Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applications.


:Antioxidant compounds from cyanobacteria may constitute a natural alternative to current synthetic antioxidants, which contain preservatives and suspected toxicity. In this work, we evaluate the antioxidant potential of cyanobacterial strains of distinct species/genus isolated from freshwater (n = 6), soil (n = 1) and wastewater (n = 1) environments. Lyophilized biomass obtained from in-vitro cultures of those strains was extracted with ethanol and methanol. The antioxidant potential was evaluated by chemical (DPPH scavenging method, β-carotene bleaching assay, determination of total phenolic and total flavonoid compounds) and biological (H2O2-exposed HEK293T cell line model) approach. Some strains showed high yields of antioxidant activity by the DPPH assay (up to 10.7% IP/20.7 TE μg/mL) and by the β-carotene bleaching assay (up to 828.94 AAC), as well as significant content in phenolic (123.16 mg EAG/g DW) and flavonoid (900.60 mg EQR/g DW) compounds. Normalization of data in a "per cell" or "per cell volume" base might facilitate the comparison between strains. Additionally, most of the cyanobacterial extracts conferred some degree of protection to HEK293T cells against the H2O2-induced cytotoxicity. Freshwater Aphanizomenon gracile (LMECYA 009) and Aphanizomenon flos-aquae (LMECYA 088), terrestrial Nostoc (LMECYA 291) and wastewater Planktothrix mougeotii (LEGE 06224) seem to be promising strains for further investigation on cyanobacteria antioxidant potential.


Toxins (Basel)




Guerreiro A,Andrade MA,Menezes C,Vilarinho F,Dias E




Has Abstract


2020-08-26 00:00:00












  • Peptide Toxins in Solitary Wasp Venoms.

    abstract::Solitary wasps paralyze insects or spiders with stinging venom and feed the paralyzed preys to their larva. Accordingly, the venoms should contain a variety of constituents acting on nervous systems. However, only a few solitary wasp venoms have been chemically studied despite thousands of species inhabiting the plane...


    pub_type: 杂志文章,评审


    authors: Konno K,Kazuma K,Nihei K

    更新日期:2016-04-18 00:00:00

  • Water Thermodynamics of Peptide Toxin Binding Sites on Ion Channels.

    abstract::Peptide toxins isolated from venomous creatures, long prized as research tools due to their innate potency for ion channels, are emerging as drugs as well. However, it remains challenging to understand why peptide toxins bind with high potency to ion channels, to identify residues that are key for activity, and to imp...


    pub_type: 杂志文章


    authors: Shah B,Sindhikara D,Borrelli K,Leffler AE

    更新日期:2020-10-12 00:00:00

  • Protease Activity Profiling of Snake Venoms Using High-Throughput Peptide Screening.

    abstract::Snake venom metalloproteinases (SVMPs) and snake venom serine proteinases (SVSPs) are among the most abundant enzymes in many snake venoms, particularly among viperids. These proteinases are responsible for some of the clinical manifestations classically seen in viperid envenomings, including hemorrhage, necrosis, and...


    pub_type: 杂志文章


    authors: Kalogeropoulos K,Treschow AF,Auf dem Keller U,Escalante T,Rucavado A,Gutiérrez JM,Laustsen AH,Workman CT

    更新日期:2019-03-19 00:00:00

  • Expression of K1 Toxin Derivatives in Saccharomyces cerevisiae Mimics Treatment with Exogenous Toxin and Provides a Useful Tool for Elucidating K1 Mechanisms of Action and Immunity.

    abstract::Killer toxin K1 is a heterodimeric protein toxin secreted by Saccharomyces cerevisiae strains infected with the M1 double-stranded RNA 'killer' virus. After binding to a primary receptor at the level of the cell wall, K1 interacts with its secondary plasma membrane receptor Kre1p, eventually leading to an ionophoric d...


