Protease resistance of food proteins: a mixed picture for predicting allergenicity but a useful tool for assessing exposure.

Abstract:

Background:Susceptibility to pepsin digestion of candidate transgene products is regarded an important parameter in the weight-of-evidence approach for allergenicity risk assessment of genetically modified crops. It has been argued that protocols used for this assessment should better reflect physiological conditions encountered in representative food consumption scenarios. Aim:To evaluate whether inclusion of more physiological conditions, such as sub-optimal and lower pepsin concentrations, in combination with pancreatin digestion, improved the performance of digestibility protocols used in characterization of protein stability. Methods:Four pairs of established allergens and their related non/weakly-allergenic counterparts (seed albumins, muscle tropomyosins, plant lipid transfer proteins [LTP] and collagens) plus fish parvalbumin, were subjected to nine combinations of pH (1.2-2.5-4.0) and pepsin-to-protein ratio (PPR: 10-1-0.1 U/µg) for pepsin digestion, followed by pancreatin digestion in the presence of bile salts. Digestion was monitored by SDS-PAGE in conjunction with Coomassie staining and immunoblotting using rabbit antisera and human IgE. Results:At pH 4.0 and at PPR 0.1 most proteins, both allergen and non-allergen, were highly resistant to pepsin. Under conditions known to favor pepsin proteolysis, the established major allergens Ara h 2, Pru p 3 and Pen a 1 were highly resistant to proteolysis, while the allergen Cyp c 1 was not. However, this resistance to pepsin digestion only made Ara h 2 and to a lesser extent Pen a 1 and Pru p 3 stand out compared to their non-allergenic counterparts. Largely irrespective of preceding pepsin digestion conditions, pancreatin digestion was very effective for all tested proteins, allergens and non-allergens, except for Cyp c 1 and bovine collagen. Conclusions:Sub-optimal pH, low pepsin-to protein ratio, and sequential pepsin and pancreatin digestion protocols do not improve the predictive value in distinguish allergens from non-allergens. Digestion conditions facilitating such distinction differ per protein pair.

journal_name

Clin Transl Allergy

authors

Akkerdaas J,Totis M,Barnett B,Bell E,Davis T,Edrington T,Glenn K,Graser G,Herman R,Knulst A,Ladics G,McClain S,Poulsen LK,Ranjan R,Rascle JB,Serrano H,Speijer D,Wang R,Pereira Mouriès L,Capt A,van Ree R

doi

10.1186/s13601-018-0216-9

subject

Has Abstract

pub_date

2018-08-10 00:00:00

pages

30

issn

2045-7022

pii

216

journal_volume

8

pub_type

杂志文章
  • Pathogenesis and diagnosis of delayed-type drug hypersensitivity reactions, from bedside to bench and back.

    abstract::Drug hypersensitivity reactions (DHR) have been present since the advent of drugs. In particular T-cell mediated delayed-type hypersensitivity reactions represent a heterogeneous clinical entity with a diverse pathogenesis and result in a considerable burden of morbidity and mortality not only driven by the reactions ...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章,评审

    doi:10.1186/s13601-015-0073-8

    authors: Schrijvers R,Gilissen L,Chiriac AM,Demoly P

    更新日期:2015-09-03 00:00:00

  • Characterization of a subgroup of non-type 2 asthma with cow's milk hypersensitivity in young subjects.

    abstract:Background:Asthma with atopy is often characterized by type 2 inflammation but less progress has been made in defining non-type 2 asthma. We have previously identified a subgroup of young non-atopic asthmatics with perceived food hypersensitivity and poor asthma control. Objective:Our aim was to further characterize t...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/s13601-019-0250-2

    authors: Tsolakis N,Nordvall L,Janson C,Rydell N,Malinovschi A,Alving K

    更新日期:2019-02-22 00:00:00

  • The association between airborne pollen monitoring and sensitization in the hot desert climate.

    abstract:Background:Pollen is a major cause of allergic respiratory diseases. In Qatar, data on the presence and prevalence of allergenic airborne types of pollen is quite limited. Methods:The study aimed to determine and correlate the most frequently implicated airborne pollen detected by aerobiological monitoring samplers in...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/s13601-020-00339-6

    authors: Al-Nesf MA,Gharbi D,Mobayed HM,Dason BR,Mohammed Ali R,Taha S,Tuffaha A,Adeli M,Sattar HA,Trigo MDM

