当前位置： SCI文献检索 > JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION期刊下所有文献 > Usefulness of a chromatographic method to detect circulating antithyroid hormone autoantibodies in canine serum.

Usefulness of a chromatographic method to detect circulating antithyroid hormone autoantibodies in canine serum.

Abstract：

:Antithyroid hormone autoantibodies (THBA), described in both humans and animals, result in variable interference when thyroid hormone plasma levels are measured by immunoassays. We previously described a quick chromatographic method to detect circulating THBA in humans. In the present experience, we applied the method on canine sera (10 normal dogs and 3 dogs affected by hypothyroidism) to detect the THBA presence in a dog (no. 13) with clinical evidence of hypothyroidism, in spite of apparently extremely high values of thyroid hormone. After a short incubation of samples with 125I-T3 and 125I-T4 in presence of 8-anilino-1-naphtalenesulfonic acid, samples were eluted and radioactivity values counted. Eluate radioactivity values > 10% and > 30% were considered positive for THBA presence for antiT3 and antiT4 detection, respectively. High radioactivity values were detected in dog serum no. 13 and, therefore, it was considered positive for THBA presence. The Scatchard plot analysis revealed the presence of a monoclonal autoantibody with the highest affinity for T3 and an additional tenfold lower affinity for T4. In conclusion, our chromatographic method allows the detection and the characterization of THBA in species different from humans, with species specific differences in thyroid hormone metabolism; thus, taking into account the rarely availability of canine serum TSH and anti-thyroglobulin antibody immunoassay detection methods, it was possible to correctly diagnose the hypothyroidism in a dog with apparently extremely high values of thyroid hormones due to THBA interference.

journal_name

J Endocrinol Invest

journal_title

Journal of endocrinological investigation

authors

Savastano S,Tommaselli AP,Valentino R,Dorato M,Scarpitta MT,Persechino A,Ciaramella P,Lombardi G

doi

10.1007/BF03347880

subject

Has Abstract

pub_date

1996-12-01 00:00:00

pages

758-62

issue

11

eissn

0391-4097

issn

1720-8386

journal_volume

19

pub_type

杂志文章

相关文献

JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION文献大全
  • Assessment of non-traumatic vertebral fractures in Cushing's syndrome patients.

    abstract：PURPOSE:Hypercortisolism has detrimental effects on bone metabolism with the consequences of bone loss and bone fractures. We aimed to evaluate the frequency of vertebral fragility fractures and to determine the factors associated with Cushing's syndrome (CS). METHODS:A total of 135 patients diagnosed with Cushing's s...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/s40618-020-01496-y

    authors： Apaydın T,Yavuz DG

    更新日期：2021-01-09 00:00:00

  • P53 mutations in thyroid carcinoma: tidings from an old foe.

    abstract：:The underlying mechanism leading to carcinogenesis involves genomic instability, likely related to aneuploidy. While p53 as a "guardian of the genome" is an appealing candidate as an initiator of genomic instability, its mutations or deletions usually occur late in the course of tumor progression. P53 may, however, be...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章,评审

    doi：10.1007/BF03343889

    authors： Farid NR

    更新日期：2001-07-01 00:00:00

  • Recombinant human thyroid-stimulating hormone (rhTSH): clinical development.

    abstract：:Recombinant human thyroid-stimulating hormone (rhTSH) was developed to safely provide exogenous TSH stimulation in patients on thyroid hormone suppression therapy (THST), which is integral to long-term management of well-differentiated thyroid cancer. Such stimulation allows detection of thyroid remnant and neoplastic...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章,评审

    doi：

    authors： Incerti C

    更新日期：1999-01-01 00:00:00

  • Somatostatin-related therapeutics in ophthalmology: a review.

