Biomarker of Aflatoxin Ingestion: ¹H NMR-Based Plasma Metabolomics of Dairy Cows Fed Aflatoxin B₁ with or without Sequestering Agents.


:The study applied ¹H NMR-based plasma metabolomics to identify candidate biomarkers of aflatoxin B1 (AFB₁) ingestion in dairy cows fed no sequestering agents and evaluate the effect of supplementing clay and/or a Saccharomyces cerevisiae fermentation product (SCFP) on such biomarkers. Eight lactating cows were randomly assigned to 1 of 4 treatments in a balanced 4 × 4 Latin square design with 2 squares. Treatments were: control, toxin (T; 1725 µg AFB₁/head/day), T with clay (CL; 200 g/head/day), and CL with SCFP (CL + SCFP; 35 g of SCFP/head/day). Cows in T, CL, and CL + SCFP were dosed with AFB₁ from d 26 to 30. The sequestering agents were top-dressed from d 1 to 33. On d 30 of each period, 15 mL of blood was taken from the coccygeal vessels and plasma samples were prepared by centrifugation. Compared to the control, T decreased plasma concentrations of alanine, acetic acid, leucine, arginine and valine. In contrast, T increased plasma ethanol concentration 3.56-fold compared to control. Treatment with CL tended to reduce sarcosine concentration, whereas treatment with CL + SCFP increased concentrations of mannose and 12 amino acids. Based on size of the area under the curve (AUC) of receiver operating characteristic and fold change (FC) analyses, ethanol was the most significantly altered metabolite in T (AUC = 0.88; FC = 3.56); hence, it was chosen as the candidate biomarker of aflatoxin ingestion in dairy cows fed no sequestering agent.


Toxins (Basel)




Ogunade I,Jiang Y,Adeyemi J,Oliveira A,Vyas D,Adesogan A




Has Abstract


2018-12-18 00:00:00












  • Rapid and Differential Evolution of the Venom Composition of a Parasitoid Wasp Depending on the Host Strain.

    abstract::Parasitoid wasps rely primarily on venom to suppress the immune response and regulate the physiology of their host. Intraspecific variability of venom protein composition has been documented in some species, but its evolutionary potential is poorly understood. We performed an experimental evolution initiated with the ...


    pub_type: 杂志文章


    authors: Cavigliasso F,Mathé-Hubert H,Kremmer L,Rebuf C,Gatti JL,Malausa T,Colinet D,Poirié M

    更新日期:2019-10-29 00:00:00

  • Bioaccumulation and Distribution of Indospicine and Its Foregut Metabolites in Camels Fed Indigofera spicata.

    abstract::In vitro experiments have demonstrated that camel foregut-fluid has the capacity to metabolize indospicine, a natural toxin which causes hepatotoxicosis, but such metabolism is in competition with absorption and outflow of indospicine from the different segments of the digestive system. Six young camels were fed Indig...


    pub_type: 杂志文章


    authors: Netzel G,Tan ETT,Yin M,Giles C,Yong KWL,Al Jassim R,Fletcher MT

    更新日期:2019-03-19 00:00:00

  • Natural Occurrence in Venomous Arthropods of Antimicrobial Peptides Active against Protozoan Parasites.

    abstract::Arthropoda is a phylum of invertebrates that has undergone remarkable evolutionary radiation, with a wide range of venomous animals. Arthropod venom is a complex mixture of molecules and a source of new compounds, including antimicrobial peptides (AMPs). Most AMPs affect membrane integrity and produce lethal pores in ...


    pub_type: 杂志文章,评审


    authors: Sabiá Júnior EF,Menezes LFS,de Araújo IFS,Schwartz EF

    更新日期:2019-09-25 00:00:00

  • Maternal-Fetal Cancer Risk Assessment of Ochratoxin A during Pregnancy.

    abstract::Increasing evidence has demonstrated that in utero exposure to environmental chemicals may interfere with fetal development and increase the risk of disease and cancer development later in life. Ochratoxin A (OTA) has been proven to induce diverse toxic effects including teratogenicity, carcinogenicity, immunotoxicity...


