Peptidylarginine deiminase type 2 is over expressed in the glaucomatous optic nerve.

Abstract:

PURPOSE:To determine levels of Peptidyl arginine deiminase 2 (PAD2) and its product protein-bound citrulline in cadaver eyes that suffered from normal tension glaucoma (NTG) compared to primary open angle glaucoma (POAG), and controls. METHODS:Western analysis, ELISA, and immunohistochemical analysis were performed with human tissues. RESULTS:We report over expression of PAD2 and higher levels of its product protein-bound citrulline in the optic nerve of normal tension glaucoma patients (NTG). CONCLUSIONS:This is the first report demonstrating that like in POAG, NTG also possesses elevated levels of both PAD2 and protein-bound citrulline.

journal_name

Mol Vis

journal_title

Molecular vision

authors

Cafaro TA,Santo S,Robles LA,Crim N,Urrets-Zavalia JA,Serra HM

subject

Has Abstract

pub_date

2010-08-17 00:00:00

pages

1654-8

issn

1090-0535

pii

178

journal_volume

16

pub_type

杂志文章
  • Retinal degeneration modulates intracellular localization of CDC42 in photoreceptors.

    abstract:PURPOSE:Rho GTPases such as RAS-related C3 botulinum substrate 1 (RAC1) and cell division cycle 42 homolog (S. cerevisiae; CDC42) have been linked to cellular processes including movement, development, and apoptosis. Recently, RAC1 has been shown to be a pro-apoptotic factor in the retina during light-induced photorece...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Heynen SR,Tanimoto N,Joly S,Seeliger MW,Samardzija M,Grimm C

    更新日期:2011-01-01 00:00:00

  • Fine mapping of the keratoconus with cataract locus on chromosome 15q and candidate gene analysis.

    abstract:PURPOSE:To report the fine mapping of the keratoconus with cataract locus on chromosome 15q and the mutational analysis of positional candidate genes. METHODS:Genotyping of two novel microsatellite markers and a single nucleotide polymorphism (SNP) in the critical region of linkage for keratoconus with cataract on 15q...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Dash DP,Silvestri G,Hughes AE

    更新日期:2006-05-12 00:00:00

  • Different tropism of adenoviruses and adeno-associated viruses to corneal cells: implications for corneal gene therapy.

    abstract:PURPOSE:Diseased corneas are potential targets for viral-based gene therapy to normalize (stimulate or inhibit) the expression of specific proteins. The choice of viral vectors is important to achieve optimal effect. The purpose of this study was to compare the tropism to different corneal cells of recombinant adenovir...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Liu J,Saghizadeh M,Tuli SS,Kramerov AA,Lewin AS,Bloom DC,Hauswirth WW,Castro MG,Schultz GS,Ljubimov AV

    更新日期:2008-01-01 00:00:00

  • Age and topographical comparison of telomere lengths in human corneal endothelial cells.

    abstract:PURPOSE:Human corneal endothelium exhibits both age-related and topographical differences in relative proliferative capacity and in senescence characteristics. The purpose of these studies was to compare telomere lengths in human corneal endothelial cells (HCEC) from the central and peripheral areas of corneas from you...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Konomi K,Joyce NC

    更新日期:2007-07-23 00:00:00

  • Age-related changes in the water-soluble lens protein composition of Wistar and accelerated-senescence OXYS rats.

    abstract:PURPOSE:To determine the age-related and the cataract-specific changes in the crystallin composition in lenses of accelerated-senescence OXYS (cataract model) and Wistar (control) rats. METHODS:The water soluble (WS) and insoluble (WIS) fractions of the lens proteins were separated; the identity and relative abundance...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Kopylova LV,Cherepanov IV,Snytnikova OA,Rumyantseva YV,Kolosova NG,Tsentalovich YP,Sagdeev RZ

    更新日期:2011-01-01 00:00:00

  • Polymorphism in the 11q24.1 genomic region is associated with myopia: a comprehensive genetic study in Chinese and Japanese populations.

    abstract:PURPOSE:To evaluate the association of polymorphisms in the 11q24.1 genomic region and the CTNND2 gene with myopia. METHODS:We conducted a comprehensive meta-analysis included 6,954 cases and 9,346 controls. Odds ratios (ORs) were calculated using Carlin's method. Publication bias was assessed using Egger et al.'s app...

    journal_title:Molecular vision

    pub_type: 杂志文章,meta分析

    doi:

    authors: Liu J,Zhang HX

    更新日期:2014-03-21 00:00:00

  • The tear film characteristics of spontaneous subconjunctival hemorrhage patients detected by Schirmer test I and tear interferometry.

