Abstract:
:Recently, highly purified bovine thyrotropin (bTSH) of pituitary origin, as well as recombinant human (h) TSH free of lutropin (LH) contamination, has been reported to activate the LH/choriogonadotropin receptor (LH/CGR). These data challenge the concept of TSH specificity for its own receptor. We, therefore, re-evaluated these data using, as targets, the recombinant hTSH and rat LH/CGRs stably expressed in Chinese hamster ovary (CHO) cells. Partially purified bTSH (2 IU/mg protein) and, to a lesser degree, highly purified bTSH (30 IU/mg protein) increased intracellular cAMP levels in CHO-LH/CGR cells (an EC50 of 0.2 and > 20 mIU/ml, respectively). In contrast, recombinant hTSH (up to 1 IU/ml) did not. All three TSH preparations increased cAMP levels to the same extent in CHO-TSHR cells (an EC50 of 0.3 mIU/ml). Furthermore, we observed only nonspecific, low affinity TSH binding for CHO-LH/CGR cells and also for CHO cells transfected with the expression vector alone (a Kd of 100 nM), although both high and low affinity TSH binding was demonstrated in CHOT-SHR cells (a Kd of 0.3 and 100 nM, respectively). These data indicate that even highly purified bTSH of pituitary origin contains significant amounts of LH, and that TSH itself does not appear to activate the LH/CGR.
journal_name
J Endocrinol Investjournal_title
Journal of endocrinological investigationauthors
Nagayama Y,Yamasaki H,Takeshita A,Kimura H,Ashizawa K,Yokoyama N,Yamashita S,Rapoport B,Nagataki Sdoi
10.1007/BF03347814subject
Has Abstractpub_date
1995-04-01 00:00:00pages
283-7issue
4eissn
0391-4097issn
1720-8386journal_volume
18pub_type
杂志文章abstract::Aging is related to critical changes of the hypothalamo-pituitary-adrenal function. A decline in serum DHEA levels has been demonstrated in healthy elderly subjects, while ACTH and cortisol concentrations remain at normal values. The purpose of the present study was to investigate the effect of aging on pituitary-adre...
journal_title:Journal of endocrinological investigation
pub_type: 临床试验,杂志文章
doi:10.1007/BF03347325
更新日期:1998-07-01 00:00:00
abstract::This report describes the results of the second part of the collaborative study organized by a working group sponsored by the Community Bureau of Reference of the European Community Commission. The whole study was designed to understand the causes of discrepancy among LH immunoassay methods. In the parent report we de...
journal_title:Journal of endocrinological investigation
pub_type: 临床试验,杂志文章
doi:10.1007/BF03347727
更新日期:1994-06-01 00:00:00
abstract:BACKGROUND:Adiponectin, a fat derived cytokine, is a potential independent contributor to bone mineral density (BMD); however, its action on bone metabolism in humans is still unclear. AIM:The aim of this study was to investigate the relationship of adiponectin with bone mass indices and bone metabolic markers in midd...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.3275/7744
更新日期:2012-03-01 00:00:00
abstract::The syndrome of apparent mineralocorticoid syndrome (AME) results from defective 11beta-hydroxysteroid dehydrogenase 2 (11beta-HSD2). This enzyme is co-expressed with the mineralocorticoid receptor (MR) in the kidney and converts cortisol to its inactive metabolite cortisone. Its deficiency allows the unmetabolized co...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03343755
更新日期:2000-07-01 00:00:00
abstract::A 33-year-old female presented in 1966 with striking pigmentation, typical of Addison's disease, and amenorrhea. Endocrine assessment then showed normal basal serum cortisol and urinary hydroxysteroid levels, but serum cortisol did not respond to stimulation with either exogenous ACTH or lysine vasopressin. Steroid re...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03349957
更新日期:1989-03-01 00:00:00
abstract::Over the last decade, there has been an alarming increase of Type 2 diabetes mellitus (T2DM) in youths, concomitant with the rise of obesity in this age group. T2DM is a progressive disease with a gradual increase in insulin resistance associated later with a decline in insulin secretion with fasting hyperglycemia. Pr...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:10.1007/BF03347581
更新日期:2005-10-01 00:00:00
abstract::Orbital fibroblasts exhibit a unique phenotype including exaggerated responses to proinflammatory cytokines. We hypothesize that the unusual susceptability of these fibroblasts to molecular cues underlies the involvement of the orbit in Graves' ophthalmopathy. A number of attributes of orbital fibroblasts are reviewed...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:10.1007/BF03345273
更新日期:2004-03-01 00:00:00
