Population-Specific ACE2 Single-Nucleotide Polymorphisms Have Limited Impact on SARS-CoV-2 Infectivity In Vitro.

Abstract:

:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), employs host-cell angiotensin-converting enzyme 2 (ACE2) for cell entry. Genetic analyses of ACE2 have identified several single-nucleotide polymorphisms (SNPs) specific to different human populations. Molecular dynamics simulations have indicated that several of these SNPs could affect interactions between SARS-CoV-2 and ACE2, thereby providing a partial explanation for the regional differences observed in SARS-CoV-2 infectivity and severity. However, the significance of population-specific ACE2 SNPs in SARS-CoV-2 infectivity is unknown, as no in vitro validation studies have been performed. Here, we analyzed the impact of eight SNPs found in specific populations on receptor binding and cell entry in vitro. Except for a SNP causing a nonsense mutation that reduced ACE2 expression, none of the selected SNPs markedly altered the interaction between ACE2 and the SARS-CoV-2 spike protein (SARS-2-S), which is responsible for receptor recognition and cell entry, or the efficiency of viral cell entry mediated by SARS-2-S. Our findings indicate that ACE2 polymorphisms have limited impact on the ACE2-dependent cell entry of SARS-CoV-2 and underscore the importance of future studies on the involvement of population-specific SNPs of other host genes in susceptibility toward SARS-CoV-2 infection.

journal_name

Viruses

journal_title

Viruses

authors

Hashizume M,Gonzalez G,Ono C,Takashima A,Iwasaki M

doi

10.3390/v13010067

subject

Has Abstract

pub_date

2021-01-06 00:00:00

issue

1

issn

1999-4915

pii

v13010067

journal_volume

13

pub_type

杂志文章
  • Vampire bat rabies: ecology, epidemiology and control.

    abstract::Extensive surveillance in bat populations in response to recent emerging diseases has revealed that this group of mammals acts as a reservoir for a large range of viruses. However, the oldest known association between a zoonotic virus and a bat is that between rabies virus and the vampire bat. Vampire bats are only fo...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v6051911

    authors: Johnson N,Aréchiga-Ceballos N,Aguilar-Setien A

    更新日期:2014-04-29 00:00:00

  • Evaluating the Within-Host Dynamics of Ranavirus Infection with Mechanistic Disease Models and Experimental Data.

    abstract::Mechanistic models are critical for our understanding of both within-host dynamics (i.e., pathogen replication and immune system processes) and among-host dynamics (i.e., transmission). Within-host models, however, are not often fit to experimental data, which can serve as a robust method of hypothesis testing and hyp...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v11050396

    authors: Mihaljevic JR,Greer AL,Brunner JL

    更新日期:2019-04-27 00:00:00

  • Protoparvovirus Interactions with the Cellular DNA Damage Response.

    abstract::Protoparvoviruses are simple single-stranded DNA viruses that infect many animal species. The protoparvovirus minute virus of mice (MVM) infects murine and transformed human cells provoking a sustained DNA damage response (DDR). This DDR is dependent on signaling by the ATM kinase and leads to a prolonged pre-mitotic ...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v9110323

    authors: Majumder K,Etingov I,Pintel DJ

    更新日期:2017-10-31 00:00:00

  • Molecular characterization and phylogenetic analysis of new variants of the porcine epidemic diarrhea virus in Gansu, China in 2012.

    abstract::Between January 2012 and March 2012, the infection rates of porcine epidemic diarrhea virus (PEDV) increased substantially in vaccinated swine herds in many porcine farms in Gansu Province, China. The spike (S) glycoprotein is an important determinant for PEDV biological properties. To determine the distribution profi...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v5081991

    authors: Tian Y,Yu Z,Cheng K,Liu Y,Huang J,Xin Y,Li Y,Fan S,Wang T,Huang G,Feng N,Yang Z,Yang S,Gao Y,Xia X

    更新日期:2013-08-15 00:00:00

  • Gammaherpesviral Tegument Proteins, PML-Nuclear Bodies and the Ubiquitin-Proteasome System.

    abstract::Gammaherpesviruses like Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) subvert the ubiquitin proteasome system for their own benefit in order to facilitate viral gene expression and replication. In particular, viral tegument proteins that share sequence homology to the formylglycineamide r...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v9100308

    authors: Full F,Hahn AS,Großkopf AK,Ensser A

    更新日期:2017-10-21 00:00:00

  • Feline immunodeficiency virus (FIV) neutralization: a review.

    abstract::One of the major obstacles that must be overcome in the design of effective lentiviral vaccines is the ability of lentiviruses to evolve in order to escape from neutralizing antibodies. The primary target for neutralizing antibodies is the highly variable viral envelope glycoprotein (Env), a glycoprotein that is essen...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v3101870

    authors: Hosie MJ,Pajek D,Samman A,Willett BJ

    更新日期:2011-10-01 00:00:00

  • Characterization of Monoclonal Antibodies against HA Protein of H1N1 Swine Influenza Virus and Protective Efficacy against H1 Viruses in Mice.

