Abstract:
BACKGROUND:The goal of the present study was to determine whether cytokines in the peripheral blood of naive NOD mice correlate with the disease process and thereby would provide a marker for monitoring disease activity. METHODS:Female NOD mice (5, 10 and 14-16 weeks of age) were investigated in a cross-sectional study. In the group of 14-16-week-old mice, non-diabetic and diabetic mice were analysed as different subgroups. The Th1 cytokine (IFN-gamma) and the Th2 cytokine (IL-10) were quantified in serum by sandwich enzyme-linked immunosorbent assay (ELISA). Pancreatic mRNA for IFN-gamma and IL-10 was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) from the same animals. RESULTS:Serum levels of IFN-gamma were initially low but increased with age in NOD mice, reaching the highest levels at diabetes onset (p<0.002 compared to 10 weeks). A similar rise was noted in IFN-gamma gene expression in pancreatic lesions. In contrast, an early peak of serum IL-10 levels was observed in non-diabetic NOD mice (10 weeks) at a stage where non-destructive insulitis occurs. With increasing age a continuous loss of IL-10 until progression towards diabetes was observed. The pancreatic IL-10 mRNA expression correlated with serum IL-10 changes. As a consequence, the ratio of IFN-gamma/IL-10, reflecting the Th1/Th2 balance in the serum, was significantly increased in diabetic compared to non-diabetic NOD mice (p<0.005). CONCLUSION:These results demonstrate, for the first time, that an increased Th2 pattern in the non-diabetic stage preceding a Th1 shift is associated with the development of diabetes in naive NOD mice. Serum cytokines correlate with disease progression and pancreatic cytokine expression during prediabetes. Soluble cytokines measured in the periphery are therefore promising surrogate markers of diabetes development.
journal_name
Diabetes Metab Res Revjournal_title
Diabetes/metabolism research and reviewsauthors
Schloot NC,Hanifi-Moghaddam P,Goebel C,Shatavi SV,Flohé S,Kolb H,Rothe Hdoi
10.1002/dmrr.256keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
64-70issue
1eissn
1520-7552issn
1520-7560pii
10.1002/dmrr.256journal_volume
18pub_type
杂志文章abstract::Diabetes is a group of metabolic diseases characterized by elevated blood glucose levels that drive the development of life-threatening complications. Diabetes results from a situation of insufficient insulin action, either by deficient production of the hormone by the pancreas, or by the development of insulin resist...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章,评审
doi:10.1002/dmrr.3107
更新日期:2019-03-01 00:00:00
abstract:BACKGROUND:HLA region is the major locus (IDDM1) of type 1 diabetes (T1D) susceptibility. It explains approximately 50% of the genetic background of T1D, indicating additional genetic determinants. Genome scans and candidate gene studies have generated several chromosomal candidate regions that may have a role in T1D d...
journal_title:Diabetes/metabolism research and reviews
pub_type: 临床试验,杂志文章
doi:10.1002/dmrr.424
更新日期:2004-03-01 00:00:00
abstract::It is well established that hyperglycaemia is associated with many negative cardiovascular and immunological effects. Because of the high prevalence of underlying vascular disease along with associated infection, patients with diabetic foot ulcers are especially vulnerable to these adverse consequences. While studies ...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章,评审
doi:10.1002/dmrr.2741
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Elevated liver enzymes are associated with cardiovascular disease, while their relationship with cancer-related mortality has not been assessed so far in diabetic patients. METHODS:An observational cohort study was performed on a consecutive series of 1952 type 2 diabetic patients. The association of liver ...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章
doi:10.1002/dmrr.744
更新日期:2007-11-01 00:00:00
abstract:BACKGROUND:fulminant type 1 diabetes mellitus (T1DM) is characterized by abrupt onset of hyperglycemia and rapid progression to ketoacidosis. This study aimed at examining the clinical and autoimmunity features of fulminant T1DM in Chinese. METHODS:a total of 24 patients with fulminant T1DM and 48 classical autoimmune...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章
doi:10.1002/dmrr.1148
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:In patients with type 1 diabetes, three main variables need to be assessed to optimize meal-related insulin boluses: pre-meal blood glucose (BG), insulin to carbohydrate ratio (I : C), and basal insulin. We are presenting data for a novel use of the hyperinsulinaemic-euglycaemic clamp (HEC) in patients with ...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章
doi:10.1002/dmrr.727
更新日期:2007-09-01 00:00:00
abstract::Around one of three diabetic patients is affected by distal symmetric polyneuropathy (DSP) which represents a major health problem, as it may present with partly excruciating neuropathic pain and is responsible for substantial morbidity and increased mortality. Treatment is based on four cornerstones: (1) multifactori...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章,评审
doi:10.1002/dmrr.817
更新日期:2008-05-01 00:00:00
abstract:BACKGROUND:The aim of this study was to assess the clinical implications of calculating an individualized HbA1c target using a recently published algorithm in a real-life clinical setting. METHODS:General practitioners (GPs) from the Spanish Society of Family Medicine Diabetes Expert Group were invited to participate ...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章
