Detection of CD44 variants in fine needle aspiration biopsies of thyroid tumor by RT-PCR.

Abstract:

:Increased expression of CD44 variants is often observed in differentiated thyroid carcinomas. To establish a method for gene diagnosis of thyroid without any additional invasions to the patients, RNA in leftover cells inside the needle used for fine needle aspiration biopsies (FNABs) was extracted to detect the expression of CD44 variants by reverse transcription-polymerase chain reaction (RT-PCR). After FNAB and preparation of sample on a slide glass for cytological examinations, RNA in leftover cells inside the needle was extracted. After reverse transcription, cDNA was amplified by PCR with specific primers for CD44. The results were compared with the cytological diagnosis. CD44 variants were detectable in all seven samples that were diagnosed as positive cytology. However, they were detected in only five of the 29 samples of negative cytology. Therefore, thyroid tumors may be more accurately diagnosed by a combination of cytological examination and detection of CD44 variants in RNA obtained by FNAB.

journal_name

J Exp Clin Cancer Res

authors

Takano T,Sumizaki H,Amino N

subject

Has Abstract

pub_date

1997-09-01 00:00:00

pages

267-71

issue

3

eissn

0392-9078

issn

1756-9966

journal_volume

16

pub_type

杂志文章
  • NF-κB maintains the stemness of colon cancer cells by downregulating miR-195-5p/497-5p and upregulating MCM2.

    abstract:BACKGROUND:Colon cancer represents one of the leading causes of gastrointestinal tumors in industrialized countries, and its incidence appears to be increasing at an alarming rate. Accumulating evidence has unveiled the contributory roles of cancer stem cells (CSCs) in tumorigenicity, recurrence, and metastases. The fu...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-020-01704-w

    authors: Wang L,Guo J,Zhou J,Wang D,Kang X,Zhou L

    更新日期:2020-10-28 00:00:00

  • Relationship between human mammaglobin mRNA expression in breast cancer tissue and clinico-pathologic features of the tumors.

    abstract::Human mammaglobin (hMAM) has recently been recognized as a breast associated glycoprotein. Although the biological role of hMAM is unknown, it has been previously reported that hMAM gene expression is a marker of low biological and clinical aggressiveness of breast cancer (BC). In this study, 148 cases of BC tissues w...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Roncella S,Ferro P,Bacigalupo B,Dessanti P,Giannico A,Gorji N,Moroni M,Tozzini S,Pensa F,Gianquinto D,Fais F,Pronzato P,Fedeli F

    更新日期:2006-03-01 00:00:00

  • miR-539 inhibits prostate cancer progression by directly targeting SPAG5.

    abstract:BACKGROUND:We conducted multiple microarray datasets analyses from clinical and xenograft tumor tissues to search for disease progression-driving oncogenes in prostate cancer (PCa). Sperm-associated antigen 5 (SPAG5) attracted our attention. SPAG5 was recently identified as an oncogene participating in lung cancer and ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-016-0337-8

    authors: Zhang H,Li S,Yang X,Qiao B,Zhang Z,Xu Y

    更新日期:2016-04-01 00:00:00

  • Viruses in human oral cancers.

    abstract::The role of viruses in the etiology of human oral cancer is critically reviewed. Available evidences show a positive correlation for human oral cancer with human papilloma virus (HPV), herpes simplex virus (HSV) and human herpes virus type-6 (HHV-6), having strongest association with HPV. These viruses may act alone o...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:

    authors: Vasudevan DM,Vijayakumar T

    更新日期:1998-03-01 00:00:00

  • Oncogene addiction in gliomas: implications for molecular targeted therapy.

    abstract::Oncogene addiction is a phenomenon that the survival of cancer cells depends on an activated oncogene or inactivation of tumor suppressor gene, and is regarded as the 'Achilles heel' of the successful molecular targeted therapies in cancer. However, the role of oncogene addiction in gliomas has not been elucidated sys...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/1756-9966-30-58

    authors: Yan W,Zhang W,Jiang T

    更新日期:2011-05-17 00:00:00

  • Gut-endocrinomas (carcinoids and related endocrine variants) of the uterine cervix: an analysis of 205 reported cases.

    abstract::This study was carried out statistically to evaluate the present situation of carcinoids and related endocrine variants in the uterine cervix. A total of 205 patients who had been reported on in world-wide literature were divided into two groups; one, as a carcinoid group, consisting of 81 patients with carcinoids, 49...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Soga J,Osaka M,Yakuwa Y

    更新日期:2001-09-01 00:00:00

  • Palliative treatment of painful bone metastases from non-Hodgkin lymphoma with disodium pamidronate.

