Finding the needle in the haystack: why high-throughput screening is good for your health.


:High-throughput screening is an essential component of the toolbox of modern technologies that improve speed and efficiency in contemporary cancer drug development. This is particularly important as we seek to exploit, for maximum therapeutic benefit, the large number of new molecular targets emerging from the Human Genome Project and cancer genomics. Screening of diverse collections of low molecular weight compounds plays a key role in providing chemical starting points for iterative optimisation by medicinal chemistry. Examples of successful drug discovery programmes based on high-throughput screening are described, and these offer potential in the treatment of breast cancer and other malignancies.


Breast Cancer Res


Aherne GW,McDonald E,Workman P





Has Abstract


2002-01-01 00:00:00












  • Comparison of percent density from raw and processed full-field digital mammography data.

    abstract:INTRODUCTION:Mammographic density has been established as a strong risk factor for breast cancer, primarily using digitized film mammograms. Full-field digital mammography (FFDM) is replacing film mammography, has different properties than film, and provides both raw and processed clinical display representation images...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Vachon CM,Fowler EE,Tiffenberg G,Scott CG,Pankratz VS,Sellers TA,Heine JJ

    更新日期:2013-01-04 00:00:00

  • Vaccinia virus GLV-1h153 is a novel agent for detection and effective local control of positive surgical margins for breast cancer.

    abstract:INTRODUCTION:Surgery is currently the definitive treatment for early-stage breast cancer. However, the rate of positive surgical margins remains unacceptably high. The human sodium iodide symporter (hNIS) is a naturally occurring protein in human thyroid tissue, which enables cells to concentrate radionuclides. The hNI...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Gholami S,Chen CH,Belin LJ,Lou E,Fujisawa S,Antonacci C,Carew A,Chen NG,De Brot M,Zanzonico PB,Szalay AA,Fong Y

    更新日期:2013-03-18 00:00:00

  • Breast cancer in association with thyroid disorders.

    abstract:BACKGROUND:The relationship between breast cancer and thyroid diseases is controversial. Discrepant results have been reported in the literature. The incidences of autoimmune and nonautoimmune thyroid diseases were investigated in patients with breast cancer and age-matched control individuals without breast or thyroid...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Turken O,NarIn Y,DemIrbas S,Onde ME,Sayan O,KandemIr EG,YaylacI M,Ozturk A

    更新日期:2003-01-01 00:00:00

  • Estrogen regulation of mammary gland development and breast cancer: amphiregulin takes center stage.

    abstract::Estrogen-mediated proliferation is fundamental to normal mammary gland development. Recent studies have demonstrated that amphiregulin is a critical paracrine regulator of estrogen action during ductal morphogenesis. These studies implicate a critical role for amphiregulin in mammary stem cell differentiation as well ...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章,评审


    authors: LaMarca HL,Rosen JM

    更新日期:2007-01-01 00:00:00

  • Interaction with adipocyte stromal cells induces breast cancer malignancy via S100A7 upregulation in breast cancer microenvironment.

    abstract:BACKGROUND:Breast adipocytes play important roles in both the development and function of mammary epithelial cells. Therefore, carcinoma-adipose stromal cell (ASC) interactions have been considered pivotal in supporting tumor growth in breast cancer. In addition, it has been demonstrated that the biological features of...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Sakurai M,Miki Y,Takagi K,Suzuki T,Ishida T,Ohuchi N,Sasano H

    更新日期:2017-06-19 00:00:00

  • Functional variants at the 21q22.3 locus involved in breast cancer progression identified by screening of genome-wide estrogen response elements.

    abstract:INTRODUCTION:Estrogen forms a complex with the estrogen receptor (ER) that binds to estrogen response elements (EREs) in the regulatory region of estrogen-responsive genes and regulates their transcription. Sequence variants in the regulatory regions have the potential to affect the transcription factor-regulatory sequ...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Hsiung CN,Chu HW,Huang YL,Chou WC,Hu LY,Hsu HM,Wu PE,Hou MF,Yu JC,Shen CY

    更新日期:2014-10-09 00:00:00

  • Treating breast cancer through novel inhibitors of the phosphatidylinositol 3'-kinase pathway.

    abstract::Recent studies indicate that constitutive signaling through the phosphatidylinositol 3'-kinase (PI3K) pathway is a cause of treatment resistance in breast cancer patients. This implies that patients with tumors that exhibit aberrant PI3K signaling may benefit from targeted pathway inhibitors. The first agents to make ...

