Abstract:
:MicroRNAs are short, non-coding RNA sequences involved in gene expression regulation. Quantification of miRNAs in biological fluids involves time consuming and laborious methods such as Northern blotting or PCR-based techniques. Molecular beacons (MB) are an attractive means for rapid detection of miRNAs, although the need for sophisticated readout methods limits their use in research and clinical settings. Here, we introduce a novel method based on delayed electrophoretic mobility, as a quantitative means for detection of miRNAs-MB hybridization. Upon hybridization with the target miRNAs, MB form a fluorescent duplex with reduced electrophoretic mobility, thus bypassing the need for additional staining. In addition to emission of light, the location of the fluorescent band on the gel acts as an orthogonal validation of the target identity, further confirming the specificity of binding. The limit of detection of this approach is approximately 100 pM, depending on the MB sequence. The method is sensitive enough to detect specific red blood cell miRNAs molecules in total RNA, with single nucleotide specificity. Altogether, we describe a rapid and affordable method that offers sensitive detection of single-stranded small DNA and RNA sequences.
journal_name
Biosens Bioelectronjournal_title
Biosensors & bioelectronicsauthors
Oliveira-Jr GP,Barbosa RH,Thompson L,Pinckney B,Murphy-Thornley M,Lu S,Jones J,Hansen CH,Tigges J,Wong WP,Ghiran ICdoi
10.1016/j.bios.2021.113307keywords:
["Electrophoretic mobility","Gel electrophoresis","Molecular beacons","microRNA (miRNA)"]subject
Has Abstractpub_date
2021-10-01 00:00:00pages
113307eissn
0956-5663issn
1873-4235pii
S0956-5663(21)00344-4journal_volume
189pub_type
杂志文章abstract::Herein, an ultra-sensitive alkaline phosphatase (ALP) sensing strategy is developed by target-induced transcription amplification to trigger the trans-cleavage activity of Cas13a (TITAC-Cas). A double-stranded DNA duplex integrating a T7 promoter with 5'-phosphate and a transcription template (5'P-dsDNA) serves as the...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113281
更新日期:2021-04-27 00:00:00
abstract::We describe a miniaturized field-deployable biosensor module, designed to function as an element in a sensor network for standoff monitoring and mapping of environmental hazards. The module harbors live bacterial sensor cells, genetically engineered to emit a bioluminescent signal in the presence of preselected target...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113253
更新日期:2021-04-15 00:00:00
abstract::The urge to meet the ever-growing needs of sensing technology has spurred research to look for new alternatives to traditional analytical methods. In this scenario, the glucometer is the flagship of commercial electrochemical sensing platforms, combining selectivity, reliability and portability. However, other types o...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章,评审
doi:10.1016/j.bios.2021.113242
更新日期:2021-04-17 00:00:00
abstract::Metamaterial-inspired biosensors have been extensively studied recently years for fast and low-cost THz detection. However, only the variation of the resonance frequency has been closely concerned in such sensors so far, whiles the magnitude variation, which also provide important information of the analyte, has not b...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113241
更新日期:2021-04-22 00:00:00
abstract::Gradient properties facilitate the correlation of chemical and physical features of particles on the structure and adherent fate. Herein, performance enhancement is explored by particle gradient assembly patterning (PGAP) formed with magnetic field gradient (MFG) by magnetolithography (ML) in the electrochemiluminesce...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113240
更新日期:2021-07-01 00:00:00
abstract::Epithelial-mesenchymal transition (EMT) is closely related to tumor metastasis and invasion. Thereinto, mesenchymal tumor mitochondria are the critical target for tumor inhibition. Therefore, real-time in vivo monitoring of EMT as well as inhibiting mesenchymal tumor mitochondria is of great diagnosis and therapy sign...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113235
更新日期:2021-04-19 00:00:00
abstract::The widespread use of herbicides in agriculture and gardening causes environmental and safety issues such as water pollution. Thus, efficient and convenient analysis of the levels of herbicide residues is of significant importance. Here, we employed 3D-printing to design a multiplex immunosensor for simultaneous detec...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113238
更新日期:2021-04-10 00:00:00
