Abstract:
BACKGROUND:Cigarette smoke induced oxidative stress has been shown to reduce silent information regulator 1 (Sirt1) levels in lung tissue from smokers and patients with COPD patients. Sirt1 is known to inhibit endothelial senescence and may play a protective role in vascular cells. Endothelial progenitor cells (EPCs) are mobilized into circulation under various pathophysiological conditions, and are thought to play an important role in tissue repair in chronic obstructive lung disease (COPD). Therefore, Sirt1 and EPC-associated mRNAs were measured in blood samples from patients with COPD and from cultured CD34+ progenitor cells to examine whether these genes are associated with COPD development. METHODS:This study included 358 patients with a smoking history of more than 10 pack-years. RNA was extracted from blood samples and from CD34+ progenitor cells treated with cigarette smoke extract (CSE), followed by assessment of CD31, CD34, Sirt1 mRNA, miR-34a, and miR-126-3p expression by real-time RT-PCR. RESULTS:The expression of CD31, CD34, Sirt1 mRNAs, and miR-126-3p decreased and that of miR-34a increased in moderate COPD compared with that in control smokers. However, no significant differences in these genes were observed in blood cells from patients with severe COPD compared with those in control smokers. CSE significantly decreased Sirt1 and increased miR-34a expression in cultured progenitor cells. CONCLUSION:Sirt1 expression in blood cells from patients with COPD could be a biomarker for disease stability in patients with moderate COPD. MiR-34a may participate in apoptosis and/or senescence of EPCs in smokers. Decreased expression of CD31, CD34, and miR-126-3p potentially represents decreased numbers of EPCs in blood cell from patients with COPD.
journal_name
Respir Resjournal_title
Respiratory researchauthors
Kato R,Mizuno S,Kadowaki M,Shiozaki K,Akai M,Nakagawa K,Oikawa T,Iguchi M,Osanai K,Ishizaki T,Voelkel NF,Toga Hdoi
10.1186/s12931-016-0452-2subject
Has Abstractpub_date
2016-10-27 00:00:00pages
139issue
1eissn
1465-9921issn
1465-993Xpii
10.1186/s12931-016-0452-2journal_volume
17pub_type
杂志文章abstract:BACKGROUND:The acute respiratory distress syndrome (ARDS) is characterized by the acute onset of hypoxemia and bilateral lung infiltrates in response to an inciting event, and is associated with high morbidity and mortality. Patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT) are at increased ...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-019-0981-6
更新日期:2019-01-21 00:00:00
abstract:BACKGROUND:CpG oligodeoxynucleotides (CpG-ODN) are capable of inducing high amounts of type I IFNs with many immunomodulatory properties. Furthermore, type-I IFNs have been proposed to play a key role in mediating effects of CpG-ODN. The precise role of IFN-beta in the immunomodulatory effects of CpG-ODN is not known. ...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-6-25
更新日期:2005-03-05 00:00:00
abstract:BACKGROUND:Exhaled nitric oxide (F(E)NO) measurements are used as a surrogate marker for eosinophilic airway inflammation. However, many constitutional and environmental factors affect F(E)NO, making it difficult to devise reference values. Our aim was to evaluate the relative importance of factors affecting F(E)NO in ...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-8-82
更新日期:2007-11-15 00:00:00
abstract::The transcription factors nuclear factor interleukin-6 (NF-IL6), early growth response-1 (EGR-1) and hypoxia-inducible factor-1 (HIF-1) have important roles in the molecular pathophysiology of hypoxia-associated pulmonary disease. NF-IL6 controls the production of interleukin (IL)-6 in vascular endothelial cells, whic...
