Abstract：
:Recently, antibiotic resistant has become a serious public health concern, which warrants new generations of antibiotics to be developed. Pharmacodynamic evaluation is crucial in drug discovery processes. Despite numerous advanced imaging systems are available nowadays, technologies for the sensitive in vivo diagnosis of bacterial infections and direct visualization of drug efficacy are yet to be developed. In this study, we have developed novel near-infrared (NIR) fluorogenic probes. These probes are dark in solution but highly fluorescent when bound to the cognate reporter, fluorogen-activating protein (FAP). We established the in vivo bacterial infection model using FAP_dH6.2 recombinantly expressed E. coli and applied this NIR fluoromodule-based system for diagnosing bacterial infections and monitoring disease progressions and its responses to a type of antibiotics through classic mechanism of membrane lysis. This NIR fluoromodule-based system will discover new information on bacterial infections and identify newer antibacterial entities.
journal_name
Talantajournal_title
Talantaauthors
Wang X,Wang Q,Zhang Q,Han X,Xu S,Yin D,Hu HYdoi
10.1016/j.talanta.2021.122610keywords:
["Bacterial infections","Fluorogen-activating protein","Fluorogenic probes","In Vivo Imaging","Near-infrared"]subject
Has Abstractpub_date
2021-10-01 00:00:00pages
122610eissn
0039-9140issn
1873-3573pii
S0039-9140(21)00531-2journal_volume
233pub_type
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