    abstract:PURPOSE:To evaluate the tear film characteristics of spontaneous subconjunctival hemorrhage patients by Schirmer test I and tear interferometry. METHODS:Forty-six spontaneous subconjunctival hemorrhage patients and 46 controls were enrolled in the study. Schirmer test I and tear interferometry were performed in all 92...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Liu W,Li H,Qiao J,Tian T,An L,Xing X,Liu A,Ji J

    更新日期:2012-01-01 00:00:00

  • Inhibition by brimonidine of forskolin-induced nitric oxide synthase expression in human ciliary bodies in vitro.

    abstract:PURPOSE:To investigate the mRNA and protein expression of nitric oxide synthase (NOS) in human ciliary bodies in vitro. The effect of the adenylcyclase activator forskolin and/or the alpha2-adrenergic agonist brimonidine (an ocular hypotensive agent that inhibits aqueous humor formation) on NOS mRNA or protein expressi...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Wu R,Yin J,Yao K,Haefliger I

    更新日期:2007-04-02 00:00:00

  • Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity.

    abstract:PURPOSE:Keratoconus (KC) is characterized by progressive vision loss due to corneal thinning and structural abnormalities. It is hypothesized that KC is caused by deregulated collagen levels and collagen fibril-maturating enzyme lysyl oxidase (LOX). Further, it is currently not understood whether the gene expression de...

    journal_title:Molecular vision

    pub_type: 临床试验,杂志文章

    doi:

    authors: Shetty R,Sathyanarayanamoorthy A,Ramachandra RA,Arora V,Ghosh A,Srivatsa PR,Pahuja N,Nuijts RM,Sinha-Roy A,Mohan RR,Ghosh A

    更新日期:2015-01-12 00:00:00

  • Initiation codon mutation in betaB1-crystallin (CRYBB1) associated with autosomal recessive nuclear pulverulent cataract.

    abstract:PURPOSE:To identify the molecular basis for autosomal recessively inherited congenital non-syndromic pulverulent cataracts in a consanguineous family with four affected children. METHODS:An autozygosity mapping strategy using high density SNP microarrays and microsatellite markers was employed to detect regions of hom...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Meyer E,Rahman F,Owens J,Pasha S,Morgan NV,Trembath RC,Stone EM,Moore AT,Maher ER

    更新日期:2009-05-18 00:00:00

  • Predominant rod photoreceptor degeneration in Leber congenital amaurosis.

    abstract:PURPOSE:An unusual retinal vascular morphology in an enucleated eye from a patient with Leber congenital amaurosis (LCA) has been associated with a mutation in AIPL1. The AIPL1 protein is expressed in the pineal gland and retinal photoreceptors. In the retina, AIPL1 is expressed in both developing cone and rod photorec...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: van der Spuy J,Munro PM,Luthert PJ,Preising MN,Bek T,Heegaard S,Cheetham ME

    更新日期:2005-07-22 00:00:00

  • Identification of presumed pathogenic KRT3 and KRT12 gene mutations associated with Meesmann corneal dystrophy.

    abstract:PURPOSE:To report potentially pathogenic mutations in the keratin 3 (KRT3) and keratin 12 (KRT12) genes in two individuals with clinically diagnosed Meesmann corneal dystrophy (MECD). METHODS:Slit-lamp examination was performed on the probands and available family members to identify characteristic features of MECD. A...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Chen JL,Lin BR,Gee KM,Gee JA,Chung DW,Frausto RF,Deng SX,Aldave AJ

    更新日期:2015-12-31 00:00:00

  • Experimental murine myopia induces collagen type Iα1 (COL1A1) DNA methylation and altered COL1A1 messenger RNA expression in sclera.

    abstract:PURPOSE:To investigate whether myopia development is associated with changes of scleral DNA methylation in cytosine-phosphate-guanine (CpG) sites in the collagen 1A1 (COL1A1) promoter and messenger RNA (mRNA) levels following murine form deprivation myopia. METHODS:Fifty-seven C57BL/6 mice (postnatal day 23) were rand...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Zhou X,Ji F,An J,Zhao F,Shi F,Huang F,Li Y,Jiao S,Yan D,Chen X,Chen J,Qu J

    更新日期:2012-01-01 00:00:00

  • Coexpression of nonvisual opsin, retinal G protein-coupled receptor, and visual pigments in human and bovine cone photoreceptors.

    abstract:Purpose:Retinal G protein-coupled receptor (RGR) mRNA is transcribed in the outer nuclear layer of human retinas; however, it is not known whether the RGR gene is expressed in the rod or cone photoreceptors. In this study, we investigate broader expression of the normal RGR isoform in photoreceptors of human and bovine...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Zhang Z,Fong HKW

