Abstract:
BACKGROUND:Resident stem and progenitor cells have been identified in the lung over the last decade, but isolation and culture of these cells remains a challenge. Thus, although these lung stem and progenitor cells provide an ideal source for stem-cell based therapy, mesenchymal stem cells (MSCs) remain the most popular cell therapy product for the treatment of lung diseases. Surgical lung biopsies can be the tissue source but such procedures carry a high risk of mortality. METHODS:In this study we demonstrate that therapeutic lung cells, termed "lung spheroid cells" (LSCs) can be generated from minimally invasive transbronchial lung biopsies using a three-dimensional culture technique. The cells were then characterized by flow cytometry and immunohistochemistry. Angiogenic potential was tested by in-vitro HUVEC tube formation assay. In-vivo bio- distribution of LSCs was examined in athymic nude mice after intravenous delivery. RESULTS:From one lung biopsy, we are able to derive >50 million LSC cells at Passage 2. These cells were characterized by flow cytometry and immunohistochemistry and were shown to represent a mixture of lung stem cells and supporting cells. When introduced systemically into nude mice, LSCs were retained primarily in the lungs for up to 21 days. CONCLUSION:Here, for the first time, we demonstrated that direct culture and expansion of human lung progenitor cells from pulmonary tissues, acquired through a minimally invasive biopsy, is possible and straightforward with a three-dimensional culture technique. These cells could be utilized in long-term expansion of lung progenitor cells and as part of the development of cell-based therapies for the treatment of lung diseases such as chronic obstructive pulmonary disease (COPD) and idiopathic pulmonary fibrosis (IPF).
journal_name
Respir Resjournal_title
Respiratory researchauthors
Dinh PC,Cores J,Hensley MT,Vandergriff AC,Tang J,Allen TA,Caranasos TG,Adler KB,Lobo LJ,Cheng Kdoi
10.1186/s12931-017-0611-0subject
Has Abstractpub_date
2017-06-30 00:00:00pages
132issue
1eissn
1465-9921issn
1465-993Xpii
10.1186/s12931-017-0611-0journal_volume
18pub_type
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journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-017-0551-8
更新日期:2017-04-20 00:00:00
abstract:BACKGROUND:Azithromycin (Azm) is a macrolide recognized for its disease-modifying effects and reduction in exacerbation of chronic airway diseases. It is not clear whether the beneficial effects of Azm are due to its anti-microbial activity or other pharmacological actions. We have shown that Azm affects the integrity ...
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pub_type: 杂志文章
doi:10.1186/s12931-019-1101-3
更新日期:2019-06-24 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12931-020-01446-5
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journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-020-01557-z
更新日期:2020-10-29 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-016-0462-0
更新日期:2016-11-16 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章,随机对照试验
doi:10.1186/1465-9921-11-160
更新日期:2010-11-23 00:00:00
abstract:BACKGROUND:Retinoid X receptors (RXRs) are members of the nuclear receptor (NR) superfamily that mediate signalling by 9-cis retinoic acid, a vitamin A derivative. RXRs play key roles not only as homodimers but also as heterodimeric partners, e.g., for retinoic acid receptors, vitamin D receptors, and peroxisome prolif...
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pub_type: 杂志文章
doi:10.1186/s12931-018-0963-0
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journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-020-01441-w
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journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-019-1039-5
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abstract:: ...
journal_title:Respiratory research
pub_type: 社论
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journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-11-81
更新日期:2010-06-21 00:00:00
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pub_type: 信件,评审
doi:10.1186/s12931-020-01439-4
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journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-014-0143-9
更新日期:2014-11-29 00:00:00
abstract:BACKGROUND:The study of small airway diseases such as post-transplant bronchiolitis obliterans syndrome (BOS) is hampered by the difficulty in assessing peripheral airway function either physiologically or directly. Our aims were to develop robust methods for sampling small airway epithelial cells (SAEC) and to establi...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-10-99
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abstract:BACKGROUND:The chronic obstructive pulmonary disease (COPD) Assessment Test (CAT) is a concise health status measure for COPD. COPD patients have a variety of comorbidities, but little is known about their impact on quality of life. This study was designed to investigate comorbid factors that may contribute to high CAT...
journal_title:Respiratory research
pub_type: 临床试验,杂志文章
doi:10.1186/1465-9921-15-13
更新日期:2014-02-06 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-11-83
更新日期:2010-06-22 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-8-82
更新日期:2007-11-15 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12931-020-1302-9
更新日期:2020-02-04 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章,meta分析
doi:10.1186/s12931-019-1209-5
更新日期:2019-10-29 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12931-018-0771-6
更新日期:2018-04-10 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12931-016-0480-y
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journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-015-0261-z
更新日期:2015-08-20 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12931-018-0865-1
更新日期:2018-08-30 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-015-0172-z
更新日期:2015-02-07 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-016-0423-7
更新日期:2016-08-31 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-10-63
更新日期:2009-07-06 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-6-25
更新日期:2005-03-05 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/s12931-016-0498-1
更新日期:2017-01-11 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-018-0798-8
更新日期:2018-05-10 00:00:00
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journal_title:Respiratory research
pub_type: 杂志文章,多中心研究
doi:10.1186/s12931-019-1020-3
更新日期:2019-03-15 00:00:00