Genome-wide DNA methylation analysis in lung fibroblasts co-cultured with silica-exposed alveolar macrophages.

Abstract:

BACKGROUND:Exposure to crystalline silica is considered to increase the risk of lung fibrosis. The primary effector cell, the myofibroblast, plays an important role in the deposition of extracellular matrix (ECM). DNA methylation change is considered to have a potential effect on myofibroblast differentiation. Therefore, the present study was designed to investigate the genome-wide DNA methylation profiles of lung fibroblasts co-cultured with alveolar macrophages exposed to crystalline silica in vitro. METHODS:AM/fibroblast co-culture system was established. CCK8 was used to assess the toxicity of AMs. mRNA and protein expression of collagen I, α-SMA, MAPK9 and TGF-β1 of fibroblasts after AMs exposed to 100 μg /ml SiO2 for 0-, 24-, or 48 h were determined by means of quantitative real-time PCR, immunoblotting and immunohistochemistry. Genomic DNA of fibroblasts was isolated using MeDIP-Seq to sequence. R software, GO, KEGG and Cytoscape were used to analyze the data. RESULTS:SiO2 exposure increased the expression of collagen I and α-SMA in fibroblasts in co-culture system. Analysis of fibroblast methylome identified extensive methylation changes involved in several signaling pathways, such as the MAPK signaling pathway and metabolic pathways. Several candidates, including Tgfb1 and Mapk9, are hubs who can connect the gene clusters. MAPK9 mRNA expression was significantly higher in fibroblast exposed to SiO2 in co-culture system for 48 h. MAPK9 protein expression was increased at both 24-h and 48-h treatment groups. TGF-β1 mRNA expression of fibroblast has a time-dependent manner, but we didn't observe the TGF-β1 protein expression. CONCLUSION:Tgfb1 and Mapk9 are helpful to explore the mechanism of myofibroblast differentiation. The genome-wide DNA methylation profiles of fibroblasts in this experimental silicosis model will be useful for future studies on epigenetic gene regulation during myofibroblast differentiation.

journal_name

Respir Res

journal_title

Respiratory research

authors

Li J,Yao W,Zhang L,Bao L,Chen H,Wang D,Yue Z,Li Y,Zhang M,Hao C

doi

10.1186/s12931-017-0576-z

subject

Has Abstract

pub_date

2017-05-12 00:00:00

pages

91

issue

1

eissn

1465-9921

issn

1465-993X

pii

10.1186/s12931-017-0576-z

journal_volume

18

pub_type

杂志文章
  • A candidate gene identification strategy utilizing mouse to human big-data mining: "3R-tenet" in COPD genetic research.

    abstract:BACKGROUND:Early life impairments leading to lower lung function by adulthood are considered as risk factors for chronic obstructive pulmonary disease (COPD). Recently, we compared the lung transcriptomic profile between two mouse strains with extreme total lung capacities to identify plausible pulmonary function deter...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-018-0795-y

    authors: Vishweswaraiah S,George L,Purushothaman N,Ganguly K

    更新日期:2018-06-06 00:00:00

  • Medroxyprogesterone improves nocturnal breathing in postmenopausal women with chronic obstructive pulmonary disease.

    abstract:BACKGROUND:Progestins as respiratory stimulants in chronic obstructive pulmonary disease (COPD) have been investigated in males and during wakefulness. However, sleep and gender may influence therapeutic responses. We investigated the effects of a 2-week medroxyprogesterone acetate (MPA) therapy on sleep and nocturnal ...

    journal_title:Respiratory research

    pub_type: 临床试验,杂志文章

    doi:10.1186/1465-9921-6-28

    authors: Saaresranta T,Aittokallio T,Utriainen K,Polo O

    更新日期:2005-04-04 00:00:00

  • Role of ATP-sensitive potassium channels on hypoxic pulmonary vasoconstriction in endotoxemia.

    abstract:BACKGROUND:ATP-regulated potassium channels (KATP) regulate pulmonary vascular tone and are involved in hypoxic pulmonary vasoconstriction (HPV). In patients with inflammation like sepsis or ARDS, HPV is impaired, resulting in a ventilation-perfusion mismatch and hypoxia. Since increase of vascular KATP channel Kir6.1 ...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-018-0735-x

    authors: Turzo M,Vaith J,Lasitschka F,Weigand MA,Busch CJ

    更新日期:2018-02-13 00:00:00

  • Lung function reductions associated with motor vehicle density in chronic obstructive pulmonary disease: a cross-sectional study.

