Abstract：
OBJECTIVE:A novel locus in the hepatic lipase (LIPC) gene was found to be significantly related to advanced age-related macular degeneration (AMD) in our genome-wide association study. We evaluated its association and interaction with previously identified genetic variants and modifiable factors. METHODS:Participants in the Age-Related Eye Disease Study with advanced AMD (n=545 cases) or no AMD (n=275 controls) were evaluated. AMD status was determined using fundus photography. Covariates included cigarette smoking, body mass index (BMI), and dietary lutein. Individuals were genotyped for the rs10468017 polymorphism in LIPC as well as seven previously identified AMD genetic loci. Unconditional logistic regression analyses were then performed. RESULTS:The TT genotype of the LIPC variant was associated with a reduced risk of AMD, with odds ratios (OR) of 0.50 (95% confidence interval (CI) 0.20-0.90) and p=0.014 for the TT genotype versus the CC genotype, controlling for age, gender, smoking, body mass index (BMI), and nutritional factors. Controlling for seven other AMD genetic variants, the OR was 0.50, 95% (CI 0.20-1.1, p=0.077). The magnitude of the effect was similar for both atrophic and neovascular forms of AMD. Cigarette smoking and higher BMI increased the risk, while higher dietary lutein reduced the risk of advanced AMD, adjusting for genetic variants. There were no significant interactions between LIPC and smoking, BMI, or lutein. There was a possible association between LIPC and complement factor H (CFH) rs1410996, and a possible interaction effect between LIPC and both CFH rs10033900 and the complement factor I (CFI) variants in terms of risk of AMD. CONCLUSIONS:LIPC is associated with reduced risk of advanced AMD, independent of demographic and environmental variables. Both genetic susceptibility and behavioral and lifestyle factors modify the risk of developing AMD.
journal_name
Mol Visjournal_title
Molecular visionauthors
Seddon JM,Reynolds R,Rosner Bsubject
Has Abstractpub_date
2010-11-17 00:00:00pages
2412-24issn
1090-0535pii
259journal_volume
16pub_type
杂志文章相关文献
MOLECULAR VISION文献大全abstract：PURPOSE:To characterize the disease-causing mutations and related phenotypes in two Chinese families with autosomal dominant congenital cataract. METHODS:Family members were clinically characterized by a complete eye examination. Genome-wide linkage screening was performed in Family 1 using a 10K single nucleotide pol...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2007-07-13 00:00:00
abstract：PURPOSE:The purpose of the study was to compare and analyze the composition of crystallin species that exist in the water insoluble-urea soluble (WI-US) and water insoluble-urea insoluble (WI-UI) protein fractions of a human cataractous lens and an age-matched normal lens. METHODS:The water soluble (WS) and water inso...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2007-09-14 00:00:00
abstract：PURPOSE:The replacement of glucose by sorbitol in growth medium causes selection of astroglial cells from heterogeneous primary cultures derived from the brains of newborn mice. The present study was undertaken to investigate the effects of sorbitol on in vitro selection of Müller glial precursors from expanded late re...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2006-12-20 00:00:00
abstract：PURPOSE:To clarify the role of bone morphogenetic proteins (BMP-2,-4,-5) in sclera remodeling during myopia induction and their effect on sclera fibroblasts in cell culture. METHODS:Reverse transcription and polymerase chain reaction (RT-PCR) as well as immunofluorescence were used to detect the expression of the BMPs...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2011-03-08 00:00:00
abstract：PURPOSE:Myocilin, the product of the GLC1A locus in humans, has been associated with primary open angle glaucoma (POAG). Mutations in myocilin exon 3 correlate with age of onset and severity of POAG. Myocilin has been under investigation in experimentally induced glaucoma in rats, mice, and monkeys, however no animal m...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2006-11-17 00:00:00
abstract：PURPOSE:Retinal degeneration has been associated with iron accumulation in age-related macular degeneration (AMD), and in several rodent models that had one or several iron regulating protein impairments. We investigated the iron concentration and the protective role of human transferrin (hTf) in rd10 mice, a model of ...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2010-12-08 00:00:00
