Abstract:
BACKGROUND:The ST6Gal-I glycosyltransferase, which adds α2-6-linked sialic acids to N-glycosylated proteins is upregulated in a wide range of malignancies including ovarian cancer. Prior studies have shown that ST6Gal-I-mediated sialylation of select surface receptors remodels intracellular signaling to impart cancer stem cell (CSC) characteristics. However, the mechanisms that contribute to ST6Gal-I expression in stem-like cancer cells are poorly understood. RESULTS:Herein, we identify the master stem cell transcription factor, Sox2, as a novel regulator of ST6Gal-I expression. Interestingly, SOX2 and ST6GAL1 are located within the same tumor-associated amplicon, 3q26, and these two genes exhibit coordinate gains in copy number across multiple cancers including ~ 25% of ovarian serious adenocarcinomas. In conjunction with genetic co-amplification, our studies suggest that Sox2 directly binds the ST6GAL1 promoter to drive transcription. ST6Gal-I expression is directed by at least four distinct promoters, and we identified the P3 promoter as the predominant promoter utilized by ovarian cancer cells. Chromatin Immunoprecipitation (ChIP) assays revealed that Sox2 binds regions proximal to the P3 promoter. To confirm that Sox2 regulates ST6Gal-I expression, Sox2 was either overexpressed or knocked-down in various ovarian cancer cell lines. Sox2 overexpression induced an increase in ST6Gal-I mRNA and protein, as well as surface α2-6 sialylation, whereas Sox2 knock-down suppressed levels of ST6Gal-I mRNA, protein and surface α2-6 sialylation. CONCLUSIONS:These data suggest a process whereby SOX2 and ST6GAL1 are coordinately amplified in cancer cells, with the Sox2 protein then binding the ST6GAL1 promoter to further augment ST6Gal-I expression. Our collective results provide new insight into mechanisms that upregulate ST6Gal-I expression in ovarian cancer cells, and also point to the possibility that some of the CSC characteristics commonly attributed to Sox2 may, in part, be mediated through the sialyltransferase activity of ST6Gal-I.
journal_name
J Ovarian Resjournal_title
Journal of ovarian researchauthors
Dorsett KA,Jones RB,Ankenbauer KE,Hjelmeland AB,Bellis SLdoi
10.1186/s13048-019-0574-5subject
Has Abstractpub_date
2019-10-14 00:00:00pages
93issue
1issn
1757-2215pii
10.1186/s13048-019-0574-5journal_volume
12pub_type
杂志文章abstract:BACKGROUND:Experience on sentinel node mapping in ovarian tumors is very limited. We evaluated the sentinel node concept in ovarian tumors using intra-operativeTc-99m-Phytate injection and lymphoscintigraphy imaging. METHODS:Thirty-five patients with a pelvic mass due to an ovarian pathology were included in the study...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-016-0265-4
更新日期:2016-09-07 00:00:00
abstract:BACKGROUND:Low adiponectin levels in polycystic ovarian syndrome (PCOS) have been largely attributed to obesity which is common among these patients. In addition, evidence also suggests that low adiponectin in PCOS may be related to insulin resistance (IR) in these women. However, studies on the role of adiponectin in ...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-7-18
更新日期:2014-02-07 00:00:00
abstract::Both estrogens and androgens are involved in the development and normal functioning of the ovaries. It is also known that ovarian function is regulated by diet. The goal of this study was to estimate the expression of sex hormone receptors in ovaries of mice that were on a 9-month caloric restriction (alternate-day fe...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-015-0185-8
更新日期:2015-08-13 00:00:00
abstract:RATIONALE AND OBJECTIVES:To evaluate the impact of metabolic parameters in the peritoneal cavity on the likelihood of achieving complete tumor debulking in patients with ovarian and peritoneal cancers. MATERIALS AND METHODS:Forty-nine patients with ovarian and peritoneal cancers were included, who underwent pre-operat...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-020-00662-3
更新日期:2020-05-20 00:00:00
abstract:OBJECTIVE:To explore the risk factors for the recurrence of endometrioma and the risk factors for the recurrence of endometriosis-related pain after long-term follow-up. METHODS:This study retrospectively analyzed 358 women with endometriomas who had a minimum of 5-years follow up after laparoscopic endometrioma excis...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-019-0552-y
更新日期:2019-08-30 00:00:00