    abstract：:Somatostatin and its derivatives have been predominantly studied and succesfully used in endocrinological diseases. This article reviews the rationale of the use of somatostatin and its derivatives in ophthalmology based on current understanding of its action in the eye and summarizes previously published controlled s...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章,评审

    doi：

    authors： Missotten T,Baarsma GS,Kuijpers RW,van den Born LI,van der Loos T,Croxen R,Hofland LJ,van Hagen PM

    更新日期：2005-01-01 00:00:00

  • Persistent pituitary resistance to thyroid hormone in congenital versus later-onset hypothyroidism.

    abstract：:The pituitary and peripheral responses to L-T4 and L-T3 therapy were studied in 12 patients with congenital goitrous hypothyroidism, in 10 patients with an ectopic thyroid and onset of hypothyroidism at 3-8 years of age, and in 6 patients with adult-onset hypothyroidism, after they had had their chronic thyroid hormon...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03348554

    authors： Cavaliere H,Medeiros-Neto GA,Rosner W,Kourides IA

    更新日期：1985-12-01 00:00:00

  • The inhibitory effects of PKCθ on adiponectin expression is mediated by ERK in 3T3-L1 adipocytes.

    abstract：:The research suggests that adiponectin plays an important role in sensitizing insulin action. It is interesting to find that the lower levels of adiponectin exist in the plasma of obese and Type 2 diabetes subjects and in the adipose tissue of obese, db/db mice, and insulin-resistant individuals. However, the underlyi...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03346688

    authors： Sun S,Liu Y,Lu J,Omar A,Sun S,Bi Y,Wang C

    更新日期：2011-01-01 00:00:00

  • Diet influences the content of bioactive peptides in goat milk.

    abstract：:Leptin, insulin, ghrelin, and IGF-I are circulating peptide hormones concerned with energy homeostasis and the regulation of GH axis. They are present in human milk, and are thought to promote neonatal development. The aim of the present study was to detect these substances in goat milk and determine whether their lev...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03346493

    authors： Rosi F,Aufy AA,Magistrelli D

    更新日期：2009-06-01 00:00:00

  • Calcitonin serum levels in different pituitary diseases. A preliminary report.

    abstract：:The calcitonin (CT) concentration has been determined in the serum of 17 patients with different pituitary diseases. More or less elevated levels were found in 7 of them. No definite correlation has been observed between the immunoreactive CT and the different pituitary hormones. ...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03348221

    authors： Ziliotto D,Luisetto G,Cecchini M,Gastaldo M,Francia G,Padovan L,Heynen G

    更新日期：1980-01-01 00:00:00

  • Phosphaturic mesenchymal tumors: what an endocrinologist should know.

    abstract：:Tumor-induced osteomalacia (TIO), also known as "oncogenic osteomalacia", is a rare cause of osteomalacia. TIO often has an insidious onset characterized clinically by progressive muscle weakness and bone pain with fractures. The hallmark biochemical finding is a persistent low serum phosphorus concentration due to re...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章,评审

    doi：10.1007/s40618-018-0849-5

    authors： Boland JM,Tebben PJ,Folpe AL

    更新日期：2018-10-01 00:00:00

  • Asymmetric dimethylarginine levels in thyroid diseases.

    abstract：:Thyroid diseases may lead to endothelial dysfunction; however, the mechanism underlying the endothelial dysfunction in thyroid disease is not clear yet. Asymmetric dimethylarginine (ADMA), a novel inhibitor of endothelial nitric oxide synthase (eNOS), blocks nitric oxide (NO) synthesis from L-arginine. Symmetric dimet...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03347423

    authors： Arikan E,Karadag CH,Guldiken S

    更新日期：2007-03-01 00:00:00

  • Hypercalciuria: its value as a predictive risk factor for nephrolithiasis in asymptomatic primary hyperparathyroidism?

