Derivation of therapeutic lung spheroid cells from minimally invasive transbronchial pulmonary biopsies.

Abstract:

BACKGROUND:Resident stem and progenitor cells have been identified in the lung over the last decade, but isolation and culture of these cells remains a challenge. Thus, although these lung stem and progenitor cells provide an ideal source for stem-cell based therapy, mesenchymal stem cells (MSCs) remain the most popular cell therapy product for the treatment of lung diseases. Surgical lung biopsies can be the tissue source but such procedures carry a high risk of mortality. METHODS:In this study we demonstrate that therapeutic lung cells, termed "lung spheroid cells" (LSCs) can be generated from minimally invasive transbronchial lung biopsies using a three-dimensional culture technique. The cells were then characterized by flow cytometry and immunohistochemistry. Angiogenic potential was tested by in-vitro HUVEC tube formation assay. In-vivo bio- distribution of LSCs was examined in athymic nude mice after intravenous delivery. RESULTS:From one lung biopsy, we are able to derive >50 million LSC cells at Passage 2. These cells were characterized by flow cytometry and immunohistochemistry and were shown to represent a mixture of lung stem cells and supporting cells. When introduced systemically into nude mice, LSCs were retained primarily in the lungs for up to 21 days. CONCLUSION:Here, for the first time, we demonstrated that direct culture and expansion of human lung progenitor cells from pulmonary tissues, acquired through a minimally invasive biopsy, is possible and straightforward with a three-dimensional culture technique. These cells could be utilized in long-term expansion of lung progenitor cells and as part of the development of cell-based therapies for the treatment of lung diseases such as chronic obstructive pulmonary disease (COPD) and idiopathic pulmonary fibrosis (IPF).

journal_name

Respir Res

journal_title

Respiratory research

authors

Dinh PC,Cores J,Hensley MT,Vandergriff AC,Tang J,Allen TA,Caranasos TG,Adler KB,Lobo LJ,Cheng K

doi

10.1186/s12931-017-0611-0

subject

Has Abstract

pub_date

2017-06-30 00:00:00

pages

132

issue

1

eissn

1465-9921

issn

1465-993X

pii

10.1186/s12931-017-0611-0

journal_volume

18

pub_type

杂志文章
  • Monitoring mandibular movements to detect Cheyne-Stokes Breathing.

    abstract:BACKGROUND:The patterns of mandibular movements (MM) during sleep can be used to identify increased respiratory effort periodic large-amplitude MM (LPM), and cortical arousals associated with "sharp" large-amplitude MM (SPM). We hypothesized that Cheyne Stokes breathing (CSB) may be identified by periodic abnormal MM p...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-017-0551-8

    authors: Martinot JB,Borel JC,Le-Dong NN,Guénard HJ,Cuthbert V,Silkoff PE,Gozal D,Pepin JL

    更新日期:2017-04-20 00:00:00

  • Azithromycin induces epidermal differentiation and multivesicular bodies in airway epithelia.

    abstract:BACKGROUND:Azithromycin (Azm) is a macrolide recognized for its disease-modifying effects and reduction in exacerbation of chronic airway diseases. It is not clear whether the beneficial effects of Azm are due to its anti-microbial activity or other pharmacological actions. We have shown that Azm affects the integrity ...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-019-1101-3

    authors: Arason AJ,Joelsson JP,Valdimarsdottir B,Sigurdsson S,Gudjonsson A,Halldorsson S,Johannsson F,Rolfsson O,Lehmann F,Ingthorsson S,Cherek P,Gudmundsson GH,Gardarsson FR,Page CP,Baldursson O,Gudjonsson T,Kricker JA

    更新日期:2019-06-24 00:00:00

  • ADAM15 expression is increased in lung CD8+ T cells, macrophages, and bronchial epithelial cells in patients with COPD and is inversely related to airflow obstruction.

    abstract:BACKGROUND:A disintegrin and metalloproteinase domain-15 (ADAM15) is expressed by activated leukocytes, and fibroblasts in vitro. Whether ADAM15 expression is increased in the lungs of COPD patients is not known. METHODS:ADAM15 gene expression and/or protein levels were measured in whole lung and bronchoalveolar lavag...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-020-01446-5

    authors: Wang X,Zhang D,Higham A,Wolosianka S,Gai X,Zhou L,Petersen H,Pinto-Plata V,Divo M,Silverman EK,Celli B,Singh D,Sun Y,Owen CA

    更新日期:2020-07-16 00:00:00

  • Auxiliary diagnostic value of tumor biomarkers in pleural fluid for lung cancer-associated malignant pleural effusion.

