Abstract：
PURPOSE:The purpose of this study was to reassess the role of the lens as an "embryonic organizer" of ocular tissues. METHODS:We ablated the lens in mice by lens-specific expression of an attenuated version of diphtheria toxin A subunit(Tox176) driven by a modified crystallin promoter. Alterations in the differentiation programs of ocular tissues were examined by hematoxylin and eosin staining, in situ hybridization, and immunohistochemistry. RESULTS:Transgenic mice in the family OVE1757 exhibited severe microphakia. Apoptotic lens fibers were seen by embryonic day 15 (E15) and the lenses were completely ablated by post natal day 8. Multiple defects were seen in the anterior chamber. Corneal endothelial cells did not differentiate properly. The mesenchymal cells that would normally give rise to the endothelial layer were found to express N-cadherin, but they failed to form tight junctions and undergo a mesenchymal-to-epithelial transition. Although early specification of the presumptive ciliary body and iris was detected, subsequent differentiation of the iris was blocked. No dramatic changes were seen in the development of the retina. CONCLUSIONS:These results support the hypothesis that an intact lens is essential for proper differentiation of both the corneal endothelium and the iris and that the lens "organizes" the development of tissues in the anterior chamber.
journal_name
Mol Visjournal_title
Molecular visionauthors
Zhang Y,Overbeek PA,Govindarajan Vsubject
Has Abstractpub_date
2007-12-18 00:00:00pages
2289-300issn
1090-0535pii
v13/a259journal_volume
13pub_type
杂志文章相关文献
MOLECULAR VISION文献大全abstract：PURPOSE:Pathologic angiogenesis in the retina leads to the catastrophic loss of vision. Retinopathy of prematurity (ROP), a vasoproliferative retinopathy, is a leading cause of blindness in children. We evaluated the inhibitory effect of decursin on retinal neovascularization. METHODS:Anti-angiogenic activity of decur...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2009-09-12 00:00:00
abstract：PURPOSE:Increased superoxide levels are implicated in the pathogenesis of diabetic retinopathy. We have shown that functional activation of a small molecular weight G-protein, H-Ras, is one of the signaling steps involved in glucose-induced apoptosis of retinal capillary cells. The goal of this study was to elucidate t...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2007-04-19 00:00:00
abstract：PURPOSE:Human corneal endothelium exhibits both age-related and topographical differences in relative proliferative capacity and in senescence characteristics. The purpose of these studies was to compare telomere lengths in human corneal endothelial cells (HCEC) from the central and peripheral areas of corneas from you...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2007-07-23 00:00:00
abstract：PURPOSE:To identify the cause of congenital cataracts in a consanguineous family of Ashkenazi Jewish ancestry. METHODS:We performed genome-wide linkage analysis and whole-exome sequencing for the initial discovery of variants, and we confirmed the variants using gene-specific primers and Sanger sequencing. RESULTS:We...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2014-11-06 00:00:00
abstract：PURPOSE:The dopamine (DA) system in the retina is critical to normal visual development as lack of retinal DA signaling may contribute to myopic development. The involvement of DA in myopic development is complex and may be different between form deprivation and hyperopic defocus. This study evaluated effects of a non-...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
abstract：PURPOSE:To investigate the role of multigenic variation in primary open-angle glaucoma (POAG) involving the rRNA processing gene WD repeat domain 36 (WDR36). METHODS:We examined the heat shock protein 70/90 (HSP70/90)-organizing co-chaperone stress-induced-phosphoprotein 1 (STI1) as a potential co-modifying gene in gl...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
abstract：PURPOSE:The aim of the present study was to investigate the molecular basis underlying a nonsyndromic presenile autosomal dominant cataract in a three-generation pedigree. The phenotype was progressive from a peripheral ring-like opacity to a total cataract with advancing age from teenage to adulthood. The visual impai...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2006-07-12 00:00:00