    abstract:BACKGROUND:Motor vehicle-related air pollution can potentially impair lung function. The effect of pollution in people with compromised pulmonary function such as in COPD has not been previously investigated. To examine the association of lung function with motor vehicle density in people with spirometrically determine...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-016-0451-3

    authors: Nitschke M,Appleton SL,Li Q,Tucker GR,Shah P,Bi P,Pisaniello DL,Adams RJ

    更新日期:2016-10-24 00:00:00

  • Rituximab therapy in pulmonary alveolar proteinosis improves alveolar macrophage lipid homeostasis.

    abstract:RATIONALE:Pulmonary Alveolar Proteinosis (PAP) patients exhibit an acquired deficiency of biologically active granulocyte-macrophage colony stimulating factor (GM-CSF) attributable to GM-CSF specific autoantibodies. PAP alveolar macrophages are foamy, lipid-filled cells with impaired surfactant clearance and markedly r...

    journal_title:Respiratory research

    pub_type: 临床试验,杂志文章

    doi:10.1186/1465-9921-13-46

    authors: Malur A,Kavuru MS,Marshall I,Barna BP,Huizar I,Karnekar R,Thomassen MJ

    更新日期:2012-06-14 00:00:00

  • Pharmacological inhibition of GSK-3 in a guinea pig model of LPS-induced pulmonary inflammation: II. Effects on skeletal muscle atrophy.

    abstract:BACKGROUND:Chronic obstructive pulmonary disease (COPD) is accompanied by pulmonary inflammation and associated with extra-pulmonary manifestations, including skeletal muscle atrophy. Glycogen synthase kinase-3 (GSK-3) has been implicated in the regulation of muscle protein- and myonuclear turnover; two crucial process...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-14-117

    authors: Verhees KJ,Pansters NA,Baarsma HA,Remels AH,Haegens A,de Theije CC,Schols AM,Gosens R,Langen RC

    更新日期:2013-11-01 00:00:00

  • CCR2 and CXCR3 agonistic chemokines are differently expressed and regulated in human alveolar epithelial cells type II.

    abstract::The attraction of leukocytes from circulation to inflamed lungs depends on the activation of both the leukocytes and the resident cells within the lung. In this study we determined gene expression and secretion patterns for monocyte chemoattractant protein-1 (MCP-1/CCL2) and T-cell specific CXCR3 agonistic chemokines ...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-6-75

    authors: Pechkovsky DV,Goldmann T,Ludwig C,Prasse A,Vollmer E,Müller-Quernheim J,Zissel G

    更新日期:2005-07-20 00:00:00

  • Real world evaluation of a novel lateral flow assay (AlphaKit® QuickScreen) for the detection of alpha-1-antitrypsin deficiency.

    abstract:BACKGROUND:Alpha-1-Antitrypsin (AAT) deficiency (AATD) is a hereditary disorder that manifests primarily as pulmonary emphysema and liver cirrhosis. The clinically most relevant mutation causing AATD is a single nucleotide polymorphism Glu342Lys (Z-mutation). Despite the recommendation to test every COPD patient, the c...

    journal_title:Respiratory research

    pub_type: 杂志文章,多中心研究

    doi:10.1186/s12931-018-0826-8

    authors: Greulich T,Rodríguez-Frias F,Belmonte I,Klemmer A,Vogelmeier CF,Miravitlles M

    更新日期:2018-08-13 00:00:00

  • Therapeutic effect of budesonide/formoterol, montelukast and N-acetylcysteine for bronchiolitis obliterans syndrome after hematopoietic stem cell transplantation.

    abstract:BACKGROUND:Bronchiolitis obliterans syndrome (BOS) after allogeneic hematopoietic stem cell transplantation (HSCT) is currently treated with systemic corticosteroids despite poor efficacy and side effects. This study investigated the therapeutic effect of budesonide/formoterol, montelukast and n-acetylcysteine, which a...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-016-0380-1

    authors: Kim SW,Rhee CK,Kim YJ,Lee S,Kim HJ,Lee JW

    更新日期:2016-05-26 00:00:00

  • Blood and sputum eosinophils in COPD; relationship with bacterial load.