    abstract：CONTEXT:The latest guidelines of the 4th International Workshop on Asymptomatic Primary Hyperparathyroidism (aPHPT) reintroduced hypercalciuria (i.e. urinary calcium > 400 mg/day) as criterion for surgery. However, the value of hypercalciuria as a predictor of nephrolithiasis and the correct cut-off values still need t...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/s40618-019-01162-y

    authors： Saponaro F,Cetani F,Mazoni L,Apicella M,Di Giulio M,Carlucci F,Scalese M,Pardi E,Borsari S,Bilezikian JP,Marcocci C

    更新日期：2020-05-01 00:00:00

  • Management of peritoneal hemorrhage due to follicle rupture under anticoagulation therapy.

    abstract：:Severe bleeding into the peritoneal cavity from a ruptured ovulatory follicle is a rare complication in women receiving anticoagulation therapy. A number of both surgical and non surgical methods have been proposed to solve this problem. We adopted a novel therapeutic approach in a 40-year old patient who presented wi...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03348998

    authors： Ulrich U,Rossmanith WG

    更新日期：1994-05-01 00:00:00

  • Effects of hypothyroidism and endocrine disruptor-dependent non-thyroidal illness syndrome on the GnRH-gonadotroph axis of the adult male rat.

    abstract：:Effects of primary hypothyroidism (HYPO) on the male gonadal axis are controversial, with only scanty data on the gonadotroph cell response and no information on GnRH tuberoinfundibular neurons, even in animal models. HYPO has been reported to variably induce hypogonadotropic hypogonadism, a hypergonadotropic state, o...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章,评审

    doi：

    authors： Toni R,Della Casa C,Castorina S,Cocchi D,Celotti F

    更新日期：2005-01-01 00:00:00

  • The treatment with growth hormone receptor antagonist in acromegaly: effect on vascular structure and function in patients resistant to somatostatin analogues.

    abstract：BACKGROUND:Acromegaly is known to be associated to vascular damage characterized by an increase of vascular wall thickness and an impairment of vascular function. AIM:The aim of this study was to evaluate the effect of medical treatment with the GH receptor antagonist pegvisomant on vascular structure and function in ...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03346667

    authors： De Martino MC,Auriemma RS,Brevetti G,Vitale G,Schiano V,Galdiero M,Grasso L,Lombardi G,Colao A,Pivonello R

    更新日期：2010-10-01 00:00:00

  • Correction to: Radioiodine ablation with 1,850 MBq in association with rhTSH in patients with differentiated thyroid cancer.

    abstract：:Unfortunately, the fourth author's middle name was missed out in the original publication of this article. The complete correct name should read as follows. ...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章,已发布勘误

    doi：10.1007/s40618-018-0883-3

    authors： Tresoldi AS,Sburlati LF,Rodari M,Schinkelshoek MS,Perrino M,De Leo S,Montefusco L,Colombo P,Arosio M,Lania AGA,Fugazzola L,Chiti A

    更新日期：2018-08-01 00:00:00

  • A comparison of the direct immunobead test and other tests for sperm antibodies detection.

    abstract：:The authors report the results of a correlation study between the direct Immunobead Test (d-IBT) and other techniques for antisperm antibodies detection. The Gelatin Agglutination Test (GAT) and Tray Agglutination Test (TAT) were used to detect antibodies in blood serum and seminal plasma ("indirect methods"). The Dir...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03346835

    authors： Dondero F,Lenzi A,Gandini L,Lombardo F,Culasso F

    更新日期：1991-06-01 00:00:00

  • Energy expenditure in the etiology of human obesity: spendthrift and thrifty metabolic phenotypes and energy-sensing mechanisms.

    abstract：:The pathogenesis of human obesity is the result of dysregulation of the reciprocal relationship between food intake and energy expenditure (EE), which influences daily energy balance and ultimately leads to weight gain. According to principles of energy homeostasis, a relatively lower EE in a setting of energy balance...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章,评审

    doi：10.1007/s40618-017-0732-9

    authors： Piaggi P,Vinales KL,Basolo A,Santini F,Krakoff J

    更新日期：2018-01-01 00:00:00

  • Pheochromocytoma combined with pre-clinical Cushing's syndrome in the same adrenal gland.

