Characterization of single heart cell contractility by rapid imaging.


:1. A rapid imaging technique with temporal resolution of about 1 ms was employed to describe cell-length changes during the isotonic contraction cycle of adult rat ventricular myocytes at 22 degrees C. 2. Parameters of cell contraction and relaxation were defined and values obtained under control conditions and after treatment with 5 mumol/L verapamil. 3. Over 15 min, verapamil dramatically reduced the maximum shortening attained. This was associated with delay in both excitation-contraction coupling latency and the time at which maximum rate of shortening occurred. However, peak shortening was recorded earlier in the contraction cycle. Total cell-cycle time was abbreviated under the influence of verapamil and maximum rates of shortening and lengthening were depressed in a similar manner. 4. The results highlight the value of improved temporal precision in describing myocyte contractility and validate the use of the parameters defined in the single-cell model for the study of the mechanisms of action of cardiotonic agents.


Delbridge LM,Harris PJ,Morgan TO




Has Abstract


1989-03-01 00:00:00












  • Amelioration of cardiac function in chronic myocardial infarcted rats following administration of vector pcDNA3.1AM.

    abstract::1. The present study was designed to examine the cardiovascular effects of intravenously administered pcDNA3.1AM, a recombinant non-virus vector carrying a rat adrenomedullin (AM) gene translation fragment, in rats with chronic cardiac dysfunction induced by ligation of the left descending coronary artery. 2. Haemodyn...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Wang XF,Shao Y,Chen SW,Tian DZ,Huang GY,Huang Y,Yao T,Lu LM

    更新日期:2007-09-01 00:00:00

  • Effect of acute administration of prazosin on blood pressure, heart rate and plasma renin level in the conscious normotensive rat.

    abstract::This study investigated whether the specific alpha-antagonist, prazosin, stimulated basal plasma renin levels and heart rate. Furthermore the beta-adrenergic nervous system was also investigated to ascertain whether it was involved in this effect. Prazosin (0.1 or 1 mg/kg) was injected subcutaneously (s.c.) to conscio...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Atkinson J,Luthi P,Sonnay M,Boillat N

    更新日期:1986-07-01 00:00:00

  • N-methyl-D-aspartate receptors in the spinal cord mediate pressor responses to stimulation of the rostral ventrolateral medulla in the rat.

    abstract::1. Activation of bulbospinal neurons projecting from the C1 area of the rostral ventrolateral medulla evokes a pressor response. The nature of the neurotransmitters involved in mediating this response at spinal cord level has not been established. 2. Amino acid antagonists were introduced into the spinal subarachnoid ...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Mills EH,Minson JB,Pilowsky PM,Chalmers JP

    更新日期:1988-02-01 00:00:00

  • Feasibility of conducting cardiovascular outcome research in Australian general practice: results from the ANBP2 pilot study. Australian National Blood Pressure Study.

    abstract::1. The present study aimed to determine the feasibility of conducting a 5 year cardiovascular outcome trial of the treatment of 6000 elderly hypertensive patients in Australian general practices. 2. General practitioners (GPs) were invited to participate by mail and personal follow-up. Patient records were reviewed to...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Reid CM,Nelson M,Beckinsale P,Ryan P,Wing LM,Beilin LJ,Brown MA,Jennings GL,Johnston CI,Marley J,McNeil JJ,Morgan TO,Shaw J,Steven ID,West MJ

    更新日期:1997-05-01 00:00:00

  • Effects of apolipoprotein A gene polymorphisms on lipoprotein (a) concentrations in Japanese.

    abstract::1. Elevated plasma lipoprotein (a) (Lp(a)) concentrations have been correlated with an increased risk of premature cardiovascular disease. The plasma Lp(a) concentration is quantitatively heritable and the apolipoprotein (Apo) A gene is known as a major locus-determining Lp(a) concentration. 2. The aim of the present ...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Park HY,Nabika T,Notsu Y,Kobayashi S,Masuda J

    更新日期:1999-04-01 00:00:00

  • Sensitivity changes in guinea-pig atria and ileum following subacute administration of dyflos.

    abstract::1. Subacute administration of dyflos to guinea-pigs (1.2 mg/kg, s.c., daily for 10 days) led to the development of subsensitivity to carbachol in the ileum but not in the electrically stimulated left atrium. 2. Atria from dyflos-pretreated animals became supersensitive to acetylcholine but the ileum became subsensitiv...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Mitchelson F,O'Shea RA