    abstract::H1N1 swine influenza viruses (SIV) are prevalent in pigs globally, and occasionally emerge in humans, which raises concern about their pandemic threats. To stimulate hemagglutination (HA) of A/Swine/Guangdong/LM/2004 (H1N1) (SW/GD/04) antibody response, eukaryotic expression plasmid pCI-neo-HA was constructed and used...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v9080209

    authors: Liu Y,Li H,Xue Y,Zhao S,Li C,Qu L,Zhang Y,Liu M

    更新日期:2017-08-08 00:00:00

  • Genotypic Characterization of Epstein Barr Virus in Blood of Patients with Suspected Nasopharyngeal Carcinoma in Ghana.

    abstract::Nasopharyngeal cancer (NPC) is associated with Epstein Barr virus (EBV) infection. However different viral strains have been implicated in NPC worldwide. This study aimed to detect and characterize EBV in patients diagnosed with NPC in Ghana. A total of 55 patients diagnosed with NPC by CT scan and endoscopy were age-...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v12070766

    authors: Ayee R,Ofori MEO,Tagoe EA,Languon S,Searyoh K,Armooh L,Bilson-Amoah E,Baidoo K,Kitcher E,Wright E,Quaye O

    更新日期:2020-07-16 00:00:00

  • Identification of A Novel Picorna-Like Virus, Burpengary Virus, that is Negatively Associated with Chlamydial Disease in the Koala.

    abstract::Koalas (Phascolarctos cinereus) are native Australian marsupials whose populations are in decline from a range of threats. Infectious diseases caused by the bacterium Chlamydia pecorum and other pathogens are of particular concern. We analysed 26 poly-A selected RNA-sequencing libraries from a data set designed to stu...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v11030211

    authors: Harvey E,Madden D,Polkinghorne A,Holmes EC

    更新日期:2019-03-02 00:00:00

  • PCR Detection and Phylogenetic Analysis of Megalocytivirus Isolates in Farmed Giant Sea Perch Lates calcarifer in Southern Taiwan.

    abstract::The Megalocytivirus genus includes three genotypes, red sea bream iridovirus (RSIV), infectious spleen and kidney necrosis virus (ISKNV), and turbot reddish body iridovirus (TRBIV), and has caused mass mortalities in various marine and freshwater fish species in East and Southeast Asia. Of the three genotypes, TRBIV-l...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v12060681

    authors: Tsai JM,Huang SL,Yang CD

    更新日期:2020-06-24 00:00:00

  • Arbidol (Umifenovir): A Broad-Spectrum Antiviral Drug That Inhibits Medically Important Arthropod-Borne Flaviviruses.

    abstract::Arthropod-borne flaviviruses are human pathogens of global medical importance, against which no effective small molecule-based antiviral therapy has currently been reported. Arbidol (umifenovir) is a broad-spectrum antiviral compound approved in Russia and China for prophylaxis and treatment of influenza. This compoun...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v10040184

    authors: Haviernik J,Štefánik M,Fojtíková M,Kali S,Tordo N,Rudolf I,Hubálek Z,Eyer L,Ruzek D

    更新日期:2018-04-10 00:00:00

  • Middle East Respiratory Syndrome Vaccine Candidates: Cautious Optimism.

    abstract::Efforts towards developing a vaccine for Middle East respiratory syndrome coronavirus (MERS-CoV) have yielded promising results. Utilizing a variety of platforms, several vaccine approaches have shown efficacy in animal models and begun to enter clinical trials. In this review, we summarize the current progress toward...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v11010074

    authors: Schindewolf C,Menachery VD

    更新日期:2019-01-17 00:00:00

  • Orchestrating the Selection and Packaging of Genomic RNA by Retroviruses: An Ensemble of Viral and Host Factors.

    abstract::Infectious retrovirus particles contain two copies of unspliced viral RNA that serve as the viral genome. Unspliced retroviral RNA is transcribed in the nucleus by the host RNA polymerase II and has three potential fates: (1) it can be spliced into subgenomic messenger RNAs (mRNAs) for the translation of viral protein...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v8090257

    authors: Kaddis Maldonado RJ,Parent LJ

    更新日期:2016-09-20 00:00:00

  • Temperature Sensitive Mutations in Influenza A Viral Ribonucleoprotein Complex Responsible for the Attenuation of the Live Attenuated Influenza Vaccine.