    pub_type: 杂志文章


    authors: Woo CS,El-Nezami H

    更新日期:2016-03-23 00:00:00

  • Detection and Quantification of ADP-Ribosylated RhoA/B by Monoclonal Antibody.

    abstract::Clostridium botulinum exoenzyme C3 is the prototype of C3-like ADP-ribosyltransferases that modify the GTPases RhoA, B, and C. C3 catalyzes the transfer of an ADP-ribose moiety from the co-substrate nicotinamide adenine dinucleotide (NAD) to asparagine-41 of Rho-GTPases. Although C3 does not possess cell-binding/-tran...


    pub_type: 杂志文章


    authors: Rohrbeck A,Fühner V,Schröder A,Hagemann S,Vu XK,Berndt S,Hust M,Pich A,Just I

    更新日期:2016-04-01 00:00:00

  • Dispersive Solid-Phase Extraction using Magnetic Carbon Nanotube Composite for the Determination of Emergent Mycotoxins in Urine Samples.

    abstract::Dispersive magnetic solid-phase extraction (DMSPE) has received growing attention for sample treatment preconcentration prior to the separation of analytes due to its many advantages. In the present work, the potential of DMSPE for the determination of emergent mycotoxins (enniatins A, A1, B and B1, and beauvericin) i...


    pub_type: 杂志文章


    authors: Arroyo-Manzanares N,Peñalver-Soler R,Campillo N,Viñas P

    更新日期:2020-01-15 00:00:00

  • Assessment of Dried Blood Spots for Multi-Mycotoxin Biomarker Analysis in Pigs and Broiler Chickens.

    abstract::Dried blood spots (DBSs), a micro-sampling technique whereby a drop of blood is collected on filter paper has multiple advantages over conventional blood sampling regarding the sampling itself, as well as transportation and storage. This is the first paper describing the development and validation of a method for the ...


    pub_type: 杂志文章


    authors: Lauwers M,Croubels S,De Baere S,Sevastiyanova M,Romera Sierra EM,Letor B,Gougoulias C,Devreese M

    更新日期:2019-09-18 00:00:00

  • Toxins of Prokaryotic Toxin-Antitoxin Systems with Sequence-Specific Endoribonuclease Activity.

    abstract::Protein translation is the most common target of toxin-antitoxin system (TA) toxins. Sequence-specific endoribonucleases digest RNA in a sequence-specific manner, thereby blocking translation. While past studies mainly focused on the digestion of mRNA, recent analysis revealed that toxins can also digest tRNA, rRNA an...


    pub_type: 杂志文章,评审


    authors: Masuda H,Inouye M

    更新日期:2017-04-14 00:00:00

  • Botulinum neurotoxins and botulism: a novel therapeutic approach.

    abstract::Specific treatment is not available for human botulism. Current remedial mainstay is the passive administration of polyclonal antibody to botulinum neurotoxin (BoNT) derived from heterologous species (immunized animal or mouse hybridoma) together with supportive and symptomatic management. The antibody works extracell...


    pub_type: 杂志文章,评审


    authors: Thanongsaksrikul J,Chaicumpa W

    更新日期:2011-05-01 00:00:00

  • Shiga toxin is transported into the nucleoli of intestinal epithelial cells via a carrier-dependent process.

    abstract::Shiga toxin (Stx) produced by the invasive Shigella dysenteriae serotype 1 (S. dysenteriae1) causes gastrointestinal and kidney complications. It has been assumed that Stx is released intracellularly after enterocyte invasion by S. dysenteriae1. However, there is little information about Stx distribution inside S. dys...


    pub_type: 杂志文章


    authors: Baibakov B,Murtazina R,Elowsky C,Giardiello FM,Kovbasnjuk O

    更新日期:2010-06-01 00:00:00

  • Pseudomonas Exotoxin Immunotoxins and Anti-Tumor Immunity: From Observations at the Patient's Bedside to Evaluation in Preclinical Models.