    pub_type: 杂志文章


    authors: Gier S,Schmitt MJ,Breinig F

    更新日期:2017-10-27 00:00:00

  • Eutrophication and Warming Boost Cyanobacterial Biomass and Microcystins.

    abstract::Eutrophication and warming are key drivers of cyanobacterial blooms, but their combined effects on microcystin (MC) concentrations are less studied. We tested the hypothesis that warming promotes cyanobacterial abundance in a natural plankton community and that eutrophication enhances cyanobacterial biomass and MC con...


    pub_type: 杂志文章


    authors: Lürling M,van Oosterhout F,Faassen E

    更新日期:2017-02-11 00:00:00

  • Tryptophan-Derived Uremic Toxins and Thrombosis in Chronic Kidney Disease.

    abstract::Patients with chronic kidney disease (CKD) display an elevated risk of thrombosis. Thrombosis occurs in cardiovascular events, such as venous thromboembolism, stroke, and acute coronary syndrome, and is a cause of hemodialysis vascular access dysfunction. CKD leads to the accumulation of uremic toxins, which exerts to...


    pub_type: 杂志文章,评审


    authors: Addi T,Dou L,Burtey S

    更新日期:2018-10-12 00:00:00

  • A monoclonal antibody based capture ELISA for botulinum neurotoxin serotype B: toxin detection in food.

    abstract::Botulism is a serious foodborne neuroparalytic disease, caused by botulinum neurotoxin (BoNT), produced by the anaerobic bacterium Clostridium botulinum. Seven toxin serotypes (A-H) have been described. The majority of human cases of botulism are caused by serotypes A and B followed by E and F. We report here a group ...


    pub_type: 杂志文章


    authors: Stanker LH,Scotcher MC,Cheng L,Ching K,McGarvey J,Hodge D,Hnasko R

    更新日期:2013-11-18 00:00:00

  • Editorial on the Special Issue "Comorbidities in Chronic Kidney Disease".

    abstract::With a mean worldwide prevalence of 13 [...]. ...


    pub_type: 社论


    authors: Noels H,Jankowski J

    更新日期:2020-06-11 00:00:00

  • Botulinum Neurotoxin A injections influence stretching of the gastrocnemius muscle-tendon unit in an animal model.

    abstract::Botulinum Neurotoxin A (BoNT-A) injections have been used for the treatment of muscle contractures and spasticity. This study assessed the influence of (BoNT-A) injections on passive biomechanical properties of the muscle-tendon unit. Mousegastrocnemius muscle (GC) was injected with BoNT-A (n = 18) or normal saline (n...


    pub_type: 杂志文章


    authors: Haubruck P,Mannava S,Plate JF,Callahan MF,Wiggins WF,Schmidmaier G,Tuohy CJ,Saul KR,Smith TL

    更新日期:2012-08-01 00:00:00

  • Rapid Radiations and the Race to Redundancy: An Investigation of the Evolution of Australian Elapid Snake Venoms.

    abstract::Australia is the stronghold of the front-fanged venomous snake family Elapidae. The Australasian elapid snake radiation, which includes approximately 100 terrestrial species in Australia, as well as Melanesian species and all the world's sea snakes, is less than 12 million years old. The incredible phenotypic and ecol...


    pub_type: 杂志文章


    authors: Jackson TN,Koludarov I,Ali SA,Dobson J,Zdenek CN,Dashevsky D,Op den Brouw B,Masci PP,Nouwens A,Josh P,Goldenberg J,Cipriani V,Hay C,Hendrikx I,Dunstan N,Allen L,Fry BG

    更新日期:2016-10-26 00:00:00

  • Deoxynivalenol Affects Cell Metabolism and Increases Protein Biosynthesis in Intestinal Porcine Epithelial Cells (IPEC-J2): DON Increases Protein Biosynthesis.

    abstract::Deoxynivalenol (DON) is a toxin found in cereals as well as in processed products such as pasta, and causes substantial economic losses for stock breeding as it induces vomiting, reduced feeding, and reduced growth rates in piglets. Oxidative phosphorylation, TCA-cycle, transcription, and translation have been hypothe...


