm ecology. METHODS:We provided a new perspective...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcz131

    authors: Pouteau R,Trueba S,Isnard S

    更新日期:2020-01-08 00:00:00

  • Generalist passerine pollination of a winter-flowering fruit tree in central China.

    abstract:BACKGROUND AND AIMS:Winter-flowering plants outside the tropics may experience a shortage of pollinator service, given that insect activity is largely limited by low temperature. Birds can be alternative pollinators for these plants, but experimental evidence for the pollination role of birds in winter-flowering plants...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcr293

    authors: Fang Q,Chen YZ,Huang SQ

    更新日期:2012-02-01 00:00:00

  • Field patterns of leaf plasticity in adults of the long-lived evergreen Quercus coccifera.

    abstract:BACKGROUND AND AIMS:Quercus coccifera, as a long-lived sprouter, responds plastically to environmental variation. In this study, the role of foliar plasticity as a mechanism of habitat selection and modification within the canopy and across contrasted habitats was characterized. An examination was made of the different...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcm112

    authors: Rubio De Casas R,Vargas P,Pérez-Corona E,Manrique E,Quintana JR,García-Verdugo C,Balaguer L

    更新日期:2007-08-01 00:00:00

  • Divergent evolution of the chloroplast small heat shock protein gene in the genera Rhododendron (Ericaceae) and Machilus (Lauraceae).

    abstract:BACKGROUND AND AIMS:Evolutionary and ecological roles of the chloroplast small heat shock protein (CPsHSP) have been emphasized based on variations in protein contents; however, DNA sequence variations related to the evolutionary and ecological roles of this gene have not been investigated. In the present study, a basa...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcl288

    authors: Wu ML,Lin TP,Lin MY,Cheng YP,Hwang SY

    更新日期:2007-03-01 00:00:00

  • Ontogeny of embryogenic callus in Medicago truncatula: the fate of the pluripotent and totipotent stem cells.

    abstract:BACKGROUND AND AIMS:Understanding the fate and dynamics of cells during callus formation is essential to understanding totipotency and the mechanisms of somatic embryogenesis. Here, the fate of leaf explant cells during the development of embryogenic callus was investigated in the model legume Medicago truncatula. MET...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcq269

    authors: Wang XD,Nolan KE,Irwanto RR,Sheahan MB,Rose RJ

    更新日期:2011-04-01 00:00:00

  • AmapSim: a structural whole-plant simulator based on botanical knowledge and designed to host external functional models.

    abstract:BACKGROUND AND AIMS:AmapSim is a tool that implements a structural plant growth model based on a botanical theory and simulates plant morphogenesis to produce accurate, complex and detailed plant architectures. This software is the result of more than a decade of research and development devoted to plant architecture. ...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcm194

    authors: Barczi JF,Rey H,Caraglio Y,de Reffye P,Barthélémy D,Dong QX,Fourcaud T

    更新日期:2008-05-01 00:00:00

  • A genetic resource for early-morning flowering trait of wild rice Oryza officinalis to mitigate high temperature-induced spikelet sterility at anthesis.

    abstract:BACKGROUND AND AIMS:High temperatures over 32-36 degrees C at anthesis induce spikelet sterility in rice. The use of a germplasm with an early-morning flowering (EMF) trait has been hypothesized as a way of avoiding this problem. In this study, the effect of the EMF trait on avoiding high temperature-induced sterility ...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcq124

    authors: Ishimaru T,Hirabayashi H,Ida M,Takai T,San-Oh YA,Yoshinaga S,Ando I,Ogawa T,Kondo M

    更新日期:2010-09-01 00:00:00

  • Nutrient availability and atmospheric CO2 partial pressure modulate the effects of nutrient heterogeneity on the size structure of populations in grassland species.