    更新日期:2020-08-10 00:00:00

  • Human in vitro induced T regulatory cells and memory T cells share common demethylation of specific FOXP3 promoter region.

    abstract:BACKGROUND:The FOXP3 gene is the master regulator for T regulatory cells and is under tight DNA methylation control at the Treg specific demethylated region (TSDR) in its first intron. This said, methylation of its promoter region, the significance of which is unknown, has also been associated with various immune-relat...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/s13601-015-0079-2

    authors: Bégin P,Schulze J,Baron U,Olek S,Bauer RN,Passerini L,Baccheta R,Nadeau KC

    更新日期:2015-10-20 00:00:00

  • Erratum to: Scaling up strategies of the chronic respiratory disease programme of the European Innovation Partnership on Active and Healthy Ageing (Action Plan B3: Area 5).

    abstract::[This corrects the article DOI: 10.1186/s13601-016-0116-9.]. ...

    journal_title:Clinical and translational allergy

    pub_type: 已发布勘误

    doi:10.1186/s13601-016-0135-6

    authors: Bousquet J,Farrell J,Crooks G,Hellings P,Bel EH,Bewick M,Chavannes NH,de Sousa JC,Cruz AA,Haahtela T,Joos G,Khaltaev N,Malva J,Muraro A,Nogues M,Palkonen S,Pedersen S,Robalo-Cordeiro C,Samolinski B,Strandberg T,Va

    更新日期:2017-02-20 00:00:00

  • Health Related Quality of Life among schoolchildren aged 12-13 years in relation to food hypersensitivity phenotypes: a population-based study.

    abstract:BACKGROUND:While Health Related Quality of Life has been investigated among children with IgE-mediated food allergy, less is known about quality of life among children with other types of hypersensitivity to food. The aim of this study was to investigate Health Related Quality of Life (HRQL) in children with and withou...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/s13601-017-0156-9

    authors: Strinnholm Å,Hedman L,Winberg A,Jansson SA,Lindh V,Rönmark E

    更新日期:2017-07-03 00:00:00

  • Sinus computed tomography predicts clinical response to corticosteroids in chronic rhinosinusitis with nasal polyps.

    abstract:Background:Chronic rhinosinusitis with nasal polyps (CRSwNP) is a heterogeneous inflammatory disease usually characterized by chronic eosinophilia in the sinonasal mucosa, which often requires glucocorticoid (GC) therapy. However, the therapeutic response varies markedly between individuals. The objective of this study...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/s13601-018-0211-1

    authors: Hong H,Wang D,Tan KS,Zheng R,Chen F,Gao W,He H,Shi J,Fan Y,Yang Q,Sun Y

    更新日期:2018-07-02 00:00:00

  • Protocol for a double-blind randomised controlled trial of low dose intradermal grass pollen immunotherapy versus a histamine control on symptoms and medication use in adults with seasonal allergic rhinitis (PollenLITE).

    abstract:BACKGROUND:Subcutaneous immunotherapy with high dose grass pollen (typically microgram quantities) was first described over 100 years ago. This treatment suppresses allergen-induced cutaneous late responses, with lesser effects on early responses. We previously reported that repeated 2-weekly intradermal injections of ...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/2045-7022-3-27

    authors: Slovick A,Douiri A,Kelly J,Guerra A,Muir R,Tsioulos K,Murphy C,Shamji MH,Ying S,Durham SR,Till SJ

    更新日期:2013-08-21 00:00:00

  • Dual exposure to smoking and household air pollution is associated with an increased risk of severe asthma in adults in Brazil.

    abstract:Background:The relationship between smoking, household pollution, dual exposure and severity of asthma in adults has not been sufficiently studied. We examined and compared the effects of cigarette smoking, domestic wood burning pollution and dual exposure (tobacco and wood burning) upon asthma severity in adults. Met...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/s13601-018-0235-6

    authors: Fernandes AGO,de Souza-Machado C,Pinheiro GP,de Oliva ST,Mota RCL,de Lima VB,Cruz CS,Chatkin JM,Cruz ÁA

    更新日期:2018-12-10 00:00:00

  • Preventing food allergy: protocol for a rapid systematic review.

