Increased expression of ICAM-1, VCAM-1, MCP-1, and MIP-1 alpha by spinal perivascular macrophages during experimental allergic encephalomyelitis in rats.


BACKGROUND:T-cells extravasation and CNS parenchyma infiltration during autoimmune neurodegenerative disease can be evoked by local antigen presenting cells. Studying the chemoattracting potential of spinal perivascular macrophages (SPM) during experimental allergic encephalomyelitis (EAE), we observed numerous infiltrates of densely-packed mononuclear cells. Apart from the poor spatial and optical resolution, no differentiation between the resident SPM (mabs ED1+, ED2+) and the just recruited monocytes/macrophages (mab ED1+) was possible. RESULTS:This is why we labeled SPM by injections of different fluoresecent dyes into the lateral cerebral ventricle before induction of active EAE. Within an additional experimental set EAE was induced by an intraperitoneal injection of T-cells specifically sensitized to myelin basic protein (MBP) and engineered to express the green fluorescent protein (GFP). In both experiments we observed a strong activation of SPM (mabs OX6+, SILK6+, CD40+, CD80+, CD86+) which was accompanied by a consistently increased expression of ICAM-1, VCAM-1, and the chemokines MCP-1 and MIP-1alpha. CONCLUSION:These observations indicate that SPM play a role in promoting lymphocyte extravasation.


BMC Immunol


BMC immunology


Hofmann N,Lachnit N,Streppel M,Witter B,Neiss WF,Guntinas-Lichius O,Angelov DN





Has Abstract


2002-08-26 00:00:00








  • Multifaceted effects of synthetic TLR2 ligand and Legionella pneumophilia on Treg-mediated suppression of T cell activation.

    abstract:BACKGROUND:Regulatory T cells (Treg) play a crucial role in maintaining immune homeostasis and self-tolerance. The immune suppressive effects of Tregs should however be limited in case effective immunity is required against pathogens or cancer cells. We previously found that the Toll-like receptor 2 (TLR2) agonist, Pam...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: van Maren WW,Nierkens S,Toonen LW,Bolscher JM,Sutmuller RP,Adema GJ

    更新日期:2011-03-24 00:00:00

  • Low-dose plasmid DNA treatment increases plasma vasopressin and regulates blood pressure in experimental endotoxemia.

    abstract:BACKGROUND:Although plasmid DNA encoding an antigen from pathogens or tumor cells has been widely studied as vaccine, the use of plasmid vector (without insert) as therapeutic agent requires further investigation. RESULTS:Here, we showed that plasmid DNA (pcDNA3) at low doses inhibits the production of IL-6 and TNF-α ...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Malardo T,Batalhão ME,Panunto-Castelo A,Almeida LP,Padilha E,Fontoura IC,Silva CL,Carnio EC,Coelho-Castelo AA

    更新日期:2012-11-08 00:00:00

  • Severe anaphylactic reactions to glutamic acid decarboxylase (GAD) self peptides in NOD mice that spontaneously develop autoimmune type 1 diabetes mellitus.

    abstract:BACKGROUND:Insulin dependent (i.e., "type 1") diabetes mellitus (T1DM) is considered to be a T cell mediated disease in which TH1 and Tc autoreactive cells attack the pancreatic islets. Among the beta-cell antigens implicated in T1DM, glutamic acid decarboxylase (GAD) 65 appears to play a key role in the development of...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Pedotti R,Sanna M,Tsai M,DeVoss J,Steinman L,McDevitt H,Galli SJ

    更新日期:2003-02-22 00:00:00

  • Efficient isolation of highly purified tonsil B lymphocytes using RosetteSep with allogeneic human red blood cells.

    abstract:BACKGROUND:Human tonsils are a rich source of B lymphocytes exhibiting a variety of phenotypes and activation states. Existing methods of purification are time consuming or costly. The aim of the present study was to optimize conditions to isolate large numbers of highly purified primary B lymphocytes from tonsils in a...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Zuccolo J,Unruh TL,Deans JP

