Abstract:
BACKGROUND:Prostate cancer is highly prevalent worldwide. Androgen deprivation therapy (ADT) remains the treatment of choice for incurable prostate cancer, but majority of patients develop disease recurrence following ADT. There is therefore an urgent need for early detection of treatment resistance. METHODS:Isogenic androgen-responsive (CWR22Res) and castration-resistant (22Rv1) human prostate cancer cells were implanted into the anterior lobes of the prostate in CD-1 Nu mice to generate prostate orthografts. Castrated mice bearing CWR22Res and 22Rv1 orthografts mimic clinical prostate cancer following acute and chronic ADT, respectively. 18F-Fluciclovine (1-amino-3-fluorocyclobutane-1-carboxylic acid) with a radiochemical purity of > 99% was produced on a FASTlab synthesiser. Ki67 staining in endpoint orthografts was studied. Western blot, quantitative RT-PCR and next-generation sequencing transcriptomic analyses were performed to assess the expression levels of amino acid transporters (including LAT1 and ASCT2, which have been implicated for Fluciclovine uptake). Longitudinal metabolic imaging with 18F-Fluciclovine-based positron emission tomography (PET) was performed to study tumour response following acute and chronic ADT. RESULTS:Both immunohistochemistry analysis of endpoint prostate tumours and longitudinal 18F-Fluciclovine imaging revealed tumour heterogeneity, particularly following ADT, with in vivo 18F-Fluciclovine uptake correlating to viable cancer cells in both androgen-proficient and castrated environment. Highlighting tumour subpopulation following ADT, both SUVpeak and coefficient of variation (CoV) values of 18F-Fluciclovine uptake are consistent with tumour heterogeneity revealed by immunohistochemistry. We studied the expression of amino acid transporters (AATs) for 18F-Fluciclovine, namely LAT1 (SLC7A5 and SLC3A2) and ASCT2 (SLC1A5). SLC7A5 and SLC3A2 were expressed at relatively high levels in 22Rv1 castration-resistant orthografts following chronic ADT (modelling clinical castration-resistant disease), while SLC1A5 was preferentially expression in CWR22Res tumours following acute ADT. Additional AATs such as SLC43A2 (LAT4) were shown to be upregulated following chronic ADT by transcriptomic analysis; their role in Fluciclovine uptake warrants investigation. CONCLUSION:We studied in vivo 18F-Fluciclovine uptake in human prostate cancer orthograft models following acute and chronic ADT. 18F-Fluciclovine uptakes highlight tumour heterogeneity that may explain castration resistance and can be exploited as a clinical biomarker.
journal_name
EJNMMI Resjournal_title
EJNMMI researchauthors
Malviya G,Patel R,Salji M,Martinez RS,Repiscak P,Mui E,Champion S,Mrowinska A,Johnson E,AlRasheedi M,Pimlott S,Lewis D,Leung HYdoi
10.1186/s13550-020-00728-9subject
Has Abstractpub_date
2020-11-25 00:00:00pages
143issue
1issn
2191-219Xpii
10.1186/s13550-020-00728-9journal_volume
10pub_type
杂志文章相关文献
EJNMMI Research文献大全abstract:BACKGROUND:18F-FDG-PET imaging has emerged as a promising method in the diagnosis of chronic osteomyelitis commonly due to Staphylococcus aureus. The inaccuracy of 18 F-FDG-PET in the detection of periprosthetic joint infections may be related to the predominance of low-virulent S. epidermidis strains as the causative ...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/2191-219X-2-41
更新日期:2012-07-23 00:00:00
abstract::An amendment to this paper has been published and can be accessed via the original article. ...
