Comparison of Freelite and N-Latex serum free light chain assays: a critical review.

Abstract:

Introduction:The measurement of serum free light chain (FLC) represents a fundamental aspect on the assessment of patients with monoclonal gammopathies (MG). Different analytical methods for FLC have become available with the possibility to obtain different value with a substantial impact on the assessment of patients with MG. This study aimed to evaluate FLC results obtained with two different assays and how the difference value obtained can impact in the patient's assessment. Materials and methods:Ninety-three patient serum samples that underwent analysis for FLC with two different methods, Serum Freelite (The Binding Site, Birmingham, UK) and N-Latex FLC (Siemens, Marburg, Germany), were included in this retrospective study. Statistical analysis was performed to evaluate correlation, difference, and the grade of concordance between the results obtained with the two methods. Results:Significant statistical differences between the results obtained from the two methods were found (P < 0.05). A good correlation was found (0.99 for κ FLC, 0.95 for λ FLC, and 0.94 for the κ/λ ratio, respectively). We found a weighted kappa value of 0.65 for κ/λ ratio, 0.65 for λ FLC and 0.90 for κ FLC. A positive bias found with the Bland-Altman plot mirrors overestimation of κ FLC and κ/λ ratio with Freelite compared to N-Latex, whilst a negative bias underscores underestimation of λ FLC by Freelite compared to N-Latex. Conclusion:Although in general the concordance between Freelite and N-Latex appears satisfactory, several discrepancies could be evidenced and consequently the two assays are not interchangeable.

journal_name

Biochem Med (Zagreb)

journal_title

Biochemia medica

authors

Daves M,Piccin A,Roccaforte V,Lippi G

doi

10.11613/BM.2021.030701

keywords:

["analytical techniques and equipment","comparison","free light chain","immunoassays","monoclonal gammopathies"]

subject

Has Abstract

pub_date

2021-10-15 00:00:00

pages

030701

issue

3

eissn

1330-0962

issn

1846-7482

pii

bm-31-3-030701

journal_volume

31

pub_type

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