Abstract:
BACKGROUND:In order to find novel noninvasive biomarkers with high accuracy for the screening of early-stage non-small cell lung cancer (NSCLC), we investigate the predictive power of 5 microRNAs (miR-20a, miR-145, miR-21, miR223 and miR-221) as potential biomarkers in early-stage NSCLC. METHODS:In training set, 25 early-stage NSCLC patients and 25 matched healthy controls are included to assess the miRNA expression profile between early-stage NSCLC patients and healthy controls by real-time RT-PCR. We found that five of these miRNAs (miR-20a, miR-223, miR-21, miR-221 and miR-145) levels in NSCLC patients were significantly dysregulated compared with the healthy groups and thus were selected to validation set. Therefore, a validation experiment was further performed to investigate the potential predictive power of these five miRNAs based on 126 early-stage NSCLC patients, 42 NCPD patients and 60 healthy controls. The receiver operating characteristic (ROC) curves were generated for the five miRNAs. RESULTS:ROC curve analyses suggested that these five plasma miRNAs could be promising biomarkers for NSCLC, with relatively high AUC values as follows: miR-20a, 0.89 with 95% CI of [0.85-0.93]; miR-223, 0.94 with 95% CI of [0.91-0.96]; miR-21, 0.77 with 95% CI of [0.71-0.83]; miR-155, 0.92 with 95% CI of [0.89-0.96]; miR-145, 0.77 with 95% CI of [0.71-0.83]. Stratified analyses indicated that plasma miR-20a, miR-223, miR-21 and miR-145 showed better predictive value in smokers than in non-smokers, while miR-155 might be more suitable for non-smokers. In addition, all of these five miRNAs could differentiate NSCLC from controls with a higher accuracy in advanced stage and squamous carcinoma subgroups. CONCLUSIONS:In conclusion, our study suggested that five plasma miRNAs (miR-20a, miR-145, miR-21, miR-223 and miR-221) can be used as promising biomarkers in early screening of NSCLC. Nevertheless, further validation and optimizing improvement should be performed on larger sample to confirm our results.
journal_name
Respir Resjournal_title
Respiratory researchauthors
Geng Q,Fan T,Zhang B,Wang W,Xu Y,Hu Hdoi
10.1186/s12931-014-0149-3subject
Has Abstractpub_date
2014-11-25 00:00:00pages
149eissn
1465-9921issn
1465-993Xpii
s12931-014-0149-3journal_volume
15pub_type
杂志文章abstract:OBJECTIVE:To investigate the differences in the proportions of BMPR2 mutations in familial hereditary pulmonary arterial hypertension (HPAH) and idiopathic pulmonary arterial hypertension (IPAH) between males and females and the relationship between BMPR2 mutation and PAH severity. METHODS:A computer was used to searc...
journal_title:Respiratory research
pub_type: 杂志文章,meta分析
doi:10.1186/s12931-020-1309-2
更新日期:2020-02-06 00:00:00
abstract::In many model systems, cystic fibrosis (CF) phenotype airway epithelial cells in culture respond to P. aeruginosa with greater interleukin (IL)-8 and IL-6 secretion than matched controls. In order to test whether this excess inflammatory response results from the reported increased adherence of P. aeruginosa to the CF...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-6-69
更新日期:2005-07-11 00:00:00
abstract:BACKGROUND:The receptor for advanced glycation end products (RAGE) is a multiligand signal transduction receptor that can initiate and perpetuate inflammation. Its soluble isoform (sRAGE) acts as a decoy receptor for RAGE ligands, and is thought to afford protection against inflammation. With the present study, we aime...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-12-37
更新日期:2011-03-30 00:00:00
abstract:RATIONALE:Pulmonary Alveolar Proteinosis (PAP) patients exhibit an acquired deficiency of biologically active granulocyte-macrophage colony stimulating factor (GM-CSF) attributable to GM-CSF specific autoantibodies. PAP alveolar macrophages are foamy, lipid-filled cells with impaired surfactant clearance and markedly r...
