Abstract：
:Hyperglycaemia-induced neurotoxicity involved in the pathogenesis of diabetic encephalopathy and neuronal senescence is one of the worst effects of hyperglyceamic neurotoxicity. Cannabinoid receptor type 1 (CB1) has neuroprotective function in a series of neuropathy. Spermidine (Spd) has anti-aging function in many tissues. However, the role of Spd in hyperglyceamia-induced neuronal senescence remains unexplored. Therefore, we used high glucose (HG)-treated HT-22 cell as vitro model to investigate whether Spd protects neurons against hyperglyceamia-induced senescence and the mediatory role of CB1 receptor. The HT-22 cells were cultured in HG condition in the presence of different dose of Spd. Then, the viability of cells was measured by Cell Counting Kit-8 (CCK-8) assay. The senescence of cells was detected by Senescence-associated β-galactosidase (SA-β-Gal) staining. The expressions of p16INK4a , p21CIP1 and CB1 receptor were measured by western blot. We found that Spd inhibited HG-induced neurotoxicity (the loss of cell viability) and senescence (the increase of SA-β-Gal positive cells, the upregulation of p16INK4a and p21CIP1 ) in HT-22 cells. Also, Spd prevented HG-induced downregulation of CB1 receptor in HT-22 cells. Furthermore, we demonstrated that AM251 (a specific inhibitor of the CB1 receptor) reversed the protective effects of Spd on HG-induced neurotoxicity and senescence. These results indicated that Spd prevents HG-induced neurotoxicity and senescence via the upregulation of CB1 receptor. Our findings provide a promising future of Spd-based preventions and therapies for diabetic encephalopathy.
journal_name
Clin Exp Pharmacol Physioljournal_title
Clinical and experimental pharmacology & physiologyauthors
Zhu WW,Xiao F,Tang YY,Zou W,Li X,Zhang P,Wang AP,Tang XQdoi
10.1111/1440-1681.12955subject
Has Abstractpub_date
2018-08-01 00:00:00pages
832-840issue
8eissn
0305-1870issn
1440-1681journal_volume
45pub_type
杂志文章abstract：:This study investigates the effect of chronic consumption of a high-fat diet rich in corn oil (CO-HFD) on atrial cells ultrastructure, antioxidant levels and markers of intrinsic cell death of both control and type 1 diabetes mellitus (T1DM)-induced rats. Adult male rats (10 rats/group) were divided into four groups: ...
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doi：10.1111/j.1440-1681.1986.tb00313.x
更新日期：1986-01-01 00:00:00
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doi：10.1111/1440-1681.13274
更新日期：2020-06-01 00:00:00
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更新日期：1989-04-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2008-01-01 00:00:00
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doi：10.1111/j.1440-1681.1987.tb02420.x
更新日期：1987-11-01 00:00:00
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journal_title：Clinical and experimental pharmacology & physiology
pub_type： 杂志文章
doi：10.1111/j.1440-1681.1986.tb00946.x
更新日期：1986-08-01 00:00:00
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更新日期：2010-03-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2007-10-01 00:00:00
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doi：10.1111/j.1440-1681.1980.tb00084.x
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更新日期：1988-04-01 00:00:00
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更新日期：1996-08-01 00:00:00
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更新日期：2008-01-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：1996-04-01 00:00:00
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doi：10.1111/j.1440-1681.1989.tb01614.x
更新日期：1989-08-01 00:00:00
abstract：:1. Ethionine administered acutely to the adult female rat markedly elevates and then lowers plasma iron concentration over several days. Liver iron undergoes a reverse cycle. 2. Ethionine does not cause changes in the blood parameters, including total plasma iron-binding capacity and plasma iron clearance. Erythrocyte...
journal_title：Clinical and experimental pharmacology & physiology
pub_type： 杂志文章
doi：10.1111/j.1440-1681.1975.tb01851.x
更新日期：1975-11-01 00:00:00
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journal_title：Clinical and experimental pharmacology & physiology
pub_type： 杂志文章
doi：10.1111/j.1440-1681.2009.05291.x
更新日期：2010-02-01 00:00:00
abstract：:1. The field of extracellular nucleotides and purinoceptors has undergone a resurgence of interest and enthusiasm in the past decade. More and more investigators are probing the physiological and pathophysiological roles of P2 receptors in virtually every organ system, including the kidney. 2. With this renewed intere...
journal_title：Clinical and experimental pharmacology & physiology
pub_type： 杂志文章,评审
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更新日期：2001-04-01 00:00:00
abstract：:In the context of the ongoing debate on the mechanism of blood pressure (BP) regulation and pathophysiology of arterial hypertension ("renocentric" vs "neural" concepts), attention is focused on the putative regulatory role of changes in renal medullary blood flow (MBF). Experimental evidence is analysed with regard t...
journal_title：Clinical and experimental pharmacology & physiology
pub_type： 杂志文章,评审
doi：10.1111/1440-1681.13303
更新日期：2020-08-01 00:00:00
