Assessment of tissue oxygen tension: comparison of dynamic fluorescence quenching and polarographic electrode technique.

Abstract:

INTRODUCTION AND METHODS:Dynamic fluorescence quenching is a technique that may overcome some of the limitations associated with measurement of tissue partial oxygen tension (PO2). We compared this technique with a polarographic Eppendorf needle electrode method using a saline tonometer in which the PO2 could be controlled. We also tested the fluorescence quenching system in a rodent model of skeletal muscle ischemiahypoxia. RESULTS:Both systems measured PO2 accurately in the tonometer, and there was excellent correlation between them (r(2) = 0.99). The polarographic system exhibited proportional bias that was not evident with the fluorescence method. In vivo, the fluorescence quenching technique provided a readily recordable signal that varied as expected. DISCUSSION:Measurement of tissue PO2 using fluorescence quenching is at least as accurate as measurement using the Eppendorf needle electrode in vitro, and may prove useful in vivo for assessment of tissue oxygenation.

journal_name

Crit Care

authors

Shaw AD,Li Z,Thomas Z,Stevens CW

doi

10.1186/cc1457

keywords:

subject

Has Abstract

pub_date

2002-02-01 00:00:00

pages

76-80

issue

1

eissn

1364-8535

issn

1466-609X

journal_volume

6

pub_type

杂志文章
  • Life-threatening massive pulmonary embolism rescued by venoarterial-extracorporeal membrane oxygenation.

    abstract:BACKGROUND:Despite quick implementation of reperfusion therapies, a few patients with high-risk, acute, massive, pulmonary embolism (PE) remain highly hemodynamically unstable. Others have absolute contraindication to receive reperfusion therapies. Venoarterial-extracorporeal membrane oxygenation (VA-ECMO) might lower ...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章

    doi:10.1186/s13054-017-1655-8

    authors: Corsi F,Lebreton G,Bréchot N,Hekimian G,Nieszkowska A,Trouillet JL,Luyt CE,Leprince P,Chastre J,Combes A,Schmidt M

    更新日期:2017-03-28 00:00:00

  • Prediction of fluid challenge effect: filling pressure when left ventricular function is abnormal, diastolic volume when left ventricular function is normal.

    abstract::Fluid resuscitation is a cornerstone of intensive care unit patient care, but prediction of the cardiovascular response remains difficult, despite many efforts in clinical research. The concept of responders and nonresponders illustrates such a difficulty. Many techniques have been tested, from strictly non-invasive t...

    journal_title:Critical care (London, England)

    pub_type: 评论,杂志文章

    doi:10.1186/cc10067

    authors: Payen D

    更新日期:2011-01-01 00:00:00

  • Bench-to-bedside review: fulfilling promises of the Human Genome Project.

    abstract::Since most common diseases have been shown to be influenced by inherited variations in our genes, completion of the Human Genome Project and mapping of the human genome single-nucleotide polymorphisms will have a tremendous impact on our approach to medicine. New developments in genotyping techniques and bioinformatic...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章,评审

    doi:10.1186/cc1491

    authors: Chiche JD,Cariou A,Mira JP

    更新日期:2002-06-01 00:00:00

  • Inter-hospital transport of critically ill patients; expect surprises.

    abstract:INTRODUCTION:Inter-hospital transport of critically ill patients is increasing. When performed by specialized retrieval teams there are less adverse events compared to transport by ambulance. These transports are performed with technical equipment also used in an Intensive Care Unit (ICU). As a consequence technical pr...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章

    doi:10.1186/cc11191

    authors: Droogh JM,Smit M,Hut J,de Vos R,Ligtenberg JJ,Zijlstra JG

    更新日期:2012-02-12 00:00:00

  • Retrospective cohort study of hospital variation in airway management during in-hospital cardiac arrest and the association with patient survival: insights from Get With The Guidelines-Resuscitation.

