Ultrastructure of acetylcholine receptor aggregates parallels mechanisms of aggregation.


BACKGROUND:Acetylcholine receptors become aggregated at the developing neuromuscular synapse shortly after contact by a motorneuron in one of the earliest manifestations of synaptic development. While a major physiological signal for receptor aggregation (agrin) is known, the mechanism(s) by which muscle cells respond to this and other stimuli have yet to be worked out in detail. The question of mechanism is addressed in the present study via a quantitative examination of ultrastructural receptor arrangement within aggregates. RESULTS:In receptor rich cell membranes resulting from stimulation by agrin or laminin, or in control membrane showing spontaneous receptor aggregation, receptors were found to be closer to neighboring receptors than would be expected at random. This indicates that aggregation proceeds heterogeneously: nanoaggregates, too small for detection in the light microscope, underlie developing microaggregates of receptors in all three cases. In contrast, the structural arrangement of receptors within nanoaggregates was found to depend on the aggregation stimulus. In laminin induced nanoaggregates receptors were found to be arranged in an unstructured manner, in contrast to the hexagonal array of about 10 nm spacing found for agrin induced nanoaggregates. Spontaneous aggregates displayed an intermediate amount of order, and this was found to be due to two distinct population of nanoaggregates. CONCLUSIONS:The observations support earlier studies indicating that mechanisms by which agrin and laminin-1 induced receptor aggregates form are distinct and, for the first time, relate mechanisms underlying spontaneous aggregate formation to aggregate structure.


BMC Neurosci


BMC neuroscience


Kunkel DD,Lee LK,Stollberg J





Has Abstract


2001-01-01 00:00:00








  • Ventral and dorsal streams processing visual motion perception (FDG-PET study).

    abstract:BACKGROUND:Earlier functional imaging studies on visually induced self-motion perception (vection) disclosed a bilateral network of activations within primary and secondary visual cortex areas which was combined with signal decreases, i.e., deactivations, in multisensory vestibular cortex areas. This finding led to the...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Becker-Bense S,Buchholz HG,zu Eulenburg P,Best C,Bartenstein P,Schreckenberger M,Dieterich M

    更新日期:2012-07-16 00:00:00

  • Neurotrophins regulate ApoER2 proteolysis through activation of the Trk signaling pathway.

    abstract:BACKGROUND:ApoER2 and the neurotrophin receptors Trk and p75(NTR) are expressed in the CNS and regulate key functional aspects of neurons, including development, survival, and neuronal function. It is known that both ApoER2 and p75(NTR) are processed by metalloproteinases, followed by regulated intramembrane proteolysi...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Larios JA,Jausoro I,Benitez ML,Bronfman FC,Marzolo MP

    更新日期:2014-09-19 00:00:00

  • Spatial encoding in spinal sensorimotor circuits differs in different wild type mice strains.

    abstract:BACKGROUND:Previous studies in the rat have shown that the spatial organisation of the receptive fields of nociceptive withdrawal reflex (NWR) system are functionally adapted through experience dependent mechanisms, termed somatosensory imprinting, during postnatal development. Here we wanted to clarify 1) if mice exhi...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Thelin J,Schouenborg J

    更新日期:2008-05-21 00:00:00

  • Does conserved domain SOD1 mutation has any role in ALS severity and therapeutic outcome?

    abstract:BACKGROUND:Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative fatal disease that can affect the neurons of brain and spinal cord. ALS genetics has identified various genes to be associated with disease pathology. Oxidative stress induced bunina and lewy bodies formation can be regulated through the ...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Pal S,Tiwari A,Sharma K,Sharma SK

    更新日期:2020-10-09 00:00:00

  • Callosal connections of dorsal versus ventral premotor areas in the macaque monkey: a multiple retrograde tracing study.

    abstract:BACKGROUND:The lateral premotor cortex plays a crucial role in visually guided limb movements. It is divided into two main regions, the dorsal (PMd) and ventral (PMv) areas, which are in turn subdivided into functionally and anatomically distinct rostral (PMd-r and PMv-r) and caudal (PMd-c and PMv-c) sub-regions. We an...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Boussaoud D,Tanné-Gariépy J,Wannier T,Rouiller EM

    更新日期:2005-11-25 00:00:00

  • 10 Hz flicker improves recognition memory in older people.

    abstract:BACKGROUND:10 Hz electroencephalographic (EEG) alpha rhythms correlate with memory performance. Alpha and memory decline in older people. We wished to test if alpha-like EEG activity contributes to memory formation. Flicker can elicit alpha-like EEG activity. We tested if alpha-frequency flicker enhances memory in olde...