    journal_title:Breast cancer research : BCR

    pub_type: 评论,杂志文章


    authors: Crowder RJ,Ellis MJ

    更新日期:2005-01-01 00:00:00

  • Tumor-specific HMG-CoA reductase expression in primary premenopausal breast cancer predicts response to tamoxifen.

    abstract:INTRODUCTION:We previously reported an association between tumor-specific 3-hydroxy-3-methylglutharyl-coenzyme A reductase (HMG-CoAR) expression and a good prognosis in breast cancer. Here, the predictive value of HMG-CoAR expression in relation to tamoxifen response was examined. METHODS:HMG-CoAR protein and RNA expr...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Brennan DJ,Laursen H,O'Connor DP,Borgquist S,Uhlen M,Gallagher WM,Pontén F,Millikan RC,Rydén L,Jirström K

    更新日期:2011-01-31 00:00:00

  • Increased genomic burden of germline copy number variants is associated with early onset breast cancer: Australian breast cancer family registry.

    abstract:BACKGROUND:Women with breast cancer who have multiple affected relatives are more likely to have inherited genetic risk factors for the disease. All the currently known genetic risk factors for breast cancer account for less than half of the average familial risk. Furthermore, the genetic factor(s) underlying an increa...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Walker LC,Pearson JF,Wiggins GA,Giles GG,Hopper JL,Southey MC

    更新日期:2017-03-16 00:00:00

  • Polyclonal HER2-specific antibodies induced by vaccination mediate receptor internalization and degradation in tumor cells.

    abstract:INTRODUCTION:Sustained HER2 signaling at the cell surface is an oncogenic mechanism in a significant proportion of breast cancers. While clinically effective therapies targeting HER2 such as mAbs and tyrosine kinase inhibitors exist, tumors overexpressing HER2 eventually progress despite treatment. Thus, abrogation of ...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Ren XR,Wei J,Lei G,Wang J,Lu J,Xia W,Spector N,Barak LS,Clay TM,Osada T,Hamilton E,Blackwell K,Hobeika AC,Morse MA,Lyerly HK,Chen W

    更新日期:2012-06-07 00:00:00

  • Cooperative signaling between Wnt1 and integrin-linked kinase induces accelerated breast tumor development.

    abstract:INTRODUCTION:Breast cancer is genetically and clinically a heterogeneous disease. However, the exact contribution of different cell types and oncogenic mutations to this heterogeneity are not well understood. Recently, we discovered an interaction between Wnt and integrin-linked kinase (ILK) within the signaling cascad...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Oloumi A,Maidan M,Lock FE,Tearle H,McKinney S,Muller WJ,Aparicio SA,Dedhar S

    更新日期:2010-01-01 00:00:00

  • Approaches towards expression profiling the response to treatment.

    abstract::Over the past 8 years there has been a wealth of breast cancer gene expression studies. The majority of these studies have focused upon characterising a tumour at presentation, before treatment, rather than looking at the effects of treatment on the tumour. More recently, a number of groups have moved from predicting ...

    journal_title:Breast cancer research : BCR

    pub_type: 评论,社论


    authors: Sims AH,Bartlett JM

    更新日期:2008-01-01 00:00:00

  • Role of ADAM17 in the non-cell autonomous effects of oncogene-induced senescence.

    abstract:INTRODUCTION:Cellular senescence is a terminal cell proliferation arrest that can be triggered by oncogenes. One of the traits of oncogene-induced senescence (OIS) is the so-called senescence-associated secretory phenotype or senescence secretome. Depending on the context, the non-cell autonomous effects of OIS may var...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Morancho B,Martínez-Barriocanal Á,Villanueva J,Arribas J

    更新日期:2015-08-12 00:00:00

  • RAC1 GTPase plays an important role in γ-irradiation induced G2/M checkpoint activation.

    abstract:INTRODUCTION:In response to gamma-irradiation (IR)-induced double-strand DNA breaks, cells undergo cell-cycle arrest, allowing time for DNA repair before reentering the cell cycle. G2/M checkpoint activation involves activation of ataxia telangiectasia mutated (ATM)/ATM- and rad3-related (ATR) kinases and inhibition of...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Yan Y,Greer PM,Cao PT,Kolb RH,Cowan KH

    更新日期:2012-04-11 00:00:00

  • JMJD2A contributes to breast cancer progression through transcriptional repression of the tumor suppressor ARHI.

    abstract:INTRODUCTION:Breast cancer is a worldwide health problem and the leading cause of cancer death among females. We previously identified Jumonji domain containing 2A (JMJD2A) as a critical mediator of breast cancer proliferation, migration and invasion. We now report that JMJD2A could promote breast cancer progression th...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Li LL,Xue AM,Li BX,Shen YW,Li YH,Luo CL,Zhang MC,Jiang JQ,Xu ZD,Xie JH,Zhao ZQ