    更新日期:2009-05-27 00:00:00

  • Streptococcus pneumoniae synergizes with nontypeable Haemophilus influenzae to induce inflammation via upregulating TLR2.

    abstract:BACKGROUND:Toll-like receptor 2 (TLR2) plays a critical role in mediating inflammatory/immune responses against bacterial pathogens in lung. Streptococcus pneumoniae (S. pneumoniae) and nontypeable Haemophilus influenzae (NTHi) were previously reported to synergize with each other to induce inflammatory responses. Desp...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Lim JH,Ha U,Sakai A,Woo CH,Kweon SM,Xu H,Li JD

    更新日期:2008-07-29 00:00:00

  • Co-inherited mutations of Fas and caspase-10 in development of the autoimmune lymphoproliferative syndrome.

    abstract:BACKGROUND:Autoimmune lymphoproliferative syndrome (ALPS) is a rare inherited disorder characterized by defective function of Fas, autoimmune manifestations that predominantly involve blood cells, polyclonal accumulation of lymphocytes in the spleen and lymph nodes with lymphoadenomegaly and/or splenomegaly, and expans...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Cerutti E,Campagnoli MF,Ferretti M,Garelli E,Crescenzio N,Rosolen A,Chiocchetti A,Lenardo MJ,Ramenghi U,Dianzani U

    更新日期:2007-11-13 00:00:00

  • Secretion of celiac disease autoantibodies after in vitro gliadin challenge is dependent on small-bowel mucosal transglutaminase 2-specific IgA deposits.

    abstract:BACKGROUND:In celiac disease gluten, the disease-inducing toxic component in wheat, induces the secretion of autoantibodies which are targeted against transglutaminase 2 (TG2). These autoantibodies are produced in the small-intestinal mucosa, where they can be found deposited extracellularly below the epithelial baseme...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Stenman SM,Lindfors K,Korponay-Szabo IR,Lohi O,Saavalainen P,Partanen J,Haimila K,Wieser H,Mäki M,Kaukinen K

    更新日期:2008-02-29 00:00:00

  • Tumor necrosis factor-alpha induced expression of matrix metalloproteinase-9 through p21-activated kinase-1.

    abstract:BACKGROUND:Expressed in embryonic development, matrix metalloprotein-9 (MMP-9) is absent in most of developed adult tissues, but recurs in inflammation during tissue injury, wound healing, tumor formation and metastasis. Expression of MMP-9 is tightly controlled by extracellular cues including pro-inflammatory cytokine...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Zhou L,Yan C,Gieling RG,Kida Y,Garner W,Li W,Han YP

    更新日期:2009-03-19 00:00:00

  • Potential T cell epitopes of Mycobacterium tuberculosis that can instigate molecular mimicry against host: implications in autoimmune pathogenesis.

    abstract:BACKGROUND:Molecular mimicry between microbial antigens and host-proteins is one of the etiological enigmas for the occurrence of autoimmune diseases. T cells that recognize cross-reactive epitopes may trigger autoimmune reactions. Intriguingly, autoimmune diseases have been reported to be prevalent in tuberculosis end...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Chodisetti SB,Rai PK,Gowthaman U,Pahari S,Agrewala JN

    更新日期:2012-03-21 00:00:00

  • Ten-color flow cytometry reveals distinct patterns of expression of CD124 and CD126 by developing thymocytes.

    abstract:BACKGROUND:We have developed a 12-parameter/10-color flow cytometric staining method for the simultaneous detection and characterization of 21 mouse thymocyte subpopulations that represent discreet stages of T cell development. To demonstrate the utility of this method, we assessed cytokine receptor expression on mouse...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Tan C,Taylor AA,Coburn MZ,Marino JH,Van De Wiele CJ,Teague TK

    更新日期:2011-06-20 00:00:00

  • Tolerance induced via TLR2 and TLR4 in human dendritic cells: role of IRAK-1.

