Abstract:
BACKGROUND:Peripheral and uterine natural killer cells (pNK and uNK cells) are key players in the establishment and maintenance of pregnancy and are disturbed in patients with recurrent miscarriage (RM). Different immunologic risk factors have been proposed between patients with primary RM (pRM, no previous live birth) and secondary RM (sRM, ≥ 1 previous live birth). However, so far, the study populations mainly consisted of small subgroups. Therefore, we aimed to analyse pNK and uNK cells in a large, well defined study population within a prospective study. METHODS:In total, n = 575 RM patients (n = 393 pRM, n = 182 sRM) were screened according to a standard protocol for established risk factors as well as pNK and uNK cells. Peripheral blood levels of CD45+CD3-CD56+CD16+ NK cells were determined by flow cytometry and uterine CD56+ NK cells by immunohistochemistry in mid-luteal non-pregnant RM patients. Exclusion of patients with ≥1 established risk factor revealed n = 248 idiopathic RM patients (iRM, n = 167 primary iRM (ipRM), n = 81 secondary iRM (isRM)). RESULTS:Patients with pRM and ipRM showed significant higher absolute numbers and percentages of pNK cells compared to sRM and isRM patients (pRM/ipRM vs sRM/isRM, mean ± SD /μl: 239.1 ± 118.7/244.9 ± 112.9 vs 205.1 ± 107.9/206.0 ± 105.6, p = 0.004/ p = 0.009; mean ± SD %: 12.4 ± 5.5/12.8 ± 5.4 vs 11.1 ± 4.6/11.1 ± 4.3, p = 0.001; p = 0.002). Only patients with isRM showed significantly higher uNK levels compared to patients with ipRM (mean ± SD /mm2 288.4 ± 239.3 vs 218.2 ± 184.5, p = 0.044). CONCLUSIONS:The demonstrated differences in pNK and uNK cells in RM patients depending on previous live birth might indicate differences in NK cell recruitment and potentially different underlying immune disorders between pRM and sRM. As there is an overlap in the distribution of the NK cell results, further studies with focus on NK cell function are needed in order to clearly identify RM patients with distinct immune abnormalities. The clinical relevance of our findings should be interpreted cautiously until specificity and sensitivity are further evaluated.
journal_name
Reprod Biol Endocrinoljournal_title
Reproductive biology and endocrinology : RB&Eauthors
Toth B,Vomstein K,Togawa R,Böttcher B,Hudalla H,Strowitzki T,Daniel V,Kuon RJdoi
10.1186/s12958-019-0514-7subject
Has Abstractpub_date
2019-08-31 00:00:00pages
72issue
1issn
1477-7827pii
10.1186/s12958-019-0514-7journal_volume
17pub_type
杂志文章abstract:BACKGROUND:Claudin-4 (CLDN4) is one of several proteins that act as molecular mediators of embryo implantation. Recently, we examined immunolabeling of leukemia inhibitory factor (LIF) in the endometrial tissue of 52 IVF patients, and found that LIF staining intensity was strongly correlated with successful pregnancy i...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-7-30
更新日期:2009-04-19 00:00:00
abstract:BACKGROUND:Mares have an annual reproductive rhythm, with a phase of inactivity in midwinter. The aim of this study was to determine the impact of food restriction on physiological and metabolic hallmarks of this rhythm. METHODS:Over three successive years, 3 groups of 10 mares were kept under natural photoperiod. A '...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,随机对照试验
doi:10.1186/1477-7827-9-130
更新日期:2011-09-29 00:00:00
abstract:BACKGROUND:The G protein-coupled estrogen receptor (GPER) is thought to be involved in non-genomic estrogen responses as well as processes such as cell proliferation and migration. In this study, we analyzed GPER expression patterns from endometriosis samples and normal endometrial tissue samples and compared these exp...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-10-30
更新日期:2012-04-20 00:00:00
abstract:BACKGROUND:Relaxin levels in seminal plasma have been associated with positive effects on sperm motility and quality, and thus having potential roles in male fertility. However, the origin of seminal relaxin, within the male reproductive tract, and the moment of its release in the vicinity of spermatozoa remain unclear...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-015-0043-y
更新日期:2015-05-20 00:00:00
abstract:BACKGROUND:Mammalian oocyte meiotic maturation involves a number of important processes, including spindle assembly and migration, cortical reorganization and polar body extrusion. Numerous proteins contribute to these processes, but it is unknown whether MKlp2 (mitotic kinesin-like protein 2; also called KIF20A), a mi...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-11-117
更新日期:2013-12-21 00:00:00