    abstract:BACKGROUND:Pleural effusion (PE) can be divided into benign pleural effusion (BPE) and malignant pleural effusion (MPE). There is no consensus on the identification of lung cancer-associated MPE using the optimal cut-off levels from five common tumor biomarkers (CEA, CYFRA 21-1, CA125, SCC-Ag, and NSE). Therefore, we a...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-020-01557-z

    authors: Zhang H,Li C,Hu F,Zhang X,Shen Y,Chen Y,Li F

    更新日期:2020-10-29 00:00:00

  • Impaired anti-inflammatory action of glucocorticoid in neutrophil from patients with steroid-resistant asthma.

    abstract:BACKGROUND:Steroid resistant (SR) asthma is characterized by persistent airway inflammation that fails to resolve despite treatment with high doses of corticosteroids. Furthermore, SR patient airways show increased numbers neutrophils, which are less responsive to glucocorticoid. The present study seeks to determine wh...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-016-0462-0

    authors: Wang M,Gao P,Wu X,Chen Y,Feng Y,Yang Q,Xu Y,Zhao J,Xie J

    更新日期:2016-11-16 00:00:00

  • Oral tolerance inhibits pulmonary eosinophilia in a cockroach allergen induced model of asthma: a randomized laboratory study.

    abstract:BACKGROUND:Antigen desensitization through oral tolerance is becoming an increasingly attractive treatment option for allergic diseases. However, the mechanism(s) by which tolerization is achieved remain poorly defined. In this study we endeavored to induce oral tolerance to cockroach allergen (CRA: a complex mixture o...

    journal_title:Respiratory research

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/1465-9921-11-160

    authors: Vaickus LJ,Bouchard J,Kim J,Natarajan S,Remick DG

    更新日期:2010-11-23 00:00:00

  • A retinoid X receptor partial agonist attenuates pulmonary emphysema and airway inflammation.

    abstract:BACKGROUND:Retinoid X receptors (RXRs) are members of the nuclear receptor (NR) superfamily that mediate signalling by 9-cis retinoic acid, a vitamin A derivative. RXRs play key roles not only as homodimers but also as heterodimeric partners, e.g., for retinoic acid receptors, vitamin D receptors, and peroxisome prolif...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-018-0963-0

    authors: Morichika D,Miyahara N,Fujii U,Taniguchi A,Oda N,Senoo S,Kataoka M,Tanimoto M,Kakuta H,Kiura K,Maeda Y,Kanehiro A

    更新日期:2019-01-03 00:00:00

  • Relationship between age and bronchodilator response at diagnosis in adult-onset asthma.

    abstract:BACKGROUND:Possible variation in bronchodilator response (BDR) according to age at the diagnosis of adult-onset asthma is unknown. Our aim was to assess if BDR in FEV1 is related to age at diagnosis of adult-onset asthma and how many subjects fulfill the 400 mL criterion of BDR, the suggested cut-off for asthma-like re...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-020-01441-w

    authors: Tommola M,Won HK,Ilmarinen P,Jung H,Tuomisto LE,Lehtimäki L,Niemelä O,Kim TB,Kankaanranta H

    更新日期:2020-07-13 00:00:00

  • Differences in the risk of mood disorders in patients with asthma-COPD overlap and in patients with COPD alone: a nationwide population-based retrospective cohort study in Korea.

    abstract:BACKGROUND:Although feelings of anxiety and depression are common in patients with chronic obstructive pulmonary disease (COPD), little is known about the estimates of their incidence in patients with asthma-COPD overlap (ACO), which has been described and acknowledged as a distinct clinical entity. We aimed to estimat...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-019-1039-5

    authors: Kang HR,Hong SH,Ha SY,Kim TB,Lee EK

    更新日期:2019-04-30 00:00:00

  • Thank you and farewell after 15 years editing respiratory research.

    abstract:: ...

    journal_title:Respiratory research

    pub_type: 社论

    doi:10.1186/s12931-018-0929-2

    authors: Lotvall J,Panettieri RA Jr

    更新日期:2018-11-26 00:00:00

  • Nebulization of the acidified sodium nitrite formulation attenuates acute hypoxic pulmonary vasoconstriction.

    abstract:BACKGROUND:Generalized hypoxic pulmonary vasoconstriction (HPV) occurring during exposure to hypoxia is a detrimental process resulting in an increase in lung vascular resistance. Nebulization of sodium nitrite has been shown to inhibit HPV. The aim of this project was to investigate and compare the effects of nebuliza...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-11-81

    authors: Egemnazarov B,Schermuly RT,Dahal BK,Elliott GT,Hoglen NC,Surber MW,Weissmann N,Grimminger F,Seeger W,Ghofrani HA

