Abstract:
:Spinal cord injury (SCI) is a major disability requiring more effective treatment than is currently available. MicroRNAs have been shown to effectively regulate gene expression at the translational level. The aim of the present study was to explore the potential role of miR-30-5p and possible mechanism in SCI. We found that miR-30-5p was notably down-regulated, while Neurod 1 expression was highly elevated in microglia from the mouse model of SCI. Additionally, overexpression of miR-30a-5p significantly suppressed inflammatory responses as reflected by a decrease in the secretion of the cytokines TNF-α, IL-1β and IL-10 triggered by SCI. Furthermore, introduction of miR-30a-5p strengthened the scavenging of oxygen free radicals accompanied by an increase in the expression of SEPN1, TXNL1 and GPX1. More importantly, our study explored that Neurod 1 was a direct and functional target of miR-30a-5p, which was validated by the dual luciferase reporter assay. qRT-PCR and western blot analysis further validated that miR-30a-5p negatively regulated the expression of Neurod 1. Mechanistically, overexpression of miR-30a-5p or silencing of the Neurod 1 gene prevented the MAPK/ERK signalling and inhibited inflammatory responses, meanwhile activated SEPN1, TXNL1 and GPX1. These findings indicate that miR-30a-5p ameliorates inflammatory responses and oxidative stress by targeting Neurod 1 through MAPK/ERK signalling.
journal_name
Clin Exp Pharmacol Physioljournal_title
Clinical and experimental pharmacology & physiologyauthors
Fu X,Shen Y,Wang W,Li Xdoi
10.1111/1440-1681.12856subject
Has Abstractpub_date
2018-01-01 00:00:00pages
68-74issue
1eissn
0305-1870issn
1440-1681journal_volume
45pub_type
杂志文章abstract::1. Contractions and electrical activity were recorded from isolated segments of longitudinal (L) and circular (C) myometrium from rats on gestation days 16-17 and 21 before and during parturition. 2. The segments were arranged so that the activity of the two layers could be monitored simultaneously and independently. ...
journal_title:Clinical and experimental pharmacology & physiology
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doi:10.1111/j.1440-1681.1988.tb01124.x
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journal_title:Clinical and experimental pharmacology & physiology
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doi:10.1111/j.1440-1681.1988.tb01030.x
更新日期:1988-11-01 00:00:00
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doi:10.1111/j.1440-1681.1994.tb02535.x
更新日期:1994-05-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2007-10-01 00:00:00