journal_title:Respiratory research
pub_type: 杂志文章,评审
doi:10.1186/rr27
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND:While glucocorticoids are currently the most effective therapy for asthma, associated side effects limit enthusiasm for their use. Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) activators include the synthetic thiazolidinediones (TZDs) which exhibit anti-inflammatory effects that suggest usef...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-8-90
更新日期:2007-12-04 00:00:00
abstract::The airway epithelium forms a barrier against infection but also produces antimicrobial peptides (AMPs) and other inflammatory mediators to activate the immune system. It has been shown that in allergic disorders, Th2 cytokines may hamper the antimicrobial activity of the epithelium. However, the presence of Th2 cytok...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-12-59
更新日期:2011-05-02 00:00:00
abstract:BACKGROUND:Impaired skeletal muscle regeneration could contribute to the progression of muscle atrophy in patients with chronic obstructive pulmonary disease (COPD). METHODS:Satellite cells and myogenesis-related proteins were compared between healthy subjects and patients with COPD, with or without muscle atrophy. Sa...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-15-35
更新日期:2014-03-25 00:00:00
abstract:RATIONALE:Information concerning how climate and atmospheric pollutants affects physical activity in COPD patients is lacking and might be valuable in determining when physical activity should be encouraged. METHODS:Seventy-three stable COPD patients recorded on daily diary cards worsening of respiratory symptoms, pea...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-015-0229-z
更新日期:2015-06-13 00:00:00
abstract:BACKGROUND:Colonization of the airways with potential pathogenic bacteria is observed in a number of chronic respiratory diseases, such as COPD or cystic fibrosis. Infections with respiratory viruses are known triggers of exacerbations of these diseases. We here investigated if pre-exposure to bacteria alters the respo...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-016-0382-z
更新日期:2016-06-03 00:00:00
abstract::By airway surface liquid, we mean a thin fluid continuum consisting of the airway lining layer and the alveolar lining layer, which not only serves as a protective barrier against foreign particles but also contributes to maintaining normal respiratory mechanics. In recent years, measurements of the rheological proper...
journal_title:Respiratory research
pub_type: 杂志文章,评审
doi:10.1186/s12931-019-1229-1
更新日期:2019-12-04 00:00:00
abstract:BACKGROUND:Manual auscultation to detect abnormal breath sounds has poor inter-observer reliability. Digital stethoscopes with artificial intelligence (AI) could improve reliable detection of these sounds. We aimed to independently test the abilities of AI developed for this purpose. METHODS:One hundred and ninety two...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-020-01523-9
更新日期:2020-09-29 00:00:00
abstract:BACKGROUND:The pathogenesis of allergic airway inflammation in asthmatic patients is complex and characterized by cellular infiltrates and activity of many cytokines and chemokines. Both the transcription factor hypoxia inducible factor-1 (HIF-1) and chemokine CCL2 have been shown to play pivotal roles in allergic airw...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-13-60
更新日期:2012-07-23 00:00:00
abstract:BACKGROUND:Acute respiratory failure (ARF) and severe sepsis (SS) are possible complications in patients with community-acquired pneumonia (CAP). The aim of the study was to evaluate prevalence, characteristics, risk factors and impact on mortality of hospitalized patients with CAP according to the presence of ARF and ...
journal_title:Respiratory research
pub_type: 杂志文章,多中心研究
doi:10.1186/1465-9921-15-27
更新日期:2014-03-04 00:00:00
abstract::Despite much information on their catalytic properties and gene regulation, we actually know very little of what matrix metalloproteinases (MMPs) do in tissues. The catalytic activity of these enzymes has been implicated to function in normal lung biology by participating in branching morphogenesis, homeostasis, and r...
journal_title:Respiratory research
pub_type: 杂志文章,评审
doi:10.1186/rr33
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Pulmonary fibrotic diseases induce significant morbidity and mortality, for which there are limited therapeutic options available. Rac2, a ras-related guanosine triphosphatase expressed mainly in hematopoietic cells, is a crucial molecule regulating a diversity of mast cell, macrophage, and neutrophil functi...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-15-71
更新日期:2014-06-27 00:00:00
abstract:BACKGROUND:Mutations in the eukaryotic translation initiation factor 2α kinase 4 (EIF2AK4) gene have recently been identified in recessively inherited veno-occlusive disease. In this study we assessed if EIF2AK4 mutations occur also in a family with autosomal dominantly inherited pulmonary arterial hypertension (HPAH) ...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-016-0457-x
更新日期:2016-11-04 00:00:00
abstract:BACKGROUND:The aim of this study was to develop and validate a model for pulmonary fibrosis, using ex vivo tissue cultures of lungs from bleomycin treated animals, enabling the investigation of fibrosis remodeling using novel biomarkers for the detection of ECM protein fragments. The combination of in vivo and ex vivo ...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-016-0394-8
更新日期:2016-07-05 00:00:00
abstract:BACKGROUND:Functional deterioration in cystic fibrosis (CF) may be reflected by increasing bronchial obstruction and, as recently shown, by ventilation inhomogeneities. This study investigated which physiological factors (airway obstruction, ventilation inhomogeneities, pulmonary hyperinflation, development of trapped ...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-7-138
更新日期:2006-11-30 00:00:00
abstract::Respiratory diseases including asthma and chronic obstructive pulmonary disease (COPD) are characterised by excessive and persistent inflammation. Current treatments are often inadequate for symptom and disease control, and hence new therapies are warranted. Recent emerging research has implicated dyslipidaemia in pul...
