Abstract:
:Aerobic glycolysis plays a crucial role in cancer progression. Ketamine is often used for cancer pain relief in clinical settings. Moreover, ketamine inhibits proliferation and induces apoptosis in many cancer cell types. However, the anti-tumour mechanism of ketamine is still poorly understood. In the present study, we survey whether and how ketamine inhibits aerobic glycolysis in colon cancer cells. Glycolysis of colon cancer cells was determined by detecting the extracellular acidification rate in HT29 and SW480 cells. Quantitative real-time PCR was employed to determine mRNA expression. Calcium levels were detected with a Fluo-3 AM fluorescence kit. Micro-positron emission tomography/computed tomography (microPET/CT) imaging was employed to assess glycolysis in the tumours of the xenograft model. Ketamine treatment inhibited colon cancer cell viability and migration in HT29 and SW480 cells. Moreover, ketamine decreased aerobic glycolysis and decreased the expression of glycolysis-related proteins in HT29 and SW480 cells. MicroPET/CT demonstrated that ketamine decreased 18F-FDG uptake in the xenograft model. In addition, ketamine inhibited c-Myc expression and CaMK II phosphorylation and decreased calcium levels. Further, dizocilpine (an NMDAR inhibitor), and KN93 (a CaMK II inhibitor), decreased CaMK II phosphorylation, c-Myc expression, and cancer cell glycolysis; these results were similar to those with ketamine treatment. Furthermore, the anti-tumour effect of ketamine was counteracted by rapastinel (an NMDAR activator). Ketamine inhibited aerobic glycolysis in colon cancer cells probably by blocking the NMDA receptor-CaMK II-c-Myc pathway, thus attenuating colon cancer cell viability and migration.
journal_name
Clin Exp Pharmacol Physioljournal_title
Clinical and experimental pharmacology & physiologyauthors
Hu J,Duan W,Liu Ydoi
10.1111/1440-1681.13248subject
Has Abstractpub_date
2020-05-01 00:00:00pages
848-856issue
5eissn
0305-1870issn
1440-1681journal_volume
47pub_type
杂志文章abstract::1. We have tested the effects of 2 month oral treatment with the KATP opener, nitric oxide (NO) donor and anti-oxidant molecule nicorandil (0.1 mg/kg per day) on major physiological parameters and heart function of 4-, 12- and 24-month-old rats. 2. Several methods were used: (i) measurement of blood pressure using a n...
journal_title:Clinical and experimental pharmacology & physiology
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doi:10.1111/j.1440-1681.1977.tb02672.x
更新日期:1977-07-01 00:00:00
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journal_title:Clinical and experimental pharmacology & physiology
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journal_title:Clinical and experimental pharmacology & physiology
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doi:10.1111/j.1440-1681.1986.tb00337.x
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journal_title:Clinical and experimental pharmacology & physiology
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doi:10.1111/j.1440-1681.1988.tb01042.x
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pub_type: 杂志文章
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更新日期:2003-11-01 00:00:00
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doi:10.1111/j.1440-1681.1992.tb00459.x
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journal_title:Clinical and experimental pharmacology & physiology
pub_type: 信件
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更新日期:2018-05-01 00:00:00
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journal_title:Clinical and experimental pharmacology & physiology
pub_type: 杂志文章
doi:10.1111/j.1440-1681.1977.tb02621.x
更新日期:1977-05-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1046/j.1440-1681.2000.03383.x
更新日期:2000-12-01 00:00:00