journal_title:Respiratory research
pub_type: 临床试验,杂志文章
doi:10.1186/1465-9921-13-46
更新日期:2012-06-14 00:00:00
abstract:BACKGROUND:Chronic respiratory diseases (CRDs) are leading causes of morbidity worldwide. However, the spatial and temporal trends in prevalence and incidence of CRDs have not been estimated. METHODS:Based on data from the Global Burden of Diseases, Injuries, and Risk Factors Study 2017, we analyzed the prevalence and...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-020-1291-8
更新日期:2020-02-11 00:00:00
abstract:BACKGROUND:We have recently reported that protein phosphate 2A (PP2A) inactivation resulted in increased phosphorylation of the mitogen-activated protein kinase (MAPK) c-Jun N-terminal kinase 1 (JNK1) and glucocorticoid receptors (GR) at Ser(226), thereby reducing GR nuclear translocation and causing corticosteroid ins...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-016-0349-0
更新日期:2016-03-24 00:00:00
abstract:BACKGROUND:During 2007-2010, the National Health and Nutrition Examination Survey (NHANES) conducted a spirometry component which obtained pre-bronchodilator pulmonary lung function data on a nationally representative sample of US adults aged 6-79 years and post-bronchodilator pulmonary lung function data for the subse...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-14-103
更新日期:2013-10-09 00:00:00
abstract:BACKGROUND:The mechanisms during the initial phase of oxygen toxicity leading to pulmonary tissue damage are incompletely known. Increase of tumour necrosis factor alpha (TNFalpha) represents one of the first pulmonary responses to hyperoxia. We hypothesised that, in the initial phase of hyperoxia, TNFalpha activates t...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-6-10
更新日期:2005-01-21 00:00:00
abstract:BACKGROUND:Idiopathic pulmonary fibrosis (IPF) is a chronically progressive interstitial lung disease of unknown etiology. Previously, we have demonstrated the selective upregulation of the macrophage-derived chemokine CCL22 and the thymus activation-regulated chemokine CCL17 among chemokines, in a rat model of radiati...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-10-80
更新日期:2009-08-29 00:00:00
abstract:BACKGROUND:Osteoporosis is one of the systemic features of COPD. A correlation between the emphysema phenotype of COPD and reduced bone mineral density (BMD) is suggested by some studies, however, the mechanisms underlying this relationship are unclear. Experimental studies indicate that IL-1β, IL-6 and TNF-α may play ...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-12-157
更新日期:2011-12-16 00:00:00
abstract:BACKGROUND:The aim of the study is to examine the effect of limited and prolonged hyperoxia on neonatal rat lung. This is done by examining the morphologic changes of apoptosis, the expression of ceramide, an important mediator of apoptosis, the expression of inflammatory mediators represented by IL-1beta and the expre...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-7-100
更新日期:2006-07-26 00:00:00
abstract:BACKGROUND:An imbalance in the generation of pro-inflammatory leukotrienes, and counter-regulatory lipoxins is present in severe asthma. We measured leukotriene B4 (LTB4), and lipoxin A4 (LXA4) production by alveolar macrophages (AMs) and studied the impact of corticosteroids. METHODS:AMs obtained by fiberoptic bronch...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-11-71
更新日期:2010-06-07 00:00:00
abstract:BACKGROUND:Nintedanib is an approved therapy for idiopathic pulmonary fibrosis (IPF). Some patients treated with nintedanib experience weight loss. Exploratory data suggest that low body mass index or weight loss are associated with worse outcomes in patients with IPF. We investigated whether BMI at baseline or weight ...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-020-01528-4
更新日期:2020-11-25 00:00:00
abstract:BACKGROUND:The sources and measurement of reactive oxygen species (ROS) in intact organs are largely unresolved. This may be related to methodological problems associated with the techniques currently employed for ROS detection. Electron spin resonance (ESR) with spin trapping is a specific method for ROS detection, an...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-6-86
更新日期:2005-07-31 00:00:00
abstract:BACKGROUND:Idiopathic pulmonary arterial hypertension (IPAH) continues to be one of the most serious intractable diseases that might start with activation of several triggers representing the genetic susceptibility of a patient. To elucidate what essentially contributes to the onset and progression of IPAH, we investig...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-13-103
更新日期:2012-11-17 00:00:00
abstract:BACKGROUND:ABCA3 transporter (ATP-binding cassette transporter of the A subfamily) is localized to the limiting membrane of lamellar bodies, organelles for assembly and storage of pulmonary surfactant in alveolar epithelial type II cells (AECII). It transports surfactant phospholipids into lamellar bodies and absence o...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-12-4
更新日期:2011-01-07 00:00:00
abstract:BACKGROUND:Adherence to tuberculosis (TB) treatment is troublesome, due to long therapy duration, quick therapeutic response which allows the patient to disregard about the rest of their treatment and the lack of motivation on behalf of the patient for improved. The objective of this study was to develop and validate a...
journal_title:Respiratory research
pub_type: 杂志文章,多中心研究
doi:10.1186/1465-9921-13-75
更新日期:2012-09-02 00:00:00
abstract:BACKGROUND:The coexistence of COPD and asthma is widely recognized but has not been well described. This study characterizes clinical features, spirometry, and chest CT scans of smoking subjects with both COPD and asthma. METHODS:We performed a cross-sectional study comparing subjects with COPD and asthma to subjects ...