    更新日期:2010-06-21 00:00:00

  • The need for fast-track, high-quality and low-cost studies about the role of the BCG vaccine in the fight against COVID-19.

    abstract::Bacillus Calmette-Guérin (BCG) vaccination is routine and near-universal in many low- and middle-income countries (LMIC). It has been suggested that BCG can have a protective effect on COVID-19 morbidity and mortality. This commentary discusses the limitations of the evidence around BCG and COVID-19. We argue that hig...

    journal_title:Respiratory research

    pub_type: 信件,评审

    doi:10.1186/s12931-020-01439-4

    authors: Pereira M,Paixão E,Trajman A,de Souza RA,da Natividade MS,Pescarini JM,Pereira SM,Barreto FR,Ximenes R,Dalcomo M,Ichihara MY,Nunes C,Barral-Netto M,Barreto ML

    更新日期:2020-07-11 00:00:00

  • Surfactant protein D attenuates sub-epithelial fibrosis in allergic airways disease through TGF-β.

    abstract:BACKGROUND:Surfactant protein D (SP-D) can regulate both innate and adaptive immunity. Recently, SP-D has been shown to contribute to the pathogenesis of airway allergic inflammation and bleomycin-induced pulmonary fibrosis. However, in allergic airways disease, the role of SP-D in airway remodeling remains unknown. Th...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-014-0143-9

    authors: Ogawa H,Ledford JG,Mukherjee S,Aono Y,Nishioka Y,Lee JJ,Izumi K,Hollingsworth JW

    更新日期:2014-11-29 00:00:00

  • Successful establishment of primary small airway cell cultures in human lung transplantation.

    abstract:BACKGROUND:The study of small airway diseases such as post-transplant bronchiolitis obliterans syndrome (BOS) is hampered by the difficulty in assessing peripheral airway function either physiologically or directly. Our aims were to develop robust methods for sampling small airway epithelial cells (SAEC) and to establi...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-10-99

    authors: Banerjee B,Kicic A,Musk M,Sutanto EN,Stick SM,Chambers DC

    更新日期:2009-10-26 00:00:00

  • Analysis of comorbid factors that increase the COPD assessment test scores.

    abstract:BACKGROUND:The chronic obstructive pulmonary disease (COPD) Assessment Test (CAT) is a concise health status measure for COPD. COPD patients have a variety of comorbidities, but little is known about their impact on quality of life. This study was designed to investigate comorbid factors that may contribute to high CAT...

    journal_title:Respiratory research

    pub_type: 临床试验,杂志文章

    doi:10.1186/1465-9921-15-13

    authors: Miyazaki M,Nakamura H,Chubachi S,Sasaki M,Haraguchi M,Yoshida S,Tsuduki K,Shirahata T,Takahashi S,Minematsu N,Koh H,Nakamura M,Sakamaki F,Terashima T,Sayama K,Jones PW,Asano K,Betsuyaku T,Keio COPD Comorbidity Researc

    更新日期:2014-02-06 00:00:00

  • Allergen particle binding by human primary bronchial epithelial cells is modulated by surfactant protein D.

    abstract:BACKGROUND:Allergen-containing subpollen particles (SPP) are released from whole plant pollen upon contact with water or even high humidity. Because of their size SPP can preferentially reach the lower airways where they come into contact with surfactant protein (SP)-D. Our previous work demonstrated that SP-D increase...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-11-83

    authors: Schleh C,Erpenbeck VJ,Winkler C,Lauenstein HD,Nassimi M,Braun A,Krug N,Hohlfeld JM

    更新日期:2010-06-22 00:00:00

  • Factors affecting exhaled nitric oxide measurements: the effect of sex.

    abstract:BACKGROUND:Exhaled nitric oxide (F(E)NO) measurements are used as a surrogate marker for eosinophilic airway inflammation. However, many constitutional and environmental factors affect F(E)NO, making it difficult to devise reference values. Our aim was to evaluate the relative importance of factors affecting F(E)NO in ...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-8-82

    authors: Taylor DR,Mandhane P,Greene JM,Hancox RJ,Filsell S,McLachlan CR,Williamson AJ,Cowan JO,Smith AD,Sears MR

    更新日期:2007-11-15 00:00:00

  • Epigenetics dysfunction in morbid obesity with or without obstructive sleep apnoea: the EPIMOOSA study.

