Abstract:
OBJECTIVES:In order to ensure safety of the cell-based therapy for bone regeneration, we examined in vivo biodistribution of locally or systemically transplanted osteoblast-like cells generated from bone marrow (BM) derived mononuclear cells. METHODS:BM cells obtained from a total of 13 Sprague-Dawley (SD) green fluorescent protein transgenic (GFP-Tg) rats were culture-expanded in an osteogenic differentiation medium for three weeks. Osteoblast-like cells were then locally transplanted with collagen scaffolds to the rat model of segmental bone defect. Donor cells were also intravenously infused to the normal Sprague-Dawley (SD) rats for systemic biodistribution. The flow cytometric and histological analyses were performed for cellular tracking after transplantation. RESULTS:Locally transplanted donor cells remained within the vicinity of the transplantation site without migrating to other organs. Systemically administered large amounts of osteoblast-like cells were cleared from various organ tissues within three days of transplantation and did not show any adverse effects in the transplanted rats. CONCLUSIONS:We demonstrated a precise assessment of donor cell biodistribution that further augments prospective utility of regenerative cell therapy. Cite this article: Bone Joint Res 2014;3:76-81.
journal_name
Bone Joint Resjournal_title
Bone & joint researchauthors
Okabe YT,Kondo T,Mishima K,Hayase Y,Kato K,Mizuno M,Ishiguro N,Kitoh Hdoi
10.1302/2046-3758.33.2000257subject
Has Abstractpub_date
2014-03-20 00:00:00pages
76-81issue
3issn
2046-3758pii
3/3/76journal_volume
3pub_type
杂志文章abstract:AIMS:This study aimed to investigate the effect of solute carrier family 20 member 2 (SLC20A2) gene mutation (identified from a hereditary multiple exostoses family) on chondrocyte proliferation and differentiation. METHODS:ATDC5 chondrocytes were cultured in insulin-transferrin-selenium medium to induce differentiati...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.911.BJR-2020-0112.R1
更新日期:2020-11-01 00:00:00
abstract::Bone is one of the most highly adaptive tissues in the body, possessing the capability to alter its morphology and function in response to stimuli in its surrounding environment. The ability of bone to sense and convert external mechanical stimuli into a biochemical response, which ultimately alters the phenotype and ...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.91.BJR-2019-0043.R2
更新日期:2020-05-16 00:00:00
abstract:OBJECTIVES:Small animal models of fracture repair primarily investigate indirect fracture healing via external callus formation. We present the first described rat model of direct fracture healing. METHODS:A rat tibial osteotomy was created and fixed with compression plating similar to that used in patients. The proce...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.111.2000087
更新日期:2012-11-01 00:00:00
abstract:OBJECTIVES:To assess the accuracy of patient-specific instruments (PSIs) versus standard manual technique and the precision of computer-assisted planning and PSI-guided osteotomies in pelvic tumour resection. METHODS:CT scans were obtained from five female cadaveric pelvises. Five osteotomies were designed using Mimic...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.610.BJR-2017-0094.R1
更新日期:2017-10-01 00:00:00
abstract:OBJECTIVES:We aimed to determine the effect of surgical approach on the histology of the femoral head following resurfacing of the hip. METHODS:We performed a histological assessment of the bone under the femoral component taken from retrieval specimens of patients having revision surgery following resurfacing of the ...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.29.2000179
更新日期:2013-09-18 00:00:00
abstract:OBJECTIVES:An important measure for the diagnosis and monitoring of knee osteoarthritis is the minimum joint space width (mJSW). This requires accurate alignment of the x-ray beam with the tibial plateau, which may not be accomplished in practice. We investigate the feasibility of a new mJSW measurement method from ste...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.58.2000626
更新日期:2016-08-01 00:00:00
abstract:OBJECTIVES:Osteophytes are products of active endochondral and intramembranous ossification, and therefore could theoretically provide significant efficacy as bone grafts. In this study, we compared the bone mineralisation effectiveness of osteophytes and cancellous bone, including their effects on secretion of growth ...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.62.BJR-2016-0199.R1
更新日期:2017-02-01 00:00:00
abstract:Aims:Induction heating is a noninvasive, nonantibiotic treatment modality that can potentially be used to cause thermal damage to the bacterial biofilm on the metal implant surface. The purpose of this study was to determine the effectiveness of induction heating on killing Staphylococcus epidermidis from biofilm and t...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.94.BJR-2019-0274.R1
更新日期:2020-05-16 00:00:00
abstract:Objectives:The aim of this study was to analyze drain fluid, blood, and urine simultaneously to follow the long-term release of vancomycin from a biphasic ceramic carrier in major hip surgery. Our hypothesis was that there would be high local vancomycin concentrations during the first week with safe low systemic trough...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.82.BJR-2018-0174.R2
更新日期:2019-03-02 00:00:00
abstract:Objectives:We studied subchondral intraosseous pressure (IOP) in an animal model during loading, and with vascular occlusion. We explored bone compartmentalization by saline injection. Materials and Methods:Needles were placed in the femoral condyle and proximal tibia of five anaesthetized rabbits and connected to pre...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.78.BJR-2017-0343.R2
更新日期:2018-09-15 00:00:00