doi:10.1002/dmrr.2976
更新日期:2018-03-01 00:00:00
abstract::The United Kingdom Prospective Diabetes Study (UKPDS) showed that tight glycemic control with any of several therapeutic regimens has the potential to significantly reduce the risk for long-term microvascular complications of type 2 diabetes. An important question that remains to be answered is what is the best approa...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章,评审
doi:10.1002/dmrr.304
更新日期:2002-09-01 00:00:00
abstract:BACKGROUND:Adenosine triphosphate (ATP) is a critical determinant of beta-cell insulin secretion in response to glucose. BHE/cdb rats have a mutation in ATP synthase that limits ATP production, yet develop mild diabetes only with ageing. We investigated the cellular basis for reduced insulin secretion and compensatory ...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章
doi:10.1002/dmrr.819
更新日期:2008-07-01 00:00:00
abstract:BACKGROUND:Non-alcoholic fatty liver disease (NAFLD) can lead to cirrhosis and hepatocellular carcinoma and is strongly associated with obesity and insulin resistance. The aim of this study was to assess if plasma markers associated with NAFLD are increased in people with concomitant diabetes compared with those withou...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章
doi:10.1002/dmrr.2269
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Diabetic nephropathy (DN) is the most common cause of end stage renal failure or even death among patients with type 2 diabetes mellitus. Genetic predisposition is widely studied among these patients to identify manageable aspects of the disease pathogenesis. This study was carried out to test ...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章
doi:10.1002/dmrr.3299
更新日期:2020-07-01 00:00:00
abstract::Four kinds of subcutaneous continuous glucose monitoring (CGM) machines have been currently introduced in clinical practice. These machines exhibit real-time glucose on the monitor every 5 minutes and have alarms to indicate hypoglycaemia and hyperglycaemia. However, thus far, there is no clear consensus about the cli...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章,评审
doi:10.1002/dmrr.2346
更新日期:2012-12-01 00:00:00
abstract::The International Working Group on the Diabetic Foot (IWGDF) has published evidence-based guidelines on the prevention and management of diabetic foot disease since 1999. In conjunction with advice from internal and external reviewers and expert consultants in the field, this update is based on a systematic review of ...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章,评审
doi:10.1002/dmrr.3283
更新日期:2020-03-01 00:00:00
abstract:BACKGROUND:Visceral adiposity index (VAI) is a novel marker of visceral adipose tissue accumulation and dysfunction. The study aim was to explore the association of VAI with the 10-year type 2 diabetes mellitus (T2DM) incidence in apparently healthy individuals and compare its T2DM predictive ability against common ant...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章
doi:10.1002/dmrr.3161
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND:Smooth muscle cells proliferation and extracellular matrix (ECM) protein deposition are key features of diabetic macroangiopathy. In the present study, we have studied the role of endothelin(A) (ET(A)) receptor, the predominant receptor on smooth muscle cells, in diabetes-induced vascular hypertrophy and rem...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章
doi:10.1002/dmrr.527
更新日期:2005-07-01 00:00:00
abstract::The prevalence of diabetes mellitus in primary hyperparathyroidism is approximately 8% and that of primary hyperparathyroidism in diabetic patients is approximately 1%. Both values are about three-fold higher than the respective expected prevalences in general populations. Patients with both disorders are over 40 year...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章,评审
doi:10.1002/dmrr.199
更新日期:2001-05-01 00:00:00
abstract:BACKGROUND:Recently, a genome-wide association study identified a strong association between the fat mass- and obesity-associated rs9939609 single nucleotide polymorphism (SNP) and the risk of obesity in European population. However, the results in Chinese population were reported to be contradictory. Therefore, our ai...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章
doi:10.1002/dmrr.1066
更新日期:2010-02-01 00:00:00
abstract:BACKGROUND:To evaluate the association of statins and co-morbidities with new onset type 2 diabetes mellitus (T2DM) in patients 65 years and older. METHODS:This retrospective study used de-identified administrative healthcare claims and enrolment data from a Medicare Advantage Prescription Drug (MAPD) health plan offe...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章
doi:10.1002/dmrr.3310
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:Latent autoimmune diabetes in adults (LADA) exhibits significant clinical heterogeneity, but the underlying causes remain unclear. The aim of this study was to investigate whether age of onset of LADA contributes to the observed clinical heterogeneity by comparing the clinical, metabolic, and immunogenetic c...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章
doi:10.1002/dmrr.3068
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:Intranasally applied insulin is one of the antigen-specific therapies currently tested in clinical type 1 diabetes prevention trials, for example, in the Type 1 Diabetes Prediction and Prevention Study (DIPP). The possibility that the therapy may cause hypoglycaemia or local irritation and the poorly known i...