    abstract:BACKGROUND:Sputum and blood eosinophil counts predict corticosteroid effects in COPD patients. Bacterial infection causes increased airway neutrophilic inflammation. The relationship of eosinophil counts with airway bacterial load in COPD patients is uncertain. We tested the hypothesis that bacterial load and eosinophi...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-017-0570-5

    authors: Kolsum U,Donaldson GC,Singh R,Barker BL,Gupta V,George L,Webb AJ,Thurston S,Brookes AJ,McHugh TD,Wedzicha JA,Brightling CE,Singh D

    更新日期:2017-05-08 00:00:00

  • Pectoralis muscle area and mortality in smokers without airflow obstruction.

    abstract:BACKGROUND:Low muscle mass is associated with increased mortality in the general population but its prognostic value in at-risk smokers, those without expiratory airflow obstruction, is unknown. We aimed to test the hypothesis that reduced muscle mass is associated with increased mortality in at-risk smokers. METHODS:...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-018-0771-6

    authors: Diaz AA,Martinez CH,Harmouche R,Young TP,McDonald ML,Ross JC,Han ML,Bowler R,Make B,Regan EA,Silverman EK,Crapo J,Boriek AM,Kinney GL,Hokanson JE,Estepar RSJ,Washko GR

    更新日期:2018-04-10 00:00:00

  • Artificial intelligence accuracy in detecting pathological breath sounds in children using digital stethoscopes.

    abstract:BACKGROUND:Manual auscultation to detect abnormal breath sounds has poor inter-observer reliability. Digital stethoscopes with artificial intelligence (AI) could improve reliable detection of these sounds. We aimed to independently test the abilities of AI developed for this purpose. METHODS:One hundred and ninety two...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-020-01523-9

    authors: Kevat A,Kalirajah A,Roseby R

    更新日期:2020-09-29 00:00:00

  • Tissue fibrocytes in patients with mild asthma: a possible link to thickness of reticular basement membrane?

    abstract:BACKGROUND:Myofibroblasts, proposed as being derived from circulating fibrocytes, are considered to be important cells in thickening of the basement membrane in patients with asthma. We have studied the correlation of tissue fibrocyte levels to basement membrane thickness and the presence of fibrocytes in bronchoalveol...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-7-50

    authors: Nihlberg K,Larsen K,Hultgårdh-Nilsson A,Malmström A,Bjermer L,Westergren-Thorsson G

    更新日期:2006-03-29 00:00:00

  • BMI is associated with FEV1 decline in chronic obstructive pulmonary disease: a meta-analysis of clinical trials.

    abstract:BACKGROUND:There is considerable heterogeneity in the rate of lung function decline in chronic obstructive pulmonary disease (COPD), the determinants of which are largely unknown. Observational studies in COPD indicate that low body mass index (BMI) is associated with worse outcomes, and overweight/obesity has a protec...

    journal_title:Respiratory research

    pub_type: 杂志文章,meta分析

    doi:10.1186/s12931-019-1209-5

    authors: Sun Y,Milne S,Jaw JE,Yang CX,Xu F,Li X,Obeidat M,Sin DD

    更新日期:2019-10-29 00:00:00

  • Estimating the U.S. prevalence of chronic obstructive pulmonary disease using pre- and post-bronchodilator spirometry: the National Health and Nutrition Examination Survey (NHANES) 2007-2010.

    abstract:BACKGROUND:During 2007-2010, the National Health and Nutrition Examination Survey (NHANES) conducted a spirometry component which obtained pre-bronchodilator pulmonary lung function data on a nationally representative sample of US adults aged 6-79 years and post-bronchodilator pulmonary lung function data for the subse...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-14-103

    authors: Tilert T,Dillon C,Paulose-Ram R,Hnizdo E,Doney B

    更新日期:2013-10-09 00:00:00

  • Childhood infections and asthma: at the crossroads of the hygiene and Barker hypotheses.

    abstract::The hygiene hypothesis states that childhood asthma develops as a result of decreased exposure to infectious agents during infancy and early childhood. This results in the persistence of the neonatal T helper lymphocyte 2 immunophenotype, thereby predisposing the child to atopic disease. While multiple studies support...

    journal_title:Respiratory research

    pub_type: 杂志文章,评审

    doi:10.1186/rr81

    authors: Tantisira KG,Weiss ST

    更新日期:2001-01-01 00:00:00

  • Microbial dysbiosis and mortality during mechanical ventilation: a prospective observational study.