    abstract::Immunotoxins are protein drugs composed of a targeting domain genetically fused to a protein toxin. One killing domain being explored is a truncated Pseudomonas exotoxin A (PE). PE based immunotoxins are designed to kill cells directly by inhibiting their ability to synthesize proteins. However, observations from clin...


    pub_type: 杂志文章,评审


    authors: Leshem Y,Pastan I

    更新日期:2019-01-05 00:00:00

  • Functional Study of Haemophilus ducreyi Cytolethal Distending Toxin Subunit B.

    abstract::The Cytolethal Distending Toxin (CDT) is produced by many Gram-negative pathogenic bacteria responsible for major foodborne diseases worldwide. CDT induces DNA damage and cell cycle arrest in host-cells, eventually leading to senescence or apoptosis. According to structural and sequence comparison, the catalytic subun...


    pub_type: 杂志文章


    authors: Pons BJ,Loiseau N,Hashim S,Tadrist S,Mirey G,Vignard J

    更新日期:2020-08-19 00:00:00

  • Do Antibiotics Potentiate Proteases in Hemotoxic Snake Venoms?

    abstract::Antibiotics are often administered with antivenom following snakebite envenomings in order to avoid secondary bacterial infections. However, to this date, no studies have evaluated whether antibiotics may have undesirable potentiating effects on snake venom. Herein, we demonstrate that four commonly used antibiotics a...


    pub_type: 杂志文章


    authors: Sørensen CV,Knudsen C,Auf dem Keller UAD,Kalogeropoulos K,Gutiérrez-Jiménez C,Pucca MB,Arantes EC,Bordon KCF,Laustsen AH

    更新日期:2020-04-09 00:00:00

  • The Cytotoxicity Effect of Resveratrol: Cell Cycle Arrest and Induced Apoptosis of Breast Cancer 4T1 Cells.

    abstract::Resveratrol, a natural polyterpenoid, can scavenge reactive oxygen species in vivo to carry out the functions of antioxidation and antiaging. Resveratrol's anti-cancer capability has attracted widespread attention, but its molecular mechanism has not been systematically explained. In this study, by comparing the activ...


    pub_type: 杂志文章


    authors: Wu H,Chen L,Zhu F,Han X,Sun L,Chen K

    更新日期:2019-12-13 00:00:00

  • Detection of ochratoxin A using molecular beacons and real-time PCR thermal cycler.

    abstract::We developed a simple and cheap assay for quantitatively detecting ochratoxin A (OTA) in wine. A DNA aptamer available in literature was used as recognition probe in its molecular beacon form, i.e., with a fluorescence-quenching pair at the stem ends. Our aptabeacon could adopt a conformation allowing OTA binding, cau...


    pub_type: 杂志文章


    authors: Sanzani SM,Reverberi M,Fanelli C,Ippolito A

    更新日期:2015-03-09 00:00:00

  • Nano-Aptasensing in Mycotoxin Analysis: Recent Updates and Progress.

    abstract::Recent years have witnessed an overwhelming integration of nanomaterials in the fabrication of biosensors. Nanomaterials have been incorporated with the objective to achieve better analytical figures of merit in terms of limit of detection, linear range, assays stability, low production cost, etc. Nanomaterials can ac...


    pub_type: 杂志文章,评审


    authors: Rhouati A,Bulbul G,Latif U,Hayat A,Li ZH,Marty JL

    更新日期:2017-10-28 00:00:00

  • Spatial and temporal patterns in the seasonal distribution of toxic cyanobacteria in Western Lake Erie from 2002-2014.

    abstract::Lake Erie, the world's tenth largest freshwater lake by area, has had recurring blooms of toxic cyanobacteria for the past two decades. These blooms pose potential health risks for recreation, and impact the treatment of drinking water. Understanding the timing and distribution of the blooms may aid in planning by loc...