    abstract::We report a case of a 72-year-old male, with a known history of non-Hodgkin lymphoma of the left tonsil for two years, histologically proved and successfully treated by radical surgical excision in combination with external radiotherapy. He presented with diffuse bone pain the last month, especially at the lower left ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Pistevou-Gombaki K,Eleftheriadis N,Sofroniadis I,Makris P,Kouloulias V

    更新日期:2002-09-01 00:00:00

  • Loss of expression of Kruppel-like factor 6 in primary hepatocellular carcinoma and hepatoma cell lines.

    abstract::KLF6 (Zf9, COPEB), an ubiquitous transcription factor, maps to chromosome 10p. Recently, KLF6 was found to have a more generalized role in tumorigenesis as a candidate tumor suppressor gene for some tumors. However, results from other published studies seem not to be in agreement with data from previous studies. Gene-...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Wang SP,Zhou HJ,Chen XP,Ren GY,Ruan XX,Zhang Y,Zhang RL,Chen J

    更新日期:2007-03-01 00:00:00

  • Human mammaglobin mRNA is a reliable molecular marker for detecting occult breast cancer cells in peripheral blood.

    abstract::Occult carcinoma cells in peripheral blood of breast cancer (BC) patients is generally associated with poor disease prognosis. Reverse transcriptase-polymerase chain reaction (RT-PCR) is a sensitive method for revealing rare circulating cancer cells. The target mRNA must be carefully chosen, as it must be expressed on...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Roncella S,Ferro P,Bacigalupo B,Pronzato P,Tognoni A,Falco E,Gianquinto D,Ansaldo V,Dessanti P,Fais F,Rosai J,Fedeli F

    更新日期:2005-06-01 00:00:00

  • microRNA-96 promotes occurrence and progression of colorectal cancer via regulation of the AMPKα2-FTO-m6A/MYC axis.

    abstract:BACKGROUND:Colorectal cancer (CRC) is one of the frequently occurred malignancies in the world. To date, several onco-microRNAs (miRNAs or miRs), including miR-96, have been identified in the pathogenesis of CRC. In the present study, we aimed to corroborate the oncogenic effect of miR-96 on CRC and to identify the spe...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-020-01731-7

    authors: Yue C,Chen J,Li Z,Li L,Chen J,Guo Y

    更新日期:2020-11-12 00:00:00

  • Evaluation of naphthalmustine, a nitrogen mustard derivative of naphthalimide as a rationally-designed anticancer agent.

    abstract::Naphthalmustine, 2-[2-[bis-(2-chloroethyl)amino]ethyl]-1H-benz[de]isoquinoline-1,3-dione (Compound 1) has been synthesized as a rationally designed new anticancer agent from N-(2-bromoethyl)naphthalimide. Its chemical alkylating activity exceeded that of nor-HN2 used as standard compound for comparison. Its antitumour...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Pain A,Samanta S,Dutta S,Saxena AK,Shanmugavel M,Kampasi H,Qazi GN,Sanyal U

    更新日期:2003-09-01 00:00:00

  • From bench to bed: the tumor immune microenvironment and current immunotherapeutic strategies for hepatocellular carcinoma.

    abstract::Hepatocellular carcinoma (HCC) ranks the most common primary liver malignancy and the third leading cause of tumor-related mortality worldwide. Unfortunately, despite advances in HCC treatment, less than 40% of HCC patients are eligible for potentially curative therapies. Recently, cancer immunotherapy has emerged as ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/s13046-019-1396-4

    authors: Fu Y,Liu S,Zeng S,Shen H

    更新日期:2019-09-09 00:00:00

  • A multicenter prospective phase II randomized trial of epirubicin/vinorelbine versus pegylated liposomal doxorubicin/vinorelbine as first-line treatment in advanced breast cancer. A GOIM study.

    abstract:BACKGROUND:To evaluate activity and tolerability of two anthracycline-containing regimens as first-line treatment for anthracycline-naïve relapsed breast cancer patients. METHODS:Patients with relapsed breast cancer not previously treated with adjuvant anthracyclines were randomly assigned to epirubicin/vinorelbine (a...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1186/1756-9966-30-39

    authors: Vici P,Colucci G,Giotta F,Sergi D,Filippelli G,Perri P,Botti C,Vizza E,Carpino A,Pizzuti L,Latorre A,Giannarelli D,Lopez M,Di Lauro L

    更新日期:2011-04-12 00:00:00

  • Upregulation of DARS2 by HBV promotes hepatocarcinogenesis through the miR-30e-5p/MAPK/NFAT5 pathway.

    abstract:BACKGROUND:Infection with the hepatitis B virus (HBV) is closely associated with the development of hepatocellular carcinoma (HCC). The osmoregulatory transcription factor nuclear factor of activated T-cells 5 (NFAT5) has been shown to play an important role in the development of many types of human cancers. The role o...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-017-0618-x

    authors: Qin X,Li C,Guo T,Chen J,Wang HT,Wang YT,Xiao YS,Li J,Liu P,Liu ZS,Liu QY