    pub_type: 杂志文章


    authors: Nossol C,Landgraf P,Kahlert S,Oster M,Isermann B,Dieterich DC,Wimmers K,Dänicke S,Rothkötter HJ

    更新日期:2018-11-09 00:00:00

  • Chloroquine Analog Interaction with C2- and Iota-Toxin in Vitro and in Living Cells.

    abstract::C2-toxin from Clostridium botulinum and Iota-toxin from Clostridium perfringens belong both to the binary A-B-type of toxins consisting of two separately secreted components, an enzymatic subunit A and a binding component B that facilitates the entry of the corresponding enzymatic subunit into the target cells. The en...


    pub_type: 杂志文章


    authors: Kronhardt A,Beitzinger C,Barth H,Benz R

    更新日期:2016-08-10 00:00:00

  • Occurrence of pre- and post-harvest mycotoxins and other secondary metabolites in Danish maize silage.

    abstract::Maize silage is a widely used feed product for cattle worldwide, which may be contaminated with mycotoxins, pre- and post-harvest. This concerns both farmers and consumers. To assess the exposure of Danish cattle to mycotoxins from maize silage, 99 samples of whole-crop maize (ensiled and un-ensiled) were analyzed for...


    pub_type: 杂志文章


    authors: Storm IM,Rasmussen RR,Rasmussen PH

    更新日期:2014-07-31 00:00:00

  • Inhibition of Pore-Forming Proteins.

    abstract::Perforation of cellular membranes by pore-forming proteins can affect cell physiology, tissue integrity, or immune response. Since many pore-forming proteins are toxins or highly potent virulence factors, they represent an attractive target for the development of molecules that neutralize their actions with high effic...


    pub_type: 杂志文章,评审


    authors: Omersa N,Podobnik M,Anderluh G

    更新日期:2019-09-19 00:00:00

  • Intraspecific variation of centruroides edwardsii venom from two regions of Colombia.

    abstract::We report the first description studies, partial characterization, and intraspecific difference of Centruroides edwardsii, Gervais 1843, venom. C. edwardsii from two Colombian regions (Antioquia and Tolima) were evaluated. Both venoms showed hemolytic activity, possibly dependent of enzymatic active phospholipases, an...


    pub_type: 杂志文章


    authors: Estrada-Gómez S,Cupitra NI,Arango WM,Muñoz LJ

    更新日期:2014-07-14 00:00:00

  • Personalized Bilateral Upper Limb Essential Tremor Therapy with Botulinum Toxin Using Kinematics.

    abstract::Variability of multi-joint essential tremor (ET) between patients and within the two upper limbs makes a visual assessment for the determination of botulinum toxin type A (BoNT-A) injections challenging. Kinematic tremor analysis guidance has succeeded in overcoming this challenge by making effective long-term unilate...


    pub_type: 临床试验,杂志文章


    authors: Samotus O,Lee J,Jog M

    更新日期:2019-02-19 00:00:00

  • Uremic toxins enhance statin-induced cytotoxicity in differentiated human rhabdomyosarcoma cells.

    abstract::The risk of myopathy and rhabdomyolysis is considerably increased in statin users with end-stage renal failure (ESRF). Uremic toxins, which accumulate in patients with ESRF, exert cytotoxic effects that are mediated by various mechanisms. Therefore, accumulation of uremic toxins might increase statin-induced cytotoxic...


    pub_type: 杂志文章


    authors: Uchiyama H,Tsujimoto M,Shinmoto T,Ogino H,Oda T,Yoshida T,Furukubo T,Izumi S,Yamakawa T,Tachiki H,Minegaki T,Nishiguchi K

    更新日期:2014-09-03 00:00:00

  • Effect of Suspended Particulate Matter on the Accumulation of Dissolved Diarrhetic Shellfish Toxins by Mussels (Mytilus galloprovincialis) under Laboratory Conditions.

