Abstract:
OBJECTIVES:To assess the structure and extracellular matrix molecule expression of osteogenic cell sheets created via culture in medium with both dexamethasone (Dex) and ascorbic acid phosphate (AscP) compared either Dex or AscP alone. METHODS:Osteogenic cell sheets were prepared by culturing rat bone marrow stromal cells in a minimal essential medium (MEM), MEM with AscP, MEM with Dex, and MEM with Dex and AscP (Dex/AscP). The cell number and messenger (m)RNA expression were assessed in vitro, and the appearance of the cell sheets was observed after mechanical retrieval using a scraper. β-tricalcium phosphate (β-TCP) was then wrapped with the cell sheets from the four different groups and subcutaneously implanted into rats. RESULTS:After mechanical retrieval, the osteogenic cell sheets from the MEM, MEM with AscP, and MEM with Dex groups appeared to be fragmented or incomplete structures. The cell sheets cultured with Dex/AscP remained intact after mechanical retrieval, without any identifiable tears. Culture with Dex/AscP increased the mRNA and protein expression of extracellular matrix proteins and cell number compared with those of the other three groups. More bridging bone formation was observed after transplantation of the β-TCP scaffold wrapped with cell sheets cultured with Dex/AscP, than in the other groups. CONCLUSIONS:These results suggest that culture with Dex/AscP improves the mechanical integrity of the osteogenic cell sheets, allowing retrieval of the confluent cells in a single cell sheet structure. This method may be beneficial when applied in cases of difficult tissue reconstruction, such as nonunion, bone defects, and osteonecrosis.Cite this article: M. Akahane, T. Shimizu, T. Kira, T. Onishi, Y. Uchihara, T. Imamura, Y. Tanaka. Culturing bone marrow cells with dexamethasone and ascorbic acid improves osteogenic cell sheet structure. Bone Joint Res 2016;5:569-576. DOI: 10.1302/2046-3758.511.BJR-2016-0013.R1.
journal_name
Bone Joint Resjournal_title
Bone & joint researchauthors
Akahane M,Shimizu T,Kira T,Onishi T,Uchihara Y,Imamura T,Tanaka Ydoi
10.1302/2046-3758.511.BJR-2016-0013.R1subject
Has Abstractpub_date
2016-11-01 00:00:00pages
569-576issue
11issn
2046-3758pii
5/11/569journal_volume
5pub_type
杂志文章abstract:AIMS:Treatment of chronic osteomyelitis (COM) for young patients remains a challenge. Large bone deficiencies secondary to COM can be treated using induced membrane technique (IMT). However, it is unclear which type of bone graft is optimal. The goal of the study was to determine the clinical effectiveness of bone marr...
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abstract:OBJECTIVES:Microindentation has the potential to measure the stiffness of an individual patient's bone. Bone stiffness plays a crucial role in the press-fit stability of orthopaedic implants. Arming surgeons with accurate bone stiffness information may reduce surgical complications including periprosthetic fractures. T...
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更新日期:2019-11-02 00:00:00
abstract:OBJECTIVES:In order to ensure safety of the cell-based therapy for bone regeneration, we examined in vivo biodistribution of locally or systemically transplanted osteoblast-like cells generated from bone marrow (BM) derived mononuclear cells. METHODS:BM cells obtained from a total of 13 Sprague-Dawley (SD) green fluor...
journal_title:Bone & joint research
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journal_title:Bone & joint research
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更新日期:2018-05-05 00:00:00
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更新日期:2018-09-15 00:00:00
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更新日期:2015-05-01 00:00:00
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更新日期:2020-01-08 00:00:00
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journal_title:Bone & joint research
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更新日期:2014-03-19 00:00:00
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更新日期:2019-12-03 00:00:00
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journal_title:Bone & joint research
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更新日期:2014-03-10 00:00:00
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更新日期:2012-06-01 00:00:00
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journal_title:Bone & joint research
pub_type: 杂志文章
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更新日期:2012-11-01 00:00:00
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更新日期:2017-05-01 00:00:00
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更新日期:2020-11-01 00:00:00