Establishing paediatric reference intervals for thyroid function tests in Croatian population on the Abbott Architect i2000.

Abstract:

Introduction:Evaluation of thyroid function is often requested and therefore defining paediatric reference intervals (RIs) is of vital importance. Currently, there is a distinct lack of paediatric RIs for thyroid function tests in Croatia. Thus, we established RIs for thyroid stimulating hormone (TSH), total triiodothyronine (TT3), total thyroxine (TT4), free triiodothyronine (FT3) and free thyroxine (FT4) in the Croatian paediatric population. Materials and methods:Reference intervals were calculated from 397 apparently healthy children, aged from 2 days to < 19 years. Serum samples were analysed for thyroid function tests on the Abbott Architect i2000. Age- and sex-specific 95% RIs with 90% confidence intervals were established according to Clinical and Laboratory Standards Institute guidelines. To express the magnitude of sex and age variation, standard deviation ratio (SDR) was calculated using two-level nested ANOVA. The criterion for considering partitioning reference values was set to SDR > 0.3. Results:All thyroid function tests required age partitioning, confirmed by SDR above 0.3. There was no need for sex partitioning, confirmed by SDR below 0.3. Still, FT3 was partitioned due to visually noticeable sex related difference for the oldest group (12 years to < 19 years). Conclusion:This is the first study to establish RIs for thyroid function tests in the Croatian paediatric population. We propose RIs for widely used Abbott platform, thus giving laboratories method- and population-specific paediatric RIs for thyroid function tests that should improve clinical test interpretation.

journal_name

Biochem Med (Zagreb)

journal_title

Biochemia medica

authors

Bokulić A,Zec I,Marijančević D,Goreta S,Požgaj Šepec M,La Grasta Sabolić L

doi

10.11613/BM.2021.030702

keywords:

["immunoassay","paediatric","reference values","standard deviation ratio","thyroid function tests"]

subject

Has Abstract

pub_date

2021-10-15 00:00:00

pages

030702

issue

3

eissn

1330-0962

issn

1846-7482

pii

bm-31-3-030702

journal_volume

31

pub_type

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