abstract：PURPOSE:The purpose of this study was to measure the effects of mutations on the retinol binding capability of human Repeat 1 of interphotoreceptor retinoid-binding protein (IRBP). First, we predicted important functional amino acids by several computer programs. We also noted the lack of shared functions between Tail-...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2000-04-07 00:00:00
abstract：PURPOSE:To analyze the potential association of programmed cell death 1 (PDCD1) with Vogt-Koyanagi-Harada (VKH) syndrome in a Chinese Han population. METHODS:Three single nucleotide polymorphism (SNPs), PD-1.3G/A, PD-1.5C/T, and PD-1.6G/A, were genotyped in 247 VKH patients and 289 age-, sex-, and ethnically-matched h...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：PURPOSE:The dopamine (DA) system in the retina is critical to normal visual development as lack of retinal DA signaling may contribute to myopic development. The involvement of DA in myopic development is complex and may be different between form deprivation and hyperopic defocus. This study evaluated effects of a non-...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
abstract：PURPOSE:The use of anti-vascular endothelial growth factor (anti-VEGF) therapy, with drugs such as ranibizumab and bevacizumab, to treat neovascular age-related macular degeneration (nAMD) produces an effective but widely variable response. Identifying markers that predict differentiated response could serve as a valua...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：PURPOSE:Defects in the development and physiology of the lens can result in cataracts (opacification of the lens), which are currently treatable only by surgical removal. The lens is also an excellent system for understanding fundamental biological processes such as cellular differentiation and ageing. Here, microarray...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2003-08-22 00:00:00
abstract：PURPOSE:To investigate the inhibitory effects of anti-mouse interleukin-17 (IL-17) monoclonal antibody (mAb) in high-responder corneal allograft rejection. METHODS:C57BL/6 or BALB/c mice corneal grafts were grafted onto BALB/c hosts. The neutralizing mouse IL-17 antibody and isotype control were injected intraperitone...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
abstract：PURPOSE:To determine the role of the recently discovered primary open angle glaucoma (POAG) risk factor mapped to chromosome 7q31 in glaucoma patients from Iowa and to determine the expression pattern of genes in the locus in human eyes. METHODS:A cohort of 545 POAG patients and 297 control subjects from Iowa were gen...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2011-02-08 00:00:00
abstract：PURPOSE:Myocilin is a gene linked to open-angle glaucomas. In this study, the expression and distribution of myocilin in corneal fibroblasts with or without dexamethasone (DEX) treatment were investigated. METHODS:Human corneal fibroblasts were treated with 100 nM DEX for 10-14 days. Immunofluorescence staining for my...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2003-07-03 00:00:00
abstract：PURPOSE:The retinal pigment epithelium (RPE) and choroid comprise a functional unit of the eye that is essential to normal retinal health and function. Here we describe expressed sequence tag (EST) analysis of human RPE/choroid as part of a project for ocular bioinformatics. METHODS:A cDNA library (cs) was made from h...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2002-06-15 00:00:00
abstract：PURPOSE:The clinical phenotype of advanced stage retinopathy of prematurity (ROP, stages 4 and 5) cannot be replicated in an animal model. To dissect the molecular events that can lead up to advanced ROP, we examined subretinal fluid (SRF) and surgically dissected retrolental membranes from patients with advanced ROP t...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2014-06-21 00:00:00
abstract：PURPOSE:To evaluate the tear film characteristics of spontaneous subconjunctival hemorrhage patients by Schirmer test I and tear interferometry. METHODS:Forty-six spontaneous subconjunctival hemorrhage patients and 46 controls were enrolled in the study. Schirmer test I and tear interferometry were performed in all 92...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：PURPOSE:Uveitic inflammatory injury can cause irreversible visual loss; however, no single animal model recapitulates all the characteristics of human uveitis. Ultraviolet radiation (UVR) is one of the risk factors for uveitis, but the role of UVR in the pathogenesis of uveitic injury is unclear. The aim of this study ...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2017-04-12 00:00:00