    abstract：:Pheochromocytoma (PHEO) occasionally associates with pathological lesions of the adrenal cortex. In most of them, ectopic adrenocorticotropic hormone (ACTH) produced by PHEO resulted in bilateral adrenocortical hyperplasia. The coexistence of PHEO and pre-clinical Cushing's syndrome (PCS) of the same adrenal gland has...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03347247

    authors： Erem C,Hacihasanoglu A,Ersöz HO,Reis AK,Calik A,Ukinç K,Koçak M

    更新日期：2005-06-01 00:00:00

  • The association between circulating irisin levels and different phenotypes of polycystic ovary syndrome.

    abstract：PURPOSE:The diagnosis of polycystic ovary syndrome (PCOS) is based on a combination of various clinical phenotypes in each patient. However, insulin resistance (IR) and dysmetabolism are not included in the diagnostic criteria of PCOS. Therefore, the definition of PCOS is controversial. The objective of this study is t...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/s40618-018-0902-4

    authors： Zhang L,Fang X,Li L,Liu R,Zhang C,Liu H,Tan M,Yang G

    更新日期：2018-12-01 00:00:00

  • Intranasal and intramuscular human calcitonin in female osteoporosis and in Paget's disease of bones: a pilot study.

    abstract：:It has been shown that human calcitonin (hCT) is absorbed through the nasal mucosa when administered together with promoters like sodium glycocholate (SGC) or dihydrofusinate. The aim of this study was to compare the clinical and metabolic effect of intranasal (in) and intramuscular (im) hCT in patients with osteoporo...

    journal_title：Journal of endocrinological investigation

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1007/BF03350260

    authors： Pontiroli AE,Pajetta E,Calderara A,Alberetto M,Pozza G,Manganelli V,Resmini G,Tessari L,Maresca V

    更新日期：1991-01-01 00:00:00

  • Reference ranges for an automated chemiluminescent assay for serum insulin-like growth factor I (IGF-I) in a large population of healthy adults from Buenos Aires.

    abstract：PURPOSE:Insulin-like growth factor I (IGF-I) represents an essential tool in the diagnostic work-up and in the monitoring of treatment efficacy for somatotrophic axis disorders both in children and adults. A large number of factors including, but not limited to, age, sex and weight as well as analytical variables influ...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/s40618-015-0265-z

    authors： Guitelman M,Smithuis F,Garcia Basavilbaso N,Aranda C,Fabre B,Oneto A

    更新日期：2015-09-01 00:00:00

  • Alterations in circulating thyroid hormones and thyroxine-binding globulin levels during diabetic ketoacidosis.

    abstract：:During diabetic ketoacidosis, in 17 adult patients, significant decreases in serum TBG and total T4 levels were observed, without significant alteration of the T4 to TBG binding property. In addition, serum free T4 (FT4) was moderately elevated and the TSH response to TRH was markedly blunted. No correlation, however,...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03348220

    authors： Glinoer D,Naeije R,Golstein J,Fernandez-Deville M,Vanhaelst L

    更新日期：1980-01-01 00:00:00

  • Human resistin gene polymorphism is associated with visceral obesity and fasting and oral glucose stimulated C-peptide in the Québec Family Study.

    abstract：:Obesity and insulin resistance are common features of Type 2 Diabetes. A new protein called resistin has been shown to be secreted by adipocytes in mice and to influence insulin sensitivity. The goal of the present study was to investigate the associations between one polymorphism (g-420C>G) of the human resistin gene...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03345301

    authors： Bouchard L,Weisnagel SJ,Engert JC,Hudson TJ,Bouchard C,Vohl MC,Pérusse L

    更新日期：2004-12-01 00:00:00

  • Cabergoline therapy and the risk of cardiac valve regurgitation in patients with hyperprolactinemia: a meta-analysis from clinical studies.

