Abstract:
:All biological platforms for the manufacture of biopharmaceutical proteins produce an initially turbid extract that must be clarified to avoid fouling sensitive media such as chromatography resins. Clarification is more challenging if the feed stream contains large amounts of dispersed particles, because these rapidly clog the filter media typically used to remove suspended solids. Charged polymers (flocculants) can increase the apparent size of the dispersed particles by aggregation, facilitating the separation of solids and liquids, and thus reducing process costs. However, many different factors can affect the behavior of flocculants, including the pH and conductivity of the medium, the size and charge distribution of the particulates, and the charge density and molecular mass of the polymer. Importantly, these properties can also affect the recovery of the target protein and the overall safety profile of the process. We therefore used a design of experiments approach to establish reliable predictive models that characterize the impact of flocculants during the downstream processing of biopharmaceutical proteins. We highlight strategies for the selection of flocculants during process optimization. These strategies will contribute to the quality by design aspects of process development and facilitate the development of safe and efficient downstream processes for plant-derived pharmaceutical proteins.
journal_name
Bioengineeredjournal_title
Bioengineeredauthors
Buyel JF,Fischer Rdoi
10.4161/bioe.28061keywords:
["design of experiments","filtration and clarification","model building","particle flocculation","plant-produced biopharmaceuticals","process robustness"]subject
Has Abstractpub_date
2014-03-01 00:00:00pages
138-42issue
2eissn
2165-5979issn
2165-5987pii
28061journal_volume
5pub_type
杂志文章相关文献
文献大全abstract::Heparin is the most widely used pharmaceutical to control blood coagulation in modern medicine. A health crisis that took place in 2008 led to a demand for production of heparin from non-animal sources. Since Chinese hamster ovary (CHO) cells are capable of producing heparan sulfate (HS), a related polysaccharide natu...
journal_title:Bioengineered
pub_type: 评论,杂志文章
doi:10.4161/bioe.20902
更新日期:2012-07-01 00:00:00
abstract::N-acetylglucosamine (GlcNAc), the monomer of chitin and constituent of bacterial peptidoglycan, is a preferred carbon and nitrogen source for streptomycetes. Recent studies have revealed new functions of GlcNAc in nutrient signaling of bacteria. Exposure to GlcNAc activates development and antibiotic production of Str...
journal_title:Bioengineered
pub_type: 评论,杂志文章
doi:10.4161/bioe.21371
更新日期:2012-09-01 00:00:00
abstract::Adeno-Associated Virus (AAV) has been widely used for in vivo study and preclinical therapy due to its ability to mediate long-term transgene expression, its lack of pathogenicity and low immunogenicity. It has been found that AAV has more than ten serotypes, which each transfect certain types of cells in the viral in...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.22293
更新日期:2013-03-01 00:00:00
abstract::The baculovirus/insect cell system has proven to be a powerful tool for the expression of eukaryotic proteins. Therapeutics, especially in the field of vaccinology, are often composed of several different protein subunits. Conventional baculoviral expression schemes largely lack efficient strategies for simultaneous m...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.4161/bioe.22327
更新日期:2013-03-01 00:00:00
abstract::Tuo et al. (2012) demonstrated tumor necrosis factor-inducible gene 6 recombinant protein (TSG-6) arrest of focal retinal lesions on a Ccl2 and Cx3cr1 double deficient mouse (DKO) on rd8 background (hereon referred to as DKO rd8). DKO rd8, a model of focal retinal degeneration with earlier onset and higher penetrance ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.22949
更新日期:2013-05-01 00:00:00
abstract::Enteric infections account for high morbidity and mortality and are considered to be the fifth leading cause of death at all ages worldwide. Seventy percent of all enteric infections are foodborne. Thus significant efforts have been directed toward the detection, control and prevention of foodborne diseases. Many anti...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.4161/bioe.23574
更新日期:2013-11-01 00:00:00
abstract::Most essential activities in eukaryotic cells are catalyzed by large multiprotein assemblies containing up to ten or more interlocking subunits. The vast majority of these protein complexes are not easily accessible for high resolution studies aimed at unlocking their mechanisms, due to their low cellular abundance an...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.22966
更新日期:2013-09-01 00:00:00
abstract::Tools for strain engineering with predictable outcome are of crucial importance for the nascent field of synthetic biology. The success of combining different DNA biological parts is often restricted by poorly understood factors deriving from the complexity of the systems. We have previously identified variants for di...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.24703
更新日期:2013-11-01 00:00:00
abstract::Phenylpropanoids and phenylpropanoid-derived phenolic compounds such as flavonoids, anthocyanins, or stilbenes are secondary plant metabolites which serve as pigments and scent compounds or provide protection against environmental stress. Due to their antioxidant properties they also have been widely recognized for th...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.23885
更新日期:2013-11-01 00:00:00
abstract::Whole cell biotransformation systems with enzyme cascading increasingly find application in biocatalysis to complement or replace established chemical synthetic routes for production of, e.g., fine chemicals. Recently, we established an Escherichia coli whole cell biotransformation system for reductive amination by co...