    更新日期:1977-11-01 00:00:00

  • Protective effects of L-carnitine on myoglobinuric acute renal failure in rats.

    abstract::1. Muscle injury (rhabdomyolysis) is one of the causes of acute renal failure (ARF). Iron, free radicals and nitric oxide (NO) play a critical role in the pathogenesis of glycerol-induced myoglobinuric ARF. L-Carnitine is an anti-oxidant and prevents the accumulation of end-products of lipid peroxidation. Therefore, t...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Aydogdu N,Atmaca G,Yalcin O,Taskiran R,Tastekin E,Kaymak K

    更新日期:2006-01-01 00:00:00

  • Hantavirus diseases pathophysiology, their diagnostic strategies and therapeutic approaches: A review.

    abstract::Hantaviruses are enveloped negative (-) single-stranded RNA viruses belongs to Hantaviridae family, hosted by small rodents and entering into the human body through inhalation, causing haemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS) also known as hantavirus cardiopulmonary syndrom...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章,评审


    authors: Munir N,Jahangeer M,Hussain S,Mahmood Z,Ashiq M,Ehsan F,Akram M,Ali Shah SM,Riaz M,Sana A

    更新日期:2020-09-07 00:00:00

  • Some aldosterone-producing adrenal tumours also secrete cortisol, but present clinically as primary aldosteronism.

    abstract::1. Two patients with angiotensin-responsive aldosterone-producing adenoma (APA) and one with adrenal cortical carcinoma demonstrated autonomous secretion of cortisol as well as of aldosterone. 2. The response of cortisol and of aldosterone to ACTH did not differentiate between the two APA which secreted cortisol and t...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Tunny TJ,Klemm SA,Gordon RD

    更新日期:1990-03-01 00:00:00

  • Effects of cicletanine on the progression of renal failure in 5/6 nephrectomized hypertensive rats.

    abstract::1. The effect of cicletanine, a novel antihypertensive agent with natriuretic activity, on blood pressure and progression of renal failure of 5/6 nephrectomized spontaneously hypertensive rats with salt loading was examined. 2. All nephrectomized rats were randomly assigned to one of four groups and their diet was cha...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Nakamoto H,Suzuki H,Ohishi A,Katsumata H,Sakaguchi H,Saruta T

    更新日期:1999-03-01 00:00:00

  • Transplantation of human umbilical mesenchymal stem cells attenuates dextran sulfate sodium-induced colitis in mice.

    abstract::Ulcerative colitis is a major form of inflammatory bowel disease and increases the risk of the development of colorectal carcinoma. The anti-inflammatory and immunomodulatory properties of mesenchymal stem cells (MSC) make them promising tools for treating immune-mediated and inflammatory diseases. However, the lack o...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Lin Y,Lin L,Wang Q,Jin Y,Zhang Y,Cao Y,Zheng C

    更新日期:2015-01-01 00:00:00

  • Effects of torasemide on renal haemodynamics and function in anaesthetized dogs.

    abstract::1. We examined the effects of torasemide (0.3 and 1 mg/kg i.v.) on renal haemodynamics and function employing renal clearance and stop-flow techniques in anaesthetized dogs and compared these with furosemide (1 and 3 mg/kg i.v.). 2. Torasemide and furosemide did not influence renal haemodynamics, in the renal clearanc...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Uchida T,Hayashi K,Suzuki Y,Matsumura Y

    更新日期:1991-07-01 00:00:00

  • Role of reactive metabolites of oxygen and nitrogen in partial liver transplantation: lessons learned from reduced-size liver ischaemia and reperfusion injury.

    abstract::1. Hepatic resection with concomitant periods of ischaemia and reperfusion (I/R) is required to perform reduced-size liver (RSL) transplantation procedures, such as living donor or split liver transplantation. Although a great deal of progress has been made using these types of surgical procedures, a significant numbe...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章,评审


    authors: Urakami H,Abe Y,Grisham MB

    更新日期:2007-09-01 00:00:00

  • The possible involvement of adrenoceptors in the intestinal effect of morphine in mice.

    abstract::The inhibitory effect of morphine on intestine was observed by following the intestinal transit of a charcoal meal. This inhibitory effect of morphine was antagonized by naloxone. In addition, the inhibitory effect of morphine was also suppressed by prior administration of yohimbine and phentolamine. However, prazosin...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Wong CL

    更新日期:1984-11-01 00:00:00

  • Influence of low and normal appendicular lean mass on central blood pressure and wave reflection responses to muscle metaboreflex activation in postmenopausal women.