journal_title:Respiratory research
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1186/1465-9921-12-127
更新日期:2011-09-27 00:00:00
abstract:BACKGROUND:Lung inflammation in COPD is poorly controlled by inhaled corticosteroids (ICS). Strategies to improve ICS efficacy or the search of biomarkers who may select those patients candidates to receive ICS in COPD are needed. Recent data indicate that MUC1 cytoplasmic tail (CT) membrane mucin can mediate corticost...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-018-0927-4
更新日期:2018-11-20 00:00:00
abstract:BACKGROUND:To assess the symptomatic and cost burden among patients initiating long-acting bronchodilator (LABD) therapy and impact of adherence on healthcare resource use and costs. METHODS:This retrospective cohort study identified patients with COPD who were newly prescribed a LABD (long-acting muscarinic antagonis...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-015-0295-2
更新日期:2015-11-16 00:00:00
abstract:BACKGROUND:There is considerable heterogeneity in the rate of lung function decline in chronic obstructive pulmonary disease (COPD), the determinants of which are largely unknown. Observational studies in COPD indicate that low body mass index (BMI) is associated with worse outcomes, and overweight/obesity has a protec...
journal_title:Respiratory research
pub_type: 杂志文章,meta分析
doi:10.1186/s12931-019-1209-5
更新日期:2019-10-29 00:00:00
abstract:BACKGROUND:Macrolides reduce exacerbations in patients with COPD. Their effects on health status has not been assessed as primary outcome and is less clear. This study assessed the effects of prophylactic azithromycin on cough-specific health status in COPD-patients with chronic productive cough. METHODS:In this rando...
journal_title:Respiratory research
pub_type: 杂志文章,随机对照试验
doi:10.1186/1465-9921-14-125
更新日期:2013-11-14 00:00:00
abstract::Tuberculosis remains one of the world's greatest public health challenges: 2 billion persons have latent infection, 8 million people develop active tuberculosis annually, and 2-3 million die. Recently, significant advances in our understanding of the human immune response against tuberculosis have occurred. The presen...
journal_title:Respiratory research
pub_type: 杂志文章,评审
doi:10.1186/rr53
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:While adverse effects of exposure to air pollutants on respiratory health are well studied, little is known about the effect of a reduction in air pollutants on chronic respiratory symptoms and diseases. We investigated whether different declines in air pollution levels in industrialised and rural areas in G...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-11-113
更新日期:2010-08-22 00:00:00
abstract:BACKGROUND:An increasing number of epidemiological and experimental studies have associated exposure to second-hand smoke (SHS) during pregnancy with adverse outcomes in newborns. As we have previously shown in mice, in utero exposure to SHS at critical stages of fetal development, results in altered lung responses and...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-017-0591-0
更新日期:2017-06-27 00:00:00
abstract:BACKGROUND:Steroid resistant (SR) asthma is characterized by persistent airway inflammation that fails to resolve despite treatment with high doses of corticosteroids. Furthermore, SR patient airways show increased numbers neutrophils, which are less responsive to glucocorticoid. The present study seeks to determine wh...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-016-0462-0
更新日期:2016-11-16 00:00:00
abstract:BACKGROUND:Gas exchange represents the key physiological function of the lung, and is dependent upon proper formation of the delicate alveolar structure. Malformation or destruction of the alveolar gas-exchange regions are key histopathological hallmarks of diseases such as bronchopulmonary dysplasia (BPD), chronic obs...
journal_title:Respiratory research
pub_type: 杂志文章,评审
doi:10.1186/s12931-018-0837-5
更新日期:2018-08-06 00:00:00
abstract:INTRODUCTION:Bacteria have been extensively implicated in the development of smoking related diseases, such as COPD, by either direct infection or bacteria-mediated inflammation. In response to the health risks associated with tobacco exposure, the use of electronic cigarettes (e-cigs) has increased. This study compare...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-019-1206-8
更新日期:2019-12-18 00:00:00
abstract:BACKGROUND:The acute respiratory distress syndrome (ARDS) is characterized by the acute onset of hypoxemia and bilateral lung infiltrates in response to an inciting event, and is associated with high morbidity and mortality. Patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT) are at increased ...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-019-0981-6
更新日期:2019-01-21 00:00:00
abstract:BACKGROUND:Colonization of the airways with potential pathogenic bacteria is observed in a number of chronic respiratory diseases, such as COPD or cystic fibrosis. Infections with respiratory viruses are known triggers of exacerbations of these diseases. We here investigated if pre-exposure to bacteria alters the respo...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-016-0382-z
更新日期:2016-06-03 00:00:00