    abstract::Live attenuated influenza vaccines (LAIV) have prevented morbidity and mortality associated with influenza viral infections for many years and represent the best therapeutic option to protect against influenza viral infections in humans. However, the development of LAIV has traditionally relied on empirical methods, s...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v10100560

    authors: Martínez-Sobrido L,Peersen O,Nogales A

    更新日期:2018-10-15 00:00:00

  • Development of hematopoietic stem cell based gene therapy for HIV-1 infection: considerations for proof of concept studies and translation to standard medical practice.

    abstract::Over the past 15 years we have been investigating an alternative approach to treating HIV-1/AIDS, based on the creation of a disease-resistant immune system through transplantation of autologous, gene-modified (HIV-1-resistant) hematopoietic stem and progenitor cells (GM-HSPC). We propose that the expression of select...

    journal_title:Viruses

    pub_type: 临床试验,杂志文章

    doi:10.3390/v5112898

    authors: DiGiusto DL,Stan R,Krishnan A,Li H,Rossi JJ,Zaia JA

    更新日期:2013-11-22 00:00:00

  • NCBI's Virus Discovery Codeathon: Building "FIVE" -The Federated Index of Viral Experiments API Index.

    abstract::Viruses represent important test cases for data federation due to their genome size and the rapid increase in sequence data in publicly available databases. However, some consequences of previously decentralized (unfederated) data are lack of consensus or comparisons between feature annotations. Unifying or displaying...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v12121424

    authors: Martí-Carreras J,Gener AR,Miller SD,Brito AF,Camacho CE,Connor R,Deboutte W,Glickman C,Kristensen DM,Meyer WK,Modha S,Norris AL,Saha S,Belford AK,Biederstedt E,Brister JR,Buchmann JP,Cooley NP,Edwards RA,Javkar K,

    更新日期:2020-12-10 00:00:00

  • Base composition and translational selection are insufficient to explain codon usage bias in plant viruses.

    abstract::Viral codon usage bias may be the product of a number of synergistic or antagonistic factors, including genomic nucleotide composition, translational selection, genomic architecture, and mutational or repair biases. Most studies of viral codon bias evaluate only the relative importance of genomic base composition and ...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v5010162

    authors: Cardinale DJ,DeRosa K,Duffy S

    更新日期:2013-01-15 00:00:00

  • A Nanoparticle-Based Trivalent Vaccine Targeting the Glycan Binding VP8* Domains of Rotaviruses.

    abstract::Rotavirus causes severe gastroenteritis in children. Although vaccines are implemented, rotavirus-related diarrhea still claims ~200,000 lives annually worldwide, mainly in low-income settings, pointing to a need for improved vaccine tactics. To meet such a public health need, a P24-VP8* nanoparticle displaying the gl...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v13010072

    authors: Xia M,Huang P,Jiang X,Tan M

    更新日期:2021-01-06 00:00:00

  • The Response of Heterotrophic Prokaryote and Viral Communities to Labile Organic Carbon Inputs Is Controlled by the Predator Food Chain Structure.

    abstract::Factors controlling the community composition of marine heterotrophic prokaryotes include organic-C, mineral nutrients, predation, and viral lysis. Two mesocosm experiments, performed at an Arctic location and bottom-up manipulated with organic-C, had very different results in community composition for both prokaryote...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v9090238

    authors: Sandaa RA,Pree B,Larsen A,Våge S,Töpper B,Töpper JP,Thyrhaug R,Thingstad TF

    更新日期:2017-08-23 00:00:00

  • Modes of Human T Cell Leukemia Virus Type 1 Transmission, Replication and Persistence.

    abstract::Human T-cell leukemia virus type 1 (HTLV-1) is a retrovirus that causes cancer (Adult T cell Leukemia, ATL) and a spectrum of inflammatory diseases (mainly HTLV-associated myelopathy-tropical spastic paraparesis, HAM/TSP). Since virions are particularly unstable, HTLV-1 transmission primarily occurs by transfer of a c...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v7072793

    authors: Carpentier A,Barez PY,Hamaidia M,Gazon H,de Brogniez A,Perike S,Gillet N,Willems L

    更新日期:2015-07-07 00:00:00

  • n-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR-pMHC Interaction of Anti-Viral CD8+ T Cells.

    abstract::The immune-suppressive effects of omega-3 (n-3) polyunsaturated fatty acids (PUFAs) on T cells have been observed via multiple in vitro and in vivo models. However, the precise mechanism that causes these effects is still undefined. In this study, we investigated whether n-3 PUFAs regulated T cell receptor (TCR) and p...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v12060639

    authors: Lim Y,Kim S,Kim S,Kim DI,Kang KW,Hong SH,Lee SM,Koh HR,Seo YJ

    更新日期:2020-06-12 00:00:00

  • Poxviruses in bats … so what?