    abstract::Sarcopenia, defined by reduced appendicular skeletal muscle mass (ASM)/height2 (ASMI), is associated with increased arterial stiffness (brachial-ankle pulse wave velocity, baPWV) and wave reflection (augmentation index, AIx). Blood pressure (BP) responses to metaboreflex activation (post-exercise muscle ischemia, PEMI...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 信件


    authors: Figueroa A,Alvarez-Alvarado S,Jaime SJ,Johnson SA,Campbell JC,Feresin RG,Elam ML,Navaei N,Pourafshar S,Arjmandi BH

    更新日期:2016-12-01 00:00:00

  • Multiple oscillators, dynamic synchronization and sympathetic control.

    abstract::1. Intermittent bursts of activity are a robust feature of the discharges of sympathetic nerves. There are at least two major mechanisms producing such discharges: (i) phasic inputs influencing sympathetic circuits; and (ii) oscillators embedded within sympathetic networks. The functional significance of patterned and...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章,评审


    authors: Gilbey MP

    更新日期:2001-01-01 00:00:00

  • Exercise is good for your blood pressure: effects of endurance training and resistance training.

    abstract::1. Although several epidemiological studies have not observed significant independent relationships between physical activity or fitness and blood pressure, others have concluded that blood pressure is lower in individuals who are more fit or active. However, longitudinal intervention studies are more appropriate for ...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章,meta分析


    authors: Fagard RH

    更新日期:2006-09-01 00:00:00

  • Lack of effect of bromocriptine on serum levels of calcium in the rat.

    abstract::1. The effect of bromocriptine (a prolactin suppressant drug) on the serum levels of calcium was studied in the male rat. The drug was administered subcutaneously at a dosage of 100 microgram per 100 g body weight three times per day. 2. Chronic treatment with bromocriptine had no significant effect on the serum calci...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Tan CM,Tan GJ

    更新日期:1978-09-01 00:00:00

  • Effects of losartan on the cardiovascular system, renal haemodynamics and function and lung liquid flow in fetal sheep.

    abstract::1. The angiotensin type 1 (AT1) receptor antagonist, losartan (10 mg/kg) was infused intravenously into nine chronically catheterized fetal sheep (125-132 days gestation). Losartan reduced the fetal systolic (P <0.01) and diastolic (P <0.01) pressor response to 5 microg angiotensin II (AngII) i.v. from 27.4 +/- 1.5 to...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Stevenson KM,Gibson KJ,Lumbers ER

    更新日期:1996-02-01 00:00:00

  • Mechanical stretch induces endothelial nitric oxide synthase gene expression in neonatal rat cardiomyocytes.

    abstract::1. Mechanical stretch leads to cardiac hypertrophy and may ultimately cause heart failure. However, the effect of mechanical stretch on gene induction in cardiomyocytes remains to be determined. 2. In the present study, we compared transcript profiles of mechanically stretched neonatal rat cardiomyocytes with those of...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Cheng TH,Chen JJ,Shih NL,Lin JW,Liu JC,Chen YL,Chen CH,Chen JJ

    更新日期:2009-05-01 00:00:00

  • Glycyrrhizin treatment ameliorates post-traumatic stress disorder-like behaviours and restores circadian oscillation of intracranial serotonin.

    abstract::Post-traumatic stress disorder (PTSD) has become a major disease that threatens human health. Neurotransmitters and the amygdala are found to be critical in the development and maintenance of PTSD. We aim to investigate the role of glycyrrhizin in treating PTSD. Contextual fear extinction and elevated plus maze test w...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Lai S,Shi L,Jiang Z,Lin Z

    更新日期:2020-01-01 00:00:00

  • Interactive effects of morphine and dopamine receptor agonists on spatial recognition memory in mice.

    abstract::Both opiates and dopamine play important roles in learning and memory. Although synergistic action between these two neurotransmitters has been found, their functional roles remain unclear. Here, low dose morphine (2.5 mg/kg) and low dose dopamine receptor agonists (apomorphine 0.05 mg/kg; SKF38393 0.01 mg/kg; bromocr...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Chen Y,Fu Y,An Y,Cao J,Wang J,Zhang J

    更新日期:2018-04-01 00:00:00

  • Azithromycin inhibits muscarinic 2 receptor-activated and voltage-activated Ca2+ permeant ion channels and Ca2+ sensitization, relaxing airway smooth muscle contraction.

