Abstract:
BACKGROUND:The differential expression of virulence genes is often used by microbial pathogens in adapting to the environment of their host. The differential expression of such sets of genes can be regulated by RNA polymerase sigma factors. Some sigma factors are differentially expressed, which can provide a means to identifying other differentially expressed genes such as those whose expression are controlled by the sigma factor. METHODS:To identify sigma factor-regulated genes, we developed a method, termed I-TRAP, for the identification of transcriptional regulator activated promoters. The I-TRAP method is based on the fact that some genes will be differentially expressed in the presence and absence of a transcriptional regulator. I-TRAP uses a DNA library in a promoter-trap vector that contains two reporter genes, one to allow the selection of active promoters in the presence of the transcriptional regulator and a second to allow screening for promoter activity in the absence of the transcriptional regulator. RESULTS:To illustrate the development and use of the I-TRAP approach, the construction of the vectors, host strains, and library necessary to identify SigmaE-regulated genes of Mycobacterium tuberculosis is described. CONCLUSION:The I-TRAP method should be a versatile and useful method for identifying and characterizing promoter activity under a variety of conditions and in response to various regulatory proteins. In our study, we isolated 360 clones that may contain plasmids carrying SigmaE-regulated promoters genes of M. tuberculosis.
journal_name
BMC Infect Disjournal_title
BMC infectious diseasesauthors
McLendon MM,Shinnick TMdoi
10.1186/1471-2334-3-15keywords:
subject
Has Abstractpub_date
2003-07-11 00:00:00pages
15issn
1471-2334journal_volume
3pub_type
杂志文章abstract:BACKGROUND:The role of the surface capsular polysaccharides (CPs) of Mycobacterium tuberculosis (Mtb) in the pathogenesis of infection and disease, as well their potential for use as diagnostic reagents and vaccine antigens, are unknown. METHODS:Serum antibody to two CPs of Mtb, arabinomannan (AM) and glucan (Glu), we...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-13-276
更新日期:2013-06-19 00:00:00
abstract:BACKGROUND:Egypt ranks fifth for the burden of viral hepatitis worldwide. As part of Egypt's renewed national strategy for the elimination of viral hepatitis, surveillance for acute viral hepatitis (AVH) was re-established during 2014-2017 to describe the current epidemiology and associated risk factors, and changes fr...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-3806-9
更新日期:2019-02-14 00:00:00
abstract:BACKGROUND:In West and Central Africa areas of endemic Loa loa infections overlap with regions of high prevalence of human immunodeficiency virus type 1 (HIV-1) infections. Because individuals in this region are exposed to filarial parasites from birth, most HIV-1 infected individuals invariably also have a history of ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3072-2
更新日期:2018-04-04 00:00:00
abstract:BACKGROUND:The potential impact of an influenza pandemic can be assessed by calculating a set of transmissibility parameters, the most important being the reproduction number (R), which is defined as the average number of secondary cases generated per typical infectious case. METHODS:We conducted a systematic review t...
journal_title:BMC infectious diseases
pub_type: 杂志文章,评审
doi:10.1186/1471-2334-14-480
更新日期:2014-09-04 00:00:00
abstract:BACKGROUND:In order to compare the transmissibility of the 2009 pH1N1 pandemic during successive waves of infections in summer and fall/winter in the Northern Hemisphere, and to assess the temporal changes during the course of the outbreak in relation to the intervention measures implemented, we analyze the epidemiolog...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-11-332
更新日期:2011-12-02 00:00:00
abstract:BACKGROUND:Information about improvements in the health status of population at-risk of helminth infection after anthelminthic treatment helps to evaluate the effectiveness of the large scale deworming program. The objectives of this study were to assess the impact of anthelminthic treatment on the prevalence and inten...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1956-6
更新日期:2016-10-28 00:00:00
abstract:BACKGROUND:Giardia duodenalis is a flagellate parasite which has been considered the most common protozoa infecting human worldwide. Molecular characterization of G. duodenalis isolates have revealed the existence of eight groups (Assemblage A to H) which differ in their host distribution. Assemblages A and B are found...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-14-78
更新日期:2014-02-12 00:00:00
abstract:BACKGROUND:Diagnosis of bloodstream infections (BSI) in neonates is usually difficult due to minimal symptoms at presentation; thus early empirical therapy guided by local antibiotic susceptibility profile is necessary to improve therapeutic outcomes. METHODS:A review of neonatal blood cultures submitted to the microb...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1913-4
更新日期:2016-10-24 00:00:00
abstract:BACKGROUND:Neonatal bloodstream infection (BSI) is the most important cause of morbidity and mortality in the neonatal intensive care unit (NICU). Although most neonatal BSIs are primary bacteremia, some are associated with a focus of infection. This distinction is not well characterized. METHODS:All patients with neo...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-017-2574-7
更新日期:2017-07-03 00:00:00
abstract:BACKGROUND:It is believed that the current prevalence of malaria in endemic areas reflects selection for the carrier form of sickle cell trait through a survival advantage. Malaria has been incriminated as a great cause of mortality in people with sickle cell disease (SCD). However, people with SCD, a high-risk group, ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4757-x
更新日期:2020-01-14 00:00:00
abstract:BACKGROUND:Although live-attenuated varicella-zoster virus (VZV) vaccines have been proven to be safe and effective in preventing varicella and real-word evidence shows routine childhood immunization programs are effective in dramatically reducing varicella associated morbidity and mortality, varicella vaccine is not i...