abstract：PURPOSE:AND-34/BCAR3 (Breast Cancer Anti-Estrogen Resistance 3) associates with the focal adhesion adaptor protein, p130CAS/BCAR1. Expression of AND-34 regulates epithelial cell growth pattern, motility, and growth factor dependence. We sought to establish the effects of the loss of AND-34 expression in a mammalian org...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：PURPOSE:To determine comparative effects of ultraviolet (UV)-A irradiation on structural and functional properties of wild type (WT) alphaB-crystallin and its three deamidated mutant proteins (alphaB-Asn78Asp, alphaB-Asn146Asp, and alphaB-Asn78/146Asp). METHODS:Three deamidated mutants previously generated from recomb...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2008-02-01 00:00:00
abstract：PURPOSE:This study investigated the relationship between B lymphoma Mo-MLV insertion region 1 (BMI-1)--a polycomb protein for stem cell self-renewal and proliferation--and the clinicopathological parameters of human retinoblastomas, including differentiation status and retinal tissue invasion, as well as the effects of...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：PURPOSE:To screen the exons of the genes encoding the beta3-subunit (GNB3) and gammac-subunit (GNGT2) of cone transducin for mutations in a large number of unrelated patients with various forms of inherited retinal disease including cone dystrophy, cone-rod dystrophy and macular dystrophy. METHODS:Exons of the two gen...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：1998-09-17 00:00:00
abstract：PURPOSE:To determine whether docosahexaenoic acid can protect against hereditary retinal degenerations in transgenic mice expressing the V20G, P23H, and P27L (VPP) rhodopsin mutations. METHODS:Female transgenic mice expressing the VPP rhodopsin mutation, known to cause a retinal degeneration, were bred to male transge...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2010-08-18 00:00:00
abstract：PURPOSE:Autosomal dominant early-onset long anterior zonules (LAZs) and late-onset retinal degeneration (L-ORD) in humans are associated with the S163R mutation of the complement 1q-tumor necrosis factor related protein-5 (CTRP5) gene. For using the pig as an L-ORD model for the study of pathology, we cloned, character...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：PURPOSE:The purpose of these studies was to develop a method to track intraocular injections. METHODS:Retinal pigment epithelial (RPE) cells, purified from adult mouse eyes, were incubated with superparamagnetic microbeads (Dynabeads, 4.5 microm) coated with bovine serum albumin to verify that they could phagocytose t...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2005-04-15 00:00:00
abstract：PURPOSE:In addition to chronic hyperglycemia, there is increasing evidence that genetic factors may be important in the development of diabetes retinopathy (DR). Specifically, polymorphisms of the endothelial nitric oxide synthase gene (eNOS) have been reported to be associated with multiple health conditions including...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2007-11-26 00:00:00
abstract：PURPOSE:Analysis of mutant mouse strains and linkage analysis with human families have both demonstrated that mutations influencing the podosomal adaptor protein SH3 and PX domains 2B (SH3PXD2B) can result in a congenital form of glaucoma. Here, we use immunohistochemistry to describe localization of the SH3PXD2B prote...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：PURPOSE:Fibroblast growth factor 2 (FGF-2) induces endothelial-mesenchymal modulation in corneal endothelial cells, including stimulation of cell proliferation and cell shape change and induction of fibrillar collagen. In the present study, we investigated whether FGF-2 uses distinct signaling pathways for individual b...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：1998-10-27 00:00:00
abstract：PURPOSE:The purpose of the current study was to examine the ocular pathogenesis and immune reaction in mice after intravitreal dispase injection. METHODS:Three microliters of dispase at a concentration of 0.2 U/μl were injected into the vitreal cavities of 4-6-week-old mice. Hematoxylin and eosin staining, immunofluor...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：PURPOSE:This research involves the use of a laser scanning instrument to evaluate the formation of cold cataracts in young and older bovine lenses. METHODS:Bovine lenses from 18 (n=14) week old calf eyes and approximately 10 (n=7) year old animals were extracted in a sterile environment. The lenses were placed in a sp...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2004-03-08 00:00:00