journal_title:Bioengineered
pub_type: 评论,杂志文章
doi:10.4161/bioe.27151
更新日期:2014-01-01 00:00:00
abstract::Previous studies have examined different strategies for siRNA delivery with varying degrees of success. These include use of viral vectors, cationic liposomes, and polymers. Several copolymers were designed and synthesized based on blocks of poly(ethylene glycol) PEG, poly(propylene glycol) PPG, and poly(l-lysine). Th...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.27339
更新日期:2014-01-01 00:00:00
abstract::Experimental techniques allow engineering of biological systems to modify functionality; however, there still remains a need to develop tools to prioritize targets for modification. In this study, agent-based modeling (ABM) was used to build stochastic models of complexed and non-complexed cellulose hydrolysis, includ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.29160
更新日期:2014-07-01 00:00:00
abstract::The DNA sequence upstream of the glyceraldehyde 3-phosphate dehydrogenase gene (gap) of various strains of bifidobacteria is used in a number of vector systems for homologous and heterologous expression in this group of bacteria. To date none of the bifidobacterial gap promoters (Pgap) have been verified experimentall...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.34423
更新日期:2014-01-01 00:00:00
abstract::Itaconic acid is an important building block for the chemical industry. Currently, Aspergillus terreus is the main organism used for itaconic acid production. Due to the enormous citric acid production capacity of Aspergillus niger, this host is investigated as a potential itaconic acid production host. Several strate...
journal_title:Bioengineered
pub_type: 评论,杂志文章
doi:10.4161/bioe.29936
更新日期:2014-09-01 00:00:00
abstract::Ashbya gossypii is a filamentous fungus that naturally overproduces riboflavin. Indeed, engineered strains are currently used for the industrial production of riboflavin, replacing the chemical synthesis processes formerly used. The utilization of A. gossypii for biotechnological applications affords significant advan...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2015.1011525
更新日期:2015-01-01 00:00:00
abstract::Yeasts are widely used for the production of heterologous proteins. Improving the expression of such proteins is a top priority for pharmaceutical and industrial applications. N-Glycosylation, a common form of protein modification in yeasts, facilitates proper protein folding and secretion. Accordingly, our previous s...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2015.1011031
更新日期:2015-01-01 00:00:00
abstract::Alginate lyases catalyze the degradation of alginate, a complex copolymer of α-L-guluronate and its C5 epimer β-D-mannuronate. The enzymes have been isolated from various kinds of organisms with different substrate specificities, including algae, marine mollusks, marine and terrestrial bacteria, and some viruses and f...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.1080/21655979.2015.1030543
更新日期:2015-01-01 00:00:00
abstract::The skin tissue has been shown to behave like a nonlinear anisotropic material. This study was aimed to employ a constitutive fiber family equation to characterize the nonlinear anisotropic mechanical behavior of the rat and mice skin tissues in different anatomical locations, including the abdomen and back, using his...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2015.1036202
更新日期:2015-01-01 00:00:00
abstract::D-Amino acid oxidase (DAO) is a flavin enzyme that catalyzes the oxidative deamination of d-amino acids. This enzyme has been studied extensively both biochemically and structurally as a model for the oxidase-dehydrogenase class of flavoproteins. This enzyme also has various applications, such as the determination of ...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.1080/21655979.2015.1052917
更新日期:2015-01-01 00:00:00
abstract::The review centers on the human gastrointestinal tract; focusing first on the bacterial stress responses needed to overcome the physiochemical defenses of the host, specifically how these stress survival strategies can be used as targets for alternative infection control strategies. The concluding section focuses on r...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.1080/21655979.2015.1089368
