MicroRNA-217 suppresses homocysteine-induced proliferation and migration of vascular smooth muscle cells via N-methyl-D-aspartic acid receptor inhibition.

Abstract:

:Hyperhomocysteine has become a critical risk for atherosclerosis and can stimulate proliferation and migration of vascular smooth muscle cells (VSMCs). N-methyl-D-aspartic acid receptor (NMDAR) is a receptor of homocysteine and mediates the effects of homocysteine on VSMCs. Bioinformatics analysis has shown NMDAR is a potential target of microRNA-217 (miR-217), which exerts multiple functions in cancer tumorigenesis and carotid plaque progression. In this study, we sought to investigate the role of miR-217 in VSMCs phenotype transition under homocysteine exposure and elucidate its effect on atherosclerotic plaque formation. After treating with several doses of homocysteine (0-8 × 10(-4)  mol/L) for 24 hours, the expression of miR-217 in HA-VSMCs and rat aortic VSMCs was not altered. Intriguingly, the expression of NMDAR mRNA and protein was reduced by homocysteine in a dose-dependent manner. Transfection of miR-217 mimic significantly inhibited the proliferation and migration of VSMCs with homocysteine treatment, while transfection of miR-217 inhibitor promoted VSMCs migration. Moreover, miR-217 mimic down-regulated while miR-217 inhibitor up-regulated NMDAR protein expression but not NMDAR mRNA expression. Through luciferase reporter assay, we showed that miR-217 could directly bind to the 3'-UTR of NMDAR. MiR-217 mimic transfection also released the inhibition of cAMP-response element-binding protein (CREB)-PGC-1α signalling induced by homocysteine. Additionally, restoration of PGC-1α expression via AdPGC-1α infection markedly suppressed VSMCs proliferation through the degradation of NADPH oxidase (NOX1) and reduction of reactive oxygen species (ROS). Collectively, our study identified the role of miR-217 in regulating VSMCs proliferation and migration, which might serve as a target for atherosclerosis therapy.

authors

Duan H,Li Y,Yan L,Yang H,Wu J,Qian P,Li B,Wang S

doi

10.1111/1440-1681.12611

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

967-75

issue

10

eissn

0305-1870

issn

1440-1681

journal_volume

43

pub_type

杂志文章
  • 2,3,4',5-Tetrahydroxystilbene-2-O-beta-D-glucoside suppresses matrix metalloproteinase expression and inflammation in atherosclerotic rats.

    abstract::1. In coronary artery disease, the typical atheromatous plaque consists of a lipid core containing various inflammatory cells and a fibrous cap composed mostly of extracellular matrix. Both matrix metalloproteinases (MMPs) and inflammation are involved in the initiation of atherosclerotic plaques and plaque instabilit...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.2007.04824.x

    authors: Zhang W,Wang CH,Li F,Zhu WZ

    更新日期:2008-03-01 00:00:00

  • Transient receptor potential (TRP) channels, vascular tone and autoregulation of cerebral blood flow.

    abstract::Members of the transient receptor potential (TRP) channel superfamily are present in vascular smooth muscle cells and play important roles in the regulation of vascular contractility. The TRPC3 and TRPC6 channels are activated by stimulation of several excitatory receptors in vascular smooth muscle cells. Activation o...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1440-1681.2007.04855.x

    authors: Brayden JE,Earley S,Nelson MT,Reading S

    更新日期:2008-09-01 00:00:00

  • Tertiapin-Q removes a mechanosensitive component of muscarinic control of the sinoatrial pacemaker in the rat.

    abstract::1. In an isolated right atrial preparation, an increase in right atrial pressure (RAP) produces an increase in atrial rate. This rate response is larger and occurs faster when there is background vagal or muscarinic stimulation. 2. We hypothesized that in the latter situation, an increase in RAP antagonizes the effect...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.2010.05408.x

    authors: Han S,Wilson SJ,Bolter CP

    更新日期:2010-09-01 00:00:00

  • Wagner-Nelson method for analysing the atypical double-peaked [13CO2] excretion curve in the [13C]-octanoate gastric emptying breath test in humans.