journal_title:BMC infectious diseases
pub_type: 杂志文章,多中心研究
doi:10.1186/s12879-017-2575-6
更新日期:2017-07-14 00:00:00
abstract:BACKGROUND:Tuberculosis (TB) continues to be a public health challenge in Saudi Arabia, particularly for the elderly. This study was conducted to estimate mortality per 1000 person-year among TB and resistant TB cases and to identifying factors associated with mortality. METHODS:This is a retrospective cohort study of...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4063-7
更新日期:2019-05-16 00:00:00
abstract:BACKGROUND:Fear of TB infection is rooted in historical and social memories of the disease, marked by stigma, segregation and exclusion. Healthcare workers (HCWs) face these same fears today, and even seek to hide their TB status when infected. This study sought to investigate factors associated with HCWs fears of acqu...
journal_title:BMC infectious diseases
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12879-019-4115-z
更新日期:2019-05-28 00:00:00
abstract:BACKGROUND:Hand, foot and mouth disease (HFMD) is a major communicable disease in children ≤6 years old, particularly in several countries in the Asia-Pacific Region, including Thailand. HFMD impacts public health and the economy, especially in northern Thailand. METHODS:A prospective cohort study was conducted to est...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3560-4
更新日期:2018-12-06 00:00:00
abstract:BACKGROUND:Chronic hepatitis C virus (HCV) infection can progress to cirrhosis and end-stage liver disease in a substantial proportion of patients. The infection is frequently asymptomatic, leaving many infected individuals unaware of the diagnosis until complications occur. This advocates the screening of healthy indi...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-3832-7
更新日期:2019-02-26 00:00:00
abstract:BACKGROUND:The State of Ceará, in Northeastern Brazil, suffers from a triple burden of arboviruses (dengue, Zika and chikungunya). We measured the seroprevalence of chikungunya, dengue and Zika and its associated factors in the population of Juazeiro do Norte, Southern Ceará State, Brazil. METHODS:A cross-sectional st...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05611-5
更新日期:2020-11-24 00:00:00
abstract:BACKGROUND:Chikungunya fever is an emerging arboviral disease characterized by an algo-eruptive syndrome, inflammatory polyarthralgias, or tenosynovitis that can last for months to years. Up to now, the pathophysiology of the chronic stage is poorly understood. CASE PRESENTATION:We report the first case of CHIKV infec...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-9-200
更新日期:2009-12-10 00:00:00
abstract:BACKGROUND:The Belgian HIV epidemic is largely concentrated among men who have sex with men and Sub-Saharan Africans. We studied the continuum of HIV care of those diagnosed with HIV living in Belgium and its associated factors. METHODS:Data on new HIV diagnoses 2007-2010 and HIV-infected patients in care in 2010-2011...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-1230-3
更新日期:2015-11-03 00:00:00
abstract:BACKGROUND:Saksenaea species (spp.) are uncommon causes of mucormycosis but are emerging pathogens mostly associated with trauma and soil contamination often in immunocompetent hosts. Due to lack of sporulation in the laboratory, diagnosis and susceptibility testing is difficult so optimal treatment regimens are unknow...
