Abstract:
BACKGROUND:Babesia motasi is known as an etiological agent of human and ovine babesiosis. Diagnosis of babesiosis is traditionally performed by microscopy, examining Giemsa-stained thin peripheral blood smears. Rapid detection and accurate identification of species are desirable for clinical care and epidemiological studies. METHODS:An easy to operate molecular method, which requires less capital equipment and incorporates cross-priming amplification combined with a vertical flow (CPA-VF) visualization strip for rapid detection and identification of B. motasi. RESULTS:The CPA-VF targets the 18S rRNA gene and has a detection limit of 50 fg per reaction; no cross reaction was observed with other piroplasms infective to sheep or Babesia infective to humans. CPA-VF and real-time (RT)-PCR had sensitivities of 95.2% (95% confidence interval, CI 78.1-99.4%) and 90.5% (95% CI 72-97.6%) and specificities of 95.8 (95% CI 80.5-99.5%) and 97.9 (95% CI 83.5-99.9%), respectively, versus microscopy and nested (n) PCR combined with gene sequencing. The clinical performance of the CPA-VF assay was evaluated with field blood samples from sheep (n = 340) in Jintai county, Gansu Province, and clinical specimens (n = 492) obtained from patients bitten by ticks. CONCLUSIONS:Our results indicate that the CPA-VF is a rapid, accurate, nearly instrument-free molecular diagnostic approach for identification of B. motasi. Therefore, it could be an alternative technique for epidemiological investigations and diagnoses of ovine and/or human babesiosis caused by B. motasi, especially in resource-limited regions.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Wang J,Gao S,Zhang S,He X,Liu J,Liu A,Li Y,Liu G,Luo J,Guan G,Yin Hdoi
10.1186/s13071-020-04246-4subject
Has Abstractpub_date
2020-07-29 00:00:00pages
377issue
1issn
1756-3305pii
10.1186/s13071-020-04246-4journal_volume
13pub_type
杂志文章abstract:BACKGROUND:Chagas disease control programmes have decreased the prevalence of Chagas disease in Latin America. Together with migration to urban areas and increase in life expectancy, a new scenario for Chagas disease has emerged in Brazil with most patients currently elderly individuals living in urban areas. However, ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2929-y
更新日期:2018-06-19 00:00:00
abstract:BACKGROUND:Annual community-directed treatment with ivermectin (CDTI) have been carried out since 1999 in the Lekie division (central region of Cameroon where most cases of Loa-related post ivermectin severe adverse events were reported) as part of the joined activities of the African Programme for Onchocerciasis Contr...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-6-283
更新日期:2013-09-27 00:00:00
abstract:BACKGROUND:Sheep scab is caused by the ectoparasitic mite Psoroptes ovis which initiates a profound cutaneous inflammatory response, leading to the development of the skin lesions which are characteristic of the disease. Existing control strategies rely upon injectable endectocides and acaricidal dips but concerns over...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-30
更新日期:2012-02-08 00:00:00
abstract:BACKGROUND:Taenia multiceps is a harmful tapeworm and its larval form (coenurus cerebralis) is the causative agent of coenurosis, a disease affecting the health of herbivores, resulting in great economic loss to animal husbandry. Heat-shock proteins (HSPs), expressed in all prokaryotes and eukaryotes, act as molecular ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3352-8
更新日期:2019-03-12 00:00:00
abstract:BACKGROUND:Taenia ovis is a tapeworm that is mainly transmitted between dogs and sheep. Although T. ovis infection is not a public health issue, it causes a great financial loss due to condemnation of carcasses. The first outbreak of T. ovis infection in China occurred in 2015. Reassessment of adverse effects of T. ov...
journal_title:Parasites & vectors
pub_type: 信件
doi:10.1186/s13071-016-1700-5
更新日期:2016-07-26 00:00:00
abstract::Control and eventual elimination of human parasitic diseases in the People's Republic of China (P.R. China) requires novel approaches, particularly in the areas of diagnostics, mathematical modelling, monitoring, evaluation, surveillance and public health response. A comprehensive effort, involving the collaboration o...
journal_title:Parasites & vectors
pub_type: 杂志文章,评审
doi:10.1186/1756-3305-5-221
更新日期:2012-10-04 00:00:00
abstract:BACKGROUND:Taenia solium and Taenia saginata are zoonotic parasites of public health importance. Data on their occurrence in humans and animals in western Europe are incomplete and fragmented. In this study, we aimed to update the current knowledge on the epidemiology of these parasites in this region. METHODS:We cond...
