Identification of nuclear factor-κB inhibitors in the folk herb Rhizoma Menispermi via bioactivity-based ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry analysis.

Abstract:

BACKGROUND:Rhizoma Menispermi (RM) is the dried root of Menispermum dauricum DC, which is traditionally used to treat swelling and pain for sore throat, enteritis and rheumatic arthralgia in the clinic, but its bioactive compounds remain unclear. METHODS:In this study, RM extract was administered orally to ICR mice followed by challenging with an intratracheal Pseudomonas aeruginosa suspension. Then mortality, histological features of lung, and inflammatory cytokines were evaluated. RM treatment significantly ameliorated Pseudomonas aeruginosa-induced acute lung inflammation and reduced levels of inflammatory mediators. To screen for potential anti-inflammatory constituents of the RM extract, a simple and rapid method based on ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF MS) coupled with a luciferase reporter assay system to detect nuclear factor-κB (NF-κB) activity was established. RESULTS:Using this system, seven potential NF-κB inhibitors were detected, including sinomenine, norsinoacutin, N-norsinoacutin-β-D-glucopyranoside, 6-O-methyl-laudanosoline-13-O-glucopyranoside, magnoflorine, laurifloline and dauricinoline. Furthermore, IL-6 and IL-8 assays confirmed the anti-inflammatory effects of these potential NF-κB inhibitors, in which norsinoacutin, 6-O-methyl-laudanosoline-13-O-glucopyranoside laurifloline, dauricinoline and N-norsinoacutin-β-D-glucopyranoside were revealed as new NF-κB inhibitors. CONCLUSION:This method of UPLC-Q/TOF coupled with the luciferase reporter assay system was initially applied to the study of RM and was demonstrated to represent a simple, rapid and practical approach to screen for anti-inflammatory compounds. This study provided useful results for further investigation on the anti-inflammatory mechanism of RM.

authors

Sun D,Zhou M,Ying X,Cheng B,Han Y,Nie Y,Hou Y,Bai G

doi

10.1186/1472-6882-14-356

subject

Has Abstract

pub_date

2014-09-25 00:00:00

pages

356

issn

1472-6882

pii

1472-6882-14-356

journal_volume

14

pub_type

杂志文章
  • Anti-inflammatory effects of Neurotoxin-Nna, a peptide separated from the venom of Naja naja atra.

    abstract:BACKGROUND:Neurotoxin-Nna (NT), an analgesic peptide separated from the venom of Naja naja atra, has reported to have an exceptional specificity to block transmission of the nerve impulse by binding to the α- subunit of the nicotinic acetylcholine receptor in the membrane. However, little information is available on th...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-13-86

    authors: Ruan Y,Yao L,Zhang B,Zhang S,Guo J

    更新日期:2013-04-15 00:00:00

  • Rural Australian community pharmacists' views on complementary and alternative medicine: a pilot study.

    abstract:BACKGROUND:Complementary and alternative medicines (CAMs) are being used increasingly across the world. In Australia, community pharmacists are a major supplier of these products but knowledge of the products and interactions with other medicines is poor. Information regarding the use of CAMs by metropolitan pharmacist...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-11-103

    authors: Bushett NJ,Dickson-Swift VA,Willis JA,Wood P

    更新日期:2011-10-28 00:00:00

  • Abalone visceral extract inhibit tumor growth and metastasis by modulating Cox-2 levels and CD8+ T cell activity.

    abstract:BACKGROUND:Abalone has long been used as a valuable food source in East Asian countries. Although the nutritional importance of abalone has been reported through in vitro and in vivo studies, there is little evidence about the potential anti-tumor effects of abalone visceral extract. The aim of the present study is to ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-10-60

    authors: Lee CG,Kwon HK,Ryu JH,Kang SJ,Im CR,Ii Kim J,Im SH

    更新日期:2010-10-20 00:00:00

  • Comprehensive assessment of phenolics and antiradical potential of Rumex hastatus D. Don. roots.