journal_title:BMC infectious diseases
pub_type: 杂志文章,评审
doi:10.1186/s12879-020-05459-9
更新日期:2020-10-14 00:00:00
abstract:BACKGROUND:In the spring of 1918, the "War to End All Wars", which would ultimately claim more than 37 million lives, had entered into its final year and would change the global political and economic landscape forever. At the same time, a new global threat was emerging and would become one of the most devastating glob...
journal_title:BMC infectious diseases
pub_type: 历史文章,杂志文章,评审
doi:10.1186/s12879-019-3750-8
更新日期:2019-02-06 00:00:00
abstract:BACKGROUND:The ocular manifestations of HIV may lead to visual impairment or blindness. In India, patients typically initiate antiretroviral treatment (ART) with low CD4 cell counts when the risk of ocular complications may be high. The objective of this study was to determine the prevalence and types of HIV-associated...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-9-158
更新日期:2009-09-23 00:00:00
abstract:BACKGROUND:We analyzed the results of a 3-year surveillance study on the epidemiological and clinical characteristics of healthcare associated-infections (HAIs) in elderly inpatients in a large tertiary hospital in China. METHODS:Real-time surveillance was performed from January 1, 2015 to December 31, 2017. All HAIs ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-4840-3
更新日期:2020-02-10 00:00:00
abstract:BACKGROUND:Although Indonesia has high fatality rate of human A/H5N1 cases, epidemiological and clinical data on influenza virus circulation among humans has been limited. Within Indonesia, Bali province is of interest due to high population densities of humans, pigs and poultry. This study aims to characterize and com...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-3842-5
更新日期:2019-03-07 00:00:00
abstract:BACKGROUND:Young people are disproportionately affected by sexually transmissible infections in Australia but face barriers to accessing sexual health services, including concerns over confidentiality and, for some, geographic remoteness. A possible innovation to increase access to services is the use of telemedicine. ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-11-285
更新日期:2011-10-25 00:00:00
abstract:BACKGROUND:Patients with HIV/AIDS on Antiretroviral Therapy (ART) suffer from physical, psychological and spiritual problems. Despite international policy explicitly stating that a multidimensional approach such as palliative care should be delivered throughout the disease trajectory and alongside treatment, the effect...
journal_title:BMC infectious diseases
pub_type: 杂志文章,随机对照试验
doi:10.1186/1471-2334-12-288
更新日期:2012-11-06 00:00:00
abstract:BACKGROUND:Antimicrobial resistance (AMR) in Neisseria gonorrhoeae is an emerging global health threat. Surveillance of AMR in N. gonorrhoeae in the Western Pacific Region is important, as resistant strains have typically emerged from this region. There are sparse data regarding antibiotic susceptibility of N. gonorrho...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05532-3
更新日期:2020-11-05 00:00:00
abstract:BACKGROUND:In Tanzania, routine individual-level testing for HIV drug resistance (HIVDR) using laboratory genotyping and phenotyping is not feasible due to resource constraints. To monitor the prevention or emergence of HIVDR at a population level, WHO developed generic strategies to be adapted by countries, which incl...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-14-196
更新日期:2014-04-11 00:00:00
abstract:BACKGROUND:Seroreactivity, processes of seroconversion and seroreversion, in the context of HPV infection has been investigated in numerous studies. However, the data resulting from these studies are usually not accounted for in mathematical transmission models of various HPV types due to gaps in our understanding of t...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-13-83
更新日期:2013-02-13 00:00:00
abstract:BACKGROUND:Several in vitro studies suggested the microRNA-29 (miRNA-29) family is involved in regulating HIV-1 and modulating the expression of interleukin (IL)-32, an anti-HIV-1 cytokine. METHODS:To investigate the contribution of the miRNA-29 family to HIV-1 infection in vivo, we compared miRNA-29 expression in PBM...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-0768-4
更新日期:2015-02-12 00:00:00
abstract:BACKGROUND:The first step of designing any genome-based molecular diagnostic test is to find a specific target sequence. The modified genome comparison method is one of the easiest and most comprehensive ways to achieve this goal. In this study, we aimed to explain this method with the example of Mycobacterium tubercul...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3417-x
更新日期:2018-10-12 00:00:00