    abstract::Azithromycin (AZM) has been used for the treatment of asthma and chronic obstructive pulmonary disease (COPD); however, the effects and underlying mechanisms of AZM remain largely unknown. The effects of AZM on airway smooth muscles (ASMs) and the underlying mechanisms were studied using isometric muscle force measure...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Wang Q,Yu MF,Zhang WJ,Liu BB,Zhao QY,Luo X,Xu H,She YS,Zang DA,Qiu JY,Shen J,Peng YB,Zhao P,Xue L,Chen W,Ma LQ,Nie X,Shen C,Chen S,Chen S,Liu Q,Dai J,Qin G,Zheng YM,Wang YX,ZhuGe R,Chen J,Liu QH

    更新日期:2019-04-01 00:00:00

  • Activation of plasma prekallikrein and inactive renin by puff adder venom.

    abstract::The activation of inactive renin during incubation of human plasma with puff adder venom at pH 7.4 was found to be a complex process. Gel filtration on Sephacryl S-200 indicated that the venom contains a major peak of caseinolytic and renin-activating activity of low molecular weight. This enzyme was a metalloproteina...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Morris BJ,Lawrence CH,Catanzaro DF

    更新日期:1980-09-01 00:00:00

  • Pharmacology of a unique adenosine binding site in rat brain using a selective ligand.

    abstract::1. In order to further characterize the adenosine binding sites that we previously purified and termed P3 purinergic receptor-like protein (P3LP), a reliable binding assay method was developed using [3H]-5'-N-ethylcarboxamidoadenosine (NECA) as a radioligand and the newly developed high-affinity selective ligand 9-(6,...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Yoshioka K,Matsuda A,Nakata H

    更新日期:2001-04-01 00:00:00

  • Vasopressin V1a receptor antagonism does not reverse adrenocorticotrophin-induced hypertension in the rat.

    abstract::1. The role of arginine vasopressin (AVP) was examined in adrenocorticotrophin (ACTH)-induced hypertension in Sprague-Dawley rats using the non-peptide AVP V1a receptor antagonist OPC 21268. 2. In an acute study, six rats were pretreated with ACTH for 11 days and direct arterial blood pressure (4 h), plasma osmolality...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Fraser TB,Turner SW,Wen C,Li M,Burrell LM,Whitworth JA

    更新日期:2000-11-01 00:00:00

  • Capillary permeability in normal and hypertensive human pregnancy.

    abstract::Capillary permeability (CP) is elevated in late normal pregnancy, when compared to postpartum values. In women with pregnancy associated hypertension (PAH), pregnancy CP levels are not different from postpartum and are less than in normal pregnancy. These changes in capillary permeability are not explained by alterati...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Hunyor SN,McEniery PT,Roberts KA,Bellamy GR,Roffe DJ,Gallery ED,Gyory AZ,Boyce ES

    更新日期:1983-05-01 00:00:00

  • Possible participation of NMDA and glycine receptors but not GABAA receptors in enflurane-induced opisthotonus in mice.

    abstract::1. We previously reported that volatile anaesthetics produce incidences of a transient opisthotonus in mice, a sign of CNS stimulation. This study was performed to investigate mechanisms by which enflurane-induced opisthotonus (EIO) occurs. 2. The effects of pretreatment of N-methyl-D-aspartate (NMDA) antagonists dizo...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Komatsu H,Nogaya J,Ueki M,Yokono S,Ogli K

    更新日期:1994-06-01 00:00:00

  • Differential expression of GAS5 in rapamycin-induced reversion of glucocorticoid resistance.

    abstract::This study evaluates the association between the long noncoding RNA GAS5 levels and the anti-proliferative effect of the glucocorticoid (GC) methylprednisolone (MP) alone and in combination with rapamycin in peripheral blood mononuclear cells (PBMCs) obtained from healthy donors. The effect of MP, rapamycin, and MP pl...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章


    authors: Lucafò M,Bravin V,Tommasini A,Martelossi S,Rabach I,Ventura A,Decorti G,De Iudicibus S

    更新日期:2016-06-01 00:00:00

  • Role of chemical and mechanical stimuli in mediating bone fracture healing.

    abstract::Bone is a remarkable living tissue that provides a framework for animal body support and motion. However, under excessive loads and deformations, bone is prone is to damage through fracture. Furthermore, once the bone is weakened by osteoporosis, bone fracture can occur even after only minimal trauma. Various techniqu...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章,评审


    authors: Zhang L,Richardson M,Mendis P

    更新日期:2012-08-01 00:00:00