    abstract:BACKGROUND:Roots of Rumex hastatus (Polygonaceae) are traditionally used for the treatment of various ailments including liver and lung diseases. In this study, various solvent extracts of R. hastatus roots, like methanolic, n-hexane, ethyl acetate, chloroform, butanol and aqueous fractions were assessed through their ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-14-47

    authors: Sahreen S,Khan MR,Khan RA

    更新日期:2014-02-08 00:00:00

  • Efficacy and safety of acupuncture for dizziness and vertigo in emergency department: a pilot cohort study.

    abstract:BACKGROUND:Dizziness and vertigo account for roughly 4% of chief symptoms in the emergency department (ED). Pharmacological therapy is often applied for these symptoms, such as vestibular suppressants, anti-emetics and benzodiazepines. However, every medication is accompanied with unavoidable side-effects. There are se...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-015-0704-6

    authors: Chiu CW,Lee TC,Hsu PC,Chen CY,Chang SC,Chiang JY,Lo LC

    更新日期:2015-06-09 00:00:00

  • Partially purified components of Uncaria sinensis attenuate blood brain barrier disruption after ischemic brain injury in mice.

    abstract:BACKGROUND:Uncaria sinensis (US) has long been used in traditional Korean medicine to relieve various nervous-related symptoms and cardiovascular disease. We recently showed the neuroprotective and cerebrovascular protective effects of US on cerebral ischemia; however, its effects on the blood-brain barrier (BBB) are p...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-015-0678-4

    authors: Seo HB,Kang BK,Kim JH,Choi YW,Hong JW,Choi BT,Shin HK

    更新日期:2015-05-27 00:00:00

  • Inhibitory effect of Yukmijihwang-tang, a traditional herbal formula against testosterone-induced benign prostatic hyperplasia in rats.

    abstract:BACKGROUND:Yukmijihwang-tang, a traditional herbal formula, has been used for treating disorder, diabetic mellitus and neurosis in China (Liu-wei-di-huang-tang in Chinese), Japan (Lokumijio-to in Japanese) and Korea for many years. In this study, we investigated the effects of Yukmijihwang-tang water extract (YJT) on t...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-12-48

    authors: Shin IS,Lee MY,Ha HK,Seo CS,Shin HK

    更新日期:2012-04-20 00:00:00

  • All-trans retinoic acids induce differentiation and sensitize a radioresistant breast cancer cells to chemotherapy.

    abstract:BACKGROUND:Radiotherapy is of critical importance in the treatment of breast cancer. However, not all patients derive therapeutic benefit and some breast cancers are resistant to the treatment, and are thus evidenced with prospective distant metastatic spread and local recurrence. In this study, we investigated the pot...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-016-1088-y

    authors: Yan Y,Li Z,Xu X,Chen C,Wei W,Fan M,Chen X,Li JJ,Wang Y,Huang J

    更新日期:2016-03-31 00:00:00

  • Antihypertensive effects of the hydro-ethanol extract of Senecio serratuloides DC in rats.

    abstract:BACKGROUND:Senecio serratuloides DC is used in folk medicine for treating hypertension, skin disorders, internal and external sores, rashes, burns and wounds. This study aimed at investigating the antihypertensive effects of the hydroethanol extract of S. serratuloides (HESS) in N-Nitro-L-arginine methyl ester (L-NAME)...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-019-2463-2

    authors: Tata CM,Sewani-Rusike CR,Oyedeji OO,Gwebu ET,Mahlakata F,Nkeh-Chungag BN

    更新日期:2019-02-28 00:00:00

  • Anti-inflammatory effects of Sanguisorbae Radix water extract on the suppression of mast cell degranulation and STAT-1/Jak-2 activation in BMMCs and HaCaT keratinocytes.

    abstract:BACKGROUND:Sanguisorbae Radix (SR) is a well-known herbal medicine used to treat inflammatory disease and skin burns in Asia. In addition, it is used to treat many types of allergic skin diseases, including urticaria, eczema, and allergic dermatitis. SR has been reported to exhibit anti-wrinkle, anti-oxidant, and anti-...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-016-1317-4

    authors: Yang JH,Yoo JM,Cho WK,Ma JY

    更新日期:2016-09-06 00:00:00

  • Effects of JSOG-6 on protection against bone loss in ovariectomized mice through regulation of osteoblast differentiation and osteoclast formation.