    abstract::Poxviruses are important pathogens of man and numerous domestic and wild animal species. Cross species (including zoonotic) poxvirus infections can have drastic consequences for the recipient host. Bats are a diverse order of mammals known to carry lethal viral zoonoses such as Rabies, Hendra, Nipah, and SARS. Consequ...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v6041564

    authors: Baker KS,Murcia PR

    更新日期:2014-04-03 00:00:00

  • High Throughput Screening of FDA-Approved Drug Library Reveals the Compounds that Promote IRF3-Mediated Pro-Apoptotic Pathway Inhibit Virus Replication.

    abstract::Interferon (IFN) regulatory factor 3 (IRF3) is the key transcription factor for the induction of IFN and antiviral genes. The absence of antiviral genes in IRF3 deficiency leads to susceptibility to a wide range of viral infections. Previously, we uncovered a function for nontranscriptional IRF3 (nt-IRF3), RLR (RIG-I-...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v12040442

    authors: Glanz A,Chawla K,Fabry S,Subramanian G,Garcia J,Jay B,Ciricillo J,Chakravarti R,Taylor RT,Chattopadhyay S

    更新日期:2020-04-14 00:00:00

  • Complete Genome Sequence of Germline Chromosomally Integrated Human Herpesvirus 6A and Analyses Integration Sites Define a New Human Endogenous Virus with Potential to Reactivate as an Emerging Infection.

    abstract::Human herpesvirus-6A and B (HHV-6A, HHV-6B) have recently defined endogenous genomes, resulting from integration into the germline: chromosomally-integrated "CiHHV-6A/B". These affect approximately 1.0% of human populations, giving potential for virus gene expression in every cell. We previously showed that CiHHV-6A w...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v8010019

    authors: Tweedy J,Spyrou MA,Pearson M,Lassner D,Kuhl U,Gompels UA

    更新日期:2016-01-15 00:00:00

  • Equine Parvovirus-Hepatitis Frequently Detectable in Commercial Equine Serum Pools.

    abstract::An equine parvovirus-hepatitis (EqPV-H) has been recently identified in association with equine serum hepatitis, also known as Theiler's disease. This disease was first described by Arnold Theiler in 1918 and is often observed after applications with blood products in equines. So far, the virus has only been described...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v11050461

    authors: Meister TL,Tegtmeyer B,Postel A,Cavalleri JV,Todt D,Stang A,Steinmann E

    更新日期:2019-05-21 00:00:00

  • Recombinant Pseudorabies Virus (PRV) Expressing Firefly Luciferase Effectively Screened for CRISPR/Cas9 Single Guide RNAs and Antiviral Compounds.

    abstract::A Pseudorabies virus (PRV) variant has emerged in China since 2011 that is not protected by commercial vaccines, and has not been well studied. The PRV genome is large and difficult to manipulate, but it is feasible to use clustered, regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology. However, id...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v8040090

    authors: Tang YD,Liu JT,Fang QQ,Wang TY,Sun MX,An TQ,Tian ZJ,Cai XH

    更新日期:2016-03-29 00:00:00

  • Bacteriophage Applications for Food Production and Processing.

    abstract::Foodborne illnesses remain a major cause of hospitalization and death worldwide despite many advances in food sanitation techniques and pathogen surveillance. Traditional antimicrobial methods, such as pasteurization, high pressure processing, irradiation, and chemical disinfectants are capable of reducing microbial p...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v10040205

    authors: Moye ZD,Woolston J,Sulakvelidze A

    更新日期:2018-04-19 00:00:00

  • Rodent Papillomaviruses.

    abstract::Preclinical infection model systems are extremely valuable tools to aid in our understanding of Human Papillomavirus (HPV) biology, disease progression, prevention, and treatments. In this context, rodent papillomaviruses and their respective infection models are useful tools but remain underutilized resources in the ...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v9120362

    authors: Uberoi A,Lambert PF

    更新日期:2017-11-27 00:00:00

  • Suppression of Type I Interferon Signaling by Flavivirus NS5.

    abstract::Type I interferon (IFN-I) is the first line of mammalian host defense against viral infection. To counteract this, the flaviviruses, like other viruses, have encoded a variety of antagonists, and use a multi-layered molecular defense strategy to establish their infections. Among the most potent antagonists is non-stru...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v10120712

    authors: Thurmond S,Wang B,Song J,Hai R

    更新日期:2018-12-14 00:00:00

  • The molecular biology of feline immunodeficiency virus (FIV).

    abstract::Feline immunodeficiency virus (FIV) is widespread in feline populations and causes an AIDS-like illness in domestic cats. It is highly prevalent in several endangered feline species. In domestic cats FIV infection is a valuable small animal model for HIV infection. In recent years there has been sa significant increas...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v3112192

    authors: Kenyon JC,Lever AM

    更新日期:2011-11-01 00:00:00