    abstract::In recent years, detection of trace amounts of dissolved lipophilic phycotoxins in coastal waters has been possible using solid phase adsorption toxin tracking (SPATT) samplers. To explore the contribution of dissolved diarrhetic shellfish toxins (DST) to the accumulation of toxins by cultivated bivalves, mussels (Myt...


    pub_type: 杂志文章


    authors: Li A,Li M,Qiu J,Song J,Ji Y,Hu Y,Wang S,Che Y

    更新日期:2018-07-03 00:00:00

  • Occurrence of Fusarium langsethiae and T-2 and HT-2 Toxins in Italian Malting Barley.

    abstract::T-2 and HT-2 toxins are two of the most toxic members of type-A trichothecenes, produced by a number of Fusarium species. The occurrence of these mycotoxins was studied in barley samples during a survey carried out in the 2011-2014 growing seasons in climatically different regions in Italy. The percentage of samples f...


    pub_type: 杂志文章


    authors: Morcia C,Tumino G,Ghizzoni R,Badeck FW,Lattanzio VM,Pascale M,Terzi V

    更新日期:2016-08-20 00:00:00

  • Murine Anorectic Response to Deoxynivalenol (Vomitoxin) Is Sex-Dependent.

    abstract::Deoxynivalenol (DON, vomitoxin), a common trichothecene mycotoxin found in cereal foods, dysregulates immune function and maintenance of energy balance. The purpose of this study was to determine if sex differences are similarly evident in DON's anorectic responses in mice. A bioassay for feed refusal, previously deve...


    pub_type: 杂志文章


    authors: Clark ES,Flannery BM,Pestka JJ

    更新日期:2015-07-29 00:00:00

  • Ochratoxin A management in vineyards by Lobesia botrana biocontrol.

    abstract::Grape berries attacked by Lobesia botrana larvae are more easily infected by Aspergillus section Nigri (black aspergilli) ochratoxigenic species. Two-year field trials were carried out in Apulia (Italy) to evaluate a bioinsecticide control strategy against L. botrana and the indirect effect on reducing ochratoxin A (...


    pub_type: 杂志文章


    authors: Cozzi G,Somma S,Haidukowski M,Logrieco AF

    更新日期:2013-01-02 00:00:00

  • Tissue Distribution and Elimination of Ciguatoxins in Tridacna maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus polynesiensis.

    abstract::Ciguatera is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs). Ciguatera-like poisoning events involving giant clams (Tridacna maxima) are reported occasionally from Pacific islands communities. The present study aimed at providing insights into CTXs tissue distribution and...


    pub_type: 杂志文章


    authors: Roué M,Darius HT,Ung A,Viallon J,Sibat M,Hess P,Amzil Z,Chinain M

    更新日期:2018-05-10 00:00:00

  • Toxicity of Cyanopeptides from Two Microcystis Strains on Larval Development of Astyanax altiparanae.

    abstract::Absorption and accumulation of bioavailable cyanobacterial metabolites (including cyanotoxins) are likely in fish after senescence and the rupturing of cells during bloom episodes. We determined the toxicity of cyanopeptides identified from two strains of Microcystis (M. panniformis MIRS-04 and M. aeruginosa NPDC-01) ...


    pub_type: 杂志文章


    authors: Fernandes K,Gomes A,Calado L,Yasui G,Assis D,Henry T,Fonseca A,Pinto E

    更新日期:2019-04-13 00:00:00

  • Microcystins alter chemotactic behavior in Caenorhabditis elegans by selectively targeting the AWA sensory neuron.

    abstract::Harmful algal blooms expose humans and animals to microcystins (MCs) through contaminated drinking water. While hepatotoxicity following acute exposure to MCs is well documented, neurotoxicity after sub-lethal exposure is poorly understood. We developed a novel statistical approach using a generalized linear model and...


