Abstract:
BACKGROUND:Among manufactured or engineered nanoparticles, carbon black (CB) has largest production worldwide and is also an occupational respiratory hazard commonly seen in rubber industry. Few studies have assessed the risk for cardiovascular disease in carbon black exposed populations. An endothelial biosensor assay was used to quantify the capacity of sera from 82 carbon black packers (CBP) and 106 non-CBPs to induce endothelial cell activation ex vivo. The mediation effect of circulatory proinflammatory factors on the association between carbon black exposure and endothelial cell activation was assessed and further validated using in vitro intervention experiments. RESULTS:The average elemental carbon level inside carbon black bagging facilities was 657.0 μg/m3, which was 164-fold higher than that seen in reference areas (4.0 μg/m3). A global index was extracted from mRNA expression of seven candidate biosensor genes using principal component analysis and used to quantify the magnitude of endothelial cell activation. This global index was found to be significantly altered in CBPs compared to non-CBPs (P < 0.0001), however this difference did not vary by smoking status (P = 0.74). Individual gene analyses identified that de novo expression of key adhesion molecules (e.g., ICAM and VCAM) and chemotactic factors (e.g., CCL2, CCL5, and CXCL8) responsible for the recruitment of leukocytes was dramatically induced in CBPs with CXCL8 showing the highest fold of induction (relative quantification = 9.1, P < 0.0001). The combination of mediation analyses and in vitro functional validation confirmed TNF-α, IL-1β, and IL-6 as important circulatory factors mediating the effects of carbon black exposure on endothelial cell activation responses. CONCLUSIONS:Inflammatory mediators in sera from CBPs may bridge carbon black exposure and endothelial cell activation response assessed ex vivo. CBPs may have elevated risk for cardiovascular diseases when comorbidity exists. Our study may serve as a benchmark for understanding health effects of engineered carbon based nanoparticles with environmental and occupational health relevance.
journal_name
Part Fibre Toxicoljournal_title
Particle and fibre toxicologyauthors
Tang J,Cheng W,Gao J,Li Y,Yao R,Rothman N,Lan Q,Campen MJ,Zheng Y,Leng Sdoi
10.1186/s12989-020-00378-8subject
Has Abstractpub_date
2020-09-29 00:00:00pages
47issue
1issn
1743-8977pii
10.1186/s12989-020-00378-8journal_volume
17pub_type
杂志文章abstract:UNLABELLED:A novel methodology to detect unlabeled inorganic nanoparticles was experimentally demonstrated using a mixture of nano-sized (70 nm) and submicron (250 nm) silicon dioxide particles added to mammalian tissue. The size and concentration of environmentally relevant inorganic particles in a tissue sample can b...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-5-18
更新日期:2008-12-03 00:00:00
abstract:BACKGROUND:Inhalation exposure studies of mice were conducted to determine if multi-walled carbon nanotubes (MWCNT) distribute to the tracheobronchial lymphatics, parietal pleura, respiratory musculature and/or extrapulmonary organs. Male C57BL/6 J mice were exposed in a whole-body inhalation system to a 5 mg/m3 MWCNT ...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-38
更新日期:2013-08-09 00:00:00
abstract:BACKGROUND:Fine particulate matter (PM2.5) related mild inflammation, altered autonomic control of cardiovascular function, and changes to cell function have been observed in controlled human exposure studies. METHODS:To measure the systemic and cardiopulmonary impacts of low-level PM exposure, we exposed 20 healthy, ...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-020-00389-5
更新日期:2020-11-16 00:00:00
abstract:BACKGROUND:Combustion generated particulate matter is deposited in the respiratory tract and pose a hazard to the lungs through their potential to cause oxidative stress and inflammation. We have previously shown that combustion of fuels and chlorinated hydrocarbons produce semiquinone-type radicals that are stabilized...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-6-11
更新日期:2009-04-17 00:00:00