    abstract::1. In the [(13)C]-octanoic acid breath test, the time versus pulmonary [(13)CO(2)] excretion rate curve is analysed using mathematical curve-fitting techniques to calculate gastric emptying parameters. Thus, the goodness-of-fit highly influences the accuracy of the breath test. However, a double-peaked [(13)CO(2)] exc...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 临床试验,杂志文章

    doi:10.1111/j.1440-1681.2005.04235.x

    authors: Sanaka M,Yamamoto T,Tsutsumi H,Abe K,Kuyama Y

    更新日期:2005-07-01 00:00:00

  • Tocolytic effect of parecoxib, a new parenteral cyclo-oxygenase-2-specific inhibitor, on the spontaneous and prostaglandin-induced contractions of rat isolated myometrium.

    abstract::1. The present study was undertaken to elucidate the effects of parecoxib, a novel cyclo-oxygenase (COX)-2 inhibitor, on spontaneous and prostaglandin-induced contractions of uterine smooth muscle. 2. Non-pregnant adult Wistar rats were decapitated and dissected to isolate myometrial strips. The tissue was mounted in ...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.2007.04632.x

    authors: Ayar A

    更新日期:2007-08-01 00:00:00

  • Effects of optic tract stimulation on baroreflex vagal bradycardia in rats.

    abstract::1. Arterial baroreflexes are suppressed in stressful conditions. Intense visual stimuli can cause a threatening sensation and produce defensive reactions. 2. The present study was designed to determine whether and how electrical stimulation of the optic tract (OT) affects arterial baroreflexes, especially the heart ra...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1046/j.1440-1681.2001.03509.x

    authors: Cheng ZB,Kobayashi M,Nosaka S

    更新日期:2001-09-01 00:00:00

  • Investigation of class I anti-arrhythmic drug actions on guinea-pig cardiac mitochondrial lactate dehydrogenase activity.

    abstract::1. The effects of the Class I anti-arrhythmic drugs quinidine, procainamide, lidocaine, phenytoin and tocainide on mitochondrial lactate dehydrogenase activity were compared in guinea-pig heart preparations. 2. All the tested drugs inhibited the enzyme activity in a concentration-dependent fashion, exhibiting varying ...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1993.tb01671.x

    authors: Dzimiri N,Almotrefi AA

    更新日期:1993-04-01 00:00:00

  • Ketamine inhibits aerobic glycolysis in colorectal cancer cells by blocking the NMDA receptor-CaMK II-c-Myc pathway.

    abstract::Aerobic glycolysis plays a crucial role in cancer progression. Ketamine is often used for cancer pain relief in clinical settings. Moreover, ketamine inhibits proliferation and induces apoptosis in many cancer cell types. However, the anti-tumour mechanism of ketamine is still poorly understood. In the present study, ...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/1440-1681.13248

    authors: Hu J,Duan W,Liu Y

    更新日期:2020-05-01 00:00:00

  • Changes in renal renin gene expression in fetal sheep.

    abstract::1. Renin gene expression was investigated in kidneys from 13 foetal and four adult sheep. 2. The levels of renal renin mRNA in foetuses were greater than those in adult sheep (P < 0.001). 3. There was no significant difference in renin mRNA levels between foetuses aged 91 and 134 days (term 150 days). 4. The levels of...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1996.tb01758.x

    authors: Zhang DY,Lumbers ER,Simonetta G

    更新日期:1996-08-01 00:00:00

  • Effect of acute and subacute treatment of clonidine on blood pH, PCO2 and PO2 in mice.