更新日期:2015-01-01 00:00:00
abstract::This addendum to "Novel application of bacteriophage for controlling foaming in wastewater treatment plant- an eco-friendly approach " includes characteristics of the phages NOC1, NOC2 and NOC3 not discussed in the previous paper. The phage adsorption and host interaction properties, their sensitivity to pH and temper...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2015.1134066
更新日期:2016-01-01 00:00:00
abstract::Global sustainable development depends on, at least partially, the sustainable development of crop reproduction that provides food, cloth and bioenergy as well certain drugs. During thousands of years of human history, many crops have been domesticated for feeding the world. Perfectly, in the past 2 decades, scientist...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1141838
更新日期:2016-01-01 00:00:00
abstract::Recombinant protein subunit vaccines are formulated using defined protein antigens that can be produced in heterologous expression systems. The methylotrophic yeast Pichia pastoris has become an important host system for the production of recombinant subunit vaccines. Although many basic elements of P. pastoris expres...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.1080/21655979.2016.1191707
更新日期:2016-04-01 00:00:00
abstract::Lipases can catalyze the hydrolysis of glycerol, esters and long chain fatty acids. A lipase producing isolate M35-15 was screened and identified as Thalassospira permensis using 16S rRNA gene sequence analysis. To our knowledge this is the first report on Thalassospira permensis producing lipases. In this paper the o...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1197713
更新日期:2016-09-02 00:00:00
abstract::This study analyzed multiple biokinetic models using a dynamic water phantom. The phantom was custom-made with acrylic materials to model metabolic mechanisms in the human body. It had 4 spherical chambers of different sizes, connected by 8 ditches to form a complex and adjustable water loop. One infusion and drain po...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1197738
更新日期:2016-09-02 00:00:00
abstract::Thermal denaturation of lysozymes was studied as a function of protein concentration, phosphate buffer concentration, and scan rate using differential scanning calorimetry (DSC), which was then analyzed by the isoconversional method. The results showed that lysozyme thermal denaturation was only slightly affected by t...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1197629
更新日期:2016-07-03 00:00:00
abstract::Ethanol production from whey powder was investigated by using free as well as alginate immobilized E. coli and E. coli expressing Vitreoscilla hemoglobin (VHb) in both shake flask and fermenter cultures. Media with varying levels of whey (lactose contents of 3%, 5%, 8% or 15%) and yeast extract (0.3% or 0.5%) were eva...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1218581
更新日期:2017-03-04 00:00:00
abstract::The objective of this study was to comparatively evaluate 3 different sinus lift tools, namely umbrella-shaped sinus lift curette YSL-04, our recently designed probe-improved sinus lift curettes, and our newly invented elevator 014, using our previous developed goat ex vivo models for direct visualizing the effectiven...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1228497
更新日期:2017-07-04 00:00:00
abstract:BACKGROUND:Survivin has been shown to play an important role in cancer pathogenesis. However, its role in cervical cancer development is still controversial. This study was performed to evaluate the clinical significance of survivin expression in cervical cancer. METHODS:Search of some online electronic databases was ...
journal_title:Bioengineered
pub_type: 杂志文章,meta分析
doi:10.1080/21655979.2016.1252879
更新日期:2017-09-03 00:00:00
abstract::The genetic engineering of plants offers a revolutionary advance for crop improvement, and the incorporation of transgenes into crop species can impart new traits that would otherwise be difficult to obtain through conventional breeding. Transgenes introduced into plants, however, can only be useful when bred out to f...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.1080/21655979.2016.1276679
更新日期:2017-09-03 00:00:00