    更新日期:2014-05-30 00:00:00

  • DACT1, an antagonist to Wnt/β-catenin signaling, suppresses tumor cell growth and is frequently silenced in breast cancer.

    abstract:INTRODUCTION:Aberrant activation of Wnt/β-catenin signaling plays an important role in the pathogenesis of breast cancer. DACT1 (Dapper/Frodo) has been identified as involved in antagonizing Wnt/β-catenin signaling through interacting with Dishevelled (Dvl), a central mediator of Wnt signaling, whereas its role in brea...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Yin X,Xiang T,Li L,Su X,Shu X,Luo X,Huang J,Yuan Y,Peng W,Oberst M,Kelly K,Ren G,Tao Q

    更新日期:2013-03-12 00:00:00

  • Adolescent diet and risk of breast cancer.

    abstract:BACKGROUND:Early life exposures, including diet, have been implicated in the etiology of breast cancer. METHODS:A nested case-control study was conducted among participants in the Nurses' Health Study who completed a 24-item questionnaire about diet during high school. There were 843 eligible cases diagnosed between o...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Frazier AL,Ryan CT,Rockett H,Willett WC,Colditz GA

    更新日期:2003-01-01 00:00:00

  • Estrogen and progesterone induce persistent increases in p53-dependent apoptosis and suppress mammary tumors in BALB/c-Trp53+/- mice.

    abstract:INTRODUCTION:Treatment with estrogen and progesterone (E+P) mimics the protective effect of parity on mammary tumors in rodents and depends upon the activity of p53. The following experiments tested whether exogenous E+P primes p53 to be more responsive to DNA damage and whether these pathways confer resistance to mamm...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Dunphy KA,Blackburn AC,Yan H,O'Connell LR,Jerry DJ

    更新日期:2008-01-01 00:00:00

  • Chemically induced DNA hypomethylation in breast carcinoma cells detected by the amplification of intermethylated sites.

    abstract:INTRODUCTION:Compromised patterns of gene expression result in genomic instability, altered patterns of gene expression and tumour formation. Specifically, aberrant DNA hypermethylation in gene promoter regions leads to gene silencing, whereas global hypomethylation events can result in chromosomal instability and onco...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Sadikovic B,Haines TR,Butcher DT,Rodenhiser DI

    更新日期:2004-01-01 00:00:00

  • Rack1 mediates tyrosine phosphorylation of Anxa2 by Src and promotes invasion and metastasis in drug-resistant breast cancer cells.

    abstract:BACKGROUND:Acquirement of resistance is always associated with a highly aggressive phenotype of tumor cells. Recent studies have revealed that Annexin A2 (Anxa2) is a key protein that links drug resistance and cancer metastasis. A high level of Anxa2 in cancer tissues is correlated to a highly aggressive phenotype. Inc...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Fan Y,Si W,Ji W,Wang Z,Gao Z,Tian R,Song W,Zhang H,Niu R,Zhang F

    更新日期:2019-05-22 00:00:00

  • Molecular drivers of lobular carcinoma in situ.

    abstract::Lobular carcinoma in situ (LCIS) is considered to be a risk factor for the development of invasive breast carcinoma, but it may also be a non-obligate precursor to invasive lobular carcinoma (ILC). Many LCIS lesions do not progress to ILC, and the molecular changes that are necessary for progression from LCIS to ILC a...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章,评审


    authors: Logan GJ,Dabbs DJ,Lucas PC,Jankowitz RC,Brown DD,Clark BZ,Oesterreich S,McAuliffe PF

    更新日期:2015-06-04 00:00:00

  • Rare variants in the ATM gene and risk of breast cancer.

    abstract:INTRODUCTION:The ataxia-telangiectasia mutated (ATM) gene (MIM ID 208900) encodes a protein kinase that plays a significant role in the activation of cellular responses to DNA double-strand breaks through subsequent phosphorylation of central players in the DNA damage-response pathway. Recent studies have confirmed tha...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章,多中心研究


    authors: Goldgar DE,Healey S,Dowty JG,Da Silva L,Chen X,Spurdle AB,Terry MB,Daly MJ,Buys SM,Southey MC,Andrulis I,John EM,BCFR.,kConFab.,Khanna KK,Hopper JL,Oefner PJ,Lakhani S,Chenevix-Trench G

    更新日期:2011-07-25 00:00:00

  • Correction to: Consistent and reproducible cultures of large-scale 3D mammary epithelial structures using an accessible bioprinting platform.

