Assessment of tissue oxygen tension: comparison of dynamic fluorescence quenching and polarographic electrode technique.


INTRODUCTION AND METHODS:Dynamic fluorescence quenching is a technique that may overcome some of the limitations associated with measurement of tissue partial oxygen tension (PO2). We compared this technique with a polarographic Eppendorf needle electrode method using a saline tonometer in which the PO2 could be controlled. We also tested the fluorescence quenching system in a rodent model of skeletal muscle ischemiahypoxia. RESULTS:Both systems measured PO2 accurately in the tonometer, and there was excellent correlation between them (r(2) = 0.99). The polarographic system exhibited proportional bias that was not evident with the fluorescence method. In vivo, the fluorescence quenching technique provided a readily recordable signal that varied as expected. DISCUSSION:Measurement of tissue PO2 using fluorescence quenching is at least as accurate as measurement using the Eppendorf needle electrode in vitro, and may prove useful in vivo for assessment of tissue oxygenation.


Crit Care


Shaw AD,Li Z,Thomas Z,Stevens CW





Has Abstract


2002-02-01 00:00:00












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    abstract:BACKGROUND:Despite quick implementation of reperfusion therapies, a few patients with high-risk, acute, massive, pulmonary embolism (PE) remain highly hemodynamically unstable. Others have absolute contraindication to receive reperfusion therapies. Venoarterial-extracorporeal membrane oxygenation (VA-ECMO) might lower ...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章


    authors: Corsi F,Lebreton G,Bréchot N,Hekimian G,Nieszkowska A,Trouillet JL,Luyt CE,Leprince P,Chastre J,Combes A,Schmidt M

    更新日期:2017-03-28 00:00:00

  • Prediction of fluid challenge effect: filling pressure when left ventricular function is abnormal, diastolic volume when left ventricular function is normal.

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    journal_title:Critical care (London, England)

    pub_type: 评论,杂志文章


    authors: Payen D

    更新日期:2011-01-01 00:00:00

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    abstract::Since most common diseases have been shown to be influenced by inherited variations in our genes, completion of the Human Genome Project and mapping of the human genome single-nucleotide polymorphisms will have a tremendous impact on our approach to medicine. New developments in genotyping techniques and bioinformatic...

    journal_title:Critical care (London, England)

    pub_type: 杂志文章,评审


    authors: Chiche JD,Cariou A,Mira JP

    更新日期:2002-06-01 00:00:00

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    journal_title:Critical care (London, England)

    pub_type: 杂志文章


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    更新日期:2012-02-12 00:00:00

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    journal_title:Critical care (London, England)

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    journal_title:Critical care (London, England)

    pub_type: 临床试验,杂志文章,随机对照试验


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    更新日期:2020-04-15 00:00:00

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    journal_title:Critical care (London, England)

    pub_type: 杂志文章


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    journal_title:Critical care (London, England)

    pub_type: 杂志文章


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    journal_title:Critical care (London, England)

    pub_type: 杂志文章


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    journal_title:Critical care (London, England)

    pub_type: 杂志文章,评审


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    更新日期:2006-01-01 00:00:00

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    journal_title:Critical care (London, England)

    pub_type: 评论,杂志文章


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    更新日期:2013-01-21 00:00:00

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    journal_title:Critical care (London, England)

    pub_type: 杂志文章


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    journal_title:Critical care (London, England)

    pub_type: 杂志文章


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    journal_title:Critical care (London, England)

    pub_type: 杂志文章,多中心研究


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    更新日期:2018-04-11 00:00:00

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    journal_title:Critical care (London, England)

    pub_type: 评论,杂志文章


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    更新日期:2010-01-01 00:00:00

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    journal_title:Critical care (London, England)

    pub_type: 杂志文章


    authors: Berger D,Takala J

    更新日期:2016-06-09 00:00:00

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    journal_title:Critical care (London, England)

    pub_type: 评论,杂志文章


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    journal_title:Critical care (London, England)

    pub_type: 杂志文章


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    更新日期:2013-01-29 00:00:00

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    journal_title:Critical care (London, England)

    pub_type: 杂志文章


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    journal_title:Critical care (London, England)

    pub_type: 杂志文章,多中心研究


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    更新日期:2007-01-01 00:00:00

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    journal_title:Critical care (London, England)

    pub_type: 杂志文章,多中心研究


    authors: de Souza IA,Karvellas CJ,Gibney RT,Bagshaw SM

    更新日期:2012-05-03 00:00:00

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    journal_title:Critical care (London, England)

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    更新日期:2015-10-19 00:00:00

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    journal_title:Critical care (London, England)

    pub_type: 杂志文章,多中心研究,随机对照试验


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    更新日期:2017-05-17 00:00:00

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    journal_title:Critical care (London, England)

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    authors: Calzavacca P,Lankadeva YR,Bailey SR,Bailey M,Bellomo R,May CN

    更新日期:2014-11-21 00:00:00

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    journal_title:Critical care (London, England)

    pub_type: 杂志文章,随机对照试验


    authors: Žuran I,Poredos P,Skale R,Voga G,Gabrscek L,Pareznik R

    更新日期:2009-01-01 00:00:00

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    journal_title:Critical care (London, England)

    pub_type: 杂志文章,随机对照试验


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    更新日期:2010-01-01 00:00:00