    abstract:BACKGROUND:While dendritic cells (DCs) can induce tolerance in T cells, little is known about tolerance induction in DCs themselves. We have analysed tolerance induced in human in-vitro generated DCs by repeated stimulation with ligands for TLR4 and TLR2. RESULTS:DCs stimulated with the TLR4 ligand LPS did show a rapi...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Albrecht V,Hofer TP,Foxwell B,Frankenberger M,Ziegler-Heitbrock L

    更新日期:2008-11-24 00:00:00

  • The DNA methyltransferase inhibitor, guadecitabine, targets tumor-induced myelopoiesis and recovers T cell activity to slow tumor growth in combination with adoptive immunotherapy in a mouse model of breast cancer.

    abstract:BACKGROUND:Myeloid derived suppressor cells (MDSCs) present a significant obstacle to cancer immunotherapy because they dampen anti-tumor cytotoxic T cell responses. Previous groups, including our own, have reported on the myelo-depletive effects of certain chemotherapy agents. We have shown previously that decitabine ...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Luker AJ,Graham LJ,Smith TM Jr,Camarena C,Zellner MP,Gilmer JS,Damle SR,Conrad DH,Bear HD,Martin RK

    更新日期:2020-02-27 00:00:00

  • In vitro and in vivo protective efficacies of antibodies that neutralize the RNA N-glycosidase activity of Shiga toxin 2.

    abstract:BACKGROUND:Shiga toxin 2 (Stx2), one of two Stx liberated by Stx-producing Escherichia coli, is composed of an A subunit monomer and a B subunit pentamer, and is directly linked with hemolytic uremic syndrome in children. The pentameric B subunit binds to its cell surface receptor Gb3 for toxin internalization, and the...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Jeong KI,Chapman-Bonofiglio S,Singh P,Lee J,Tzipori S,Sheoran AS

    更新日期:2010-03-24 00:00:00

  • Mesenchymal stromal (stem) cells suppress pro-inflammatory cytokine production but fail to improve survival in experimental staphylococcal toxic shock syndrome.

    abstract:BACKGROUND:Toxic shock syndrome (TSS) is caused by an overwhelming host-mediated response to bacterial superantigens produced mainly by Staphylococcus aureus and Streptococcus pyogenes. TSS is characterized by aberrant activation of T cells and excessive release of pro-inflammatory cytokines ultimately resulting in cap...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Kim H,Darwish I,Monroy MF,Prockop DJ,Liles WC,Kain KC

    更新日期:2014-01-14 00:00:00

  • Fermented fish oil suppresses T helper 1/2 cell response in a mouse model of atopic dermatitis via generation of CD4+CD25+Foxp3+ T cells.

    abstract:BACKGROUND:Allergic skin inflammation such as atopic dermatitis (AD), which is characterized by pruritus and inflammation, is regulated partly through the activity of regulatory T cells (Tregs). Tregs play key roles in the immune response by preventing or suppressing the differentiation, proliferation and function of v...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Han SC,Kang GJ,Ko YJ,Kang HK,Moon SW,Ann YS,Yoo ES

    更新日期:2012-08-09 00:00:00

  • Efficient adenovirus-mediated gene transfer into primary T cells and thymocytes in a new coxsackie/adenovirus receptor transgenic model.

    abstract:BACKGROUND:Gene transfer studies in primary T cells have suffered from the limitations of conventional viral transduction or transfection techniques. Replication-defective adenoviral vectors are an attractive alternative for gene delivery. However, naive lymphocytes are not readily susceptible to infection with adenovi...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Hurez V,Dzialo-Hatton R,Oliver J,Matthews RJ,Weaver CT

    更新日期:2002-05-02 00:00:00

  • Limiting prolonged inflammation during proliferation and remodeling phases of wound healing in streptozotocin-induced diabetic rats supplemented with camel undenatured whey protein.

    abstract:BACKGROUND:Impaired diabetic wound healing occurs as a consequence of excessive reactive oxygen species (ROS) and inflammatory cytokine production. We previously found that whey protein (WP) was able to normally regulate the ROS and inflammatory cytokines during the inflammatory phase (first day) in streptozotocin (STZ...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Ebaid H,Ahmed OM,Mahmoud AM,Ahmed RR

