Abstract:
:We used the standard comet assay successfully to generate in vitro dose-response curves under oxic and hypoxic conditions. We then made mixtures of cells that had been irradiated with 3 and 9 Gy of X-rays to simulate two subpopulations in a tumor, but efforts to accurately detect and quantify the subpopulations using the standard comet assay were unsuccessful. Therefore, we investigated a modified comet assay to determine whether it could be used for measuring hypoxia in our model systems. U251 MG cells were grown as subcutaneous tumors in athymic mice; U251 MG and U87 MG cells were grown as intracerebral (i.c.) tumors in athymic rats. Animals were injected with RSU 1069, irradiated, and euthanized. Tumors and normal brains were removed, and the cells were analyzed using a modified comet assay. Differences in comet tail moment distributions between tumor and contralateral normal brain, using tail moments at either the 25th or 50th percentile in each distribution, were taken as measures of the degree of tumor hypoxia. For U251 MG tumors, there was a positive relationship between tumor size and the degree of hypoxia, whereas preliminary data from U87 MG i.c. tumors showed less hypoxia and no apparent relationship between tumor size and hypoxia.
journal_name
Neoplasiajournal_title
Neoplasia (New York, N.Y.)authors
Wang J,Klem J,Wyrick JB,Ozawa T,Cunningham E,Golinveaux J,Allen MJ,Lamborn KR,Deen DFdoi
10.1016/S1476-5586(03)80022-2keywords:
subject
Has Abstractpub_date
2003-07-01 00:00:00pages
288-96issue
4eissn
1522-8002issn
1476-5586pii
S1476-5586(03)80022-2journal_volume
5pub_type
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