    abstract::1. Measurements were made on blood pH, PCO2 and PO2 in mice after a single injection and following five weeks continuous clonidine hydrochloride treatment. 2. A single injection of clonidine hydrochloride exerts no effect on blood pH, PCO2 and PO2 but five weeks of continuous clonidine hydrochloride treatment lowers t...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1982.tb00840.x

    authors: Gan EK,Abdul Sattar MZ

    更新日期:1982-11-01 00:00:00

  • Characterization of an extracellular epitope antibody to the neuronal K-Cl cotransporter, KCC2.

    abstract::1. Ion gradients across the cell membrane are important for proper cellular communication and homeostasis. With the exception of erythrocytes, chloride (Cl), one of the most important free anions in animal cells, is not distributed at thermodynamic equilibrium across the plasma membrane. The K-Cl cotransporter (COT), ...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.2007.04621.x

    authors: Gagnon KB,Fyffe RE,Adragna NC,Lauf PK

    更新日期:2007-07-01 00:00:00

  • Stimulation of phosphatidylinositol turnover in adult rat left atria does not involve release of inositol(1,4,5)trisphosphate.

    abstract::1. The turnover rate of inositol(1,4,5)trisphosphate (Ins(1,4,5)P3) in noradrenaline-stimulated adult rat left atria was calculated from changes in specific activity and was found to equal 110 ct/min per mg tissue. In contrast, the isomers of inositol mono- and bisphosphates accumulated at a rate of 508 ct/min per mg....

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1993.tb01698.x

    authors: Anderson KE,Myers D,Woodcock EA

    更新日期:1993-05-01 00:00:00

  • Decreased oxidative stress in prehepatic portal hypertensive rat livers following the induction of diabetes.

    abstract::1. Oxidative stress (OS) is a biological entity indicated as being responsible for several pathologies, including diabetes. Diabetes can also be associated with human cirrhosis. Portal hypertension (PH), a major syndrome in cirrhosis, produces hyperdynamic splanchnic circulation and hyperaemia. The present study was d...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.2004.03963.x

    authors: Evelson P,Llesuy S,Filinger E,Rodriguez RR,Lemberg A,Scorticati C,Susemihl M,Villareal I,Polo JM,Peredo H,Perazzo JC

    更新日期:2004-03-01 00:00:00

  • The A1166C mutation in the angiotensin II type I receptor and hypertension in the elderly.

    abstract::1. Using a nested case-control study of 661 non-institutionalized elderly (> or = 60 years) residents of Dubbo, New South Wales, Australia, the aim of this study is to determine whether the A1166C polymorphism of the angiotensin II type I (AT1) receptor gene is associated with hypertension in the elderly. 2. Individua...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1046/j.1440-1681.1999.03066.x

    authors: Liyou N,Davis D,James K,Simons L,Friedlander Y,Simons J,McCallum J,Johnson A

    更新日期:1999-07-01 00:00:00

  • Recent developments concerning diet and hypertension.

    abstract::1. Recent data from randomized controlled dietary trials have shown blood pressure-lowering effects of foodstuffs and dietary patterns to be of practical importance for both individual and population blood pressure control. 2. The salient studies include Dietary Approaches to Stop Hypertension (DASH) trials, on comple...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1046/j.1440-1681.2001.03562.x

    authors: Beilin LJ,Burke V,Puddey IB,Mori TA,Hodgson JM

    更新日期:2001-12-01 00:00:00

  • Mechanisms mediating propofol protection of pulmonary epithelial cells against lipopolysaccharide-induced cell death.

    abstract::Propofol (2,6-diisopropylphenol) is an anaesthetic agent with anti-oxidant properties. The aim of the present study was to determine whether propofol can protect pulmonary epithelial (A549) cells against lipopolysaccharide (LPS)-induced cell death and, if so, the mechanisms involved. The effects of LPS alone and in co...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.2012.05694.x

    authors: Gu X,Lu Y,Chen J,He H,Li P,Yang T,Li L,Liu G,Chen Y,Zhang L

    更新日期:2012-05-01 00:00:00

  • Newcastle disease virus: a promising agent for tumour immunotherapy.