abstract::Although conventional skin-attachable electronics exhibit good functionalities, their direct attachment (without any adhesive) to human skin with sufficient conformal contact is challenging. Herein, all-solution-processed on-skin electronics based on self-reconfigurable high-weight-per- volume-gelatin (HWVG) film cons...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113231
更新日期:2021-04-15 00:00:00
abstract::Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters the cells through the binding of its spike protein (S-protein) to the cell surface-expressing angiotensin-converting enzyme 2 (ACE2). Thus, inhibition of S-protein-ACE2 binding may impede SARS-CoV-2 cell entry and attenuate the progression of Coronavi...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113213
更新日期:2021-07-01 00:00:00
abstract::Molecular diagnostics are vital for the identification, prevention, and treatment of numerous diseases and are of particular demand in point-of-care (POC) settings. Nevertheless, most reported biosensors based on the CRISPR-Cas system have focused on nucleic-acid targets. Here, we report a versatile diagnostic strateg...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113196
更新日期:2021-07-01 00:00:00
abstract::SARS-CoV-2 RNA is identified as a pivotal player to bolster energizing zones of COVID-19 detection. Herein, we develop a rapid and unamplified nanosensing platform for detection of SARS-CoV-2 RNA in human throat swab specimens. A gold nanoparticle (AuNP)-decorated graphene field-effect transistor (G-FET) sensor was fa...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113206
更新日期:2021-07-01 00:00:00
abstract::In this study, we firstly propose a novel smartphone-assisted visualization SNP genotyping method termed competitive activation cross amplification (CACA). The mutation detection strategy depends on the ingenious design of both a start primer and a verification probe with ribonucleotide insertion through competitive c...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113200
更新日期:2021-07-01 00:00:00
abstract::α-Glucosidase (α-Glu) and its inhibitors play critical roles in diabetes therapy. Herein, a simple and ultra-sensitive fluorescence sensing approach was fabricated for α-Glu activity monitoring and natural inhibitor screening by electrostatically confining negatively charged glutathione-capped copper nanoclusters (GSH...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113198
更新日期:2021-06-15 00:00:00
abstract::The current clinically available multiplex molecular diagnostic technologies are difficult to apply to onsite diagnostic platforms due to their large and sophisticated instrumentation, long amplification times and limited number of simultaneous detections. We developed a plasmonic isothermal recombinase polymerase amp...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113167
更新日期:2021-06-15 00:00:00
abstract::Diabetes mellitus is a chronic illness in the United States affecting nearly 120 million adults, as well as increasing in children under the age of 18. Diabetes was also the 7th leading cause of death in the United States with 270 K deaths in 2017. Diabetes is best managed by tight glycemic control, as achieving near-...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113054
更新日期:2021-06-01 00:00:00
abstract::Photocathode-based assay is anti-interference for real sample detection. Photocathode produces low photocurrent signal and gives rise to poor sensitivity. Herein, a novel cathode photoelectrochemical (CPEC) sensing platform based on Ag-AgI-CNTs as photocathode material and K3[Fe(CN)6] as photoelectron acceptor was est...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113152
更新日期:2021-06-01 00:00:00
abstract::Proteins are generally detected as biomarkers for tracing or determining various disorders in organisms. Biomarker proteins can be tracked in samples with various origins and in different concentrations, revealing whether an organism is in a healthy or unhealthy state. In regard to detection, electrochemical biosensor...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章,评审
doi:10.1016/j.bios.2021.113087
更新日期:2021-05-15 00:00:00
abstract::Uric acid analysis is extremely important for gout prognosis, diagnosis and treatment. Previous technologies either lack specificity or exhibit poor performance, and thus could not meet the need of Point-of-Care (POC) uric acid monitoring. Here we present for the first time, a novel electrochemical biosensor based on ...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113082
更新日期:2021-05-01 00:00:00
abstract::Efficient and multiple analysis of receptor protein dimers is highly necessary, due to their important role in the occurrence and development of cancer. Herein, we report a turn-on strategy to visualize human epidermal growth factor receptor (HER) dimers on cell surfaces. By taking advantages of specific aptamer recog...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113064