    journal_title:BMC neuroscience

    pub_type: 临床试验,杂志文章


    authors: Williams J,Ramaswamy D,Oulhaj A

    更新日期:2006-03-05 00:00:00

  • Laminin enhances the growth of human neural stem cells in defined culture media.

    abstract:BACKGROUND:Human neural stem cells (hNSC) have the potential to provide novel cell-based therapies for neurodegenerative conditions such as multiple sclerosis and Parkinson's disease. In order to realise this goal, protocols need to be developed that allow for large quantities of hNSC to be cultured efficiently. As suc...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Hall PE,Lathia JD,Caldwell MA,Ffrench-Constant C

    更新日期:2008-07-23 00:00:00

  • The relationship between magnetic and electrophysiological responses to complex tactile stimuli.

    abstract:BACKGROUND:Magnetoencephalography (MEG) has become an increasingly popular technique for non-invasively characterizing neuromagnetic field changes in the brain at a high temporal resolution. To examine the reliability of the MEG signal, we compared magnetic and electrophysiological responses to complex natural stimuli ...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Zhu Z,Zumer JM,Lowenthal ME,Padberg J,Recanzone GH,Krubitzer LA,Nagarajan SS,Disbrow EA

    更新日期:2009-01-15 00:00:00

  • A resting state network in the motor control circuit of the basal ganglia.

    abstract:BACKGROUND:In the absence of overt stimuli, the brain shows correlated fluctuations in functionally related brain regions. Approximately ten largely independent resting state networks (RSNs) showing this behaviour have been documented to date. Recent studies have reported the existence of an RSN in the basal ganglia - ...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Robinson S,Basso G,Soldati N,Sailer U,Jovicich J,Bruzzone L,Kryspin-Exner I,Bauer H,Moser E

    更新日期:2009-11-23 00:00:00

  • Plasticity in neuromagnetic cortical responses suggests enhanced auditory object representation.

    abstract:BACKGROUND:Auditory perceptual learning persistently modifies neural networks in the central nervous system. Central auditory processing comprises a hierarchy of sound analysis and integration, which transforms an acoustical signal into a meaningful object for perception. Based on latencies and source locations of audi...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Ross B,Jamali S,Tremblay KL

    更新日期:2013-12-05 00:00:00

  • A pre-conditioning stress accelerates increases in mouse plasma inflammatory cytokines induced by stress.

    abstract:BACKGROUND:Major depressive disorder is a prevalent disease that is inadequately treated with currently available interventions. Stress increases susceptibility to depression in patients and rodent models. Depression is also associated with aberrant activation of inflammation, such as increases in circulating levels of...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Cheng Y,Jope RS,Beurel E

    更新日期:2015-05-07 00:00:00

  • Expression, sorting and transport studies for the orphan carrier SLC10A4 in neuronal and non-neuronal cell lines and in Xenopus laevis oocytes.

    abstract:BACKGROUND:SLC10A4 belongs to the solute carrier family SLC10 whose founding members are the Na(+)/taurocholate co-transporting polypeptide (NTCP, SLC10A1) and the apical sodium-dependent bile acid transporter (ASBT, SLC10A2). These carriers maintain the enterohepatic circulation of bile acids between the liver and the...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Schmidt S,Moncada M,Burger S,Geyer J

    更新日期:2015-06-19 00:00:00

  • Death Associated Protein Kinase (DAPK) -mediated neurodegenerative mechanisms in nematode excitotoxicity.

    abstract:BACKGROUND:Excitotoxicity (the toxic overstimulation of neurons by the excitatory transmitter Glutamate) is a central process in widespread neurodegenerative conditions such as brain ischemia and chronic neurological diseases. Many mechanisms have been suggested to mediate excitotoxicity, but their significance across ...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Del Rosario JS,Feldmann KG,Ahmed T,Amjad U,Ko B,An J,Mahmud T,Salama M,Mei S,Asemota D,Mano I

    更新日期:2015-04-23 00:00:00

  • The type III neurofilament peripherin is expressed in the tuberomammillary neurons of the mouse.

    abstract:BACKGROUND:Peripherin, a type III neuronal intermediate filament, is widely expressed in neurons of the peripheral nervous system and in selected central nervous system hindbrain areas with projections towards peripheral structures, such as cranial nerves and spinal cord neurons. Peripherin appears to play a role in ne...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Eriksson KS,Zhang S,Lin L,Larivière RC,Julien JP,Mignot E