abstract：PURPOSE:Diabetic retinopathy (DR) is one of the most important microvascular complications in both type 1 and type 2 diabetes. In Brazil, its proliferative form is the second cause of irreversible blindness among adults of working age. Despite the strong association of DR with disease duration and degree of chronic hyp...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2007-05-23 00:00:00
abstract：PURPOSE:Autosomal dominant early-onset long anterior zonules (LAZs) and late-onset retinal degeneration (L-ORD) in humans are associated with the S163R mutation of the complement 1q-tumor necrosis factor related protein-5 (CTRP5) gene. For using the pig as an L-ORD model for the study of pathology, we cloned, character...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：PURPOSE:KH902 is a fusion protein which combines ligand binding elements taken from the extracellular domains of vascular endothelial growth factor (VEGF) receptors 1 and 2 and the Fc portion of IgG1. This study is designed to examine the inhibitory effect of KH902 in the choroidal neovascularization (CNV) monkey model...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2008-01-10 00:00:00
abstract：PURPOSE:To investigate the effect of intracameral injection of fibrin tissue sealant on the anterior segment structures in a rabbit model. METHODS:One eye of 10 rabbits received an intracameral injection of fibrin tissue sealant with a thrombin concentration of 500 IU (TISSEEL), and the fellow eye received an intracam...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2010-06-12 00:00:00
abstract：PURPOSE:Controversy exists over melanogenesis of adult human RPE cells in vitro. This study investigated melanin content and production and expression of tyrosinase (TYR), tyrosinase-related-protein-1 (TRP1), tyrosinase-related-protein-2 (TRP2), and P gene in cultured human RPE cells and uveal melanoma cells. METHODS:...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2007-11-03 00:00:00
abstract：PURPOSE:alpha-Crystallin is the major protein of the mammalian lens where it contributes to the refractive properties needed for vision and possibly to the stability of the tissue. The aim of this study was to determine whether the properties of alpha-crystallin have changed during the course of evolution. METHODS:Dog...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2009-11-20 00:00:00
abstract：PURPOSE:Waardenburg syndrome (WS) is characterized by sensorineural hearing loss and pigmentation defects of the eye, skin, and hair. It is caused by mutations in one of the following genes: PAX3 (paired box 3), MITF (microphthalmia-associated transcription factor), EDNRB (endothelin receptor type B), EDN3 (endothelin ...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：PURPOSE:Cone transducin plays an important role in interacting with the cone photoreceptor visual pigments and activating the cGMP-dependent phosphodiesterase. The human gene for the alpha-subunit of cone transducin (GNAT2) has been cloned and characterized. Recently achromatopsia has been associated with mutations in ...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2004-04-08 00:00:00
abstract：PURPOSE:In numerous clinical and experimental studies, preservatives present in eye drops have had detrimental effects on ocular epithelial cells. The aim of this study was to compare the cytotoxic and inflammatory effects of the preservative polyquaternium-1 (PQ-1) containing Travatan (travoprost 0.004%) and Systane U...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：PURPOSE:To determine the contribution of conjunctival mast cells to the allergen-specific inflammatory responses in eyes with allergic conjunctivitis and to test the hypothesis that mast cells act as mediators of the early phase response. METHODS:The participation of mast cells in allergen-induced inflammatory cell re...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2008-08-22 00:00:00
abstract：PURPOSE:We previously demonstrated that tenascin-C was highly expressed in the fibrovascular membranes (FVMs) of patients with proliferative diabetic retinopathy (PDR). However, its role in the pathogenesis of FVMs has not been determined. The purpose of this study was to investigate what role tenascin-C plays in the f...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2016-04-30 00:00:00