    abstract:IMPORTANCE:The optimal approach to airway management during in-hospital cardiac arrest is unknown. OBJECTIVE:To describe hospital-level variation in endotracheal intubation during cardiopulmonary resuscitation (CPR) for in-hospital cardiac arrest and the association between hospital use of endotracheal intubation and ...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章

    doi:10.1186/s13054-019-2426-5

    authors: Bradley SM,Zhou Y,Ramachandran SK,Engoren M,Donnino M,Girotra S

    更新日期:2019-05-06 00:00:00

  • The International Sepsis Forum's controversies in sepsis: corticosteroids should be used to treat septic shock.

    abstract::The use of corticosteroids in septic shock remains controversial. It has been demonstrated that high doses of steroids (30 mg/kg methylprednisolone) for short periods of time are not beneficial. More recent studies using smaller doses (200-300 mg/day hydrocortisone) for longer periods of time have shown beneficial eff...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章,评审

    doi:10.1186/cc1537

    authors: Goodman S,Sprung CL,International Sepsis Forum.

    更新日期:2002-10-01 00:00:00

  • G-CSF and IL-8 for early diagnosis of sepsis in neonates and critically ill children - safety and cost effectiveness of a new laboratory prediction model: study protocol of a randomized controlled trial [ISRCTN91123847].

    abstract:INTRODUCTION:Bacterial infection represents a serious risk in neonates and critically ill paediatric patients. Current clinical practice is characterized by frequent antibiotic treatment despite low incidence of true infection. However, some patients escape early diagnosis and progress to septic shock. Many new markers...

    journal_title:Critical care (London, England)

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1186/cc2971

    authors: Horisberger T,Harbarth S,Nadal D,Baenziger O,Fischer JE

    更新日期:2004-12-01 00:00:00

  • Rapid response systems: are they really effective?

    abstract::This article is one of ten reviews selected from the Annual Update in Intensive Care and Emergency Medicine 2015 and co-published as a series in Critical Care. Other articles in the series can be found online at http://ccforum.com/series/annualupdate2015. Further information about the Annual Update in Intensive Care a...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章,评审

    doi:10.1186/s13054-015-0807-y

    authors: Sandroni C,D'Arrigo S,Antonelli M

    更新日期:2015-03-16 00:00:00

  • Success and complications by team composition for prehospital paediatric intubation: a systematic review and meta-analysis.

    abstract:BACKGROUND:Clinical team composition for prehospital paediatric intubation may affect success and complication rates. We performed a systematic review and meta-analysis to determine the success and complication rates by type of clinical team. METHODS:We searched MEDLINE, EMBASE, and CINAHL for interventional and obser...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章,meta分析

    doi:10.1186/s13054-020-02865-y

    authors: Garner AA,Bennett N,Weatherall A,Lee A

    更新日期:2020-04-15 00:00:00

  • Long-term survival after intensive care unit discharge in Thailand: a retrospective study.

    abstract:INTRODUCTION:Economic evaluations of interventions in the hospital setting often rely on the estimated long-term impact on patient survival. Estimates of mortality rates and long-term outcomes among patients discharged alive from the intensive care unit (ICU) are lacking from lower- and middle-income countries. This st...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章

    doi:10.1186/cc13036

    authors: Luangasanatip N,Hongsuwan M,Lubell Y,Limmathurotsakul D,Teparrukkul P,Chaowarat S,Day NP,Graves N,Cooper BS

    更新日期:2013-10-03 00:00:00

  • Impact of whole-body computed tomography on mortality and surgical management of severe blunt trauma.

    abstract:INTRODUCTION:The mortality benefit of whole-body computed tomography (CT) in early trauma management remains controversial and poorly understood. The objective of this study was to assess the impact of whole-body CT compared with selective CT on mortality and management of patients with severe blunt trauma. METHODS:Th...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章

    doi:10.1186/cc11375

    authors: Yeguiayan JM,Yap A,Freysz M,Garrigue D,Jacquot C,Martin C,Binquet C,Riou B,Bonithon-Kopp C,FIRST Study Group.