    更新日期:2008-02-24 00:00:00

  • Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model.

    abstract:BACKGROUND:Ample evidence suggests a substantial contribution of cellular and molecular changes in the spinal cord to the induction and persistence of chronic neuropathic pain conditions. While for a long time, proteases were mainly considered as protein degrading enzymes, they are now receiving growing interest as sig...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Leichsenring A,Bäcker I,Wendt W,Andriske M,Schmitz B,Stichel CC,Lübbert H

    更新日期:2008-08-12 00:00:00

  • Amyloid-β plaque formation and reactive gliosis are required for induction of cognitive deficits in App knock-in mouse models of Alzheimer's disease.

    abstract:BACKGROUND:Knock-in (KI) mouse models of Alzheimer's disease (AD) that endogenously overproduce Aβ without non-physiological overexpression of amyloid precursor protein (APP) provide important insights into the pathogenic mechanisms of AD. Previously, we reported that AppNL-G-F mice, which harbor three familial AD muta...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Sakakibara Y,Sekiya M,Saito T,Saido TC,Iijima KM

    更新日期:2019-03-20 00:00:00

  • Immunization with neural derived peptides plus scar removal induces a permissive microenvironment, and improves locomotor recovery after chronic spinal cord injury.

    abstract:BACKGROUND:Immunization with neural derived peptides (INDP) as well as scar removal-separately-have shown to induce morphological and functional improvement after spinal cord injury (SCI). In the present study, we compared the effect of INDP alone versus INDP with scar removal on motor recovery, regeneration-associated...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Rodríguez-Barrera R,Flores-Romero A,Fernández-Presas AM,García-Vences E,Silva-García R,Konigsberg M,Blancas-Espinoza L,Buzoianu-Anguiano V,Soria-Zavala K,Suárez-Meade P,Ibarra A

    更新日期:2017-01-05 00:00:00

  • Purinergic responses of calcium-dependent signaling pathways in cultured adult human astrocytes.

    abstract:BACKGROUND:The properties of Ca2+ signaling mediated by purinergic receptors are intrinsically linked with functional activity of astrocytes. At present little is known concerning Ca2+-dependent purinergic responses in adult human astrocytes. This work has examined effects of purinergic stimulation to alter levels of i...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Hashioka S,Wang YF,Little JP,Choi HB,Klegeris A,McGeer PL,McLarnon JG

    更新日期:2014-01-22 00:00:00

  • A few strong connections: optimizing information retention in neuronal avalanches.

    abstract:BACKGROUND:How living neural networks retain information is still incompletely understood. Two prominent ideas on this topic have developed in parallel, but have remained somewhat unconnected. The first of these, the "synaptic hypothesis," holds that information can be retained in synaptic connection strengths, or weig...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Chen W,Hobbs JP,Tang A,Beggs JM

    更新日期:2010-01-06 00:00:00

  • NKCC1 downregulation induces hyperpolarizing shift of GABA responsiveness at near term fetal stages in rat cultured dorsal root ganglion neurons.

    abstract:BACKGROUND:GABAA receptor-mediated neurotransmission is greatly influenced by cation-chloride cotransporter activity during developmental stages. In embryonic neurons Na-K-2Cl (NKCC1) cotransporters mediate active chloride uptake, thus increasing the intracellular chloride concentration associated with GABA-induced dep...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Chabwine JN,Talavera K,Van Den Bosch L,Callewaert G

    更新日期:2015-07-14 00:00:00

  • The roles of aromatic residues in the glycine receptor transmembrane domain.

    abstract:BACKGROUND:Cys-loop receptors play important roles in fast neuronal signal transmission. Functional receptors are pentamers, with each subunit having an extracellular, transmembrane (TM) and intracellular domain. Each TM domain contains 4 α-helices (M1-M4) joined by loops of varying lengths. Many of the amino acid resi...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Tang B,Lummis SCR

    更新日期:2018-09-06 00:00:00

  • Colocalization of connexin 36 and corticotropin-releasing hormone in the mouse brain.

    abstract:BACKGROUND:Gap junction proteins, connexins, are expressed in most endocrine and exocrine glands in the body and are at least in some glands crucial for the hormonal secretion. To what extent connexins are expressed in neurons releasing hormones or neuropeptides from or within the central nervous system is, however, un...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Westberg L,Sawa E,Wang AY,Gunaydin LA,Ribeiro AC,Pfaff DW

    更新日期:2009-04-30 00:00:00

  • Regional age-related changes in neuronal nitric oxide synthase (nNOS), messenger RNA levels and activity in SAMP8 brain.