    abstract:BACKGROUND:Host-associated microbial communities have important roles in tissue homeostasis and overall health. Severe perturbations can occur within these microbial communities during critical illness due to underlying diseases and clinical interventions, potentially influencing patient outcomes. We sought to profile ...

    journal_title:Respiratory research

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/s12931-018-0950-5

    authors: Lamarche D,Johnstone J,Zytaruk N,Clarke F,Hand L,Loukov D,Szamosi JC,Rossi L,Schenck LP,Verschoor CP,McDonald E,Meade MO,Marshall JC,Bowdish DME,Karachi T,Heels-Ansdell D,Cook DJ,Surette MG,PROSPECT Investigators.,C

    更新日期:2018-12-07 00:00:00

  • Surfactant protein D attenuates sub-epithelial fibrosis in allergic airways disease through TGF-β.

    abstract:BACKGROUND:Surfactant protein D (SP-D) can regulate both innate and adaptive immunity. Recently, SP-D has been shown to contribute to the pathogenesis of airway allergic inflammation and bleomycin-induced pulmonary fibrosis. However, in allergic airways disease, the role of SP-D in airway remodeling remains unknown. Th...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-014-0143-9

    authors: Ogawa H,Ledford JG,Mukherjee S,Aono Y,Nishioka Y,Lee JJ,Izumi K,Hollingsworth JW

    更新日期:2014-11-29 00:00:00

  • Factors associated with hospitalization in bronchiectasis exacerbations: a one-year follow-up study.

    abstract:BACKGROUND:Bronchiectasis (BE) is a chronic structural lung disease with frequent exacerbations, some of which require hospital admission though no clear associated factors have been identified. We aimed to evaluate factors associated with hospitalization due to exacerbations during a 1-year follow-up period. METHODS:...

    journal_title:Respiratory research

    pub_type: 杂志文章,多中心研究

    doi:10.1186/s12931-017-0659-x

    authors: Menéndez R,Méndez R,Polverino E,Rosales-Mayor E,Amara-Elori I,Reyes S,Posadas T,Fernández-Barat L,Torres A

    更新日期:2017-09-30 00:00:00

  • The revised ATS/ERS/JRS/ALAT diagnostic criteria for idiopathic pulmonary fibrosis (IPF)--practical implications.

    abstract::Idiopathic pulmonary fibrosis (IPF), the most prevalent idiopathic interstitial pneumonia, is associated with a poor prognosis. An accurate diagnosis of IPF is essential for optimal management. The recent ATS/ERS/JRS/ALAT recommendations on the diagnosis and management of IPF were developed from a systematic review of...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-14-S1-S2

    authors: Wells AU

    更新日期:2013-01-01 00:00:00

  • Use of Berlin questionnaire in comparison to polysomnography and home sleep study in patients with obstructive sleep apnea.

    abstract:BACKGROUND:Obstructive sleep apnea syndrome (OSAS) is a common disorder with significant morbidity and mortality. We aimed to evaluate the predictive accuracy of the Berlin questionnaire in patients with suspected OSAS undergoing PSG in the sleep laboratory setting against those going through the Embletta™ portable dia...

    journal_title:Respiratory research

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/s12931-019-1009-y

    authors: Ng SS,Tam W,Chan TO,To KW,Ngai J,Chan KKP,Yip WH,Lo RL,Yiu K,Ko FW,Hui DS

    更新日期:2019-02-22 00:00:00

  • Sialic acid receptor detection in the human respiratory tract: evidence for widespread distribution of potential binding sites for human and avian influenza viruses.

    abstract:BACKGROUND:Influenza virus binds to cell receptors via sialic acid (SA) linked glycoproteins. They recognize SA on host cells through their haemagglutinins (H). The distribution of SA on cell surfaces is one determinant of host tropism and understanding its expression on human cells and tissues is important for underst...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-8-73

    authors: Nicholls JM,Bourne AJ,Chen H,Guan Y,Peiris JS

    更新日期:2007-10-25 00:00:00

  • Relationship between age and bronchodilator response at diagnosis in adult-onset asthma.