    pub_type: 杂志文章


    authors: Wynne TT,Stumpf RP

    更新日期:2015-05-12 00:00:00

  • Effects of Dietary Zearalenone on Oxidative Stress, Cell Apoptosis, and Tight Junction in the Intestine of Juvenile Grass Carp (Ctenopharyngodon idella).

    abstract::Zearalenone (ZEA) is a prevalent mycotoxin with high toxicity in animals. In order to study its effect on juvenile grass carp (Ctenopharyngodon idella), six diets supplemented with different levels of ZEA (0, 535, 1041, 1548, 2002, and 2507 μg/kg diet) for 10 weeks were studied to assess its toxicity on intestinal str...


    pub_type: 杂志文章


    authors: Wang YL,Zhou XQ,Jiang WD,Wu P,Liu Y,Jiang J,Wang SW,Kuang SY,Tang L,Feng L

    更新日期:2019-06-12 00:00:00

  • Peptide Toxins in Solitary Wasp Venoms.

    abstract::Solitary wasps paralyze insects or spiders with stinging venom and feed the paralyzed preys to their larva. Accordingly, the venoms should contain a variety of constituents acting on nervous systems. However, only a few solitary wasp venoms have been chemically studied despite thousands of species inhabiting the plane...


    pub_type: 杂志文章,评审


    authors: Konno K,Kazuma K,Nihei K

    更新日期:2016-04-18 00:00:00

  • No Association between Mycotoxin Exposure and Autism: A Pilot Case-Control Study in School-Aged Children.

    abstract::Evaluation of environmental risk factors in the development of autism spectrum disorder (ASD) is needed for a more complete understanding of disease etiology and best approaches for prevention, diagnosis, and treatment. A pilot experiment in 54 children (n = 25 ASD, n = 29 controls; aged 12.4 ± 3.9 years) screened for...


    pub_type: 杂志文章


    authors: Duringer J,Fombonne E,Craig M

    更新日期:2016-07-20 00:00:00

  • Botulinum Neurotoxin A injections influence stretching of the gastrocnemius muscle-tendon unit in an animal model.

    abstract::Botulinum Neurotoxin A (BoNT-A) injections have been used for the treatment of muscle contractures and spasticity. This study assessed the influence of (BoNT-A) injections on passive biomechanical properties of the muscle-tendon unit. Mousegastrocnemius muscle (GC) was injected with BoNT-A (n = 18) or normal saline (n...


    pub_type: 杂志文章


    authors: Haubruck P,Mannava S,Plate JF,Callahan MF,Wiggins WF,Schmidmaier G,Tuohy CJ,Saul KR,Smith TL

    更新日期:2012-08-01 00:00:00

  • Cytolethal Distending Toxin Subunit B: A Review of Structure-Function Relationship.

    abstract::The Cytolethal Distending Toxin (CDT) is a bacterial virulence factor produced by several Gram-negative pathogenic bacteria. These bacteria, found in distinct niches, cause diverse infectious diseases and produce CDTs differing in sequence and structure. CDTs have been involved in the pathogenicity of the associated b...


    pub_type: 杂志文章,评审


    authors: Pons BJ,Vignard J,Mirey G

    更新日期:2019-10-12 00:00:00

  • Development of Anti-Idiotypic Nanobody-Phage Based Immuno-Loop-Mediated Isothermal Amplification Assay for Aflatoxins in Peanuts.

    abstract::Aflatoxin contamination in agricultural products has posed serious health hazards and brought huge economic loss in the food and feed industries. Monitoring aflatoxins in various foods and feeds has become a crucial means to protect public health. This study aimed to report an immuno-loop-mediated isothermal amplifica...


    pub_type: 杂志文章


    authors: Lei J,Han X,Tang X,Wang H,Zhang Q

    更新日期:2020-09-02 00:00:00

  • Bioactivity of Natural and Engineered Antimicrobial Peptides from Venom of the Scorpions Urodacus yaschenkoi and U. manicatus.

    abstract::The spread of multidrug-resistant human pathogens has drawn attention towards antimicrobial peptides (AMPs), which are major players in the innate immune systems of many organisms, including vertebrates, invertebrates, plants and microbes. Scorpion venom is an abundant source of novel and potent AMPs. Here, we investi...