journal_title:Parasites & vectors
pub_type: 杂志文章,评审
doi:10.1186/s13071-017-2280-8
更新日期:2017-07-21 00:00:00
abstract:BACKGROUND:Leishmania parasites cause severe human diseases known as leishmaniasis. These eukaryotic microorganisms possess an atypical chromosomal architecture and the regulation of gene expression occurs almost exclusively at post-transcriptional levels. Accordingly, sequencing of the genome of Leishmania major, and ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1329-4
更新日期:2016-02-08 00:00:00
abstract:BACKGROUND:Most studies on Enterocytozoon bieneusi are conducted based on the internal transcribed spacer (ITS) region of the rRNA gene, whereas some have examined E. bieneusi population structures. Currently, the population genetics of this pathogen in giant panda remains unknown. The objective of this study was to de...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2459-z
更新日期:2017-10-18 00:00:00
abstract:BACKGROUND:Infective larvae of the worldwide occurring zoonotic roundworm T. canis exhibit a marked affinity to the nervous tissues of paratenic hosts. In humans, most cases of neurotoxocarosis are considered to be caused by larvae of T. canis as T. cati larvae have rarely been found in the CNS in previous studies. How...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-194
更新日期:2014-04-22 00:00:00
abstract:BACKGROUND:Schistosoma japonicum is one of the major causative agents of schistosomiasis. The pairing of males and females leads to female sexual maturation and maintains this mature state. However, the mechanisms by which pairing facilitates sexual maturation are yet to be investigated. METHODS:Parasites isolated fro...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-177
更新日期:2014-04-10 00:00:00
abstract:BACKGROUND:Equine trypanosomiases are complex infectious diseases with overlapping clinical signs defined by their mode of transmission. Despite their economic impacts, these diseases have been neglected by the scientific community, the veterinary authorities and regulatory organizations. To fill the observed knowledge...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04192-1
更新日期:2020-06-23 00:00:00
abstract:BACKGROUND:The cattle tick Rhipicephalus (Boophilus) microplus is an economically important parasite of livestock. Effective control of ticks using acaricides is threatened by the emergence of resistance to many existing compounds. Several continuous R. microplus cell lines have been established and provide an under-ut...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1708-x
更新日期:2016-08-02 00:00:00
abstract:BACKGROUND:Arboviruses are a growing public health concern in Europe, with both endemic and exotic arboviruses expected to spread further into novel areas in the next decades. Predicting where future outbreaks will occur is a major challenge, particularly for regions where these arboviruses are not endemic. Spatial mod...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04339-0
更新日期:2020-09-10 00:00:00
abstract:BACKGROUND:A cytogenetic analysis of the new local triploid population of the caryophyllidean tapeworm Atractolytocestus huronensis, a unique parthenogenetic species with the ability to colonise new regions, was performed to understand the inner structure of its chromosome complement. METHODS:A karyotype analysis was ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3420-0
更新日期:2019-04-11 00:00:00
abstract:BACKGROUND:Thymidylate synthase is a housekeeping gene, designated ancient due to its role in DNA synthesis and ubiquitous phyletic distribution. The genomic sequences were characterized coding for thymidylate synthase in two species of the genus Trichinella, an encapsulating T. spiralis and a non-encapsulating T. pseu...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-175
更新日期:2014-04-09 00:00:00
abstract:BACKGROUND:Psoroptes cuniculi can parasitise the ear canal of the rabbit, and cause the afflicted animals to cease feeding and become severely debilitated, sometimes resulting in death. In this study, we examined the oxidative status and inflammatory level of the peripheral blood of rabbits infested with Psoroptes cuni...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-124
更新日期:2014-03-25 00:00:00
abstract:BACKGROUND:The most critical phase in sand fly colonization is the high mortality in the larval instars. In this study, we sought out strategies for improving the colonization of Nyssomyia neivai, one of the vectors of cutaneous leishmaniasis agent in South America. METHODS:A colony of Ny. neivai was established in th...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-1035-7
更新日期:2015-08-16 00:00:00