    更新日期:2012-06-11 00:00:00

  • Gut instinct.

    abstract::Barriers to the use of selective digestive decontamination include concerns about emergence of resistant organisms, over-estimation of current performance in preventing ventilator-associated pneumonia (VAP), alternative methods of preventing VAP, and misunderstanding of mechanisms of action. A definitive cluster-rando...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章

    doi:10.1186/cc13165

    authors: Bion J

    更新日期:2013-12-19 00:00:00

  • Bench-to-bedside review: potential strategies to protect or reverse mitochondrial dysfunction in sepsis-induced organ failure.

    abstract::The pathogenesis of sepsis-induced multiple organ failure may crucially depend on the development of mitochondrial dysfunction and consequent cellular energetic failure. According to this hypothesis, interventions aimed at preventing or reversing mitochondrial damage may have major clinical relevance, although the tim...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章,评审

    doi:10.1186/cc5014

    authors: Protti A,Singer M

    更新日期:2006-01-01 00:00:00

  • A week seems to be weak: tailoring duration of antibiotic treatment in Gram-negative ventilator-associated pneumonia.

    abstract::The optimal length of antimicrobial therapy has not been extensively studied for a great majority of infections and, in critically ill patients affected by ventilator-associated pneumonia, is a persisting and unsolved issue confronting clinicians. The integration of biomarkers, clinical judgment, and microbiologic era...

    journal_title:Critical care (London, England)

    pub_type: 评论,杂志文章

    doi:10.1186/cc11899

    authors: Martin-Loeches I,Torres A

    更新日期:2013-01-21 00:00:00

  • Induction of Bim and Bid gene expression during accelerated apoptosis in severe sepsis.

    abstract:INTRODUCTION:In transgenic animal models of sepsis, members of the Bcl-2 family of proteins regulate lymphocyte apoptosis and survival of sepsis. This study investigates the gene regulation of pro-apoptotic and anti-apoptotic members of the Bcl-2 family of proteins in patients with early stage severe sepsis. METHODS:I...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章

    doi:10.1186/cc7088

    authors: Weber SU,Schewe JC,Lehmann LE,Müller S,Book M,Klaschik S,Hoeft A,Stüber F

    更新日期:2008-01-01 00:00:00

  • Circulating bioactive adrenomedullin as a marker of sepsis, septic shock and critical illness.

    abstract:BACKGROUND:Biomarkers can be of help to understand critical illness and to identify and stratify sepsis. Adrenomedullin is a vasoactive hormone, with reported prognostic and potentially therapeutic value in sepsis. The primary aim of this study was to investigate the association of circulating bioactive adrenomedullin ...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章

    doi:10.1186/s13054-020-03351-1

    authors: Lundberg OHM,Lengquist M,Spångfors M,Annborn M,Bergmann D,Schulte J,Levin H,Melander O,Frigyesi A,Friberg H

    更新日期:2020-11-04 00:00:00

  • Reverse shock index multiplied by Glasgow Coma Scale score (rSIG) is a simple measure with high discriminant ability for mortality risk in trauma patients: an analysis of the Japan Trauma Data Bank.

    abstract:BACKGROUND:The shock index (SI), defined as heart rate (HR) divided by systolic blood pressure (SBP), is reported to be a more sensitive marker of shock than traditional vital signs alone. In previous literature, use of the reverse shock index (rSI), taken as SBP divided by HR, is recommended instead of SI for hospital...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章,多中心研究

    doi:10.1186/s13054-018-2014-0

    authors: Kimura A,Tanaka N

    更新日期:2018-04-11 00:00:00

  • Does anesthetic provide similar neuroprotection to therapeutic hypothermia after cardiac arrest?

    abstract::In the previous issue of Critical Care, Meybohm and colleagues provide evidence to support hypothermia as a kind of therapeutic option for patients suffering cardiac arrest. Although anesthetics had been used to induce hypothermia, sevoflurane post-conditioning fails to confer additional anti-inflammatory effects afte...