更新日期:2021-05-01 00:00:00
abstract::Herein, we report a novel dual-quenching electrochemiluminescence (ECL) immunosensor for detecting protein induced by vitamin K absence or antagonist-II (PIVKA-II) based on ECL resonance energy transfer (ECL-RET). In this protocol, self-accelerated ECL hybrids of CeO2 and Au nanoparticles functionalized g-C3N4 nanoshe...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113059
更新日期:2021-05-01 00:00:00
abstract::Stem cell-based therapies have recently emerged to treat various incurable diseases and disorders. Types of stem cell-derived cells and their functions should be intensively analyzed before therapy. However, current pre-treatment steps for biological analysis are mostly destructive. Here, we report a novel optical met...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113018
更新日期:2021-04-15 00:00:00
abstract::The association of mortality with the early humoral response to SARS-CoV-2 infection within the first few days after onset of symptoms (DAOS) has not been thoroughly investigated partly due to a lack of sufficiently sensitive antibody testing methods. Here we report two sensitive and automated testing-on-a-probe (TOP)...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113008
更新日期:2021-04-15 00:00:00
abstract::Klebsiella pneumoniae is an opportunistic pathogen that is responsible for community acquired infections and nosocomial infections. Antibiotic-resistant K. pneumoniae and/or hypervirulent K. pneumoniae are emerging as a serious threat to public health. For the sake of alleviating and conquering current dilemma, discov...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2020.112919
更新日期:2021-04-01 00:00:00
abstract::The competitive endogenous RNA hypothesis is a new mechanism of RNA dialogue, in which circRNA-miRNA interaction (cmRRI) is found to be widely involved in the regulation of gene expression in tumors and other diseases. It is urgent but challenging to develop a convenient and efficient method to study the interaction b...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113032
更新日期:2021-04-15 00:00:00
abstract::Being closely associated with a variety of physiological and pathological processes, matrix metalloproteinases (MMPs) are useful as potential targets for drug therapy and informative markers for disease diagnosis. On the basis of the electrochemically induced grafting of ferrocenyl polymers and the proteolytic cleavag...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.113010
更新日期:2021-04-15 00:00:00
abstract::Recently the use of microRNAs (miRNAs) as biomarkers for a multitude of diseases has gained substantial significance for clinical as well as point-of-care diagnostics. Amongst other challenges, however, it holds the central requirement that the concentration of a given miRNA must be evaluated within the context of oth...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2020.112887
更新日期:2021-04-01 00:00:00
abstract::A novel chemiluminescence (CL) immunosensor array in combination with a dual-signal amplification strategy was developed for rapid and ultrasensitive detection of multiple mycotoxins in herbal medicine. The multi-component immunosensor array was constructed by immobilizing different bovine serum albumin combined mycot...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.112998
更新日期:2021-04-01 00:00:00
abstract::The unique profile of upregulated glycosylation in metastatic cancer cells may form the basis for the development of new biomarkers for the targeting and diagnosis of specific cancers. This study introduces a pancreatic cancer cell-derived exosome detection technology, which is based on the specific binding of lectins...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2021.112980
更新日期:2021-04-01 00:00:00
abstract::In the present study, we upgraded Pyrococcus furiosus Argonaute (PfAgo) mediated nucleic acid detection method and established a highly sensitive and accurate molecular diagnosis platform for the large-scale screening of COVID-19 infection. Briefly, RT-PCR was performed with the viral RNA extracted from nasopharyngeal...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2020.112932
更新日期:2021-04-01 00:00:00
abstract::The worldwide epidemic of novel coronavirus disease (COVID-19) has led to a strong demand for highly efficient immunobinding to achieve rapid and accurate on-site detection of SARS-CoV-2 antibodies. However, hour-scale time-consumption is usually required to ensure the adequacy of immunobinding on expensive large inst...
journal_title:Biosensors & bioelectronics
pub_type: 杂志文章
doi:10.1016/j.bios.2020.112920
更新日期:2021-03-15 00:00:00