abstract::The IGFs are ubiquitous and have pleoitropic effects. They are critical for normal growth and development, and for normal functioning of adult tissues. A liver-specific gene-deletion knockout of the IGF-I gene resulted in a mouse model with reduced circulating IGF-I levels, that led to insulin resistance due to the se...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:
更新日期:2005-01-01 00:00:00
abstract::To analyze the inhibitory effect of hormone replacement therapy (HRT) on bone resorption, we measured urinary excretion of C-telopeptide (CTX) and N-telopeptide (NTX) of type I collagen as new markers of bone resorption and assessed their correlation with bone mineral density, in comparison with urinary pyridinoline (...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03347294
更新日期:1998-03-01 00:00:00
abstract:BACKGROUND:Results of circulating Ghrelin levels in hyper- or hypothyroidism are conflicting and only overt thyroid dysfunction has been evaluated. AIM:To evaluate in a large number of patients with thyroid disfunction whether: a) hyper- and hypothyroidism (clinical or subclinical) are associated with variations in bo...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03345683
更新日期:2009-01-01 00:00:00
abstract::Testosterone therapy has been reported to be useful in the treatment of hypogonadism and partial androgen deficiency of the aging male (PADAM) syndrome. Testosterone administration is needed in order to maintain secondary sexual characteristics, muscle mass, bone mineral density, cognitive function and sexual drive. N...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:
更新日期:2005-01-01 00:00:00
abstract::Adolescent girls with a history of premature pubarche have an increased incidence of functional ovarian hyperandrogenism [a form of polycystic ovary syndrome (PCOS)] at adolescence, which is usually associated with hyperinsulinemia and dyslipemia. The hyperinsulinemia and lipid disturbances can often be detected in th...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:10.1007/BF03350781
更新日期:1998-10-01 00:00:00
abstract::Insulin degradation in IM-9 cultured human lymphocytes occurs extracellularly. In the presence of 0.1% bovine serum albumin, insulin degradation products are formed that are soluble in 5% trichloroacetic acid. In the presence of 5% bovine serum albumin, the major products of insulin degradation are insoluble in 5% tri...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03348223
更新日期:1980-01-01 00:00:00
abstract::Evaluation of thyroid status by measurement of free thyroid hormone concentrations seems particularly helpful in conditions with altered serum binding proteins. In pregnancy, a condition of increased thyroxine binding globulin, serum free thyroxine (FT4) and free triiodothyronine (FT3) concentrations have been reporte...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03350244
更新日期:1991-01-01 00:00:00
abstract::The occurrence of a genetic background in the etiology of polycystic ovarian syndrome (PCOS) represents the rational basis to postulate the existence of a male PCOS equivalent. Hormonal and metabolic abnormalities have been described in male relatives of women with PCOS. These males also have a higher prevalence of ea...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:10.1007/s40618-017-0728-5
更新日期:2018-01-01 00:00:00
abstract::E-cadherin and catenins play a major role in neoplastic cell behavior as a suppressor of invasion and/or metastasis. The aim of this study was to determine E-cadherin, alpha-catenin and beta-catenin expressions in papillary thyroid carcinoma (PTC) and to correlate the results of expression to initial clinicopathologic...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03345079
更新日期:2002-07-01 00:00:00
abstract::Not only thyroid adenomas and carcinomas, but also the majority of single and well delimited goiter nodules, even if morphologically heterogeneous, are of clonal origin. However, it is still unknown whether the nodules of rapidly growing, recurrent goiters are clonal or polyclonal. We investigated by PCR-based analysi...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03350774
更新日期:1998-06-01 00:00:00
abstract:CONTEXT:The latest guidelines of the 4th International Workshop on Asymptomatic Primary Hyperparathyroidism (aPHPT) reintroduced hypercalciuria (i.e. urinary calcium > 400 mg/day) as criterion for surgery. However, the value of hypercalciuria as a predictor of nephrolithiasis and the correct cut-off values still need t...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/s40618-019-01162-y
更新日期:2020-05-01 00:00:00
abstract::There has been a substantial increase in women practicing sports over the past 30 yr. While exercise provides many health benefits, there appears to be a unique set of risks associated with intense exercise for the female athlete. The female athlete triad encompasses these risks, including amenorrhea, osteoporosis and...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:10.1007/BF03345238