    abstract:BACKGROUND:The European Academy of Allergy and Clinical Immunology is developing guidelines about how to prevent and manage food allergy. As part of the guidelines development process, a systematic review is planned to examine published research about the prevention of food allergy. This systematic review is one of sev...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/2045-7022-3-10

    authors: de Silva D,Panesar SS,Thusu S,Rader T,Halken S,Muraro A,Sheikh A

    更新日期:2013-03-28 00:00:00

  • The placebo effect in allergen-specific immunotherapy trials.

    abstract:BACKGROUND:Double-blind, placebo-controlled (DBPC) trials are the gold standard for demonstrating clinical efficacy and tolerability. The placebo effect, although an important feature in placebo-controlled studies, has never been systematically investigated in allergen-specific immunotherapy (SIT) studies. This study w...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/2045-7022-3-42

    authors: Narkus A,Lehnigk U,Haefner D,Klinger R,Pfaar O,Worm M

    更新日期:2013-12-21 00:00:00

  • Application of the adverse outcome pathway (AOP) concept to structure the available in vivo and in vitro mechanistic data for allergic sensitization to food proteins.

    abstract:BACKGROUND:The introduction of whole new foods in a population may lead to sensitization and food allergy. This constitutes a potential public health problem and a challenge to risk assessors and managers as the existing understanding of the pathophysiological processes and the currently available biological tools for ...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章,评审

    doi:10.1186/s13601-017-0152-0

    authors: van Bilsen JHM,Sienkiewicz-Szłapka E,Lozano-Ojalvo D,Willemsen LEM,Antunes CM,Molina E,Smit JJ,Wróblewska B,Wichers HJ,Knol EF,Ladics GS,Pieters RHH,Denery-Papini S,Vissers YM,Bavaro SL,Larré C,Verhoeckx KCM,Roggen EL

    更新日期:2017-05-12 00:00:00

  • Prevalence of asthma in Portugal - The Portuguese National Asthma Survey.

    abstract:BACKGROUND:Asthma is a frequent chronic respiratory disease in both children and adults. However, few data on asthma prevalence are available in Portugal. The Portuguese National Asthma Survey is the first nationwide study that uses standardized methods. We aimed to estimate the prevalence of current asthma in the Port...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/2045-7022-2-15

    authors: Sa-Sousa A,Morais-Almeida M,Azevedo LF,Carvalho R,Jacinto T,Todo-Bom A,Loureiro C,Bugalho-Almeida A,Bousquet J,Fonseca JA

    更新日期:2012-08-29 00:00:00

  • Stevens-Johnson syndrome/toxic epidermal necrolysis and erythema multiforme drug-related hospitalisations in a national administrative database.

    abstract:Background:Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN) and erythema multiforme (EM) are immunologically-mediated dermatological disorders commonly triggered by drug exposure and/or other external agents. We aimed to characterise SJS/TEN- and EM-drug-related hospitalisations in a nationwide administrat...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/s13601-017-0188-1

    authors: Sousa-Pinto B,Araújo L,Freitas A,Correia O,Delgado L

    更新日期:2018-01-22 00:00:00

  • Study of atopic multimorbidity in subjects with rhinitis using multiplex allergen component analysis.

    abstract:Background:Rhinitis is a common problem within the population. Many subjects with rhinitis also have atopic multimorbidity, such as asthma and eczema. The purpose of this investigation was to compare subjects with only rhinitis to those that have rhinitis, asthma and/or eczema in relation to immunoglobulin E (IgE) sens...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/s13601-020-0311-6

    authors: Blöndal V,Sundbom F,Borres MP,Högman M,Alving K,Malinovschi A,Janson C

    更新日期:2020-02-21 00:00:00

  • Relationship between vitamin D and chronic spontaneous urticaria: a systematic review.

    abstract:Background:Vitamin D has been reported to be associated with many allergic diseases. There are a limited number of the studies of vitamin D supplementation in patients with chronic spontaneous urticaria (CSU). This study aims to study the relationship between vitamin D and CSU in terms of serum vitamin D levels, and th...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/s13601-018-0234-7

    authors: Tuchinda P,Kulthanan K,Chularojanamontri L,Arunkajohnsak S,Sriussadaporn S

    更新日期:2018-12-04 00:00:00

  • Oral administration of Lactobacillus paracasei NCC 2461 for the modulation of grass pollen allergic rhinitis: a randomized, placebo-controlled study during the pollen season.