abstract:BACKGROUND:Nanoparticle pharmacokinetics and biological effects are influenced by several factors. We assessed the effects of amorphous SiO2 coating on the pharmacokinetics of zinc oxide nanoparticles (ZnO NPs) following intratracheal (IT) instillation and gavage in rats. METHODS:Uncoated and SiO2-coated ZnO NPs were ...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-014-0044-6
更新日期:2014-09-03 00:00:00
abstract:BACKGROUND:Aluminum oxide-based nanowhiskers (AO nanowhiskers) have been used in manufacturing processes as catalyst supports, flame retardants, adsorbents, or in ceramic, metal and plastic composite materials. They are classified as high aspect ratio nanomaterials. Our aim was to assess in vivo toxicity of inhaled AO ...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-9-22
更新日期:2012-06-19 00:00:00
abstract:BACKGROUND:The exact role of primary nanoparticle (NP) size and their degree of agglomeration in aerosols on the determination of pulmonary effects is still poorly understood. Smaller NP are thought to have greater biological reactivity, but their level of agglomeration in an aerosol may also have an impact on pulmonar...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-48
更新日期:2013-10-04 00:00:00
abstract:BACKGROUND:Epidemiologic and animal studies have shown that particulate air pollution is associated with increased risk of lung and cardiovascular diseases. Although the exact mechanisms by which particles induce cardiovascular diseases are not known, studies suggest involvement of systemic acute phase responses, inclu...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-6-12
更新日期:2009-04-20 00:00:00
abstract:BACKGROUND:Inhalative nanocarriers for local or systemic therapy are promising. Gold nanoparticles (AuNP) have been widely considered as candidate material. Knowledge about their interaction with the lungs is required, foremost their uptake by surface macrophages and epithelial cells. METHODS:Scnn1b-Tg and Wt mice inh...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-19
更新日期:2013-05-16 00:00:00
abstract:OBJECTIVE:The development of nanotechnology has spurred concerns about the health effects of exposure to nanoparticles (NPs) and ultrafine particles (UFPs). Toxicological data on NPs and UFPs may provide evidence to support the development of regulations to reduce the risk of particle exposure. We tried to provide fund...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-015-0082-8
更新日期:2015-03-19 00:00:00
abstract:BACKGROUND:The alveolar macrophage (AM) - first line of innate immune defence against pathogens and environmental irritants - constitutively expresses peroxisome-proliferator activated receptor γ (PPARγ). PPARγ ligand-induced activation keeps the AM quiescent, and thereby contributes to combat invaders and resolve infl...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-8-28
更新日期:2011-09-20 00:00:00
abstract:BACKGROUND:In myeloid cells the inflammasome plays a crucial role in innate immune defenses against pathogen- and danger-associated patterns such as crystalline silica. Respirable mineral particles impinge upon the lung epithelium causing irreversible damage, sustained inflammation and silicosis. In this study we inves...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-3
更新日期:2013-02-12 00:00:00
abstract:BACKGROUND:Epidemiological studies suggest that prenatal and early life environmental exposures have adverse effects on pulmonary function and are important contributors in the development of childhood asthma and allergic disease. The mechanism by which environmental tobacco smoke (ETS) exposure in utero promotes the d...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-017-0212-6
更新日期:2017-08-22 00:00:00
abstract:BACKGROUND:Although biocides at low concentrations have been used to control pests, they can be more harmful than industrial chemicals as humans are directly and frequently exposed to such biocides. Benzalkonium chloride (BAC or BKC) is a non-toxic substance used to control pests. Recently, BAC has been increasingly us...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-020-0339-8
更新日期:2020-01-28 00:00:00
abstract::In their Commentary Saber et al. (Part Fibre Toxicol 16: 44, 2019) argue that chronic inhalation studies in rats can be used for assessing the lung cancer risk of insoluble nanomaterials. The authors make several significant errors in their interpretation and representation of the underlying science. In this Letter to...