journal_title:Respiratory research
pub_type: 杂志文章,评审
doi:10.1186/s12931-017-0526-9
更新日期:2017-02-28 00:00:00
abstract:BACKGROUND:Several studies have implicated a role of inflammation in the pathogenesis of lung damage in idiopathic pulmonary fibrosis (IPF). Parenchymal lung damage leads to defects in mechanics and gas exchange and clinically manifests with exertional dyspnea. Investigations of inflammatory cells in IPF have shown tha...
journal_title:Respiratory research
pub_type: 临床试验,杂志文章
doi:10.1186/1465-9921-6-81
更新日期:2005-07-24 00:00:00
abstract:BACKGROUND:Antigen desensitization through oral tolerance is becoming an increasingly attractive treatment option for allergic diseases. However, the mechanism(s) by which tolerization is achieved remain poorly defined. In this study we endeavored to induce oral tolerance to cockroach allergen (CRA: a complex mixture o...
journal_title:Respiratory research
pub_type: 杂志文章,随机对照试验
doi:10.1186/1465-9921-11-160
更新日期:2010-11-23 00:00:00
abstract:BACKGROUND:To compare the efficacy and safety of budesonide/formoterol (Symbicort) with formoterol (Oxis) in the treatment of patients with acute asthma who showed evidence of refractoriness to short-acting beta2-agonist therapy. METHODS:In a 3 hour, randomized, double-blind study, a total of 115 patients with acute a...
journal_title:Respiratory research
pub_type: 杂志文章,随机对照试验
doi:10.1186/1465-9921-7-13
更新日期:2006-01-24 00:00:00
abstract:BACKBROUND:COPD is a common, highly debilitating disease of the airways, primarily caused by smoking. Chronic inflammation and structural remodelling are key pathological features of this disease caused, in part, by the aberrant function of airway smooth muscle (ASM). We have previously demonstrated that hydrogen sulfi...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-018-0788-x
更新日期:2018-05-09 00:00:00
abstract:BACKGROUND:Stress of the endoplasmic reticulum (ER) leading to activation of the unfolded protein response (UPR) and alveolar epithelial cell (AEC) apoptosis may play a role in the pathogenesis of idiopathic pulmonary fibrosis (IPF). Our objectives were to determine whether circulating caspase-cleaved cytokeratin-18 (c...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-13-105
更新日期:2012-11-20 00:00:00
abstract:BACKGROUND:Long-term lung allograft survival is limited by bronchiolitis obliterans syndrome (BOS). Mannose binding lectin (MBL) belongs to the innate immune system, participates in complement activation, and may predispose to graft rejection. We investigated mannose binding (MBL) during cold ischemia and in tissue sam...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-13-56
更新日期:2012-07-04 00:00:00
abstract:RESEARCH QUESTION:There is no widely accepted grading system for IPF disease severity, although physiologic impairment based on pulmonary function testing is frequently employed. We sought to describe clinical and functional characteristics as well as outcomes of patients with severe physiologic impairment. PATIENTS A...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-020-01600-z
更新日期:2021-01-06 00:00:00
abstract:BACKGROUND:Growing evidences suggested that the Mycobacterium tuberculosis complex (MTBC) lineages can determine the clinical outcome of pulmonary and extra-pulmonary tuberculosis. However, limited data only available revealing such association of bacterial genotypes and clinical phenotypes from immigrant rich countrie...
journal_title:Respiratory research
pub_type: 杂志文章,多中心研究
doi:10.1186/s12931-018-0966-x
更新日期:2018-12-27 00:00:00
abstract:BACKGROUND:Early life impairments leading to lower lung function by adulthood are considered as risk factors for chronic obstructive pulmonary disease (COPD). Recently, we compared the lung transcriptomic profile between two mouse strains with extreme total lung capacities to identify plausible pulmonary function deter...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-018-0795-y
更新日期:2018-06-06 00:00:00
abstract::In the present study, we used tumor necrosis factor-R1 knock out mice (TNF-alpha R1KO) to understand the roles of TNF-alpha on epithelial function in models of carrageenan-induced acute lung inflammation. In order to elucidate whether the observed anti-inflammatory status is related to the inhibition of TNF-alpha, we ...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-8-75
更新日期:2007-10-30 00:00:00
abstract:BACKGROUND:To assess the symptomatic and cost burden among patients initiating long-acting bronchodilator (LABD) therapy and impact of adherence on healthcare resource use and costs. METHODS:This retrospective cohort study identified patients with COPD who were newly prescribed a LABD (long-acting muscarinic antagonis...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-015-0295-2
更新日期:2015-11-16 00:00:00