A rapid semi-automated method of determining total estrogens in non-pregnancy urine.

Abstract:

:A rapid routine method of determining estrogens in non-pregnancy urine is described. It involves the following steps: 1) Dilution of each urine sample with an equal volume of saturated ammonium sulfate solution; 2) Addition of Sephadex G-15 beads; 3) Filtration of the mixture; 4) Liberation of the estrogens from the Sephadex by washing the beads on the filter with 0.1N NaOH; 5) Automated fluorimetric analysis of the alkaline filtrate according to the procedure used for the determination of estrogen in pregnancy urine. A correction for non-specific fluorescence remains necessary and is achieved by measuring background fluorescence with a second fluorimeter.

journal_name

J Endocrinol Invest

authors

Gebhardt DO,Soederhuizen W,Moolenaar AJ

doi

10.1007/BF03350365

subject

Has Abstract

pub_date

1978-04-01 00:00:00

pages

163-6

issue

2

eissn

0391-4097

issn

1720-8386

journal_volume

1

pub_type

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