Abstract:
BACKGROUND:Oncolytic viruses represent a promising therapy against cancers with acquired drug resistance. However, low efficacy limits its clinical application. The objective of this study is to investigate whether pharmacologically modulating autophagy could enhance oncolytic Newcastle disease virus (NDV) strain NDV/FMW virotherapy of drug-resistant lung cancer cells. METHODS:The effect of NDV/FMW infection on autophagy machinery in A549 lung cancer cell lines resistant to cisplatin (A549/DDP) or paclitaxel (A549/PTX) was investigated by detection of GFP-microtubule-associated protein 1 light chain 3 (GFP-LC3) puncta, formation of double-membrane vesicles and conversion of the nonlipidated form of LC3 (LC3-I) to the phosphatidylethanolamine-conjugated form (LC3-II). The effects of autophagy inhibitor chloroquine (CQ) and autophagy inducer rapamycin on NDV/FMW-mediated antitumor activity were evaluated both in culture cells and in mice bearing drug-resistant lung cancer cells. RESULTS:We show that NDV/FMW triggers autophagy in A549/PTX cells via dampening the class I PI3K/Akt/mTOR/p70S6K pathway, which inhibits autophagy. On the contrary, NDV/FMW infection attenuates the autophagic process in A549/DDP cells through the activation of the negative regulatory pathway. Furthermore, combination with CQ or knockdown of ATG5 significantly enhances NDV/FMW-mediated antitumor effects on A549/DDP cells, while the oncolytic efficacy of NDV/FMW in A549/PTX cells is significantly improved by rapamycin. Interestingly, autophagy modulation does not increase virus progeny in these drug resistant cells. Importantly, CQ or rapamycin significantly potentiates NDV/FMW oncolytic activity in mice bearing A549/DDP or A549/PTX cells respectively. CONCLUSIONS:These results demonstrate that combination treatment with autophagy modulators is an effective strategy to augment the therapeutic activity of NDV/FMW against drug-resistant lung cancers.
journal_name
BMC Cancerjournal_title
BMC cancerauthors
Jiang K,Li Y,Zhu Q,Xu J,Wang Y,Deng W,Liu Q,Zhang G,Meng Sdoi
10.1186/1471-2407-14-551subject
Has Abstractpub_date
2014-07-30 00:00:00pages
551issn
1471-2407pii
1471-2407-14-551journal_volume
14pub_type
杂志文章相关文献
BMC CANCER文献大全abstract:BACKGROUND:Glioblastoma is the most common and most aggressive form of malignant glioma and is very difficult to treat. Controlling tumour cell invasion and angiogenesis is essential to improve the prognosis of glioblastoma patients. Since constitutive activation of nuclear factor-κB (NF-κB) is necessary for tumour pro...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-453
更新日期:2012-10-05 00:00:00
abstract:BACKGROUND:Febrile neutropenia (FN) is common in breast cancer patients undergoing chemotherapy. Risk factors for FN have been reported, but risk models that include genetic variability have yet to be described. This study aimed to evaluate the predictive value of patient-related, chemotherapy-related, and genetic risk...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-201
更新日期:2014-03-19 00:00:00
abstract:BACKGROUND:From 2008, Danish general practitioners could refer patients suspected of having cancer to standardised cancer patient pathways (CPPs). We aimed to compare the length of the diagnostic interval in 2010 with the length of the diagnostic interval before (2004/05) and during (2007/08) the implementation of CPPs...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1317-7
更新日期:2015-04-23 00:00:00
abstract:BACKGROUND:Cell fusion is a fast and highly efficient technique for cells to acquire new properties. The fusion of somatic cells with stem cells can reprogram somatic cells to a pluripotent state. Our research on the fusion of stem cells and cancer cells demonstrates that the fused cells can exhibit stemness and cancer...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-016-2094-7
更新日期:2016-02-04 00:00:00
abstract:BACKGROUND:In this work, we have conducted a case-control study in order to assess the effect of tobacco and three genetic polymorphisms in XPC, ERCC2 and ERCC5 genes (rs2228001, rs13181 and rs17655) in bladder cancer development in Tunisia. We have also tried to evaluate whether these variants affect the bladder tumor...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-11-101
更新日期:2011-03-22 00:00:00
abstract:BACKGROUND:Brain metastases (BM) represent one of the most frequent complications related to cancer, and their treatment continues to evolve. We have evaluated the activity, toxicity and the impact on Quality of Life (QoL) of a concomitant treatment with whole brain radiotherapy (WBRT) and Temozolomide (TMZ) in patient...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-7-18
更新日期:2007-01-25 00:00:00