    journal_title:Critical care (London, England)

    pub_type: 评论,杂志文章

    doi:10.1186/cc8923

    authors: Zhang H

    更新日期:2010-01-01 00:00:00

  • Hypotension and hypovolemia during hemodialysis: is the usual suspect innocent?

    abstract::Hypotension during intermittent hemodialysis is common, and has been attributed to acute volume shifts, shifts in osmolarity, electrolyte imbalance, temperature changes, altered vasoregulation, and sheer hypovolemia. Although hypovolemia may intuitively seem a likely cause for hypotension in intensive care patients, i...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章

    doi:10.1186/s13054-016-1307-4

    authors: Berger D,Takala J

    更新日期:2016-06-09 00:00:00

  • Treatment of atelectasis: where is the evidence?

    abstract::Lobar atelectasis is a common problem caused by a variety of mechanisms including resorption atelectasis due to airway obstruction, passive atelectasis from hypoventilation, compressive atelectsis from abdominal distension and adhesive atelectasis due to increased surface tension. However, evidence-based studies on th...

    journal_title:Critical care (London, England)

    pub_type: 评论,杂志文章

    doi:10.1186/cc3766

    authors: Schindler MB

    更新日期:2005-08-01 00:00:00

  • Plasma thioredoxin levels during post-cardiac arrest syndrome: relationship with severity and outcome.

    abstract:INTRODUCTION:Despite experimental evidence, clinical demonstration of acute state of oxidative stress and inflammation during post-cardiac arrest syndrome is lacking. Plasma level of thioredoxin (TRX), a redox-active protein induced under conditions of oxidative stress and inflammation, is increased in various critical...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章

    doi:10.1186/cc12492

    authors: Mongardon N,Lemiale V,Borderie D,Burke-Gaffney A,Perbet S,Marin N,Charpentier J,Pène F,Chiche JD,Mira JP,Cariou A

    更新日期:2013-01-29 00:00:00

  • Short-term outcomes of cadaveric lung transplantation in ventilator-dependent patients.

    abstract:INTRODUCTION:Survival after cadaveric lung transplantation (LTx) in respiratory failure recipients who were already dependent on ventilation support prior to transplantation is poor, with a relatively high rate of surgical mortality and morbidity. In this study, we sought to describe the short-term outcomes of bilatera...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章

    doi:10.1186/cc7989

    authors: Hsu HH,Chen JS,Ko WJ,Huang SC,Kuo SW,Huang PM,Chi NH,Chang CC,Chen RJ,Lee YC

    更新日期:2009-01-01 00:00:00

  • Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis.

    abstract:INTRODUCTION:The current shortage of accurate and readily available, validated biomarkers of disease severity in sepsis is an important limitation when attempting to stratify patients into homogeneous groups, in order to study pathogenesis or develop therapeutic interventions. The aim of the present study was to determ...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章,多中心研究

    doi:10.1186/cc5783

    authors: Bozza FA,Salluh JI,Japiassu AM,Soares M,Assis EF,Gomes RN,Bozza MT,Castro-Faria-Neto HC,Bozza PT

    更新日期:2007-01-01 00:00:00

  • Impact of intensive care unit admission during morning bedside rounds and mortality: a multi-center retrospective cohort study.

    abstract:INTRODUCTION:Recent data have suggested that patient admission during intensive care unit (ICU) morning bedside rounds is associated with less favorable outcome. We undertook the present study to explore the association between morning round-time ICU admissions and hospital mortality in a large Canadian health region. ...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章,多中心研究

    doi:10.1186/cc11329

    authors: de Souza IA,Karvellas CJ,Gibney RT,Bagshaw SM

    更新日期:2012-05-03 00:00:00

  • Associations between single nucleotide polymorphisms in the FAS pathway and acute kidney injury.