    更新日期:2017-10-19 00:00:00

  • Transfection of mammalian cells by the methods of receptor mediated gene transfer and particle bombardment.

    abstract::The efficacy of currently developed methods for gene transfer into mammalian cells depends primarily on the transfection technique, and also on the type of targeted cells. Considering the importance of gene transfer in the creation of gene therapies, our study was aimed at the assessment of transfection capacity of re...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Novaković S,Knezević M,Golouh R,Jezersek B

    更新日期:1999-12-01 00:00:00

  • p38α MAPK-mediated induction and interaction of FOXO3a and p53 contribute to the inhibited-growth and induced-apoptosis of human lung adenocarcinoma cells by berberine.

    abstract:BACKGROUND:Berberine (BBR), a component from traditional Chinese medicine, has been shown to possess anti-tumor activity against a wide spectrum of cancer cells including human lung cancer, but the detailed mechanism underlining this has not been well elucidated. METHODS:In this study, the effect of berberine on cell ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-33-36

    authors: Zheng F,Tang Q,Wu J,Zhao S,Liang Z,Li L,Wu W,Hann S

    更新日期:2014-04-26 00:00:00

  • Evaluation of HER2 and p53 expression in predicting response to docetaxel-based first-line chemotherapy in advanced breast cancer.

    abstract:BACKGROUND:The human epidermal growth factor receptor 2 (HER2) and p53 pathways may be involved in chemotherapy sensitivity and/or resistance. We explore the value of HER2 and p53 status to foretell docetaxel sensitivity in advanced breast cancer. METHODS:HER2 and p53 expression was analysed in 36 (median age 55 yrs; ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-30-38

    authors: Camerini A,Donati S,Viacava P,Siclari O,Puccetti C,Tartarelli G,Valsuani C,De Luca F,Martini L,Cavazzana A,Amoroso D

    更新日期:2011-04-11 00:00:00

  • Receptor tyrosine kinase inhibitor Sunitinib and integrin antagonist peptide HM-3 show similar lipid raft dependent biphasic regulation of tumor angiogenesis and metastasis.

    abstract:BACKGROUND:Anti-angiogenesis remains an attractive strategy for cancer therapy. Some anti-angiogenic reagents have bell-shape dose-response curves with higher than the effective doses yielding lower anti-angiogenic effects. In this study, two different types of anti-angiogenic reagents, a receptor tyrosine kinase inhib...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,收录出版

    doi:10.1186/s13046-019-1324-7

    authors: Hu J,Wang W,Liu C,Li M,Nice E,Xu H

    更新日期:2019-08-28 00:00:00

  • AIDS-Kaposi Sarcoma and Classic Kaposi Sarcoma: are different ultrasound patterns related to different variants?

    abstract:BACKGROUND:Kaposi Sarcoma (KS) is a malignancy of endothelial skin cells with multifocal localization on the skin, lymph nodes and visceral organs. Although all clinical variants are associated with HHV-8 infection, specific differences in the clinical onset and in the natural history of AIDS-KS and Classic-KS have bee...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-30-40

    authors: Solivetti FM,Elia F,Latini A,Cota C,Cordiali-Fei P,Di Carlo A

    更新日期:2011-04-13 00:00:00

  • Breast cancer susceptibility gene 1 (BRCA1) predict clinical outcome in platinum- and toxal-based chemotherapy in non-small-cell lung cancer (NSCLC) patients: a system review and meta-analysis.

    abstract::The recent studies have evaluated the relationship between BRCA1 expression and clinical outcome of chemotherapy (mainly focused on platinum-based and toxal-based treatment) in NSCLC patients, but the results were inconclusive and controversial. Our aim of this study was to evaluate this association by literature base...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,meta分析,评审

    doi:10.1186/1756-9966-32-15

    authors: Yang Y,Xie Y,Xian L

    更新日期:2013-03-15 00:00:00

  • Formononetin inhibits tumor growth by suppression of EGFR-Akt-Mcl-1 axis in non-small cell lung cancer.

    abstract:BACKGROUND:Epidermal growth factor receptor (EGFR) activating mutations play crucial roles in the tumorigenesis of human non-small cell lung cancer (NSCLC). The mechanism regarding how EGFR signaling regulates myeloid cell leukemia sequence 1 (Mcl-1) protein stability and ubiquitination remains undefined. METHODS:MTS ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-020-01566-2

    authors: Yu X,Gao F,Li W,Zhou L,Liu W,Li M

    更新日期:2020-04-10 00:00:00

  • Cell cycle proteins in laryngeal cancer: role in proliferation and prognosis.