    abstract:BACKGROUND:The efficacy of Lactobacillus paracasei NCC 2461 in modulating allergic rhinitis was previously evaluated in two exploratory clinical studies. Oral administration with NCC 2461 reduced specific subjective symptoms following nasal provocation tests with controlled grass pollen allergen concentrations. Our aim...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/s13601-015-0085-4

    authors: Nembrini C,Singh A,De Castro CA,Mercenier A,Nutten S

    更新日期:2015-12-09 00:00:00

  • Lung function in severe pediatric asthma: a longitudinal study in children and adolescents in Brazil.

    abstract:Background:In severe asthma, high doses of inhaled corticosteroids (ICS) are used in order to achieve clinical and functional control. This study aimed to evaluate lung function in outpatients (children and adolescents) with severe asthma in Brazil, all of whom were treated with high doses of ICS. We evaluated all spir...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/s13601-017-0183-6

    authors: Versiani Nunes Pinheiro de Queiroz M,Gonçalves Alvim C,Cruz ÁA,de Lima Belizário Facury Lasmar LM

    更新日期:2017-12-15 00:00:00

  • Endotype-driven treatment in chronic upper airway diseases.

    abstract::Rhinitis and rhinosinusitis are the two major clinical entities of chronic upper airway disease. Chronic rhinosinusitis (CRS) and allergic rhinitis (AR) affect respectively up to 10 and 30% of the total population, hence being associated with an important socio-economic burden. Different phenotypes of rhinitis and CRS...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章,评审

    doi:10.1186/s13601-017-0157-8

    authors: De Greve G,Hellings PW,Fokkens WJ,Pugin B,Steelant B,Seys SF

    更新日期:2017-07-12 00:00:00

  • Association of elevated fractional exhaled nitric oxide concentration and blood eosinophil count with severe asthma exacerbations.

    abstract:Background:Blood eosinophil count (BEC) and fractional exhaled nitric oxide (FeNO) concentration are established biomarkers in asthma, associated particularly with the risk of exacerbations. We evaluated the relationship of BEC and FeNO as complementary and independent biomarkers of severe asthma exacerbations. Method...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/s13601-019-0282-7

    authors: Price DB,Bosnic-Anticevich S,Pavord ID,Roche N,Halpin DMG,Bjermer L,Usmani OS,Brusselle G,Ming SWY,Rastogi S

    更新日期:2019-08-21 00:00:00

  • Fungal and bacterial microbiome dysbiosis and imbalance of trans-kingdom network in asthma.

    abstract:Background:Fungal and bacterial microbiota play an important role in development of asthma. We aim to characterize airway microbiome (mycobiome, bacteriome) and functional genes in asthmatics and controls. Methods:Sputum microbiome of controls, untreated asthma patients and inhaled corticosteroid (ICS) receiving patie...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/s13601-020-00345-8

    authors: Huang C,Yu Y,Du W,Liu Y,Dai R,Tang W,Wang P,Zhang C,Shi G

    更新日期:2020-10-22 00:00:00

  • A meta-analysis of baseline characteristics in trials on mite allergen avoidance in asthmatics: room for improvement.

    abstract:Background:Evidence regarding the clinical effectiveness of mite allergen avoidance for the treatment of asthma is lacking. In previous meta-analyses on mite allergen control, the baseline data were not discussed in detail. This study updates and extends the existing Cochrane review by Gøtzsche and Johansen (Cochrane D...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章,评审

    doi:10.1186/s13601-019-0306-3

    authors: van Boven FE,de Jong NW,Braunstahl GJ,Gerth van Wijk R,Arends LR

    更新日期:2020-01-06 00:00:00

  • Prospective validation of the "rhino conjunctivitis allergy-control-SCORE©" (RC-ACS©).

    abstract:BACKGROUND:Recently we reported the validation of the "Allergy-Control-SCORE© (ACS)" which assesses symptom severity as well as medication use on three dimensions lung, nose and eyes. The aim of this study was to test the validity of the score for eyes and nose. METHODS:One-hundred-twenty-one consenting subjects (age ...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/2045-7022-2-17

    authors: Häfner D,Reich K,Zschocke I,Lotzin A,Meyer H,Kettner J,Narkus A

    更新日期:2012-09-19 00:00:00

  • Cosmetics for neonates and infants: haptens in products' composition.