abstract:BACKGROUND:The placenta is an essential organ that provides nutrients and oxygen to the developing fetus and removes toxic waste products from the fetal circulation. Maintaining placental blood osmotic pressure and blood flow is crucial for viable offspring. The renin-angiotensin system (RAS) in the placenta is a key p...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-015-0088-y
更新日期:2015-08-12 00:00:00
abstract:BACKGROUND:Testicular microliths are calcifications found within the seminiferous tubules. In humans, testicular microlithiasis (TM) has an unknown etiology but may be significantly associated with testicular germ cell tumors. Factors inducing microlith development may also, therefore, act as susceptibility factors for...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-7-88
更新日期:2009-08-27 00:00:00
abstract::During rat estrous cycle, the endometrium proliferates in response to sex steroids and specific endometrial epithelial cells undergo apoptosis in absence of embryonic factors. The central executioner of apoptosis is a family of aspartic acid-specific cysteine proteases known as caspases. Smac/DIABLO is released from t...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-1-59
更新日期:2003-08-27 00:00:00
abstract::Decidualization is an intricate biological process where extensive morphological, functional, and genetic changes take place in endometrial stromal cells to support the development of an implanting blastocyst. Deficiencies in decidualization are associated with pregnancy complications and reproductive diseases. Decidu...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,评审
doi:10.1186/s12958-017-0303-0
更新日期:2017-10-13 00:00:00
abstract:BACKGROUND:hCG is a wonder. Firstly, because hCG is such an extreme molecule. hCG is the most acidic glycoprotein containing the highest proportion of sugars. Secondly, hCG exists in 5 common forms. Finally, it has so many functions ranging from control of human pregnancy to human cancer. This review examines these mol...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,评审
doi:10.1186/1477-7827-10-24
更新日期:2012-03-28 00:00:00
abstract:BACKGROUND:To examine the effects of IVF, ICSI and FET, as well as in vitro culture, on the safety of offspring, this study was conducted from the perspective of genetic imprinting to investigate whether assisted reproductive technology would influence the parental and maternal imprinting genes. METHODS:Eighteen foetu...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-018-0344-z
更新日期:2018-07-05 00:00:00
abstract:BACKGROUND:Human uterine leiomyoma (fibroids; LYO) are the most common benign neoplasms in reproductive-aged women. Dysregulated extracellular matrix and irregular LYO reversion-inducing cysteine-rich protein with Kazal motifs (RECK) expression are thought to be mediated by aberrant microRNA (miR) expression. The relat...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-016-0180-y
更新日期:2016-08-17 00:00:00
abstract:BACKGROUND:Infertile men have higher levels of semen reactive oxygen species (ROS) than fertile men. High levels of semen ROS can cause sperm dysfunction, sperm DNA damage and reduced male reproductive potential. This study investigated the effects of supplementation with N-acetyl-cysteine (NAC) on the sperm quality, c...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12958-019-0468-9
更新日期:2019-02-16 00:00:00
abstract::Recent reports indicate that functional mouse oocytes and sperm can be derived in vitro from somatic cell lines. We hypothesize that in adult human ovaries, mesenchymal cells in the tunica albuginea (TA) are bipotent progenitors with a commitment for both primitive granulosa and germ cells. We investigated ovaries of ...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-2-20
更新日期:2004-04-28 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-10-20
更新日期:2012-03-21 00:00:00
abstract:BACKGROUND:The full maturational capability of mammalian oocytes is accompanied by nuclear and cytoplasmic modifications, which are associated with proliferation and differentiation of surrounding cumulus cells. These events are regulated on molecular level by the expression of target genes involved in signal transduct...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-017-0261-6
更新日期:2017-06-02 00:00:00
abstract:BACKGROUND:A limited number of publications have assessed the prevalence of hypertension (HTN) in polycystic ovary syndrome (PCOS) patients with inconclusive results. Since in general populations the occurrence of hypertension is related to age per se, we investigated the prevalence (P) / relative risk (RR) of HTN in p...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,评审
doi:10.1186/s12958-020-00576-1
更新日期:2020-03-17 00:00:00