    abstract:BACKGROUND:Obstructive sleep apnoea (OSA) and morbid obesity (MO), defined by a body mass index ≥35 kg/m2, are two closely related conditions. Recent studies suggest that circulating microRNA (miRNA) plays a potential role in the physiopathology of both conditions. To date, circulating miRNA expression has been studied...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-020-1302-9

    authors: Lázaro J,Clavería P,Cabrejas C,Fernando J,Daga B,Ordoñez B,Segura S,Sanz-Rubio D,Marín JM

    更新日期:2020-02-04 00:00:00

  • BMI is associated with FEV1 decline in chronic obstructive pulmonary disease: a meta-analysis of clinical trials.

    abstract:BACKGROUND:There is considerable heterogeneity in the rate of lung function decline in chronic obstructive pulmonary disease (COPD), the determinants of which are largely unknown. Observational studies in COPD indicate that low body mass index (BMI) is associated with worse outcomes, and overweight/obesity has a protec...

    journal_title:Respiratory research

    pub_type: 杂志文章,meta分析

    doi:10.1186/s12931-019-1209-5

    authors: Sun Y,Milne S,Jaw JE,Yang CX,Xu F,Li X,Obeidat M,Sin DD

    更新日期:2019-10-29 00:00:00

  • Pectoralis muscle area and mortality in smokers without airflow obstruction.

    abstract:BACKGROUND:Low muscle mass is associated with increased mortality in the general population but its prognostic value in at-risk smokers, those without expiratory airflow obstruction, is unknown. We aimed to test the hypothesis that reduced muscle mass is associated with increased mortality in at-risk smokers. METHODS:...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-018-0771-6

    authors: Diaz AA,Martinez CH,Harmouche R,Young TP,McDonald ML,Ross JC,Han ML,Bowler R,Make B,Regan EA,Silverman EK,Crapo J,Boriek AM,Kinney GL,Hokanson JE,Estepar RSJ,Washko GR

    更新日期:2018-04-10 00:00:00

  • The cellular kinetics of lung alveolar epithelial cells and its relationship with lung tissue repair after acute lung injury.

    abstract:BACKGROUND:Organ regeneration in mammals is hypothesized to require a functional pool of stem or progenitor cells, but the role of these cells in lung regeneration is unknown. METHODS:Based on the fact that postnatal regeneration of alveolar tissue has been attributed to alveolar epithelial cells, we established a hem...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-016-0480-y

    authors: Zeng L,Yang XT,Li HS,Li Y,Yang C,Gu W,Zhou YH,Du J,Wang HY,Sun JH,Wen DL,Jiang JX

    更新日期:2016-12-07 00:00:00

  • Bidirectional role of IL-6 signal in pathogenesis of lung fibrosis.

    abstract:BACKGROUND:Various signals are known to participate in the pathogenesis of lung fibrosis. Our aim was to determine which signal is predominantly mobilized in the early inflammatory phase and thereafter modulates the development of lung fibrosis. METHODS:Mice received a single dose of 3 mg/kg body weight of bleomycin (...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-015-0261-z

    authors: Kobayashi T,Tanaka K,Fujita T,Umezawa H,Amano H,Yoshioka K,Naito Y,Hatano M,Kimura S,Tatsumi K,Kasuya Y

    更新日期:2015-08-20 00:00:00

  • Primary data, claims data, and linked data in observational research: the case of COPD in Germany.

    abstract:BACKGROUND:Real-world evidence (RWE) can inform patient management decisions, but RWE studies are associated with limitations. Linkage of different RWE data types could address such limitations by enriching data and improving scientific quality. Using the example of chronic obstructive pulmonary disease (COPD) in Germa...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-018-0865-1

    authors: Mueller S,Gottschalk F,Groth A,Meeraus W,Driessen M,Kohlmann T,Wilke T

    更新日期:2018-08-30 00:00:00

  • Chronic exposure of diesel exhaust particles induces alveolar enlargement in mice.

    abstract:BACKGROUND:Diesel exhaust particles (DEPs) are deposited into the respiratory tract and are thought to be a risk factor for the development of diseases of the respiratory system. In healthy individuals, the timing and mechanisms of respiratory tract injuries caused by chronic exposure to air pollution remain to be clar...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-015-0172-z

    authors: Yoshizaki K,Brito JM,Moriya HT,Toledo AC,Ferzilan S,Ligeiro de Oliveira AP,Machado ID,Farsky SH,Silva LF,Martins MA,Saldiva PH,Mauad T,Macchione M