    pub_type: 杂志文章


    authors: Moore CE,Lein PJ,Puschner B

    更新日期:2014-06-10 00:00:00

  • Toxin B Variants from Clostridium difficile Strains VPI 10463 and NAP1/027 Share Similar Substrate Profile and Cellular Intoxication Kinetics but Use Different Host Cell Entry Factors.

    abstract::Clostridium difficile induces antibiotic-associated diarrhea due to the release of toxin A (TcdA) and toxin B (TcdB), the latter being its main virulence factor. The epidemic strain NAP1/027 has an increased virulence attributed to different factors. We compared cellular intoxication by TcdBNAP1 with that by the refer...


    pub_type: 杂志文章


    authors: López-Ureña D,Orozco-Aguilar J,Chaves-Madrigal Y,Ramírez-Mata A,Villalobos-Jimenez A,Ost S,Quesada-Gómez C,Rodríguez C,Papatheodorou P,Chaves-Olarte E

    更新日期:2019-06-17 00:00:00

  • Autoproteolytic activation of bacterial toxins.

    abstract::Protease domains within toxins typically act as the primary effector domain within target cells. By contrast, the primary function of the cysteine protease domain (CPD) in Multifunctional Autoprocessing RTX-like (MARTX) and Clostridium sp. glucosylating toxin families is to proteolytically cleave the toxin and release...


    pub_type: 杂志文章,评审


    authors: Shen A

    更新日期:2010-05-01 00:00:00

  • Ochratoxin A and β2-microglobulin in BEN patients and controls.

    abstract::Ochratoxin A (OTA) is a mycotoxin naturally occurring in different foods. OTA is arguably a risk factor for Balkan endemic nephropathy (BEN). The aims of this study are to (1) test the OTA-BEN association in BEN-groups and controls and (2) determine whether urine β2-microglobulin, a marker of impaired ability of the k...


    pub_type: 杂志文章


    authors: Yordanova P,Wilfried K,Tsolova S,Dimitrov P

    更新日期:2010-04-01 00:00:00

  • The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice.

    abstract::Zearalenone (ZEN) is an estrogen-like mycotoxin produced by Fusarium that seriously compromises the safety of animal and human health. In this study, our aim was to evaluate the protective effect of Bacillus velezensis A2 against biochemical and pathological changes induced by zearalenone in mice. Kunming mice (n = 40...


    pub_type: 杂志文章


    authors: Wang N,Li P,Wang M,Chen S,Huang S,Long M,Yang S,He J

    更新日期:2018-10-31 00:00:00

  • Discovery of the Gene Encoding a Novel Small Serum Protein (SSP) of Protobothrops flavoviridis and the Evolution of SSPs.

    abstract::Small serum proteins (SSPs) are low-molecular-weight proteins in snake serum with affinities for various venom proteins. Five SSPs, PfSSP-1 through PfSSP-5, have been reported in Protobothrops flavoviridis ("habu", Pf) serum so far. Recently, we reported that the five genes encoding these PfSSPs are arranged in tandem...


    pub_type: 杂志文章


    authors: Inamaru K,Takeuchi A,Maeda M,Shibata H,Fukumaki Y,Oda-Ueda N,Hattori S,Ohno M,Chijiwa T

    更新日期:2020-03-12 00:00:00

  • Do Antibiotics Potentiate Proteases in Hemotoxic Snake Venoms?

    abstract::Antibiotics are often administered with antivenom following snakebite envenomings in order to avoid secondary bacterial infections. However, to this date, no studies have evaluated whether antibiotics may have undesirable potentiating effects on snake venom. Herein, we demonstrate that four commonly used antibiotics a...


    pub_type: 杂志文章


    authors: Sørensen CV,Knudsen C,Auf dem Keller UAD,Kalogeropoulos K,Gutiérrez-Jiménez C,Pucca MB,Arantes EC,Bordon KCF,Laustsen AH

    更新日期:2020-04-09 00:00:00