    abstract:BACKGROUND:JSOG-6 is used as a traditional medicine to relieve the symptoms associated with inflammation, rheumatism, and osteoporosis in Korea. In the present study, we investigated the effects of JSOG-6 on bone loss prevention both in in vitro and in vivo as well as its underlying mechanism of action. METHODS:Protec...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-14-184

    authors: Chung HJ,Cho L,Shin JS,Lee J,Ha IH,Park HJ,Lee SK

    更新日期:2014-06-06 00:00:00

  • Evaluation of selected biological capacities of Baeckea frutescens.

    abstract:BACKGROUND:Baeckea frutescens is a natural remedy recorded to be used in curing various health conditions. In Peninsular Malaysia, B. frutescens is found on the mountain tops, quartz ridge and sandy coasts. To our knowledge, there is only limited published literature on B. frutescens. METHODS:B. frutescens leaf crude ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-015-0712-6

    authors: Navanesan S,Wahab NA,Manickam S,Sim KS

    更新日期:2015-06-17 00:00:00

  • Current challenges and future directions for naturopathic medicine in Australia: a qualitative examination of perceptions and experiences from grassroots practice.

    abstract:BACKGROUND:Naturopaths are an increasingly significant part of the healthcare sector in Australia, yet despite their significant role there has been little research on this practitioner group. Currently the naturopathic profession in Australia is undergoing a period of rapid professional growth and change. However, to ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-13-15

    authors: Wardle JL,Adams J,Lui CW,Steel AE

    更新日期:2013-01-14 00:00:00

  • Assessment of free radical scavenging and anti-proliferative activities of Tinospora cordifolia Miers (Willd).

    abstract:BACKGROUND:Tinospora cordifolia (Guduchi or Amrita) is an important drug of Ayurvedic System of Medicine and found mention in various classical texts for the treatment of diseases such as jaundice, fever, diabetes, cancer and skin disease etc. In view of its traditional claims, antioxidant and anti-proliferative activi...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-017-1953-3

    authors: Polu PR,Nayanbhirama U,Khan S,Maheswari R

    更新日期:2017-09-11 00:00:00

  • Bitter melon protects against ER stress in LS174T colonic epithelial cells.

    abstract:BACKGROUND:Bitter Melon (BM) has been used as a functional food in traditional Chinese and Indian medicine for many generations and has gained a great deal of attention due to its apparent benefits in moderating some of the pathogenic processes in a variety of inflammatory conditions. BM extract (BME) has been shown to...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-016-1522-1

    authors: Kunde DA,Chong WC,Nerurkar PV,Ahuja KD,Just J,Smith JA,Guven N,Eri RD

    更新日期:2017-01-03 00:00:00

  • Antioxidant and free radical scavenging activity of Spondias pinnata.

    abstract:BACKGROUND:Many diseases are associated with oxidative stress caused by free radicals. Current research is directed towards finding naturally-occurring antioxidants of plant origin. The aim of the present study was to evaluate the in vitro antioxidant activities of Spondias pinnata stem bark extract. METHODS:A 70% met...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-8-63

    authors: Hazra B,Biswas S,Mandal N

    更新日期:2008-12-09 00:00:00

  • The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway.

    abstract:BACKGROUND:Andrographis paniculata (Burm. f.) Nees (APN), a principal constituent of a famous traditional Chinese medicine Fukeqianjin tablet which is used for the treatment of pelvic inflammatory disease (PID), has been reported to have anti-inflammatory effect in vitro. However, whether it has pharmacological effect ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-016-1466-5

    authors: Zou W,Xiao Z,Wen X,Luo J,Chen S,Cheng Z,Xiang D,Hu J,He J

    更新日期:2016-11-25 00:00:00

  • Critical pharmacokinetic and pharmacodynamic drug-herb interactions in rats between warfarin and pomegranate peel or guava leaves extracts.