    abstract:BACKGROUND AND AIMS:Size-asymmetric competition occurs when larger plants have a disproportionate advantage in competition with smaller plants. It has been hypothesized that nutrient heterogeneity may promote it. Experiments testing this hypothesis are inconclusive, and in most cases have evaluated the effects of nutri...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcl093

    authors: Maestre FT,Reynolds JF

    更新日期:2006-07-01 00:00:00

  • Physiological changes in gentian axillary buds during two-step preculturing with sucrose that conferred high levels of tolerance to desiccation and cryopreservation.

    abstract:BACKGROUND AND AIMS:Induction of dehydration tolerance is a key to achieving high survival rates in cryopreservation of plant specimens. It has been reported previously that two-step preculturing with sucrose effectively increased desiccation tolerance in axillary buds of gentian (Gentiana scabra), which allow the buds...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcl054

    authors: Suzuki M,Ishikawa M,Okuda H,Noda K,Kishimoto T,Nakamura T,Ogiwara I,Shimura I,Akihama T

    更新日期:2006-06-01 00:00:00

  • Elucidating the interaction between light competition and herbivore feeding patterns using functional-structural plant modelling.

    abstract:Background and Aims:Plants usually compete with neighbouring plants for resources such as light as well as defend themselves against herbivorous insects. This requires investment of limiting resources, resulting in optimal resource distribution patterns and trade-offs between growth- and defence-related traits. A plant...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcx212

    authors: de Vries J,Poelman EH,Anten N,Evers JB

    更新日期:2018-04-18 00:00:00

  • Biomechanical responses of aquatic plants to aerial conditions.

    abstract:BACKGROUND AND AIMS:Wetlands are impacted by changes in hydrological regimes that can lead to periods of low water levels. During these periods, aquatic plants experience a drastic change in the mechanical conditions that they encounter, from low gravitational and tensile hydrodynamic forces when exposed to flow under ...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mct221

    authors: Hamann E,Puijalon S

    更新日期:2013-12-01 00:00:00

  • Authenticated DNA from ancient wood remains.

    abstract:BACKGROUND:The reconstruction of biological processes and human activities during the last glacial cycle relies mainly on data from biological remains. Highly abundant tissues, such as wood, are candidates for a genetic analysis of past populations. While well-authenticated DNA has now been recovered from various fossi...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcl188

    authors: Liepelt S,Sperisen C,Deguilloux MF,Petit RJ,Kissling R,Spencer M,de Beaulieu JL,Taberlet P,Gielly L,Ziegenhagen B

    更新日期:2006-11-01 00:00:00

  • Temporal dynamics in wheat grain zinc distribution: is sink limitation the key?

    abstract:BACKGROUND AND AIMS:Enhancing the zinc (Zn) concentration in wheat (Triticum aestivum) grain is a breeding objective in order to improve human Zn nutrition. At enhanced plant Zn uptake, grain Zn levels do not increase proportionally and within the grain the endosperm Zn levels remain below grain Zn levels. This study a...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcr040

    authors: Stomph TJ,Choi EY,Stangoulis JC

    更新日期:2011-05-01 00:00:00

  • Physiological, biochemical and transcriptional analysis of onion bulbs during storage.

    abstract:BACKGROUND AND AIMS:During the transition from endo-dormancy to eco-dormancy and subsequent growth, the onion bulb undergoes the transition from sink organ to source, to sustain cell division in the meristematic tissue. The mechanisms controlling these processes are not fully understood. Here, a detailed analysis of wh...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcr318

    authors: Chope GA,Cools K,Hammond JP,Thompson AJ,Terry LA

    更新日期:2012-03-01 00:00:00

  • Contrasting hydraulic architecture and function in deep and shallow roots of tree species from a semi-arid habitat.

    abstract:BACKGROUND AND AIMS:Despite the importance of vessels in angiosperm roots for plant water transport, there is little research on the microanatomy of woody plant roots. Vessels in roots can be interconnected networks or nearly solitary, with few vessel-vessel connections. Species with few connections are common in arid ...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mct294

    authors: Johnson DM,Brodersen CR,Reed M,Domec JC,Jackson RB

    更新日期:2014-03-01 00:00:00