abstract:BACKGROUND:Recent studies have extensively investigated the role of miRNAs in colorectal cancer (CRC), and several associations have been reported. In addition, single nucleotide polymorphisms (SNPs) in promoter regions of miRNAs have been shown to affect miRNA expression. Therefore, we aimed to analyze the effect of m...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-5641-1
更新日期:2019-05-22 00:00:00
abstract:BACKGROUND:Worldwide, acute leukemia is the most common type of childhood cancer. It is particularly common in the Hispanic populations residing in the United States, Costa Rica, and Mexico City. The objective of this study was to determine the incidence of acute leukemia in children who were diagnosed and treated in p...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-11-355
更新日期:2011-08-17 00:00:00
abstract:BACKGROUND:The primary aim of treatment of a patient who has developed metastatic disease is palliation. The objectives of the current study are to describe and quantify the clinical management of women with metastatic breast cancer from the diagnosis of metastatic disease until death and to analyze differences between...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-6-179
更新日期:2006-07-06 00:00:00
abstract:BACKGROUND:There is no consensus regarding resection of the primary tumour with few or absent symptoms in patients with synchronous unresectable metastatic colorectal cancer (CRC). A potential benefit of resection of the primary tumour is to prevent complications of the primary tumour in later stages of the disease. We...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/1471-2407-14-741
更新日期:2014-10-02 00:00:00
abstract:BACKGROUND:Breast cancer is the leading cause of cancer morbidity among Shanghai and Hong Kong women, which contributes to 20-25% of new female cancer incidents. This study aimed to describe the temporal trend of breast cancer and interpret the potential effects on the observed secular trends. METHODS:Cancer incident ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3359-5
更新日期:2017-05-23 00:00:00
abstract:BACKGROUND:Peritoneal dissemination is the most common type of recurrence in advanced gastric cancer. The main mechanism is thought to be via the exfoliation of free cancer cells (FCCs) from tumor in the gastric serosa. The frequency of recurrence thus increases once the tumor cells penetrate the serosa. However, this ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-13-57
更新日期:2013-02-04 00:00:00
abstract:BACKGROUND:The miRNA isoforms (isomiRs) have been suggested to regulate the same pathways as the canonical miRNA and play an important biological role in miRNA-mediated gene regulation. Recently, a study has demonstrated that the presence or absence of all isomiRs could efficiently discriminate amongst 32 TCGA cancer t...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-5340-y
更新日期:2019-02-07 00:00:00
abstract:BACKGROUND:Genomic initiatives such as The Cancer Genome Atlas (TCGA) contain data from -omics profiling of thousands of tumor samples, which may be used to decipher cancer signaling, and related alterations. Managing and analyzing data from large-scale projects, such as TCGA, is a demanding task. It is difficult to di...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-5965-x
更新日期:2019-08-20 00:00:00
abstract:BACKGROUND:Neuroendocrine tumors (NETs) of the esophagus are extremely rare, and few cases have been reported worldwide. Thus, a comprehensive nationwide study is needed to understand the characteristics of and treatment strategy for esophageal NETs. METHODS:We collected data on esophageal NET patients from 25 hospita...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究
doi:10.1186/1471-2407-14-569
更新日期:2014-08-07 00:00:00
abstract:BACKGROUND:Omega-3 polyunsaturated fatty acids (PUFAs) were proposed to have potential effects against inflammation and cancer. However, results from epidemiology studies remain inconsistent. We aimed to explore the associations of plasma PUFAs with EC recurrence and all-cause mortality. METHOD:Women diagnosed with en...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-07035-5
更新日期:2020-06-20 00:00:00
abstract:BACKGROUND:To investigate the association between physical activity (PA) and quality of life (QoL) among breast and colorectal cancer survivors in Korea. METHODS:A total of 224 cancer survivors (151 breast and 73 colorectal cancers) who completed treatments were recruited. We measured PA level with Godin Leisure-Time ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-06819-z
更新日期:2020-05-01 00:00:00
abstract:BACKGROUND:The incidence and survival rates for colorectal cancer in Australia are among the highest in the world. With population growth and ageing there are increasing numbers of colorectal cancer survivors in the community, yet little is known of their ongoing follow up and survivorship care experiences. This study ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-4297-6
更新日期:2018-03-27 00:00:00
