Abstract:
:Using a transgenic citrus plant expressing Green Fluorescent Protein (GFP) as a parent in somatic fusion experiments, we investigated the suitability of GFP as an in vivo marker to follow the processes of protoplast fusion, regeneration and selection of hybrid plants. A high level of GFP expression was detected in transgenic citrus protoplasts, hybrid callus, embryos and plants. It is demonstrated that GFP can be used for the continuous monitoring of the fusion process, localization of hybrid colonies and callus, and selection of somatic hybrid embryos and plants.
journal_name
Ann Botjournal_title
Annals of botanyauthors
Olivares-Fuster O,Peña L,Duran-Vila N,Navarro Ldoi
10.1093/aob/mcf054keywords:
subject
Has Abstractpub_date
2002-04-01 00:00:00pages
491-7issue
4eissn
0305-7364issn
1095-8290journal_volume
89pub_type
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