    abstract::Malignant tumours are a major cause of mortality in humans. Currently used therapeutic regimens have not improved survival rates of patients suffering from malignant tumours much because of their limited efficacy and side-effects. A therapeutic approach that uses Newcastle disease virus (NDV) represents an attractive ...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1440-1681.2011.05662.x

    authors: Zhao L,Liu H

    更新日期:2012-08-01 00:00:00

  • Studies of evolutionary temperature adaptation: muscle function and locomotor performance in Antarctic fish.

    abstract::1. Studies of evolutionary temperature adaptation of muscle and locomotor performance in fish are reviewed with a focus on the Antarctic fauna living at subzero temperatures. 2. Only limited data are available to compare the sustained and burst swimming kinematics and performance of Antarctic, temperate and tropical s...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1440-1681.1998.tb02291.x

    authors: Franklin CE

    更新日期:1998-09-01 00:00:00

  • Possible participation of NMDA and glycine receptors but not GABAA receptors in enflurane-induced opisthotonus in mice.

    abstract::1. We previously reported that volatile anaesthetics produce incidences of a transient opisthotonus in mice, a sign of CNS stimulation. This study was performed to investigate mechanisms by which enflurane-induced opisthotonus (EIO) occurs. 2. The effects of pretreatment of N-methyl-D-aspartate (NMDA) antagonists dizo...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1994.tb02546.x

    authors: Komatsu H,Nogaya J,Ueki M,Yokono S,Ogli K

    更新日期:1994-06-01 00:00:00

  • MicroRNA-645 promotes metastasis of osteosarcoma via targeting tumor suppressor NM23 nucleoside diphosphate kinase 2.

    abstract::Osteosarcoma is the most common non-hematological primary bony malignancy in children and young adults with tumor metastasis being a common event at diagnosis. Understanding the pathogenesis of metastatic osteosarcoma may help identify potential therapeutic targets. In this study, we found that the level of microRNA-6...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/1440-1681.13006

    authors: Jiao GJ,Zhang SJ,Li Y,Wu WL,Liu HC

    更新日期:2018-12-01 00:00:00

  • Blood pressure regulation via the epithelial sodium channel: from gene to kidney and beyond.

    abstract::The epithelial sodium channel (ENaC) has long been recognized as playing a vital role in blood pressure (BP) regulation due to its involvement in fluid balance. The genes encoding the three ENaC subunits are likewise important contributors to hypertension, both in rare monogenic diseases and in the general population....

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章,评审

    doi:10.1111/1440-1681.12124

    authors: Büsst CJ

    更新日期:2013-08-01 00:00:00

  • Neuropharmacology of nicotine: effects on the autoregulation of acetylcholine release by substance P and methionine enkephalin in rodent cerebral slices and toxicological implications.

    abstract::1. The neuronal release of acetylcholine (ACh) and its autoregulation by neuromodulators, substance P (SP) and methionine enkephalin (MEK), have been studied using superfused rodent cerebral slices. Nicotine exerts significant effects on autoregulation of ACh release, which may have toxicological implications. 2. Posi...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1440-1681.1995.tb01997.x

    authors: Sastry BV

    更新日期:1995-04-01 00:00:00

  • Recombinant human hepatocyte growth factor (HGF), but not rat HGF, elicits glomerular injury and albuminuria in normal rats via an immune complex-dependent mechanism.

    abstract::1. Hepatocyte growth factor (HGF) has the therapeutic potential to improve renal fibrosis and proteinuria in rodents with chronic kidney disease. In contrast, long-term administration of human HGF to normal rats reportedly elicits proteinuria. Thus, the role of HGF during proteinuria remains contentious. The aim of th...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.2011.05483.x

    authors: Mizuno S,Ikebuchi F,Fukuta K,Kato T,Matsumoto K,Adachi K,Abe T,Nakamura T