abstract:OBJECTIVE:Recently, 14-3-3 zeta protein was identified as a potential serum biomarker of epithelial ovarian cancer (EOC). The goal of this study was to investigate the clinical potential of 14-3-3 zeta protein for monitoring EOC progression compared with CA-125 and HE4. DESIGN:Prospective follow-up study. SETTING:Uni...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-6-79
更新日期:2013-11-15 00:00:00
abstract:BACKGROUND:This study aimed to identify the clinical and pathological characteristics and the possible prognostic factors for Chinese patients with early-stage ovarian endometrioid carcinoma. METHODS:The present study reviewed the medical records of patients who received initial treatment and a postoperative pathologi...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-017-0361-0
更新日期:2017-09-18 00:00:00
abstract::High-Grade Serous Ovarian Carcinoma (HGSOC) is the predominant histotype of epithelial ovarian cancer (EOC), characterized by advanced stage at diagnosis, frequent TP53 mutation, rapid progression, and high responsiveness to platinum-based-chemotherapy. To date, standard first-line-chemotherapy in advanced EOC include...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-016-0238-7
更新日期:2016-05-21 00:00:00
abstract:BACKGROUND:Recent studies have shown that microRNAs may regulate the ABCB1 gene (ATP-binding cassette, sub-family B [MDR/TAP], member 1). Computational programs have predicted that the 3'-untranslated region (3'-UTR) of ABCB1 contains a potential miRNA-binding site for miR-186. Here, we investigated the role of miR-186...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-015-0207-6
更新日期:2015-12-02 00:00:00
abstract:BACKGROUND:Ovarian cancer is the leading cause of gynecological cancer-related mortality. The novel oncogene S100A10 has been reported to be involved in cancer cell proliferation, invasion and metastasis. The role of S100A10 in ovarian cancer has not been well studied and the effect of S100A10 on chemotherapy remains u...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-019-0592-3
更新日期:2019-11-18 00:00:00
abstract:BACKGROUND:The value of this report is the identification of late recurrence with an extremely unusual combination of malignant transformation. In particular, the retroconversion of immature to mature teratoma as well as a somatic-type malignant transformation were both observed postchemotherapeutically in our case. C...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-018-0476-y
更新日期:2019-01-10 00:00:00
abstract:BACKGROUND:We aimed to examine the expression of miR-1307 in chemosensitive and chemoresistant epithelial ovarian cancer tissues and cell lines and to analyze the clinicopathological significance of miR-1307 in ovarian cancer. METHODS:MicroRNA microarray was used to screen differentially expressed microRNAs between th...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-015-0143-5
更新日期:2015-04-09 00:00:00
abstract:BACKGROUND:This study was designed to investigate leptin levels in the fluid in ovarian endometriomas (OEs) and to compare the expression of leptin and its receptors (OBR) in ovarian tissue affected by endometrioma in infertile women to its expression in the normal ovarian tissue of fertile controls without endometrios...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-7-2
更新日期:2014-01-08 00:00:00
abstract:BACKGROUND:Hypertensive disorders of pregnancy are the most common causes of adverse maternal & perinatal outcomes. Such investigations in resource limited settings would help to have great design strategies in preventing maternal and perinatal morbidity and mortality. AIM:To determine management outcome and factor as...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-015-0135-5
更新日期:2015-03-15 00:00:00
abstract:BACKGROUND:The polycystic ovary syndrome (PCOS) is a complex and heterogeneous endocrine condition characterized by hyperandrogenism, hyperinsulinemia, insulin resistance and chronic anovulation. Regulation and interaction of a multitude of genes required for follicular development are found to be altered in PCOS. Micr...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-6-36
更新日期:2013-05-15 00:00:00
abstract:BACKGROUND:Studies on the association between obesity and ovarian cancer survival have had conflicting results. We reviewed and quantitatively summarized the existing evidence, exploring potentially important sources of variability, such as the timing of body mass index (BMI) assessment, BMI cut points, references used...