更新日期:2003-09-01 00:00:00
abstract::We report an original application of quantitative reverse transcription-polymerase chain reaction (Q.RT-PCR) for the evaluation of atrial natriuretic factor (ANF), brain natriuretic peptide (BNP) and beta-actin mRNA in small atrial samples (10-15 mg). A fixed amount of total RNA was simultaneously reverse transcribed ...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03343655
更新日期:1999-12-01 00:00:00
abstract:PURPOSE:Intravenous glucocorticoids (ivGCs) given as 12-weekly infusions are the first-line treatment for moderate-to-severe and active Graves' orbitopathy (GO), but they are not always effective. In this study, we evaluated whether response at 6 weeks correlated with outcomes at 12 (end of intervention) and 24 (follow...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/s40618-017-0608-z
更新日期:2017-05-01 00:00:00
abstract::Thyroid diseases may lead to endothelial dysfunction; however, the mechanism underlying the endothelial dysfunction in thyroid disease is not clear yet. Asymmetric dimethylarginine (ADMA), a novel inhibitor of endothelial nitric oxide synthase (eNOS), blocks nitric oxide (NO) synthesis from L-arginine. Symmetric dimet...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03347423
更新日期:2007-03-01 00:00:00
abstract::We studied the possibility of an association between serum somatomedin-C (Sm-C) and thyroid hormone concentrations. For this purpose 34 hyperthyroid patients, 39 patients with primary hypothyroidism, 36 patients with severe nonthyroidal illnesses (NTI), and 63 euthyroid healthy control subjects were examined. The mean...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03349528
更新日期:1990-02-01 00:00:00
abstract::Vitamin D via its receptor has essential actions on parathyroid cells, inhibiting PTH secretion, and parathyroid cell proliferation. While the effects of vitamin D depletion in the pathogenesis of secondary hyperparathyroidism in elderly individuals or in the occurrence of parathyroid hyperplasia in patients with rena...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:
更新日期:2011-07-01 00:00:00
abstract::We studied 8 insulin dependent stable young diabetics with a mean age of 20 yr. Each patient received 10 mg of diazepam orally after with drawing the baseline blood sample. Subsequent samples were obtained at 30 min intervals for 150 min. The oral administration of diazepam resulted in a significant increase in serum ...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03349471
更新日期:1982-05-01 00:00:00
abstract::Seventy years after the first successful radioiodine treatment of metastatic differentiated thyroid cancer (DTC), radioiodine (131I) therapy for this type of tumor is still without alternative. During the last decade, some key issues such as individual dosimetry, and preparation of 131I therapy by recombinant human TS...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:
更新日期:2012-01-01 00:00:00
abstract::Aim of the study was to compare urinary galactosyl-hydroxylysine (GHyl), deoxypyridinoline (D-Pyr) and pyridinoline (Pyr) before and after 5 to 9 months of hormone replacement therapy (HRT) in postmenopausal women. The urinary markers were measured by HPLC in the second void of fasting samples and were expressed as ra...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03349858
更新日期:1996-03-01 00:00:00
abstract::A longitudinal study was carried out in an area of endemic goiter of north-eastern Sicily. Three different surveys (in 1977, 1983 and 1994) evaluated the epidemiological prevalence of goiter in schoolchildren of the endemic area (towns of Bronte, Troina and Maniaci) in comparison to an iodine sufficient control area (...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03349031
更新日期:1996-10-01 00:00:00
abstract:OBJECTIVE:To our knowledge, no case of remission in autoimmune Addison's disease has previously been reported. We describe a patient with primary adrenal insufficiency caused by autoimmune adrenalitis in whom partial remission was observed after 7 yr. CASE:A 39-yr-old male was referred because of extreme fatigue, weig...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03345623
更新日期:2008-07-01 00:00:00
abstract::Anorexia nervosa (AN) is an eating disorder characterized by self-induced starvation due to fear of adiposity. Ghrelin, gastric peptide with potent orexigenic, adipogenic, GH-releasing and metabolic properties, is elevated in AN. We have previously shown that intervention with exogenous ghrelin is not effective in ter...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03350816
更新日期:2007-10-01 00:00:00