    abstract::Following publication of the original article [1], the authors reported a typesetting error in the spelling of the second author's name. ...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章,已发布勘误


    authors: Reid JA,Mollica PA,Bruno RD,Sachs PC

    更新日期:2018-11-19 00:00:00

  • Intra-operative spectroscopic assessment of surgical margins during breast conserving surgery.

    abstract:BACKGROUND:In over 20% of breast conserving operations, postoperative pathological assessment of the excised tissue reveals positive margins, requiring additional surgery. Current techniques for intra-operative assessment of tumor margins are insufficient in accuracy or resolution to reliably detect small tumors. There...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Shipp DW,Rakha EA,Koloydenko AA,Macmillan RD,Ellis IO,Notingher I

    更新日期:2018-07-09 00:00:00

  • From Bittner to Barr: a viral, diet and hormone breast cancer aetiology hypothesis.

    abstract::It is hypothesized that the human homologue of the mouse mammary tumour virus (HHMMTV) and other viruses, such as human papillomavirus (HPV) and Epstein-Barr virus (EBV), act as cofactors with diet, oestrogens and other hormones in the initiation and promotion of some types of breast cancer in genetically susceptible ...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章,评审


    authors: Lawson JS,Tran D,Rawlinson WD

    更新日期:2001-01-01 00:00:00

  • Receptor-interacting protein kinase 2 promotes triple-negative breast cancer cell migration and invasion via activation of nuclear factor-kappaB and c-Jun N-terminal kinase pathways.

    abstract:INTRODUCTION:Metastasis is the main cause of breast cancer morbidity and mortality. Processes that allow for tumor cell migration and invasion are important therapeutic targets. Here we demonstrate that receptor-interacting protein kinase 2 (RIP2), a kinase known to be involved in inflammatory processes, also has novel...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章


    authors: Singel SM,Batten K,Cornelius C,Jia G,Fasciani G,Barron SL,Wright WE,Shay JW

    更新日期:2014-03-19 00:00:00

  • Inheritance of deleterious mutations at both BRCA1 and BRCA2 in an international sample of 32,295 women.

    abstract:BACKGROUND:Most BRCA1 or BRCA2 mutation carriers have inherited a single (heterozygous) mutation. Transheterozygotes (TH) who have inherited deleterious mutations in both BRCA1 and BRCA2 are rare, and the consequences of transheterozygosity are poorly understood. METHODS:From 32,295 female BRCA1/2 mutation carriers, w...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章,多中心研究


    authors: Rebbeck TR,Friebel TM,Mitra N,Wan F,Chen S,Andrulis IL,Apostolou P,Arnold N,Arun BK,Barrowdale D,Benitez J,Berger R,Berthet P,Borg A,Buys SS,Caldes T,Carter J,Chiquette J,Claes KB,Couch FJ,Cybulski C,Daly MB,d

    更新日期:2016-11-11 00:00:00

  • Laminin isoform expression in breast tumors.

    abstract::Certain laminins of vascular basement membranes have been identified in human breast tumors and brain gliomas that share the same beta1 chain. These laminins are new carcinoma angiogenic markers and might represent potential targets for antiangiogenic therapy. ...

    journal_title:Breast cancer research : BCR

    pub_type: 评论,杂志文章


    authors: Holler E

    更新日期:2005-01-01 00:00:00

  • Accelerated partial breast irradiation: technically feasible but who will benefit?

    abstract::Modern breast cancer radiotherapy aims to increase uncomplicated cure rates. A priority is reduction of late effects which include chronic chest wall or breast pain, poor cosmesis, and cardiac toxicity. As breast screening detects early cancers we may be able to safely restrict irradiation postlumpectomy to the tumour...

    journal_title:Breast cancer research : BCR

    pub_type: 杂志文章,评审


    authors: Ross G

    更新日期:2005-01-01 00:00:00

  • ‘The charmingest place’: non-coding RNA, lineage tracing, tumor heterogeneity, metastasis and metabolism--new methods in mammary gland development and cancer: the fifth ENBDC Workshop.

    abstract::The European Network for Breast Development and Cancer (ENBDC) Workshop on ‘Methods in Mammary Gland Development and Cancer’ has grown into the essential, international technical discussion forum for scientists with interests in the normal and neoplastic breast. The fifth ENBDC meeting was held in Weggis, Switzerland ...

    journal_title:Breast cancer research : BCR



    authors: Clarke RB,Stingl J,Vivanco M,Bentires-Alj M

    更新日期:2013-01-01 00:00:00