Abstract:
:Motor endplates (MEPs) are important sites of information exchange between motor neurons and skeletal muscle, and are distributed in an organized pattern of lamellae in the muscle. Delayed repair of peripheral nerve injury typically results in unsatisfactory functional recovery because of MEP degeneration. In this study, the mouse tibial nerve was transected and repaired with a biodegradable chitin conduit, immediately following or 1 or 3 months after the injury. Fluorescent α-bungarotoxin was injected to label MEPs. Tissue optical clearing combined with light-sheet microscopy revealed that MEPs were distributed in an organized pattern of lamellae in skeletal muscle after delayed repair for 1 and 3 months. However, the total number of MEPs, the number of MEPs per lamellar cluster, and the maturation of single MEPs in gastrocnemius muscle gradually decreased with increasing denervation time. These findings suggest that delayed repair can restore the spatial distribution of MEPs, but it has an adverse effect on the homogeneity of MEPs in the lamellar clusters and the total number of MEPs in the target muscle. The study procedures were approved by the Animal Ethics Committee of the Peking University People's Hospital (approval No. 2019PHC015) on April 8, 2019.
journal_name
Neural Regen Resjournal_title
Neural regeneration researchauthors
Li DD,Deng J,Jin B,Han S,Gu XY,Zhou XF,Yin XFdoi
10.4103/1673-5374.317990keywords:
["degeneration","delayed repair","lamellar cluster","light-sheet microscopy","motor endplates","peripheral nerve injury","three-dimensional distribution","tissue optical clearing\n"]subject
Has Abstractpub_date
2022-02-01 00:00:00pages
459-464issue
2eissn
1673-5374issn
1876-7958pii
NeuralRegenRes_2022_17_2_459_317990journal_volume
17pub_type
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