    abstract:BACKGROUND:Nitric oxide (NO) is a multifunctional molecule synthesized by three isozymes of the NO synthase (NOSs) acting as a messenger/modulator and/or a potential neurotoxin. In rodents, the role of NOSs in sleep processes and throughout aging is now well established. For example, sleep parameters are highly deterio...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Colas D,Gharib A,Bezin L,Morales A,Guidon G,Cespuglio R,Sarda N

    更新日期:2006-12-21 00:00:00

  • Serotonin 5-HT2A and 5-HT6 receptors in the prefrontal cortex of Alzheimer and normal aging patients.

    abstract:BACKGROUND:It has been hypothesized that alterations of the serotonergic system contribute to neuropsychiatric symptoms in Alzheimer disease (AD). Cellular expressions of the two serotonergic receptors 5-HT2A and 5-HT6 have therefore been determined by immunohistochemistry in the prefrontal cortex of patients with AD (...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Lorke DE,Lu G,Cho E,Yew DT

    更新日期:2006-04-27 00:00:00

  • Age-dependent kinetics of dentate gyrus neurogenesis in the absence of cyclin D2.

    abstract:BACKGROUND:Adult neurogenesis continuously adds new neurons to the dentate gyrus and the olfactory bulb. It involves the proliferation and subsequent differentiation of neuronal progenitors, and is thus closely linked to the cell cycle machinery. Cell cycle progression is governed by the successive expression, activati...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Ansorg A,Witte OW,Urbach A

    更新日期:2012-05-07 00:00:00

  • Differential neuronal expression of receptor interacting protein 3 in rat retina: involvement in ischemic stress response.

    abstract:BACKGROUND:Receptor-interacting protein 3 (RIP3), a member of RIP family proteins, has been shown to participate in programmed necrosis or necroptosis in cell biology studies. Evidence suggests that necroptosis may be a mode of neuronal death in the retina. RESULTS:In the present study we determined the expression of ...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Huang JF,Shang L,Zhang MQ,Wang H,Chen D,Tong JB,Huang H,Yan XX,Zeng LP,Xiong K

    更新日期:2013-02-02 00:00:00

  • Non-cell autonomous impairment of oligodendrocyte differentiation precedes CNS degeneration in the Zitter rat: implications of macrophage/microglial activation in the pathogenesis.

    abstract:BACKGROUND:The zitter (zi/zi) rat, a loss-of-function mutant of the glycosylated transmembrane protein attractin (atrn), exhibits widespread age-dependent spongiform degeneration, hypomyelination, and abnormal metabolism of reactive oxygen species (ROS) in the brain. To date, the mechanisms underlying these phenotypes ...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Sakakibara S,Nakadate K,Ookawara S,Ueda S

    更新日期:2008-04-05 00:00:00

  • Expression of ATF3 and axonal outgrowth are impaired after delayed nerve repair.

    abstract:BACKGROUND:A delay in surgical nerve repair results in impaired nerve function in humans, but mechanisms behind the weakened nerve regeneration are not known. Activating transcription factor 3 (ATF3) increases the intrinsic growth state of injured neurons early after injury, but the role of long-term changes and their ...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Saito H,Dahlin LB

    更新日期:2008-09-18 00:00:00

  • Astaxanthin protects astrocytes against trauma-induced apoptosis through inhibition of NKCC1 expression via the NF-κB signaling pathway.

    abstract:BACKGROUND:Astaxanthin (ATX) is a carotenoid pigment with pleiotropic pharmacological properties that is seen as a possible drug for treating cerebral ischemic injury and subarachnoid hemorrhage. Na+-K+-2Cl- co-transporter-1 (NKCC1), an intrinsic membrane protein expressed by many cell types, is activated by various in...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Zhang M,Cui Z,Cui H,Wang Y,Zhong C

    更新日期:2017-05-10 00:00:00

  • Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence.

    abstract:BACKGROUND:Opioid agonist drugs produce analgesia. However, long-term exposure to opioid agonists may lead to opioid dependence. The analgesic and addictive properties of opioid agonist drugs are mediated primarily via the mu-opioid receptor (MOR). Opioid agonists appear to alter neuronal morphology in key brain region...

    journal_title:BMC neuroscience

    pub_type: 杂志文章


    authors: Jin J,Kittanakom S,Wong V,Reyes BA,Van Bockstaele EJ,Stagljar I,Berrettini W,Levenson R

    更新日期:2010-03-09 00:00:00