    abstract:BACKGROUND:In-depth information of potential drug-herb interactions between warfarin and herbal compounds with suspected anticoagulant blood thinning effects is needed to raise caution of concomitant administration. The current study aimed to investigate the impact of co-administration of pomegranate peel and guava lea...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-019-2436-5

    authors: Alnaqeeb M,Mansor KA,Mallah EM,Ghanim BY,Idkaidek N,Qinna NA

    更新日期:2019-01-24 00:00:00

  • Saikosaponin-d increases the radiosensitivity of smmc-7721 hepatocellular carcinoma cells by adjusting the g0/g1 and g2/m checkpoints of the cell cycle.

    abstract:BACKGROUND:Saikosaponin-d (SSd), a monomer terpenoid purified from the Chinese herbal drug Radix bupleuri, has multiple effects, including anticancer properties. However, the effect of SSd on tumors exposed to radiation is largely unknown. To investigate the radiosensitizing effect of SSd and its possible mechanism, we...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-13-263

    authors: Wang BF,Dai ZJ,Wang XJ,Bai MH,Lin S,Ma HB,Wang YL,Song LQ,Ma XL,Zan Y,Min WL,Cheng YA

    更新日期:2013-10-12 00:00:00

  • Punica granatum peel extracts: HPLC fractionation and LC MS analysis to quest compounds having activity against multidrug resistant bacteria.

    abstract:BACKGROUND:Medicinal plants are rich source of traditional herbal medicine around the globe. Most of the plant's therapeutic properties are due to the presence of secondary bioactive compounds. METHODS:The present study analyzed the High Pressure Liquid Chromatography (HPLC) fractions of Puncia granatum (peel) extract...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-017-1766-4

    authors: Khan I,Rahman H,Abd El-Salam NM,Tawab A,Hussain A,Khan TA,Khan UA,Qasim M,Adnan M,Azizullah A,Murad W,Jalal A,Muhammad N,Ullah R

    更新日期:2017-05-03 00:00:00

  • The use of herbal medicines during breastfeeding: a population-based survey in Western Australia.

    abstract:BACKGROUND:Main concerns for lactating women about medications include the safety of their breastfed infants and the potential effects of medication on quantity and quality of breast milk. While medicine treatments include conventional and complementary medicines, most studies to date have focused on evaluating the saf...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-13-317

    authors: Sim TF,Sherriff J,Hattingh HL,Parsons R,Tee LB

    更新日期:2013-11-13 00:00:00

  • Anti-inflammatory potential of ellagic acid, gallic acid and punicalagin A&B isolated from Punica granatum.

    abstract:BACKGROUND:Punica granatum (pomegranate), an edible fruit originating in the Middle East, has been used as a traditional medicine for treatment of pain and inflammatory conditions such as peptic ulcer. The numerous risks associated with nonsteroidal anti-inflammatory drugs (NSAIDs) for treatment of pain and inflammatio...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-017-1555-0

    authors: BenSaad LA,Kim KH,Quah CC,Kim WR,Shahimi M

    更新日期:2017-01-14 00:00:00

  • Herbal-drug interaction induced rhabdomyolysis in a liposarcoma patient receiving trabectedin.

    abstract:BACKGROUND:Rhabdomyolysis is an uncommon side effect of trabectedin which is used for the second line therapy of metastatic sarcoma after anthracycline and ifosfamide failure. This side effect may be due to pharmacokinetic interactions caused by shared mechanisms of metabolism involving the cytochrome P450 (CYP) system...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-13-199

    authors: Strippoli S,Lorusso V,Albano A,Guida M

    更新日期:2013-07-30 00:00:00

  • Brazilin isolated from Caesalpinia sappan L. inhibits rheumatoid arthritis activity in a type-II collagen induced arthritis mouse model.

    abstract:BACKGROUND:Caesalpinia sappan L. extracts exhibit great therapeutic potential, and have been shown to have analgesic and anti-inflammatory properties. This study aimed to understand the anti-rheumatoid activity of brazilin that was isolated from ethyl acetate extract of C. sappan L. The evaluations were conducted in mi...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-015-0648-x

    authors: Jung EG,Han KI,Hwang SG,Kwon HJ,Patnaik BB,Kim YH,Han MD

    更新日期:2015-04-22 00:00:00

  • The effectiveness of fermented turmeric powder in subjects with elevated alanine transaminase levels: a randomised controlled study.