journal_title:Journal of ovarian research
pub_type: 杂志文章,meta分析,评审
doi:10.1186/1757-2215-7-41
更新日期:2014-04-22 00:00:00
abstract::The diagnosis of primary angiosarcoma of ovary is still a challenge as it has no specific clinical symptoms and is easily confused with other malignant neoplasms in morphology. Here, we described a case of primary ovarian angiosarcoma and reviewed the literature. A 47-year-old female showed a left ovary mass. Grossly,...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-021-00771-7
更新日期:2021-01-28 00:00:00
abstract:BACKGROUND:The mechanism of aggressive character of ovarian cancer and unsuccessful treatment of women with this deadly disease has been recently explained by the theory of cancer stem cells (CSCs). It has been reported that ovarian carcinogenesis and progression of disease is associated with epithelial-mesenchymal tra...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-017-0306-7
更新日期:2017-02-23 00:00:00
abstract:BACKGROUND:The objective of this study was to build the transcriptomic profile of granulosa cells originating from follicles 6 to 9 mm in diameter in dairy cattle using microarrays. METHODS:Granulosa cells originating from three different phases of antral follicle growth were compared: growing (G), plateau (P) and atr...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-7-50
更新日期:2014-05-07 00:00:00
abstract:BACKGROUND:Despite advances in surgical aggressiveness and conventional chemotherapy, ovarian cancer remains the most lethal cause of gynecologic cancer mortality; consequently there is a need for new therapeutic agents and innovative treatment paradigms for the treatment of ovarian cancer. Several studies have demonst...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-016-0282-3
更新日期:2016-10-27 00:00:00
abstract:BACKGROUND:Ovarian hyperstimulation syndrome remains a serious complication during in vitro fertilization cycles if high dose human chorionic gonadotropin (hCG) is used to trigger ovulation in high responder patients. Though much of this risk is mitigated with trigger using gonadotropin releasing-hormone (GnRH) agonist...
journal_title:Journal of ovarian research
pub_type: 杂志文章,随机对照试验
doi:10.1186/s13048-019-0483-7
更新日期:2019-01-26 00:00:00
abstract:BACKGROUND:Altered androgen hormone homeostasis and androgen receptor (AR) activity have been implicated in ovarian carcinogenesis but the relationship between AR expression in ovarian cancer and clinical outcome remains unclear. METHODS:In this study, the prognostic impact of AR expression was investigated using immu...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-3-14
更新日期:2010-06-17 00:00:00
abstract:BACKGROUND:Upregulation of Cyclin dependent kinase 1 (CDK1) protein is closely related with the prognosis of several malignant tumors. Chk1-CDC25C-CDK1 signaling and P53-P21WAF1-CDK1 signaling pathways are closely related with the cell cycle G2/M phase regulation. The present study aimed to analyze the relationship bet...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-017-0356-x
更新日期:2017-09-12 00:00:00
abstract:BACKGROUND:The aim of this study was to evaluate the concentrations of IgG antibodies against Hsp60 and Hsp65 in sera of patients with ovarian cancer at various stages of clinical progress and for different histopathological types of disease. METHODS:Serum samples from 149 patients with ovarian carcinoma and 80 health...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-7-30
更新日期:2014-03-11 00:00:00
abstract:BACKGROUND:Neovascularization is necessary for follicular growth. Vascularization is first observed in preantral follicles, and thereafter the vasculature markedly increases in follicles undergoing development. Neovascularization includes angiogenesis and vasculogenesis. Vasculogenesis is the formation of new blood ves...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-7-10
更新日期:2014-01-21 00:00:00
abstract:BACKGROUND:Ovarian thecoma-fibroma groups (OTFG) are uncommon sex cord-stromal neoplasms. The objective of the study was to demonstrate clinical and sonographic features of OTFG and compare with surgical histopathology. METHODS:A total of 61 patients with surgically proven OTFG were enrolled in this retrospective stud...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-016-0291-2
更新日期:2016-11-22 00:00:00
abstract:BACKGROUND:Wnt-inducible signaling pathway protein 2 (WISP2) is a wnt1-induced signaling pathway protein 2. Although studies indicate that WISP2 may promote the development of various tumors, its role in ovarian cancer remains unclear. The objective of the current study was to analyze the effects of WISP2 on the prolif...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-020-00687-8
更新日期:2020-07-25 00:00:00
abstract:BACKGROUND:Degeneration of ovarian function is an obvious feature of female aging. In addition, studies have shown that autophagy decreases with age, and DNA methylation is a hallmark epigenetic pattern during aging. However, it is not clear whether the expression and DNA methylation of autophagy genes are involved in ...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-020-0615-0
更新日期:2020-02-03 00:00:00
abstract:BACKGROUND:Pelvic irradiation is essential for improving survival in women with pelvic malignancies despite inducing permanent ovarian damage. Ovarian transposition can be performed in premenopausal women in an attempt to preserve ovarian function. As uncertainty occurs over the proportion of women who are likely to be...
journal_title:Journal of ovarian research
pub_type: 杂志文章,meta分析,评审
doi:10.1186/1757-2215-7-69
更新日期:2014-06-25 00:00:00
abstract::RNA methylation can reverse the methylation modification at the RNA level, which is an extremely important epigenetic modification. The function and mechanism of YTHDF2, as a reader of m6A modification, in epithelial ovarian cancer (EOC) have not been elucidated so far. This study aimed to investigate how YTHDF2 and m...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-020-00717-5
更新日期:2020-09-18 00:00:00