Abstract：
Background:Chinese herbs have been widely used for thousands of years. In order to kill or control insects and fungus during the cultivation of herb plants, pesticides have played a significant role. More than 30 kinds of pesticides have been documented in the latest version of Chinese Pharmacopoeia. It is urgent to develop new analytical methods for pesticide detection. Methods:A fluorescent detection system was established by using Cerium based fluorescent polymer and Sm-CeO2. As a new doped nanozyme, Sm-CeO2 exhibits OPH-like activity to hydrolyze OPP pesticide. Results:The morphology of the prepared CFP and Sm-CeO2 were characterized. The optimal conditions for CFP synthesis are CeCl3 (16 mmol L-1, 200 μL), ATP (4 mmol L-1, 200 μL) and Tris buffer (5 mmol L-1, 500 μL, pH 8.0). Sm-CeO2 shows the best mimic activity to hydrolyze OPP pesticide at pH = 8.0. The results exhibit good linear relationship between fluorescent quenching effect and MP in the range of 2-50 μmol L-1. Finally, this fluorescent technique was successfully applied in Poria cocos and Semen Coicis sample. Conclusions:It is the first report on OPP detection by using CFP and doped nanozyme. The successful application in real sample indicates this method is a rapid, reliable strategy to detect OPP in Chinese herbs.
journal_name
Chin Medjournal_title
Chinese medicineauthors
Wei J,Xue Y,Dong J,Wang S,Hu H,Gao H,Li P,Wang Ydoi
10.1186/s13020-020-00304-2subject
Has Abstractpub_date
2020-03-10 00:00:00pages
22issn
1749-8546pii
304journal_volume
15pub_type
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