journal_title:Diabetes/metabolism research and reviews
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1002/dmrr.397
更新日期:2003-09-01 00:00:00
abstract::As basal insulin analogues are being used off-label, there is a need to evaluate their safety (maternal hypoglycaemia and fetal and perinatal outcomes) and efficacy [haemoglobin A1c(HbA1c), fasting plasma glucose, and maternal weight gain]. The aim of this review is to provide an overview of the current literature con...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章,多中心研究,随机对照试验,评审
doi:10.1002/dmrr.1213
更新日期:2011-09-01 00:00:00
abstract:BACKGROUND:Environmental factors such as nutrition or exposure to infections play a substantial role in the pathogenesis of type 1 diabetes (T1D). We have previously shown that gluten-free, non-purified diet largely prevented diabetes in non-obese diabetic (NOD) mice. In this study we tested hypothesis that early intro...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章
doi:10.1002/dmrr.748
更新日期:2008-01-01 00:00:00
abstract::The exact cause of the obesity epidemic remains unknown; however, both environmental and genetic factors are involved. People at risk of developing obesity include children with type 1 diabetes mellitus (T1DM), which in turn increases their cardiovascular disease risk. Here, we discuss the clinical and genetic factors...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章,评审
doi:10.1002/dmrr.2797
更新日期:2016-10-01 00:00:00
abstract:BACKGROUND:We conducted a meta-analysis to investigate the association of circulating tumor necrosis factor-1 (TNFR-1) and TNFR-2 with diabetic kidney disease (DKD) progression, which is the first-ever quantitative analysis of these associations thus far. Whether TNFRs were better than albumin-creatinine ratio (ACR) in...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章,meta分析
doi:10.1002/dmrr.3195
更新日期:2019-11-01 00:00:00
abstract:BACKGROUND:We evaluated whether global levels of DNA methylation status were associated with retinopathy as well as providing a predictive role of DNA methylation in developing retinopathy in a case-control study of 168 patients with type 2 diabetes. METHODS:The 5-methylcytosine content was assessed by reversed-phase ...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章
doi:10.1002/dmrr.2584
更新日期:2015-02-01 00:00:00
abstract:BACKGROUND:We conducted this research in order to evaluate the prevalence of glutamic acid decarboxylase antibodies (GADA) in Korean women with gestational diabetes mellitus (GDM), to identify the clinical characteristics of these women, and to gauge the prevalence of diabetes among them after childbirth. METHODS:We s...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章
doi:10.1002/dmrr.963
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND:High birth weight has been related to an increased risk of type 1 diabetes (T1D), while suboptimal birth weight (both high and low) has been related to obesity, insulin resistance and type 2 diabetes. Insulin resistance, as a consequence of poor metabolic control, has been described in T1D patients. The aims...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章
doi:10.1002/dmrr.2361
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:This article aims to describe the implementation and initial results of an audit-feedback quality improvement initiative in Belgian diabetic foot clinics. METHODS:Using self-developed software and questionnaires, diabetic foot clinics collected data in 2005, 2008 and 2011, covering characteristics, history ...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章
doi:10.1002/dmrr.2524
更新日期:2014-07-01 00:00:00
abstract::In Mediterranean countries, the incidence (per 100,000 per year) of Type 1 diabetes in children aged under 15 years shows wide variation from country to country, ranging from 2.45 in Macedonia to 34.4 in Sardinia. By interacting with environmental factors such as diet, toxins or viral infections, the HLA plus non-HLA ...
journal_title:Diabetes/metabolism research and reviews
pub_type: 杂志文章,评审
doi:10.1002/(sici)1520-7560(199903/04)15:2<133::aid-dm
更新日期:1999-03-01 00:00:00