    abstract::A study of laryngeal carcinomas was performed in order to analyze (a) the expression of p53/p21, cyclin D1/cyclin E, p21/p27 (b) the relation of normal and abnormal protein expression, with the proliferation status, as determined by the expression of Ki67 and PCNA and (c) the correlation of our findings with prognosis...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Peschos D,Stefanou D,Vougiouklakis T,Assimakopoulos DA,Agnantis NJ

    更新日期:2005-09-01 00:00:00

  • Papillary renal cell carcinoma presenting as nodal metastases to the neck.

    abstract::Renal cell carcinoma, in a high percentage of patients, metastasizes early, sometimes mimicking other lesions. We present a case of an asymptomatic papillary renal cell carcinoma that presented neck metastases as the initial manifestation. The laterocervical and supraclavicular masses were considered consistent with n...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Giuliani A,Caporale A,Borghese M,Galati G,Di Bari M,Demoro M

    更新日期:1999-12-01 00:00:00

  • Role of retinoic receptors in lung carcinogenesis.

    abstract::Several in vitro and in vivo studies have examined the positive and negative effects of retinoids (vitamin A analogs) in premalignant and malignant lesions. Retinoids have been used as chemopreventive and anticancer agents because of their pleiotropic regulator function in cell differentiation, growth, proliferation a...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/1756-9966-27-18

    authors: Bogos K,Renyi-Vamos F,Kovacs G,Tovari J,Dome B

    更新日期:2008-07-14 00:00:00

  • sE-cadherin serves as a diagnostic and predictive parameter in prostate cancer patients.

    abstract:BACKGROUND:Measurement of prostate-specific antigen (PSA) advanced the diagnostic and prognostic potential for prostate cancer (PCa). However, due to PSA's lack of specificity, novel biomarkers are needed to improve risk assessment and ensure optimal personalized therapy. A set of protein molecules as potential biomark...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-015-0161-6

    authors: Tsaur I,Thurn K,Juengel E,Gust KM,Borgmann H,Mager R,Bartsch G,Oppermann E,Ackermann H,Nelson K,Haferkamp A,Blaheta RA

    更新日期:2015-05-14 00:00:00

  • Radionuclide therapy with bone seeking radionuclides in palliation of painful bone metastases.

    abstract::Radionuclide therapy with bone seeking radiopharmaceuticals is one of the oldest interventions in nuclear medicine and has been now in use for many years as an effective method of palliating painful bone metastases. Clinical indications have slowly but progressively increased starting from end-stage prostate cancer wi...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:

    authors: Maini CL,Sciuto R,Romano L,Bergomi S

    更新日期:2003-12-01 00:00:00

  • Cisplatin triggers cancer stem cell enrichment in platinum-resistant cells through NF-κB-TNFα-PIK3CA loop.

    abstract:BACKGROUND:Parallel to complex alteration in molecular and cellular events, enrichment of cancer stem cells (CSC) contributes significantly in deliberation and maintenance of cisplatin resistance. Cisplatin mediated CSC enrichment is well established in various cancers, yet the underlying mechanism is largely unknown. ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-017-0636-8

    authors: Thakur B,Ray P

    更新日期:2017-11-23 00:00:00

  • Efficacy and adverse effects of transdermal fentanyl and sustained-release oral morphine in treating moderate-severe cancer pain in Chinese population: a systematic review and meta-analysis.

    abstract:BACKGROUND:Previous meta-analysis suggested that transdermal fentanyl was not inferior to sustained-release oral morphine in treating moderate-severe cancer pain with less adverse effects. Now, we updated the data and performed a systematic review. METHODS:Updated cohort studies on transdermal fentanyl and oral morphi...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,meta分析

    doi:10.1186/1756-9966-29-67

    authors: Yang Q,Xie DR,Jiang ZM,Ma W,Zhang YD,Bi ZF,Chen DL

    更新日期:2010-06-09 00:00:00

  • Combined effect of arsenic trioxide and sulindac sulfide in A549 human lung cancer cells in vitro.

    abstract::Arsenic trioxide has shown substantial efficacy in treating patients with relapsed or refractory acute promyelocytic leukemia (APL) as well as solid tumors. Arsenic can act through a considerable number of different pathways including mitochondrial respiration and tubulin formation, affecting growth, blood flow, diffe...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Jiang TT,Brown SL,Kim JH

    更新日期:2004-06-01 00:00:00

  • Correction to: Liquid biopsy and tumor heterogeneity in metastatic solid tumors: the potentiality of blood samples.

    abstract::An amendment to this paper has been published and can be accessed via the original article. ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,已发布勘误

    doi:10.1186/s13046-020-01615-w

    authors: Russano M,Napolitano A,Ribelli G,Iuliani M,Simonetti S,Citarella F,Pantano F,Dell'Aquila E,Anesi C,Silvestris N,Argentiero A,Solimando AG,Vincenzi B,Tonini G,Santini D

    更新日期:2020-06-24 00:00:00