    abstract:BACKGROUND:Possible variation in bronchodilator response (BDR) according to age at the diagnosis of adult-onset asthma is unknown. Our aim was to assess if BDR in FEV1 is related to age at diagnosis of adult-onset asthma and how many subjects fulfill the 400 mL criterion of BDR, the suggested cut-off for asthma-like re...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/s12931-020-01441-w

    authors: Tommola M,Won HK,Ilmarinen P,Jung H,Tuomisto LE,Lehtimäki L,Niemelä O,Kim TB,Kankaanranta H

    更新日期:2020-07-13 00:00:00

  • Association of mast cells with lung function in chronic obstructive pulmonary disease.

    abstract:BACKGROUND:In asthma, higher chymase positive mast cell (MC-C) numbers are associated with less airway obstruction. In COPD, the distribution of MC-C and tryptase positive mast cells (MC-T) in central and peripheral airways, and their relation with lung function, is unknown. We compared MC-T and MC-C distributions in C...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-9-64

    authors: Gosman MM,Postma DS,Vonk JM,Rutgers B,Lodewijk M,Smith M,Luinge MA,Ten Hacken NH,Timens W

    更新日期:2008-09-10 00:00:00

  • Transposable elements and their potential role in complex lung disorder.

    abstract::Transposable elements (TEs) are a class of mobile genetic elements (MGEs) that were long regarded as junk DNA, which make up approximately 45% of the genome. Although most of these elements are rendered inactive by mutations and other gene silencing mechanisms, TEs such as long interspersed nuclear elements (LINEs) ar...

    journal_title:Respiratory research

    pub_type: 杂志文章,评审

    doi:10.1186/1465-9921-14-99

    authors: Sargurupremraj M,Wjst M

    更新日期:2013-10-05 00:00:00

  • The unique alterations of hippocampus and cognitive impairment in chronic obstructive pulmonary disease.

    abstract:BACKGROUND:Cognitive impairment has been found in chronic obstructive pulmonary disease (COPD) patients. However, the structural alteration of the brain and underlying mechanisms are poorly understood. METHODS:Thirty-seven mild-to-moderate COPD patients, forty-eight severe COPD patients, and thirty-one control subject...

    journal_title:Respiratory research

    pub_type: 杂志文章

    doi:10.1186/1465-9921-14-140

    authors: Li J,Fei GH

    更新日期:2013-12-21 00:00:00

  • Viruses, dendritic cells and the lung.

    abstract::The interaction between viruses and dendritic cells (DCs) is varied and complex. DCs are key elements in the development of a host response to pathogens such as viruses, but viruses have developed survival tactics to either evade or diminish the immune system that functions to kill and eliminate these micro-organisms....

    journal_title:Respiratory research

    pub_type: 杂志文章,评审

    doi:10.1186/rr63

    authors: Peebles RS Jr,Graham BS

    更新日期:2001-01-01 00:00:00

  • Marketing data: has the rise of impact factor led to the fall of objective language in the scientific article?

    abstract::The language of science should be objective and detached and should place data in the appropriate context. The aim of this commentary was to explore the notion that recent trends in the use of language have led to a loss of objectivity in the presentation of scientific data. The relationship between the value-laden vo...

    journal_title:Respiratory research

    pub_type: 社论

    doi:10.1186/1465-9921-10-35

    authors: Fraser VJ,Martin JG

    更新日期:2009-05-11 00:00:00

  • Psychometric properties and minimal important differences of SF-36 in Idiopathic Pulmonary Fibrosis.

    abstract:BACKGROUND:Idiopathic pulmonary fibrosis (IPF) is a rare disease with a median survival of 3-5 years after diagnosis with limited treatment options. The aim of this study is to assess the psychometric characteristics of the Short Form 36 Health Status Questionnaire (SF-36) in IPF and to provide disease specific minimal...

    journal_title:Respiratory research

    pub_type: 临床试验,杂志文章

    doi:10.1186/s12931-019-1010-5

    authors: Witt S,Krauss E,Barbero MAN,Müller V,Bonniaud P,Vancheri C,Wells AU,Vasakova M,Pesci A,Klepetko W,Seeger W,Crestani B,Leidl R,Holle R,Schwarzkopf L,Guenther A

    更新日期:2019-03-01 00:00:00

  • Abstracts from the 2018 Respiratory Effectiveness Group Summit : Amsterdam, the Netherlands. 22-24 March 2018.

    abstract:: ...

    journal_title:Respiratory research

    pub_type:

    doi:10.1186/s12931-018-0930-9

    authors:

    更新日期:2018-12-20 00:00:00