abstract:BACKGROUND:Schistosomiasis remains prevalent in Africa, Asia and South America with an estimated burden of 1.9 million disability-adjusted life years in 2016. Targeting snails as a key to success for schistosomiasis control has been widely approved, but the long-term quantitative effects of interventions on snail contr...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04273-1
更新日期:2020-08-05 00:00:00
abstract:BACKGROUND:Blastocystis sp. is one of the most prevalent parasites found in human stool and has been recently considered an opportunistic emerging pathogen in immunocompromised individuals. However, cases of invasive intestinal infections and skin rashes have been attributed to infection by Blastocystis sp in immunocom...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-207
更新日期:2014-04-29 00:00:00
abstract:BACKGROUND:Knowledge of the potential vector role of Culicidae mosquitoes in Germany is very scanty, and until recently it was generally assumed that they are not involved in the transmission of anthroponotic or zoonotic pathogens in this country. However, anticipated changes in the course of global warming and globali...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-14
更新日期:2012-01-11 00:00:00
abstract:BACKGROUND:Bravecto (fluralaner; MSD Animal Health) is a novel systemic ectoparasiticide for dogs providing long-acting flea- and tick-control after a single oral dose. Scalibor Protectorband (deltamethrin; MSD Animal Health) is a collar often used to reduce sandfly feeding for leishmaniasis prevention. This study inve...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-105
更新日期:2014-03-19 00:00:00
abstract:BACKGROUND:Understanding the dispersal dynamics of invasive mosquito species is fundamental to improve vector surveillance and to target control efforts. Aedes albopictus has been deemed a poor flyer as its range of active dispersal is generally assumed to be limited to a few hundred metres, while laboratory studies su...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3837-5
更新日期:2019-12-12 00:00:00
abstract:BACKGROUND:The red fox (Vulpes vulpes) is host to a community of zoonotic and other helminth species. Tracking their community structure and dynamics over decades is one way to monitor the long term risk of parasitic infectious diseases relevant to public and veterinary health. METHODS:We identified 17 helminth specie...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-166
更新日期:2014-04-03 00:00:00
abstract:BACKGROUND:The poultry red mite Dermanyssus gallinae negatively impacts bird welfare and health, and interferes with egg production and quality, while emerging acaricide resistance limits control options. Fluralaner, a novel miticide for administration in drinking water, is approved for control of D. gallinae infestati...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2956-8
更新日期:2018-06-25 00:00:00
abstract:BACKGROUND:The diagnosis of cutaneous leishmaniasis (CL) might be difficult, in particular in endemic areas where different species of Leishmania can cause lesions of very similar appearance and where other skin diseases with similar clinical symptoms occur. Even today, the parasitological diagnosis of CL remains the g...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-420
更新日期:2014-09-04 00:00:00
abstract::Major efforts are currently underway to develop novel, complementary methods to combat mosquito-borne diseases. Mosquito genetic control strategies (GCSs) have become an increasingly important area of research on account of their species-specificity, track record in targeting agricultural insect pests, and their envir...
journal_title:Parasites & vectors
pub_type: 杂志文章,评审
doi:10.1186/s13071-018-3222-9
更新日期:2018-12-24 00:00:00
abstract:BACKGROUND:To date there has been no evidence of mosquito-borne virus transmission of public health concern in the UK, despite the occurrence of more than 30 species of mosquito, including putative vectors of arboviruses. The saltmarsh mosquito Ochlerotatus detritus [syn. Aedes (Ochlerotatus) detritus] is locally commo...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1739-3
更新日期:2016-08-15 00:00:00
abstract:BACKGROUND:Ecto-Nucleoside Triphosphate Diphosphohydrolases (Ecto-NTPDases) are enzymes that hydrolyze tri- and/or di-phosphate nucleotides. Evidences point to their participation in Trypanosoma cruzi virulence and infectivity. In this work, we evaluate TcNTPDase-1 gene expression in comparison with ecto-NTPDase activi...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0463-0
更新日期:2014-10-05 00:00:00
abstract:BACKGROUND:Anaplasma ovis is a gram-negative, tick-borne obligate intraerythrocytic pathogen, which causes ovine anaplasmosis in small ruminants worldwide. VirB10 of A. ovis is an integral component of the Type IV Secretion System (T4SS). The T4SS is used by bacteria to transfer DNA and/or proteins undeviatingly into t...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-3975-9
更新日期:2020-02-27 00:00:00