    abstract:INTRODUCTION:To determine whether single nucleotide polymorphisms (SNPs) in FAS and related genes are associated with acute kidney injury (AKI) in patients with acute respiratory distress syndrome (ARDS). METHODS:We studied 401 (Caucasian N = 310 and African-American N = 91) patients aged ≥ 13 years with ALI who enrol...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章

    doi:10.1186/s13054-015-1084-5

    authors: Bhatraju P,Hsu C,Mukherjee P,Glavan BJ,Burt A,Mikacenic C,Himmelfarb J,Wurfel M

    更新日期:2015-10-19 00:00:00

  • Incidence and outcome of invasive candidiasis in intensive care units (ICUs) in Europe: results of the EUCANDICU project.

    abstract:BACKGROUND:The objective of this study was to assess the cumulative incidence of invasive candidiasis (IC) in intensive care units (ICUs) in Europe. METHODS:A multinational, multicenter, retrospective study was conducted in 23 ICUs in 9 European countries, representing the first phase of the candidemia/intra-abdominal...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章,多中心研究

    doi:10.1186/s13054-019-2497-3

    authors: Bassetti M,Giacobbe DR,Vena A,Trucchi C,Ansaldi F,Antonelli M,Adamkova V,Alicino C,Almyroudi MP,Atchade E,Azzini AM,Carannante N,Carnelutti A,Corcione S,Cortegiani A,Dimopoulos G,Dubler S,García-Garmendia JL,Girardis

    更新日期:2019-06-14 00:00:00

  • Simvastatin for patients with acute respiratory distress syndrome: long-term outcomes and cost-effectiveness from a randomised controlled trial.

    abstract:BACKGROUND:Simvastatin therapy for patients with acute respiratory distress syndrome (ARDS) has been shown to be safe and associated with minimal adverse effects, but it does not improve clinical outcomes. The aim of this research was to report on mortality and cost-effectiveness of simvastatin in patients with ARDS at...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1186/s13054-017-1695-0

    authors: Agus A,Hulme C,Verghis RM,McDowell C,Jackson C,O'Kane CM,Laffey JG,McAuley DF

    更新日期:2017-05-17 00:00:00

  • Effects of selective β1-adrenoceptor blockade on cardiovascular and renal function and circulating cytokines in ovine hyperdynamic sepsis.

    abstract:INTRODUCTION:Activation of the sympathetic nervous system has beneficial cardiovascular effects in sepsis, but there is also evidence that sympatholytics have beneficial actions in sepsis. We therefore determined the effect of selective β1-adrenoceptor blockade on cardiac and renal function and cytokine release in ovin...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章

    doi:10.1186/s13054-014-0610-1

    authors: Calzavacca P,Lankadeva YR,Bailey SR,Bailey M,Bellomo R,May CN

    更新日期:2014-11-21 00:00:00

  • Intensive insulin treatment improves forearm blood flow in critically ill patients: a randomized parallel design clinical trial.

    abstract:INTRODUCTION:Intensive insulin treatment of critically ill patients was seen as a promising method of treatment, though recent studies showed that reducing the blood glucose level below 6 mmol/l had a detrimental outcome. The mechanisms of the effects of insulin in the critically ill are not completely understood. The ...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/cc8202

    authors: Žuran I,Poredos P,Skale R,Voga G,Gabrscek L,Pareznik R

    更新日期:2009-01-01 00:00:00

  • Mechanical ventilation with high tidal volume induces inflammation in patients without lung disease.

    abstract:INTRODUCTION:Mechanical ventilation (MV) with high tidal volumes may induce or aggravate lung injury in critical ill patients. We compared the effects of a protective versus a conventional ventilatory strategy, on systemic and lung production of tumor necrosis factor-alpha (TNF-alpha) and interleukin-8 (IL-8) in patien...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/cc8919

    authors: Pinheiro de Oliveira R,Hetzel MP,dos Anjos Silva M,Dallegrave D,Friedman G

    更新日期:2010-01-01 00:00:00