Abstract:
:Protein transduction domains (PTD), which can transport proteins or peptides across biological membranes, have been identified in several proteins of viral, invertebrate, and vertebrate origin. Here, we evaluate the immunological and biological consequences of including PTD in synthetic peptides and in DNA vaccines that contain CD8(+) T cell epitopes from lymphocytic choriomeningitis virus (LCMV). Synthetic PTD-peptides did not induce detectable CD8(+) T cell responses. However, fusion of an open reading frame encoding a PTD to an epitope minigene caused transfected tissue culture cells to stimulate epitope-specific T cells much more effectively. Kinetic studies indicated that the epitope reached the surface of transfected cells more rapidly and that the number of transfected cells needed to stimulate T cell responses was reduced by 35- to 50-fold when compared to cells transfected with a standard minigene plasmid. The mechanism underlying the effect of PTD linkage is not clear, but transit of the PTD-attached epitope from transfected cells to nontransfected cells (cross presentation) seemed to play, at most, a minimal role. Mice immunized once with the plasmid encoding the PTD-linked epitope showed a markedly accelerated CD8(+) T cell response and, unlike mice immunized with a standard plasmid, were completely protected against a normally lethal LCMV challenge administered only 8 days post-immunization.
journal_name
Hum Gene Therjournal_title
Human gene therapyauthors
Leifert JA,Lindencrona JA,Charo J,Whitton JLdoi
10.1089/104303401753153938keywords:
subject
Has Abstractpub_date
2001-10-10 00:00:00pages
1881-92issue
15eissn
1043-0342issn
1557-7422journal_volume
12pub_type
杂志文章abstract::Newcastle disease virus (NDV) is a naturally oncolytic virus that has been shown to be safe and effective for cancer therapy. Tumor virotherapy using NDV emerged in the 1950s and has advanced more recently by the increased availability of reverse genetics technology. In this study, we constructed a reverse genetics sy...
journal_title:Human gene therapy
pub_type: 杂志文章
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journal_title:Human gene therapy
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doi:10.1089/hum.1997.8.14-1675
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journal_title:Human gene therapy
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更新日期:2013-10-01 00:00:00
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journal_title:Human gene therapy
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journal_title:Human gene therapy
pub_type: 杂志文章
doi:10.1089/hum.1996.7.13-1491
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journal_title:Human gene therapy
pub_type: 临床试验,杂志文章
doi:10.1089/hum.1998.9.16-2407
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journal_title:Human gene therapy
pub_type: 杂志文章
doi:10.1089/hum.1992.3.1-11
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journal_title:Human gene therapy
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journal_title:Human gene therapy
pub_type: 杂志文章
doi:10.1089/hum.2012.012
更新日期:2012-11-01 00:00:00
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journal_title:Human gene therapy
pub_type: 杂志文章
doi:10.1089/hum.2017.220
更新日期:2018-08-01 00:00:00
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journal_title:Human gene therapy
pub_type: 临床试验,杂志文章
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更新日期:2005-08-01 00:00:00
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journal_title:Human gene therapy
pub_type: 杂志文章
doi:10.1089/hum.1996.7.2-197
更新日期:1996-01-20 00:00:00
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journal_title:Human gene therapy
pub_type: 杂志文章
doi:10.1089/hum.2016.154
更新日期:2016-11-01 00:00:00
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journal_title:Human gene therapy
pub_type: 杂志文章
doi:10.1089/hum.2005.16.893
更新日期:2005-07-01 00:00:00
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journal_title:Human gene therapy
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1089/104303401750298544
更新日期:2001-07-20 00:00:00
abstract::Guidelines for testing gene therapy products for ecotropic replication-competent retrovirus (Eco-RCR) have not been delineated as they have for amphotropic viruses. To evaluate biologic assays that can detect these viruses, we compared an S(+)/L(-) assay and a marker rescue assay designed specifically for Eco-RCR dete...
journal_title:Human gene therapy
pub_type: 杂志文章
doi:10.1089/104303402760293619
更新日期:2002-09-20 00:00:00
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journal_title:Human gene therapy
pub_type: 杂志文章,评审
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更新日期:2013-05-01 00:00:00
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journal_title:Human gene therapy
pub_type: 杂志文章,评审
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更新日期:2017-11-01 00:00:00
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journal_title:Human gene therapy
pub_type: 杂志文章
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journal_title:Human gene therapy
pub_type: 杂志文章
doi:10.1089/hum.1994.5.7-853
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journal_title:Human gene therapy
pub_type: 杂志文章,评审
doi:10.1089/hum.2017.236
更新日期:2018-05-01 00:00:00
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journal_title:Human gene therapy
pub_type: 杂志文章,多中心研究
doi:10.1089/hum.2014.109
更新日期:2015-03-01 00:00:00
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journal_title:Human gene therapy
pub_type: 杂志文章
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更新日期:1998-10-10 00:00:00
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journal_title:Human gene therapy
pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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journal_title:Human gene therapy
pub_type: 杂志文章
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更新日期:2013-07-01 00:00:00
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journal_title:Human gene therapy
pub_type: 杂志文章
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更新日期:2000-03-20 00:00:00
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journal_title:Human gene therapy
pub_type: 杂志文章
doi:10.1089/hum.2018.062
更新日期:2019-02-01 00:00:00
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journal_title:Human gene therapy
pub_type: 杂志文章
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