    更新日期:2018-07-02 00:00:00

  • The polyamidoamine-mediated inhibition of bcl-2 by small hairpin RNA to induce apoptosis in human lens epithelial cells.

    abstract:PURPOSE:To investigate whether apoptosis of human lens epithelial cells (HLECs) can be induced with the polyamidoamine (PAMAM)-mediated inhibition of bcl-2 (b-cell lymphoma 2) by small hairpin RNA (shRNA). METHODS:HLECs (SRA01/04) were transfected with the fifth generation of PAMAM (PAMAM G5) by bcl-2 shRNA. At 24, 48...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Wu XH,Lu Y,Fang YW,Jiang YX

    更新日期:2012-01-01 00:00:00

  • Leukostasis and pigment epithelium-derived factor in rat models of diabetic retinopathy.

    abstract:PURPOSE:Spontaneously diabetic Torii (SDT) rats, an animal model of type 2 diabetes, have a low incidence of neovascular formation and an absence of non-perfused areas in their retinas at the proliferative stage that presents tractional retinal detachment with fibrous proliferation. The aim of this study was to determi...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Matsuoka M,Ogata N,Minamino K,Matsumura M

    更新日期:2007-06-29 00:00:00

  • Review: taurine: a "very essential" amino acid.

    abstract::Taurine is an organic osmolyte involved in cell volume regulation, and provides a substrate for the formation of bile salts. It plays a role in the modulation of intracellular free calcium concentration, and although it is one of the few amino acids not incorporated into proteins, taurine is one of the most abundant a...

    journal_title:Molecular vision

    pub_type: 杂志文章,评审

    doi:

    authors: Ripps H,Shen W

    更新日期:2012-01-01 00:00:00

  • NADH photo-oxidation is enhanced by a partially purified lambda-crystallin fraction from rabbit lens.

    abstract:PURPOSE:In the rabbit lens, high levels of reduced nicotinamide adenine dinucleotide (NADH) can function as a near-ultraviolet light (near-UV) filter, an effect apparently achieved by specific nucleotide binding to lambda-crystallin. The present investigation asks whether lambda-crystallin enhances NADH photo-oxidation...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Bando M,Oka M,Kawai K,Obazawa H,Takehana M

    更新日期:2007-09-18 00:00:00

  • The timing of goniosynechialysis in treatment of primary angle-closure glaucoma combined with cataract.

    abstract:PURPOSE:To compare the clinical effects of phacoemulsification (PHACO) combined with goniosynechialysis (GSL) at different times in the treatment of primary angle-closure glaucoma (PACG) combined with cataract. METHODS:Before surgery, one or more different kinds of anti-glaucoma medicines were used for 24 patients (32...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Yu J,Sun M,Wei Y,Cai X,He C,An X,Ye J

    更新日期:2012-01-01 00:00:00

  • Accumulation of cholesterol and increased demand for zinc in serum-deprived RPE cells.

    abstract:PURPOSE:Having observed that confluent ARPE-19 cells (derived from human RPE) survive well in high-glucose serum-free medium (SFM) without further feeding for several days, we investigated the expression profile of RPE cells under the same conditions. METHODS:Expression profiles were examined with microarray and quant...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Mishra S,Peterson K,Yin L,Berger A,Fan J,Wistow G

    更新日期:2016-12-10 00:00:00

  • Hyperoxia-induced lens damage in rabbit: protective effects of N-acetylcysteine.

    abstract:PURPOSE:To investigate the efficacies of different concentrations of N-acetylcysteine (NAC) in preventing hyperoxia-induced lens opacification and changes to biochemical parameters in organ-cultured rabbit lenses. METHODS:Thirty-six lenses from adult rabbits were divided into the control group (group A), the hyperoxia...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Wang P,Liu XC,Yan H,Li MY

    更新日期:2009-12-31 00:00:00

  • Quantitative measurement of young human eye lens crystallins by direct injection Fourier transform ion cyclotron resonance mass spectrometry.

    abstract:PURPOSE:Human eye lenses at birth are primarily constructed of 12 distinct crystallins and two truncated crystallins. The molecular weights of these 14 proteins vary between about 20,000 and 30,000 Da. The relative amounts of these molecules and their post-synthetic changes with age are of substantial interest in the s...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Robinson NE,Lampi KJ,Speir JP,Kruppa G,Easterling M,Robinson AB

    更新日期:2006-06-21 00:00:00

  • Impact of interleukin-6 promoter polymorphism and serum interleukin-6 level on the acute inflammation and neovascularization stages of patients with Eales' disease.