journal_title:EJNMMI research
pub_type: 已发布勘误
doi:10.1186/s13550-020-00719-w
更新日期:2020-10-27 00:00:00
abstract:PURPOSE:Pathological complete response to the neoadjuvant therapy (NAT) for triple negative breast cancer (TNBC) is predictive of prolonged patient survival. Methods for early evaluation of NAT efficiency are still needed, in order to rapidly adjust the therapeutic strategy in case of initial non-response. One option f...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-019-0587-5
更新日期:2020-01-06 00:00:00
abstract:BACKGROUND:Non-small cell lung carcinoma (NSCLC) represents approximately 80% of lung cancer cases, and over 60% of these tumors express the epidermal growth factor receptor (EGFR). Activating mutations in the tyrosine kinase (TK) domain of the EGFR are detected in 10% to 30% of NSCLC patients, and evidence of their pr...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-014-0080-0
更新日期:2015-02-12 00:00:00
abstract:BACKGROUND:The positron emission tomography (PET) ligand 68Ga-Glu-urea-Lys(Ahx)-HBED-CC (68Ga-PSMA-11) targets the prostate-specific membrane antigen (PSMA), upregulated in prostate cancer cells. Although 68Ga-PSMA-11 PET is widely used in research and clinical practice, full kinetic modeling has not yet been reported ...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-020-0594-6
更新日期:2020-02-24 00:00:00
abstract:BACKGROUND:In this work, the in vitro and in vivo stabilities and the pharmacology of HPMA-made homopolymers were studied by means of radiometal-labeled derivatives. Aiming to identify the fewer amount and the optimal DOTA-linker structure that provides quantitative labeling yields, diverse DOTA-linker systems were con...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-018-0372-x
更新日期:2018-02-27 00:00:00
abstract:BACKGROUND:We recently showed improved between-subject variability in our [18F]fluorodeoxyglucose positron emission tomography (FDG-PET) experiments using a Michaelis-Menten transport model to calculate the metabolic tumor glucose uptake rate extrapolated to the hypothetical condition of glucose saturation: MRgl...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/2191-219X-2-35
更新日期:2012-06-27 00:00:00
abstract:BACKGROUND:In vivo determination of regional pulmonary blood flow (PBF) is a valuable tool for the evaluation of many lung diseases. In this study, the use of 68Ga-DOTA PET for the in vivo quantitative determination of regional PBF is proposed. This methodology was implemented and tested in healthy pigs and validated u...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-017-0259-2
更新日期:2017-12-01 00:00:00
abstract:BACKGROUND:Glioblastoma, the most frequent and aggressive of all astrocytomas, presents a clear predominance in male humans, but the assessment of sexual differences in its tumourigenesis and growth has received little attention so far. In this study, we aim to identify gender-dependent surrogate markers in an animal m...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-014-0044-4
更新日期:2014-12-01 00:00:00
abstract:BACKGROUND:To combine the sensitivity of bioluminescent imaging (BLI) with the 3D and quantitative properties of pinhole single-photon emission computed tomography (SPECT)/micro-computed tomography (CT) (phSPECT/micro-CT), we generated stable cell lines that express a yellow-fluorescent protein (YFP) and Gaussia lucife...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-014-0032-8
更新日期:2014-06-26 00:00:00
abstract:BACKGROUND:[18 F]fluorodopa (FDOPA) positron emission tomography (PET) allows assessment of levodopa (LDOPA) metabolism and is widely used to study Parkinson's disease. We examined how [18 F]FDOPA PET-derived kinetic parameters relate the dopamine (DA) and DA metabolite content of extracellular fluid measured by microd...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/2191-219X-3-69
更新日期:2013-10-02 00:00:00
abstract:BACKGROUND:Differentiated thyroid carcinoma (DTC) is the most common type of thyroid cancer. Treatment with surgery, radioactive iodine (RAI), and TSH suppression is effective in most patients. Five to 15% of patients become RAI refractory and need alternative therapy; however, treatment options are limited. 68Ga-PSMA ...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-020-0610-x
更新日期:2020-03-06 00:00:00
abstract:BACKGROUND:We aimed to determine the accuracy of laser Doppler perfusion imaging (LDPI) in an animal model for hind limb ischemia. METHODS:We used a murine (C57Bl/6 mice) ischemic hind limb model in which we compared LDPI with the clinically used (99m)Tc-sestamibi SPECT perfusion imaging (n = 7). In addition, we used ...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-016-0199-2
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND:Off-target binding in the choroid plexus (CP) may cause spill-in of the tau PET tracer [18F] flortaucipir into the adjacent hippocampus region. The impact of this spill-in on hippocampal uptake was assessed using a novel partial volume correction method (PVC). METHODS:PVC was performed on 20 [18F] flortauci...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-018-0432-2
更新日期:2018-08-15 00:00:00
abstract:BACKGROUND:PET/MRI can be used for the detection of disease in biochemical recurrence (BCR) patients imaged with 68Ga-PSMA-11 PET. This study was designed to determine the optimal MRI sequences to localize positive findings on 68Ga-PSMA-11 PET of patients with BCR after definitive therapy. Fifty-five consecutive prosta...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-017-0327-7
更新日期:2017-09-19 00:00:00
abstract:BACKGROUND:Routine work-up for transarterial radioembolization, based on clinical and laboratory parameters, sometimes fails, resulting in severe hepatotoxicity in up to 5% of patients. Quantitative assessment of the pretreatment liver function and its segmental distribution, using hepatobiliary scintigraphy may improv...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-016-0248-x