    pub_type: 杂志文章


    authors: Luna-Ramirez K,Tonk M,Rahnamaeian M,Vilcinskas A

    更新日期:2017-01-06 00:00:00

  • Mass Spectrometry-Based Method of Detecting and Distinguishing Type 1 and Type 2 Shiga-Like Toxins in Human Serum.

    abstract::Shiga-like toxins (verotoxins) are responsible for the virulence associated with a variety of foodborne bacterial pathogens. Direct detection of toxins requires a specific and sensitive technique. In this study, we describe a mass spectrometry-based method of analyzing the tryptic decapeptides derived from the non-tox...


    pub_type: 杂志文章


    authors: Silva CJ,Erickson-Beltran ML,Skinner CB,Patfield SA,He X

    更新日期:2015-12-02 00:00:00

  • A Novel Uremic Score Reflecting Accumulation of Specific Uremic Toxins More Precisely Predicts One-Year Mortality after Hemodialysis Commencement: A Retrospective Cohort Study.

    abstract::Uremic toxins (UTs) generally accumulate in patients developing end-stage renal disease (ESRD). Although some kinds of UTs cause early death after starting hemodialysis (HD), it remains unknown whether the degree of excessive accumulation of various UTs is associated with worsening of prognosis. We retrospectively con...


    pub_type: 杂志文章


    authors: Arai Y,Shioji S,Tanaka H,Katagiri D,Hinoshita F

    更新日期:2020-10-01 00:00:00

  • A monoclonal antibody based capture ELISA for botulinum neurotoxin serotype B: toxin detection in food.

    abstract::Botulism is a serious foodborne neuroparalytic disease, caused by botulinum neurotoxin (BoNT), produced by the anaerobic bacterium Clostridium botulinum. Seven toxin serotypes (A-H) have been described. The majority of human cases of botulism are caused by serotypes A and B followed by E and F. We report here a group ...


    pub_type: 杂志文章


    authors: Stanker LH,Scotcher MC,Cheng L,Ching K,McGarvey J,Hodge D,Hnasko R

    更新日期:2013-11-18 00:00:00

  • Snake Venomics and Antivenomics of Bothrops diporus, a Medically Important Pitviper in Northeastern Argentina.

    abstract::Snake species within genus Bothrops are responsible for more than 80% of the snakebites occurring in South America. The species that cause most envenomings in Argentina, B. diporus, is widely distributed throughout the country, but principally found in the Northeast, the region with the highest rates of snakebites. Th...


    pub_type: 杂志文章


    authors: Gay C,Sanz L,Calvete JJ,Pla D

    更新日期:2015-12-25 00:00:00

  • Effect of Suspended Particulate Matter on the Accumulation of Dissolved Diarrhetic Shellfish Toxins by Mussels (Mytilus galloprovincialis) under Laboratory Conditions.

    abstract::In recent years, detection of trace amounts of dissolved lipophilic phycotoxins in coastal waters has been possible using solid phase adsorption toxin tracking (SPATT) samplers. To explore the contribution of dissolved diarrhetic shellfish toxins (DST) to the accumulation of toxins by cultivated bivalves, mussels (Myt...


    pub_type: 杂志文章


    authors: Li A,Li M,Qiu J,Song J,Ji Y,Hu Y,Wang S,Che Y

    更新日期:2018-07-03 00:00:00

  • Microcystins alter chemotactic behavior in Caenorhabditis elegans by selectively targeting the AWA sensory neuron.

    abstract::Harmful algal blooms expose humans and animals to microcystins (MCs) through contaminated drinking water. While hepatotoxicity following acute exposure to MCs is well documented, neurotoxicity after sub-lethal exposure is poorly understood. We developed a novel statistical approach using a generalized linear model and...


    pub_type: 杂志文章


    authors: Moore CE,Lein PJ,Puschner B

    更新日期:2014-06-10 00:00:00