    更新日期:2015-02-07 00:00:00

  • Activation of the nicotinamide N-methyltransferase (NNMT)-1-methylnicotinamide (MNA) pathway in pulmonary hypertension.

    abstract:BACKGROUND:Pulmonary arterial hypertension (PAH) is associated with inflammatory response but it is unknown whether it is associated with alterations in NNMT activity and MNA plasma concentration. Here we examined changes in NNMT-MNA pathway in PAH in rats and humans. METHODS:PAH in rats was induced by a single subcut...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-016-0423-7

    authors: Fedorowicz A,Mateuszuk Ł,Kopec G,Skórka T,Kutryb-Zając B,Zakrzewska A,Walczak M,Jakubowski A,Łomnicka M,Słomińska E,Chlopicki S

    更新日期:2016-08-31 00:00:00

  • Macrorheology of cystic fibrosis, chronic obstructive pulmonary disease & normal sputum.

    abstract:BACKGROUND:Prior microrheologic assessments of selected, microlitre plugs of cystic fibrosis (CF) sputum suggest no intrinsic rheologic abnormality. However, such analyses may not be representative of CF sputum as a whole. We therefore reassessed this question using whole sputum macrorheology. Additionally, we wished t...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-10-63

    authors: Serisier DJ,Carroll MP,Shute JK,Young SA

    更新日期:2009-07-06 00:00:00

  • Local therapy with CpG motifs in a murine model of allergic airway inflammation in IFN-beta knock-out mice.

    abstract:BACKGROUND:CpG oligodeoxynucleotides (CpG-ODN) are capable of inducing high amounts of type I IFNs with many immunomodulatory properties. Furthermore, type-I IFNs have been proposed to play a key role in mediating effects of CpG-ODN. The precise role of IFN-beta in the immunomodulatory effects of CpG-ODN is not known. ...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-6-25

    authors: Matheu V,Treschow A,Teige I,Navikas V,Issazadeh-Navikas S

    更新日期:2005-03-05 00:00:00

  • Indacaterol and glycopyrronium versus indacaterol on body plethysmography measurements in COPD-a randomised controlled study.

    abstract:BACKGROUND:Dual bronchodilator therapy is recommended for symptomatic patients with chronic obstructive pulmonary disease (COPD). There are limited data on effects of a combination of two long-acting bronchodilators on lung function including body plethysmography. METHODS:This multicentre, randomised, double-blind, si...

    journal_title:Respiratory research

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1186/s12931-016-0498-1

    authors: Salomon J,Stolz D,Domenighetti G,Frey JG,Turk AJ,Azzola A,Sigrist T,Fitting JW,Schmidt U,Geiser T,Wild C,Kostikas K,Clemens A,Brutsche M

    更新日期:2017-01-11 00:00:00

  • Fibrocytes are increased in lung and peripheral blood of patients with idiopathic pulmonary fibrosis.

    abstract:BACKGROUND:Fibrocytes are implicated in Idiopathic Pulmonary Fibrosis (IPF) pathogenesis and increased proportions in the circulation are associated with poor prognosis. Upon tissue injury, fibrocytes migrate to the affected organ. In IPF patients, circulating fibrocytes are increased especially during exacerbations, h...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-018-0798-8

    authors: Heukels P,van Hulst JAC,van Nimwegen M,Boorsma CE,Melgert BN,van den Toorn LM,Boomars KAT,Wijsenbeek MS,Hoogsteden H,von der Thüsen JH,Hendriks RW,Kool M,van den Blink B

    更新日期:2018-05-10 00:00:00

  • The clinical course of idiopathic pulmonary fibrosis and its association to quality of life over time: longitudinal data from the INSIGHTS-IPF registry.

    abstract:BACKGROUND:Quality of life (QoL) is profoundly impaired in patients with idiopathic pulmonary fibrosis (IPF). However, data is limited regarding the course of QoL. We therefore analysed longitudinal data from the German INSIGHTS-IPF registry. METHODS:Clinical status and QoL were assessed at enrollment and subsequently...

    journal_title:Respiratory research

    pub_type: 杂志文章,多中心研究

    doi:10.1186/s12931-019-1020-3

    authors: Kreuter M,Swigris J,Pittrow D,Geier S,Klotsche J,Prasse A,Wirtz H,Koschel D,Andreas S,Claussen M,Grohé C,Wilkens H,Hagmeyer L,Skowasch D,Meyer JF,Kirschner J,Gläser S,Kahn N,Welte T,Neurohr C,Schwaiblmair M,Held

    更新日期:2019-03-15 00:00:00