    abstract:PURPOSE:To evaluate the role of interleukin-6 (IL-6) in the inflammatory and proliferative stages of Eales' disease (ED) and to determine the influence of IL-6-174G/C polymorphism in the IL-6 and IL-6-regulated protein expression, as well as the development of ED. METHODS:One hundred and twenty-one patients diagnosed ...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Sen A,Paine SK,Chowdhury IH,Mukherjee A,Choudhuri S,Saha A,Mandal LK,Bhattacharya B

    更新日期:2011-01-01 00:00:00

  • Down-regulation of GRP78 in human glaucomatous trabecular meshwork cells.

    abstract:PURPOSE:Since the 78 kDa glucose-regulated protein (GRP78) is a key marker of endoplasmic reticulum (ER) stress, we investigated and analyzed GRP78 expression levels in the trabecular meshwork (TM) by eyes with primary open angle glaucoma (POAG) and normal eyes to understand the role of GRP78 in human TM cells apoptosi...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Chai F,Luo R,Li Y,Bai Y,He Y,Wei Y,Yan Z,Ge J,Zhuo Y

    更新日期:2010-06-17 00:00:00

  • Polymorphisms in the VEGFA and VEGFR-2 genes and neovascular age-related macular degeneration.

    abstract:PURPOSE:Genetic factors influence an individual's risk for developing neovascular age-related macular degeneration (AMD), a leading cause of irreversible blindness. Previous studies on the potential genetic link between AMD and vascular endothelial growth factor (VEGF), a key regulator of angiogenesis and vascular perm...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Fang AM,Lee AY,Kulkarni M,Osborn MP,Brantley MA Jr

    更新日期:2009-12-10 00:00:00

  • Vitreous induces heme oxygenase-1 expression mediated by transforming growth factor-beta and reactive oxygen species generation in human retinal pigment epithelial cells.

    abstract:PURPOSE:When human retinal pigment epithelial (RPE) cells come in contact with vitreous, they undergo changes in gene expression that include inflammatory and anti-oxidant responses. The effects of vitreous on expression of heme oxygenase-1 (HO-1), metallothionein (MT) -1a and -2a, and c-fos were investigated. Activato...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Hartung R,Parapuram SK,Ganti R,Hunt DM,Chalam KV,Hunt RC

    更新日期:2007-01-24 00:00:00

  • Polymorphisms of chemokine and chemokine receptor genes in idiopathic immune-mediated posterior segment uveitis.

    abstract:PURPOSE:Chemokines are important inflammatory mediators that play a crucial role in uveitis. Polymorphisms in chemokine genes can alter the expression of these genes in the inflammatory cells, which, in turn, can affect the clinical phenotype of the disease. The purpose of this study was to identify polymorphisms in ch...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Ahad MA,Missotten T,Abdallah A,Lympany PA,Lightman S

    更新日期:2007-03-23 00:00:00

  • Exon screening of the genes encoding the beta- and gamma-subunits of cone transducin in patients with inherited retinal disease.

    abstract:PURPOSE:To screen the exons of the genes encoding the beta3-subunit (GNB3) and gammac-subunit (GNGT2) of cone transducin for mutations in a large number of unrelated patients with various forms of inherited retinal disease including cone dystrophy, cone-rod dystrophy and macular dystrophy. METHODS:Exons of the two gen...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Gao YQ,Danciger M,Akhmedov NB,Zhao DY,Heckenlively JR,Fishman GA,Weleber RG,Jacobson SG,Farber DB

    更新日期:1998-09-17 00:00:00

  • Fine mapping of the usher syndrome type IC to chromosome 11p14 and identification of flanking markers by haplotype analysis.

    abstract:PURPOSE:To refine the map position of the Usher syndrome type 1C (USH1C) locus to 11p14-p15.1 in the French-Acadian population settled in Louisiana. METHODS:Linkage and haplotype analysis of Ush1C in the French-Acadian families from southwestern Louisiana was carried out using additional markers known to map to the US...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Ayyagari R,Li Y,Smith RJ,Pelias MZ,Hejtmancik JF

    更新日期:1995-10-25 00:00:00

  • Fasudil inhibits LPS-induced migration of retinal microglial cells via regulating p38-MAPK signaling pathway.

    abstract:PURPOSE:To investigate the effect and possible molecular mechanisms of fasudil on retinal microglial (RMG) cell migration. METHODS:Primary cultured RMG cells were incubated with lipopolysaccharide (LPS), fasudil, and/or SB203580 (a p38 inhibitor). RMG cell motility was determined with the scratch wound assay and the T...

    journal_title:Molecular vision

    pub_type: 杂志文章

    doi:

    authors: Xu F,Xu Y,Zhu L,Rao P,Wen J,Sang Y,Shang F,Liu Y

    更新日期:2016-07-19 00:00:00