abstract:BACKGROUND:The aim of this study was to evaluate the effects of vitrification on morpho-functional parameters (blastomere/chromatin integrity and bioenergy/oxidative potential) of mouse preimplantation embryos. METHODS:In vivo produced mouse (4/16-cell, morulae and blastocyst-stage) embryos were randomly divided into ...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-11-27
更新日期:2013-04-03 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,评审
doi:10.1186/1477-7827-2-31
更新日期:2004-06-16 00:00:00
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journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,评审
doi:10.1186/s12958-020-00617-9
更新日期:2020-06-06 00:00:00
abstract::Transient receptor potential cation channel, subfamily V, member 6 (TRPV6) is an epithelial Ca2+ channel protein expressed in calcium absorbing organs. In the present study, we investigated the expression and regulation of uterine and placental TRPV6 during gestation in rodents. Uterine TRPV6 peaked at pregnancy day (...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-7-49
更新日期:2009-05-21 00:00:00
abstract:BACKGROUND:Seminal plasma serves as a natural reservoir of antioxidants. It helps to remove excessive formation of reactive oxygen species (ROS) and consequently, reduce oxidative stress. Proteomic profiling of seminal plasma proteins is important to understand the molecular mechanisms underlying oxidative stress and s...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-11-85
更新日期:2013-09-03 00:00:00
abstract:BACKGROUND:L-carnitine (LC), and its acetylated form, acetyl L-carnitine (ALC), have immense functional capabilities to regulate the oxidative and metabolic status of the female reproductive system. The vulnerability of this system to free radicals demand for advanced strategies to combat them. For this purpose, the 'q...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章,评审
doi:10.1186/s12958-018-0323-4
更新日期:2018-01-26 00:00:00
abstract:BACKGROUND:Doxorubicin is a potent chemotherapeutic drug used against a variety of cancers. It acts through interaction with polymerases and topoisomerase II and free radical production. Doxorubicin activity is not specific to cancer cells and can also damage healthy cells, especially those undergoing rapid proliferati...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-10-79
更新日期:2012-09-11 00:00:00
abstract:BACKGROUND:Progesterone plays an important role in the proliferation and differentiation of human endometrial cells (hECs). Large-dose treatment with progesterone has been used for treatment of endometrial proliferative disorders. However, the mechanisms behind remain unknown. METHODS:To investigate the role of cyclin...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-7-144
更新日期:2009-12-07 00:00:00
abstract:BACKGROUND:Pregnancy-associated plasma protein-A2 (PAPP-A2) is consistently upregulated in the placentae of pregnancies complicated by preeclampsia and fetal growth restriction. The causes and significance of this upregulation remain unknown, but it has been hypothesized that it is a compensatory response to improve pl...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-018-0376-4
更新日期:2018-06-12 00:00:00
abstract:BACKGROUND:Previous studies have demonstrated that progestin-primed ovarian stimulation (PPOS) protocol was a feasible and efficient method in in vitro fertilization (IVF) cycle. However, its application in women with advanced age has not been determined yet. The purpose of this study was to investigate its efficacy in...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-019-0518-3
更新日期:2019-11-09 00:00:00
abstract:BACKGROUND:Patients hoping to preserve their fertility receive conservative treatment with high-dose medroxyprogesterone acetate (MPA) for well-differentiated endometrioid adenocarcinoma (EC) or atypical endometrial hyperplasia (AEH) . Such treatment generally involves frequent intrauterine operations, including dilati...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-015-0136-7
更新日期:2016-01-15 00:00:00
abstract:BACKGROUND:Macrophage migration inhibitory factor (MIF) has special pro-inflammatory roles, affecting the functions of macrophages and lymphocytes and counter-regulating the effects of glucocorticoids on the immune response. The conspicuous expression of MIF during human implantation and early embryonic development als...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/1477-7827-8-95
更新日期:2010-08-04 00:00:00
abstract:BACKGROUND:The aim of this study was to explore the impact of endometrial thickness change after progesterone administration on pregnancy outcome in patients transferred with single frozen-thawed blastocyst. METHODS:This observational cohort study included a total of 3091 patients undergoing their first frozen-thawed ...
journal_title:Reproductive biology and endocrinology : RB&E
pub_type: 杂志文章
doi:10.1186/s12958-019-0545-0
更新日期:2019-11-25 00:00:00