更新日期:2017-12-01 00:00:00
abstract:PURPOSE:We have developed a multi-modal imaging approach for SIRT, combining 99mTc-MAA SPECT/CT and/or 90Y PET, 18F-FDG PET/CT, and contrast-enhanced CBCT for voxel-based dosimetry, as a tool for treatment planning and verification. For radiation dose prediction calculations, a segmentation of the total liver volume an...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-019-0485-x
更新日期:2019-02-20 00:00:00
abstract:BACKGROUND:Hypoxia within solid tumors confers radiation resistance and a poorer prognosis. 124I-iodoazomycin galactopyranoside (124I-IAZGP) has shown promise as a hypoxia radiotracer in animal models. We performed a clinical study to evaluate the safety, biodistribution, and imaging characteristics of 124I-IAZGP in pa...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/2191-219X-3-42
更新日期:2013-06-03 00:00:00
abstract:BACKGROUND:Yoga is increasingly popular worldwide with several physical and mental benefits, but the underlying neurobiology remains unclear. Whereas many studies have focused on pure meditational aspects, the triad of yoga includes meditation, postures, and breathing. We conducted a cross-sectional study comparing exp...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-020-00636-y
更新日期:2020-05-14 00:00:00
abstract:UNLABELLED: BACKGROUND:Iodine-123-β-CIT, a single-photon emission computed tomography (SPECT) ligand for dopamine transporters (DATs), has been used for in vivo studies in humans, monkeys, and rats but has not yet been used extensively in mice. To validate the imaging and analysis methods for preclinical DAT imaging, ...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/2191-219X-2-55
更新日期:2012-09-29 00:00:00
abstract:BACKGROUND:Small-cell lung carcinoma (SCLC) is an aggressive malignancy characterised by an early relapse, a tendency towards drug resistance, and a high incidence of metastasis. SCLC cells are of neuroendocrine origin and express high levels of somatostatin receptors; therefore, future treatment might involve targetin...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/2191-219X-1-19
更新日期:2011-08-26 00:00:00
abstract:BACKGROUND:New therapies to treat diabetic peripheral artery disease (PAD) require target-specific non-invasive imaging modalities to follow efficacy. As a translational study, we performed targeted imaging of receptors for vascular endothelial growth factor (VEGF) in response to anterior femoral artery occlusion (FAO)...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-020-00626-0
更新日期:2020-05-12 00:00:00
abstract:BACKGROUND:The biology of the vulnerable plaque includes increased inflammation and rapid growth of vasa vasorum, processes that are associated with enhanced vascular endothelial growth factor (VEGF)/ imaging receptors for VEGF (VEGFR) signaling and are accelerated in diabetes. This study was designed to test the hypot...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-014-0041-7
更新日期:2014-12-01 00:00:00
abstract:BACKGROUND:Given the increasing clinical use of PET/MRI, potential risks to patients from simultaneous exposure to ionising radiation and (electro)magnetic fields should be thoroughly investigated as a precaution. With this aim, the genotoxic potential of 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) and a strong static m...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-020-00625-1
更新日期:2020-04-28 00:00:00
abstract:PURPOSE:To investigate the added value and diagnostic accuracy of 68Ga-PSMA PET/CT versus bone scintigraphy (BS) for bone metastasis detection at the primary staging of prostate cancer (PCa). METHODS:Inclusion criteria involved consecutive patients with newly diagnosed intermediate- to high-risk PCa, who had undergone...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-020-00618-0
更新日期:2020-04-08 00:00:00
abstract:BACKGROUND:Two novel methods of image reconstruction, xSPECT Quant (xQ) and xSPECT Bone (xB), that use an ordered subset conjugate gradient minimizer (OSCGM) for SPECT/CT reconstruction have been proposed. The present study compares the performance characteristics of xQ, xB, and conventional Flash3D (F3D) reconstructio...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-020-00659-5
更新日期:2020-06-29 00:00:00
abstract::Translocator protein (TSPO) is a biomarker for detecting neuroinflammation by PET. 11C-(R)-PK11195 has been used to image TSPO since the 1980s. Here, we compared the utility of four 11C-labeled ligands-(R)-PK11195, PBR28, DPA-713, and ER176-to quantify TSPO in healthy humans. For all of these ligands, BP ND (specific-...
journal_title:EJNMMI research
pub_type: 信件
doi:10.1186/s13550-017-0334-8
更新日期:2017-10-16 00:00:00
abstract:PURPOSE:Chimeric antibody Miltuximab®, a human IgG1 engineered from the parent antibody MIL-38, is in clinical development for solid tumour therapy. Miltuximab® targets glypican-1 (GPC-1), a cell surface protein involved in tumour growth, which is overexpressed in solid tumours, including prostate cancer (PCa). This st...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-020-00637-x
更新日期:2020-05-07 00:00:00
abstract:BACKGROUND:In the present study, we used multimodal imaging to investigate biodistribution in rats after intravenous administration of a new 99mTc-labeled delivery system consisting of polymer-shelled microbubbles (MBs) functionalized with diethylenetriaminepentaacetic acid (DTPA), thiolated poly(methacrylic acid) (PMA...
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/2191-219X-3-12
更新日期:2013-02-25 00:00:00
abstract:BACKGROUND:We investigated the association between physiologic bowel FDG uptake and gut microbiota. FDG uptake in the normal large and small intestine is widely variable both in distribution and intensity. The etiology of physiologic bowel 18F-FDG activity remains unknown. RESULTS:We included 63 healthy male subjects....
journal_title:EJNMMI research
pub_type: 杂志文章
doi:10.1186/s13550-017-0318-8
更新日期:2017-08-31 00:00:00