    更新日期:2013-07-25 00:00:00

  • Investigations of immunogenic, allergenic and adjuvant properties of Cry1Ab protein after intragastric exposure in a food allergy model in mice.

    abstract:BACKGROUND:In genetically modified (GM) crops there is a risk that the inserted genes may introduce new allergens and/or adjuvants into the food and feed chain. The MON810 maize, expressing the insecticidal Cry1Ab toxin, is grown in many countries worldwide. In animal models, intranasal and intraperitoneal immunisation...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Andreassen M,Bøhn T,Wikmark OG,Bodin J,Traavik T,Løvik M,Nygaard UC

    更新日期:2016-05-04 00:00:00

  • Association of proinflammatory genes expression with serum interleukin 1β and free fatty acids in metabolically healthy and unhealthy abdominally obese individuals: a case-control study.

    abstract:BACKGROUND:Proinflammatory genes are highly expressed in several metabolic disorders associated with obesity. But it is not clarified whether gene expression levels and downstream inflammatory markers are related to the metabolic state or the presence of obesity. Hence, the present study aimed to compare Toll-Like Rece...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Amiri P,Baradaran B,Saghafi-Asl M,Naghizadeh M,Shanehbandi D,Karamzad N,Vahed SZ

    更新日期:2019-07-04 00:00:00

  • NK cell predicts the severity of acute graft-versus-host disease in patients after allogeneic stem cell transplantation using antithymocyte globulin (ATG) in pretreatment scheme.

    abstract:BACKGROUND:Graft-versus-host disease (GVHD) is one of the most complex complications after allogeneic stem cell transplantation. Current standard of grading system is based on clinical symptoms in skin, liver and intestinal. However, it's difficult to differ GVHD and its extent just by clinical manifestation. Here we r...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Zhang P,Yang S,Zou Y,Yan X,Wu H,Zhou M,Sun YC,Zhang Y,Zhu H,Xu K,Wang Y,Sheng LX,Mu Q,Sun L,Ouyang G

    更新日期:2019-12-09 00:00:00

  • The innate interferon gamma response of BALB/c and C57BL/6 mice to in vitro Burkholderia pseudomallei infection.

    abstract:BACKGROUND:Burkholderia pseudomallei is the causative agent for melioidosis. For many bacterial infections, cytokine dysregulation is one of the contributing factors to the severe clinical outcomes in the susceptible hosts. The C57BL/6 and BALB/c mice have been established as a differential model of susceptibility in m...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Koo GC,Gan YH

    更新日期:2006-08-18 00:00:00

  • Immunome database for marsupials and monotremes.

    abstract:BACKGROUND:To understand the evolutionary origins of our own immune system, we need to characterise the immune system of our distant relatives, the marsupials and monotremes. The recent sequencing of the genomes of two marsupials (opossum and tammar wallaby) and a monotreme (platypus) provides an opportunity to charact...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Wong ES,Papenfuss AT,Belov K

    更新日期:2011-08-19 00:00:00

  • Evaluation of signal transduction pathways after transient cutaneous adenoviral gene delivery.

    abstract:BACKGROUND:Adenoviral vectors have provided effective methods for in vivo gene delivery in therapeutic applications. However, these vectors can induce immune responses that may severely affect the ability of vector re-application. There is limited information about the mechanisms and signal transduction pathways involv...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Steinstraesser L,Sorkin M,Jacobsen F,Al-Benna S,Kesting MR,Niederbichler AD,Otte JM,Hirsch T,Stupka J,Steinau HU,Schulte M

    更新日期:2011-01-21 00:00:00

  • Effects of F/G-actin ratio and actin turn-over rate on NADPH oxidase activity in microglia.