abstract：PURPOSE:To investigate whether apoptosis of human lens epithelial cells (HLECs) can be induced with the polyamidoamine (PAMAM)-mediated inhibition of bcl-2 (b-cell lymphoma 2) by small hairpin RNA (shRNA). METHODS:HLECs (SRA01/04) were transfected with the fifth generation of PAMAM (PAMAM G5) by bcl-2 shRNA. At 24, 48...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：PURPOSE:Genetic factors influence an individual's risk for developing neovascular age-related macular degeneration (AMD), a leading cause of irreversible blindness. Previous studies on the potential genetic link between AMD and vascular endothelial growth factor (VEGF), a key regulator of angiogenesis and vascular perm...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2009-12-10 00:00:00
abstract：PURPOSE:To characterize the disease-causing mutations and related phenotypes in two Chinese families with autosomal dominant congenital cataract. METHODS:Family members were clinically characterized by a complete eye examination. Genome-wide linkage screening was performed in Family 1 using a 10K single nucleotide pol...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2007-07-13 00:00:00
abstract：PURPOSE:The purpose of the current study was to investigate the effect of topical administration of KH906 on corneal neovascularization (NV). METHODS:To induce corneal neovascularization, chemical cauterization of the corneas of the right eyes of forty-eight New Zealand white rabbits was performed by touching the cent...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2011-03-25 00:00:00
abstract：INTRODUCTION:Elevated intraocular pressure (IOP) in glaucomatous eyes is often due to increased resistance to aqueous outflow. Previous studies have shown that increased extracellular material deposition in outflow pathways leads to increased resistance to aqueous outflow, and transforming growth factor (TGF)-β seems t...
journal_title：Molecular vision
pub_type： 杂志文章,meta分析
doi：
更新日期：2015-05-25 00:00:00
abstract：PURPOSE:To examine the characteristics of retinal pigment epithelial (RPE) cells cultured on amniotic membrane (AM). The present study examined how AM modulates RPE cell differentiation. METHODS:Human RPE cells were cultured on the basement membrane side of dispase treated AM. After one week of cellular confluence, cu...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2005-01-06 00:00:00
abstract：PURPOSE:To determine the age-related and the cataract-specific changes in the crystallin composition in lenses of accelerated-senescence OXYS (cataract model) and Wistar (control) rats. METHODS:The water soluble (WS) and insoluble (WIS) fractions of the lens proteins were separated; the identity and relative abundance...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
abstract：PURPOSE:To establish an untransfected human corneal endothelial (HCE) cell line and characterize its biocompatibility to denuded amniotic membrane (dAM). METHODS:Primary culture was initiated with a pure population of HCE cells in DMEM/F12 media (pH 7.2) containing 20% fetal bovine serum and various supplements. The e...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2011-02-15 00:00:00
abstract：PURPOSE:The retina plays an important regulatory role in ocular growth. To screen for new retinal candidate genes that could be involved in the inhibition of ocular growth, we used chick microarrays to analyze the changes in retinal mRNA expression after myopic defocus was imposed by positive lens wear. METHODS:Four m...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2008-08-31 00:00:00
abstract：Purpose:Blue cone monochromacy (BCM) is an X-linked congenital vision disorder characterized by complete loss or severely reduced L- and M-cone function. Patients with BCM display poor visual acuity, severely impaired color discrimination, myopia, nystagmus, and minimally detectable cone-mediated electroretinogram. Rec...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2018-01-08 00:00:00
abstract：PURPOSE:To evaluate whether transfection of human retinal endothelial cells (HRECs) with plasmids expressing ribozymes designed to specifically cleave the mRNA and reduce expression of either vascular endothelial growth factor (VEGF) receptor-1 (VEGFR-1), or VEGF receptor-2 (VEGFR-2), or insulin-like growth factor-I re...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2006-01-12 00:00:00
abstract：PURPOSE:To establish and characterize a novel untransfected corneal endothelial cell line from New Zealand white rabbits (NRCE cell line) for studies on corneal endothelial cells. METHODS:Primary culture was initiated with a pure population of NRCE cells from corneal endothelia by successive detachment and reattachmen...
journal_title：Molecular vision
pub_type： 杂志文章
doi：
更新日期：2009-05-29 00:00:00