    abstract：:Dopamine agonists have been associated with increased risk of cardiac valve regurgitation in patients with Parkinson's disease. Whether these drugs might be harmful for patients with hyperprolactinemia is still unsettled. Occasional case reports and 7 studies on the relationship between cabergoline and cardiac valve r...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章,meta分析,评审

    doi：10.1007/BF03345662

    authors： Bogazzi F,Manetti L,Raffaelli V,Lombardi M,Rossi G,Martino E

    更新日期：2008-12-01 00:00:00

  • Effects of metabolic abnormalities, hyperandrogenemia and clomiphene on liver function parameters among Chinese women with polycystic ovary syndrome: results from a randomized controlled trial.

    abstract：PURPOSE:To investigate the effects of metabolic abnormalities, hyperandrogenemia and ovulation induction by clomiphene/acupuncture on liver function parameters among women with polycystic ovary syndrome (PCOS). METHODS:This is a secondary analysis of a randomized controlled trial. All 1000 subjects were diagnosed as P...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/s40618-018-0953-6

    authors： Cai WY,Gao JS,Luo X,Ma HL,Ge H,Liu N,Xia Q,Wang Y,Han BW,Wu XK

    更新日期：2019-05-01 00:00:00

  • Hypophysitis superimposed on a non-functioning pituitary adenoma: diagnostic clinical, endocrine, and radiologic features.

    abstract：:Pituitary adenomas are common neoplasms requiring medical and/or surgical treatment when associated with hormonal hypersecretion. Treatment of non-functioning pituitary adenomas is necessary when symptoms of mass effect or hormonal deficits occur. However, therapeutic options, including surgical resection and/or radio...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03347449

    authors： Ballian N,Chrisoulidou A,Nomikos P,Samara C,Kontogeorgos G,Kaltsas GA

    更新日期：2007-09-01 00:00:00

  • Seasonal plasma profiles of testosterone and androstenedione in the Gentile di Puglia ram in southern Italy.

    abstract：:Seasonal plasma testosterone and androstenedione levels were determined by radioimmunoassay in the Gentile di Puglia ram. Androstenedione remains steady throughout the years, while testosterone shows seasonal changes. Testosterone is at high levels in winter, spring and summer and drops to a low concentration in autum...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03348490

    authors： Dell'Aquila S,Crasto A,Alberico G,Varriale B,Pelosi A,Pierantoni R

    更新日期：1985-06-01 00:00:00

  • Puerperal breast feeding does not stimulate circulating opioids in humans.

    abstract：:ACTH, beta lipotropin (beta LPH), beta endorphin (beta EP), Prolactin (PRL) and Cortisol were measured in the first five days of puerperium at 9:00, before and 30 minutes after suckling, in 7 healthy lactating women. With the exception of PRL plasma levels which decline, although remaining at high concentrations durin...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03350534

    authors： Genazzani AR,Facchinetti F,Parrini D,Petraglia F,La Rosa R,Puggioni R,D'Antona N

    更新日期：1982-11-01 00:00:00

  • Estriol affects prolactin and LH secretion in rats.

    abstract：:The effects of estriol on serum prolactin (PRL) and LH levels, on the pituitary response to TRH and LHRH and on the synthesis and release of PRL from the anterior pituitary gland were investigated in female rats. The increase of serum PRL levels after estradiol administration was found to be associated with an increas...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03349902

    authors： Diaz MC,Seilicovich A,Duvilanski BH,Siseles N,Lasaga M,Debeljuk L

    更新日期：1989-01-01 00:00:00

  • Effects of starvation on pituitary and plasma growth hormone in rats.

    abstract：:The present experiments were undertaken to study the effect of complete food removal on body weight, pituitary growth hormone (GH), plasma GH and glucose concentration in male and female Wistar rats. Plasma and pituitary GH levels were measured by means of a specific radioimmunoassay. Plasma glucose concentration decr...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03349417

    authors： Gonzalez C,Jolin T

    更新日期：1981-01-01 00:00:00