abstract:BACKGROUND:Improved insight into the molecular characteristics of the different ovarian cancer subgroups is needed for developing a more individualized and optimized treatment regimen. The aim of this study was to a) identify differentially expressed miRNAs in high-grade serous ovarian carcinoma (HGSC), clear cell ovar...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-80
更新日期:2014-02-11 00:00:00
abstract:BACKGROUND:We have recently described the correlation between quantitative measures of HER2 expression or HER2 homodimers by the HERmark assay and objective response (RR), time-to progression (TTP), and overall survival (OS) in an expanded access cohort of trastuzumab-treated HER2-positive patients with metastatic brea...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-10-56
更新日期:2010-02-23 00:00:00
abstract:BACKGROUND:Li-Fraumeni (LFS) and Li-Fraumeni-like (LFL) syndromes are associated to germline TP53 mutations, and are characterized by the development of central nervous system tumors, sarcomas, adrenocortical carcinomas, and other early-onset tumors. Due to the high frequency of breast cancer in LFS/LFL families, these...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-237
更新日期:2012-06-12 00:00:00
abstract:BACKGROUND:BAG-1 is a multifunctional co-chaperone of heat shock proteins (Hsc70/Hsp70) that is expressed in most cells. It interacts with Bcl-2 and Raf indicating that it might connect protein folding with other signaling pathways. Evidence that BAG-1 expression is frequently altered in human cancers, in particular in...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-4-85
更新日期:2004-11-24 00:00:00
abstract:BACKGROUND:Cysteine-rich 61 (Cyr61), a member of the CCN protein family, possesses diverse functionality in cellular processes such as adhesion, migration, proliferation, and survival. Cyr61 can also function as an oncogene or a tumour suppressor, depending on the origin of the cancer. Only a few studies have reported ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-164
更新日期:2014-03-07 00:00:00
abstract:BACKGROUND:Recent large trials have shown the survival benefits of 10-year use of tamoxifen by reducing late recurrence compared with 5-year therapy in estrogen receptor(ER)-positive breast cancer. We tried to identify clinical factors associated with the late recurrence. METHODS:We reviewed our database of ER-positiv...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-016-2423-x
更新日期:2016-07-07 00:00:00
abstract:BACKGROUND:Although radical surgery remains the cornerstone of cure in resectable gastric cancer, survival remains poor. Current evidence-based (neo)adjuvant strategies have shown to improve outcome, including perioperative chemotherapy, postoperative chemoradiotherapy and postoperative chemotherapy. However, these reg...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/s12885-018-4770-2
更新日期:2018-09-10 00:00:00
abstract:BACKGROUND:Gastric cancer is a considerable burden for worldwide patients. And diffuse gastric cancer is the most insidious subgroup with poor survival. The phenotypic characterization of the diffuse gastric cancer cell line can be useful for gastric cancer researchers. In this article, we aimed to characterize the dif...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-06814-4
更新日期:2020-04-15 00:00:00
abstract:BACKGROUND:Amphiregulin (AREG) and Epiregulin (EREG), ligands of EGFR, are reported to be predictive biomarkers of colorectal cancer patients treated with Cetuximab, an anti-EGFR antibody. The purpose of this study is to determine the correlation of AREG and EREG expression between primary colorectal cancer and corresp...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-88
更新日期:2012-03-13 00:00:00
abstract:BACKGROUND:To evaluate the safety and efficacy of intra-arterial chemotherapy (IAC) for the primary or secondary treatment of infants diagnosed with advanced retinoblastoma before 3 months of age. METHODS:This single-center retrospective study included 39 infants (42 eyes) aged ≤3 months who were diagnosed with unilat...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-5844-5
更新日期:2019-07-15 00:00:00
abstract:BACKGROUND:We compared the outcome of patients who received non-image-guided intensity-modulated radiotherapy (IMRT) with those who received helical tomotherapy (HT), a daily image-guided radiotherapy (IGRT), after surgery for oral cavity cancer (OCC). METHODS:During the period November 2006 to December 2013, a total ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-016-2165-9
更新日期:2016-02-23 00:00:00
abstract:BACKGROUND:We sought to determine the comparative diagnostic performance of standard b-value (800-1000 s/mm2) versus low b-value (400-500 s/mm2) diffusion-weighted magnetic resonance imaging (DW-MRI) in the detection of renal cell carcinoma (RCC). METHOD:After a systematic review of the available literature, studies w...
journal_title:BMC cancer
pub_type: 杂志文章,meta分析,评审
doi:10.1186/1471-2407-14-843
更新日期:2014-11-18 00:00:00