    更新日期:2011-03-01 00:00:00

  • Beta-adrenoceptor-mediated inhibition of mediator release from human peripheral blood-derived mast cells.

    abstract::1. Mast cells cultured from human peripheral blood have been used as a cell model for functional studies of human mast cells, particularly human lung mast cells. However, the beta-adrenoceptor subtype expressed by these cultured cells has not been identified. The aim of the present study was to characterize pharmacolo...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.2006.04435.x

    authors: Wang XS,Lau HY

    更新日期:2006-08-01 00:00:00

  • What makes a CGRP2 receptor?

    abstract::1. Heterogeneity in the receptors for the neuropeptide calcitonin gene-related peptide (CGRP) has been apparent for nearly 20 years. This is most clearly manifested in the observation of CGRP(8-37)-sensitive and -insensitive populations of CGRP-activated receptors. The pA(2) values for CGRP(8-37) in excess of 7 are wi...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1440-1681.2007.04703.x

    authors: Hay DL

    更新日期:2007-10-01 00:00:00

  • Dual activation of cardiac sympathetic and parasympathetic components during conditioned fear to context in the rat.

    abstract::1. The present study investigates the contribution of the sympathetic and vagal parasympathetic systems to the tachycardic response of long-lasting (40 min) conditioned fear responses to context. 2. The conditioned fear response evoked by re-exposure to a footshock chamber was tested 10 min after intravenous injection...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.2006.04519.x

    authors: Carrive P

    更新日期:2006-12-01 00:00:00

  • The CGP7930 analogue 2,6-di-tert-butyl-4-(3-hydroxy-2-spiropentylpropyl)-phenol (BSPP) potentiates baclofen action at GABA(B) autoreceptors.

    abstract::The pharmacological actions of 2,6-di-tert-butyl-4-(3-hydroxy-2-spiropentylpropyl)-phenol (BSPP), a putative presynaptic GABA(B) receptor modulator, were examined in electrically stimulated rat neocortical brain slices preloaded with [3H]-GABA or [3H]-glutamic acid. At 10 mmol/L, BSPP inhibited the release of [3H]-GAB...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.2008.04948.x

    authors: Parker DA,Marino V,Ong J,Puspawati NM,Prager RH

    更新日期:2008-09-01 00:00:00

  • Autonomic control of bronchial circulation in awake sheep during rest and behaviour.

    abstract::1. We tested the hypothesis that the pattern and the intensity of autonomic mechanisms causing vasoconstriction in the resting bronchial circulation of awake dogs also exists in awake sheep. It was also postulated that sighing behaviour and the associated bronchovascular dilatation induced by non-adrenergic, non-choli...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1997.tb02723.x

    authors: McIlveen S,White S,Parsons G

    更新日期:1997-12-01 00:00:00

  • Pressure-flow relationships in the peripheral circulation of the dog with isoprenaline.

    abstract::1. The effects of isoprenaline on the pressure-flow relationships in the peripheral circulation were studied in five anaesthetized dogs during right heart bypass procedures. 2. Isoprenaline (1 mug/kg per min) produced a significant drop in arterial pressure and arterial resistance. 3. There was a significant decrease ...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1975.tb01833.x

    authors: Green JF

    更新日期:1975-03-01 00:00:00

  • Effect of enalapril on body sodium and handling of a sodium chloride load in hypertensive and normotensive rats.

    abstract::1. The effect of enalapril on handling of an Na load and on body Na during 96 h of zero Na intake was measured in hypertensive rats (GH and SHR) and their normotensive controls (N and WKY) by a whole body counting method. 2. Enalapril treatment led to a greater fall in body Na in the first 24 h after the Na load (as e...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1992.tb00475.x

    authors: Ledingham JM,Simpson FO

    更新日期:1992-05-01 00:00:00