    abstract:BACKGROUND:Most in vivo studies that have addressed the role of actin dynamics in NADPH oxidase function in phagocytes have used toxins to modulate the polymerization state of actin and mostly effects on actin has been evaluated by end point measurements of filamentous actin, which says little about actin dynamics, and...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Rasmussen I,Pedersen LH,Byg L,Suzuki K,Sumimoto H,Vilhardt F

    更新日期:2010-09-08 00:00:00

  • CD16 expression on neutrophils predicts treatment efficacy of capecitabine in colorectal cancer patients.

    abstract:BACKGROUND:Early detection of capecitabine-resistance could largely increase overall survival of colorectal cancer (CRC) patients. Previous studies suggested examination of immune cells in peripheral blood would help to predict efficacy of chemotherapy. METHODS:We examined the immunological characteristics of peripher...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Lu Y,Huang Y,Huang L,Xu Y,Wang Z,Li H,Zhang T,Zhong M,Gao WQ,Zhang Y

    更新日期:2020-08-08 00:00:00

  • Characteristics and reference ranges of CD4+T cell subpopulations among healthy adult Han Chinese in Shanxi Province, North China.

    abstract:BACKGROUND:Immunophenotyping of blood lymphocytes is an essential tool to evaluate the immune function of patients with immunodeficiency or autoimmunity. Predominately identified CD4+T cell subsets, Th1, Th2, Th17, as well as regulatory T (Treg) cells, play crucial roles in several immunological and pathological condit...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Niu HQ,Zhao XC,Li W,Xie JF,Liu XQ,Luo J,Zhao WP,Li XF

    更新日期:2020-08-03 00:00:00

  • Subsets of mononuclear phagocytes are enriched in the inflamed colons of patients with IBD.

    abstract:BACKGROUND:Myeloid cells, especially mononuclear phagocytes, which include monocytes, macrophages and dendritic cells (DC), play vital roles in innate immunity, and in the initiation and maintenance of adaptive immunity. While T cell-associated activation pathways and cytokines have been identified and evaluated in inf...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Liu H,Dasgupta S,Fu Y,Bailey B,Roy C,Lightcap E,Faustin B

    更新日期:2019-11-12 00:00:00

  • Rheumatoid factor is correlated with disease activity and inflammatory markers in antineutrophil cytoplasmic antibody-associated vasculitis.

    abstract:BACKGROUND:Some patients with antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) also have positivity of rheumatoid factor (RF). However, the clinical significance of this occurrence remains unknown in AAV patients. The aim of this study was to clarify an association between the presence of RF and c...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Watanabe S,Gono T,Nishina K,Sugitani N,Watanabe E,Yabe H,Terai C

    更新日期:2017-12-20 00:00:00

  • Combined TLR2 and TLR4 ligation in the context of bacterial or helminth extracts in human monocyte derived dendritic cells: molecular correlates for Th1/Th2 polarization.

    abstract:BACKGROUND:Recognition of pathogens by dendritic cells (DCs) through interaction with pattern recognition receptors, including Toll like receptors (TLR), is crucial for the initiation of appropriate polarized T helper (Th) cell responses. Yet, the characteristics and differences in molecular profiles of DCs with differ...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: van Riet E,Everts B,Retra K,Phylipsen M,van Hellemond JJ,Tielens AG,van der Kleij D,Hartgers FC,Yazdanbakhsh M

    更新日期:2009-02-04 00:00:00

  • Bacillus anthracis' lethal toxin induces broad transcriptional responses in human peripheral monocytes.

    abstract:BACKGROUND:Anthrax lethal toxin (LT), produced by the Gram-positive bacterium Bacillus anthracis, is a highly effective zinc dependent metalloprotease that cleaves the N-terminus of mitogen-activated protein kinase kinases (MAPKK or MEKs) and is known to play a role in impairing the host immune system during an inhalat...

    journal_title:BMC immunology

    pub_type: 杂志文章


    authors: Chauncey KM,Lopez MC,Sidhu G,Szarowicz SE,Baker HV,Quinn C,Southwick FS

    更新日期:2012-07-02 00:00:00