    abstract::Cosmetics and skin care products for neonates and infants are considered as ''hypoallergenic'', "tested" or ''safe''. Nevertheless, the prevalence of haptens in these products is a matter of concern, since allergic contact dermatitis in children is gaining an importance. We aimed to assess the prevalence of haptens in...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/s13601-019-0257-8

    authors: Dumycz K,Kunkiel K,Feleszko W

    更新日期:2019-03-08 00:00:00

  • Allergen immunotherapy for IgE-mediated food allergy: protocol for a systematic review.

    abstract:BACKGROUND:The European Academy of Allergy and Clinical Immunology (EAACI) is in the process of developing the EAACI Guidelines for Allergen Immunotherapy (AIT) for IgE-mediated food allergy. We seek to critically assess the effectiveness, cost-effectiveness and safety of AIT in IgE-mediated food allergy. METHODS:We w...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/s13601-016-0113-z

    authors: Dhami S,Nurmatov U,Pajno GB,Fernandez-Rivas M,Muraro A,Roberts G,Akdis C,Alvaro-Lozano M,Beyer K,Bindslev-Jensen C,Burks W,du Toit G,Ebisawa M,Eigenmann P,Knol E,Makela M,Nadeau KC,O'Mahony L,Papadopoulos N,Poulsen

    更新日期:2016-07-05 00:00:00

  • Eosinophilic disorders: evaluation of current classification and diagnostic criteria, proposal of a practical diagnostic algorithm.

    abstract::Eosinophilic disorders represent a group of pathologic conditions with highly heterogeneous pathophysiology and clinical presentation and variable prognosis, ranging from asymptomatic or mild, to severe and complex cases, with fatal outcome. Interest in this group of disorders has increased during the last two decades...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章,评审

    doi:10.1186/s13601-019-0277-4

    authors: Leru PM

    更新日期:2019-07-25 00:00:00

  • Mapping hypersensitivity/allergic diseases in the International Classification of Diseases (ICD)-11: cross-linking terms and unmet needs.

    abstract:BACKGROUND:With the aim of actively contributing to the ongoing 11(th) International Classification of Diseases (ICD) revision, an international collaboration led by the European Academy of Allergy and Clinical Immunology (EAACI) has decided to revise the classification of hypersensitivity/allergic diseases and to vali...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/s13601-015-0063-x

    authors: Tanno LK,Calderon M,Papadopoulos NG,Demoly P,EAACI\/WAO Task force of a Global Classification of Hypersensitivity\/Allergic diseases.

    更新日期:2015-06-03 00:00:00

  • Categorization of allergic disorders in the new World Health Organization International Classification of Diseases.

    abstract:BACKGROUND:Although efforts to improve the classification of hypersensitivity/allergic diseases have been made, they have not been considered a top-level category in the International Classification of Diseases (ICD)-10 and still are not in the ICD-11 beta phase linearization. ICD-10 is the most used classification sys...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/2045-7022-4-42

    authors: Tanno LK,Calderon MA,Goldberg BJ,Akdis CA,Papadopoulos NG,Demoly P

    更新日期:2014-11-28 00:00:00

  • Indoleamine 2,3-dioxygenase expression in patients with allergic rhinitis: a case-control study.

    abstract:BACKGROUND:Indoleamine 2,3-dioxygenase (IDO) is a tryptophan catalyzing enzyme. It has been suggested that it has a role in lower airway allergic inflammations, but its role in allergic rhinitis has not been investigated. OBJECTIVE:Our aim was to evaluate the expression of IDO in the nasal mucosa of allergic rhinitis ...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/2045-7022-1-17

    authors: Luukkainen A,Karjalainen J,Honkanen T,Lehtonen M,Paavonen T,Toppila-Salmi S

    更新日期:2011-12-12 00:00:00

  • Prolonged efficacy of the 300IR 5-grass pollen tablet up to 2 years after treatment cessation, as measured by a recommended daily combined score.

    abstract:BACKGROUND:The 300IR (index of reactivity) 5-grass pollen tablet has favorable short-term and sustained clinical efficacy in patients with grass pollen-induced allergic rhinoconjunctivitis (ARC). Here, we report maintenance of efficacy and safety over 2 years following treatment discontinuation. METHODS:Randomized, do...

    journal_title:Clinical and translational allergy

    pub_type: 杂志文章

    doi:10.1186/s13601-015-0057-8

    authors: Didier A,Malling HJ,Worm M,Horak F,Sussman GL

    更新日期:2015-05-22 00:00:00