    abstract:BACKGROUND:Previous animal studies have shown that Curcuma longa (turmeric) improves liver function. Turmeric may thus be a promising ingredient in functional foods aimed at improving liver function. The purpose of the study is to investigate the hepatoprotective effect of fermented turmeric powder (FTP) on liver funct...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/1472-6882-13-58

    authors: Kim SW,Ha KC,Choi EK,Jung SY,Kim MG,Kwon DY,Yang HJ,Kim MJ,Kang HJ,Back HI,Kim SY,Park SH,Baek HY,Kim YJ,Lee JY,Chae SW

    更新日期:2013-03-08 00:00:00

  • Medicinal and ethnoveterinary remedies of hunters in Trinidad.

    abstract:BACKGROUND:Ethnomedicines are used by hunters for themselves and their hunting dogs in Trinidad. Plants are used for snakebites, scorpion stings, for injuries and mange of dogs and to facilitate hunting success. RESULTS:Plants used include Piper hispidum, Pithecelobium unguis-cati, Bauhinia excisa, Bauhinia cumanensis...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-1-10

    authors: Lans C,Harper T,Georges K,Bridgewater E

    更新日期:2001-01-01 00:00:00

  • Traditional medicine practices among community members with diabetes mellitus in Northern Tanzania: an ethnomedical survey.

    abstract:BACKGROUND:Diabetes is a growing burden in sub-Saharan Africa where traditional medicines (TMs) remain a primary form of healthcare in many settings. In Tanzania, TMs are frequently used to treat non-communicable diseases, yet little is known about TM practices for non-communicable diseases like diabetes. METHODS:Betw...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-016-1262-2

    authors: Lunyera J,Wang D,Maro V,Karia F,Boyd D,Omolo J,Patel UD,Stanifer JW,Comprehensive Kidney Disease Assessment For Risk factors, epidemiology, Knowledge, and Attitudes (CKD AFRiKA) Study.

    更新日期:2016-08-11 00:00:00

  • Subacute toxicity and stability of Soshiho-tang, a traditional herbal formula, in Sprague-Dawley rats.

    abstract:BACKGROUND:Soshiho-tang (SST, Xiao-chai-hu-tang in Chinese and Sho-saiko-to in Japanese), an oriental herbal formula, is used for treatment of chronic liver diseases. Although many researchers have studied the pharmacological properties of SST, information about its safety and toxicity is limited. Therefore, we evaluat...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-12-266

    authors: Shin IS,Lee MY,Kim Y,Seo CS,Kim JH,Shin HK

    更新日期:2012-12-27 00:00:00

  • Garlic extracts prevent oxidative stress, hypertrophy and apoptosis in cardiomyocytes: a role for nitric oxide and hydrogen sulfide.

    abstract:BACKGROUND:In ancient times, plants were recognized for their medicinal properties. Later, the arrival of synthetic drugs pushed it to the backstage. However, from being merely used for food, plants are now been widely explored for their therapeutic value. The current study explores the potential of skin and flesh extr...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-12-140

    authors: Louis XL,Murphy R,Thandapilly SJ,Yu L,Netticadan T

    更新日期:2012-08-29 00:00:00

  • Topical application of Acheflan on rat skin injury accelerates wound healing: a histopathological, immunohistochemical and biochemical study.

    abstract:BACKGROUND:Dermal wound healing involves a cascade of complex events including angiogenesis and extracellular matrix remodeling. Several groups have focused in the study of the skin wound healing activity of natural products. The phytomedicine Acheflan®, and its main active constituent is the oil from Cordia verbenacea...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-015-0745-x

    authors: Perini JA,Angeli-Gamba T,Alessandra-Perini J,Ferreira LC,Nasciutti LE,Machado DE

    更新日期:2015-06-30 00:00:00