journal_title:Particle and fibre toxicology
pub_type: 评论,信件
doi:10.1186/s12989-020-00365-z
更新日期:2020-07-16 00:00:00
abstract:BACKGROUND:There is a great need for screening tools capable of rapidly assessing nanomaterial toxicity. One impediment to the development of reliable in vitro screening methods is the need for accurate measures of cellular dose. We present here a methodology that enables accurate determination of delivered to cell dos...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-11-20
更新日期:2014-05-01 00:00:00
abstract::Particulate matter (PM) pollution is responsible for hundreds of thousands of deaths worldwide, the majority due to cardiovascular disease (CVD). While many potential pathophysiological mechanisms have been proposed, there is not yet a consensus as to which are most important in causing pollution-related morbidity/mor...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章,评审
doi:10.1186/1743-8977-9-21
更新日期:2012-06-19 00:00:00
abstract::The uncertainty regarding the effects and risks of nanomaterials on human health and the environment, and how they should be tested and assessed in the context of current regulations, is clearly holding back the full exploitation of the innovative potential of nanomaterials. To reduce this uncertainty, the European Un...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-017-0235-z
更新日期:2017-12-19 00:00:00
abstract:BACKGROUND:Inhalation of environmental (nano) particles (NP) as well as persistent herpesvirus-infection are potentially associated with chronic lung disease and as both are omnipresent in human society a coincidence of these two factors is highly likely. We hypothesized that NP-exposure of persistently herpesvirus-inf...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-016-0181-1
更新日期:2017-01-10 00:00:00
abstract:BACKGROUND:Mitochondria are both a sensitive target and a primary source of oxidative stress, a key pathway of air particulate matter (PM)-associated diseases. Mitochondrial DNA copy number (MtDNAcn) is a marker of mitochondrial damage and malfunctioning. We evaluated whether ambient PM exposure affects MtDNAcn in a hi...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-17
更新日期:2013-04-29 00:00:00
abstract:BACKGROUND:Pleural fibrosis and malignant mesotheliomas (MM) occur after exposures to pathogenic fibers, yet the mechanisms initiating these diseases are unclear. RESULTS:We document priming and activation of the NLRP3 inflammasome in human mesothelial cells by asbestos and erionite that is causally related to release...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-39
更新日期:2013-08-13 00:00:00
abstract:BACKGROUND:Inhaled nanoparticles have been reported in some instances to translocate from the nostril to the olfactory bulb in exposed rats. In close proximity to the olfactory bulb is the olfactory mucosa, within which resides a niche of multipotent cells. Cells isolated from this area may provide a relevant in vitro ...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-54
更新日期:2013-10-21 00:00:00
abstract:BACKGROUND:Exposure to small size particulate matter in urban air is regarded as a risk factor for cardiovascular effects, whereas there is little information about the impact on the cardiovascular system by exposure to pure carbonaceous materials in the nano-size range. C60 fullerenes are nano-sized particles that are...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-6-5
更新日期:2009-02-25 00:00:00
abstract:BACKGROUND:The etiology and progression of neurodegenerative disorders depends on the interactions between a variety of factors including: aging, environmental exposures, and genetic susceptibility factors. Enhancement of proinflammatory events appears to be a common link in different neurological impairments, includin...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-7-12
更新日期:2010-05-17 00:00:00
abstract:BACKGROUND:Carbon nanotubes and nanofibers (CNT/F) have known toxicity but simultaneous comparative studies of the broad material class, especially those with a larger diameter, with computational analyses linking toxicity to their fundamental material characteristics was lacking. It was unclear if all CNT/F confer sim...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-020-00392-w
更新日期:2020-12-07 00:00:00
abstract:BACKGROUND:Traffic-generated air pollution-exposure is associated with adverse effects in the central nervous system (CNS) in both human exposures and animal models, including neuroinflammation and neurodegeneration. While alterations in the blood brain barrier (BBB) have been implicated as a potential mechanism of air...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-62
更新日期:2013-12-17 00:00:00
abstract:INTRODUCTION:Exposure to air pollution is associated with increased cardiovascular morbidity, although the underlying mechanisms are unclear. Vascular dysfunction reduces arterial compliance and increases central arterial pressure and left ventricular after-load. We determined the effect of diesel exhaust exposure on a...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-6-7
更新日期:2009-03-13 00:00:00
abstract:BACKGROUND:Carbon nanotubes (CNTs) are being increasingly industrialized and applied for various products. As of today, although several toxicological evaluations of CNTs have been conducted, designing safer CNTs is not practiced because reaction kinetics of CNTs with bioactive species is not fully understood. RESULTS...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-44
更新日期:2013-09-02 00:00:00
abstract:: Air Pollution has been associated with significant adverse health effects leading to increased morbidity and mortality. Cumulative epidemiological and experimental data have shown that exposure to air pollutants lead to increased cardiovascular ischemic events and enhanced atherosclerosis. It appears that these assoc...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-6-24
更新日期:2009-09-18 00:00:00
abstract:BACKGROUND:Silica continues to represent an intriguing topic of fundamental and applied research across various scientific fields, from geology to physics, chemistry, cell biology, and particle toxicology. The pathogenic activity of silica is variable, depending on the physico-chemical features of the particles. In the...
journal_title:Particle and fibre toxicology
pub_type: 信件
doi:10.1186/s12989-019-0315-3
更新日期:2019-08-16 00:00:00