abstract:OBJECTIVES:Lengthening osteotomies of the calcaneus in children are in general grafted with bone from the iliac crest. Artificial bone grafts have been introduced, however, their structural and clinical durability has not been documented. Radiostereometric analysis (RSA) is a very accurate and precise method for measur...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.45.2000292
更新日期:2015-05-01 00:00:00
abstract:OBJECTIVES:We aimed to examine the characteristics of deep venous flow in the leg in a cast and the effects of a wearable neuromuscular stimulator (geko; FirstKind Ltd) and also to explore the participants' tolerance of the stimulator. METHODS:This is an open-label physiological study on ten healthy volunteers. Duplex...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.29.2000176
更新日期:2013-09-02 00:00:00
abstract:OBJECTIVES:Pseudotumours (abnormal peri-prosthetic soft-tissue reactions) following metal-on-metal hip resurfacing arthroplasty (MoMHRA) have been associated with elevated metal ion levels, suggesting that excessive wear may occur due to edge-loading of these MoM implants. This study aimed to quantify in vivo edge-load...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.14.2000019
更新日期:2012-04-01 00:00:00
abstract:OBJECTIVES:The radiographic union score for tibial (RUST) fractures was developed by Whelan et al to assess the healing of tibial fractures following intramedullary nailing. In the current study, the repeatability and reliability of the RUST score was evaluated in an independent centre (a) using the original descriptio...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.54.2000628
更新日期:2016-04-01 00:00:00
abstract:OBJECTIVES:To assess the structure and extracellular matrix molecule expression of osteogenic cell sheets created via culture in medium with both dexamethasone (Dex) and ascorbic acid phosphate (AscP) compared either Dex or AscP alone. METHODS:Osteogenic cell sheets were prepared by culturing rat bone marrow stromal c...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.511.BJR-2016-0013.R1
更新日期:2016-11-01 00:00:00
abstract::In vivo animal experimentation has been one of the cornerstones of biological and biomedical research, particularly in the field of clinical medicine and pharmaceuticals. The conventional in vivo model system is invariably associated with high production costs and strict ethical considerations. These limitations led t...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.512.BJR-2016-0102.R2
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVES:The number of surgical procedures performed each year to treat femoroacetabular impingement (FAI) continues to rise. Although there is evidence that surgery can improve symptoms in the short-term, there is no evidence that it slows the development of osteoarthritis (OA). We performed a feasibility study to d...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.22.2000137
更新日期:2013-02-01 00:00:00
abstract:OBJECTIVES:Evidence -based medicine (EBM) is designed to inform clinical decision-making within all medical specialties, including orthopaedic surgery. We recently published a pilot survey of the Canadian Orthopaedic Association (COA) membership and demonstrated that the adoption of EBM principles is variable among Can...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.54.2000578
更新日期:2016-04-01 00:00:00
abstract:Aims:Commonly performed unicompartmental knee arthroplasty (UKA) is not designed for the lateral compartment. Additionally, the anatomical medial and lateral tibial plateaus have asymmetrical geometries, with a slightly dished medial plateau and a convex lateral plateau. Therefore, this study aims to investigate the na...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.812.BJR-2019-0114.R1
更新日期:2020-01-08 00:00:00
abstract:OBJECTIVES:Pedicle-lengthening osteotomy is a novel surgery for lumbar spinal stenosis (LSS), which achieves substantial enlargement of the spinal canal by expansion of the bilateral pedicle osteotomy sites. Few studies have evaluated the impact of this new surgery on spinal canal volume (SCV) and neural foramen dimens...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.56.2000469
更新日期:2016-06-01 00:00:00
abstract:OBJECTIVES:The goals of this study were: 1) to determine if high-fat diet (HFD) feeding in female mice would negatively impact biomechanical and histologic consequences on the Achilles tendon and quadriceps muscle; and 2) to investigate whether exercise and branched-chain amino acid (BCAA) supplementation would affect ...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.29.2000196
更新日期:2013-09-10 00:00:00
abstract::Chondrocyte hypertrophy represents a crucial turning point during endochondral bone development. This process is tightly regulated by various factors, constituting a regulatory network that maintains normal bone development. Histone deacetylase 4 (HDAC4) is the most well-characterized member of the HDAC class IIa fami...
journal_title:Bone & joint research
pub_type: 杂志文章,评审
doi:10.1302/2046-3758.92.BJR-2019-0172.R1
更新日期:2020-05-16 00:00:00
abstract:Objectives:Different criteria for assessing the reduction quality of trochanteric fractures have been reported. The Baumgaertner reduction quality criteria (BRQC) are relatively common and the Chang reduction quality criteria (CRQC) are relatively new. The objectives of the current study were to compare the reliability...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.810.BJR-2019-0032.R1
更新日期:2019-11-02 00:00:00
abstract:OBJECTIVES:This study aimed to characterise and qualitatively grade the severity of the corrosion particles released into the hip joint following taper corrosion. METHODS:The 26 cases examined were CoC/ABG Modular (n = 13) and ASR/SROM (n = 13). Blood serum metal ion levels were collected before and after revision sur...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.59.2000507
更新日期:2016-09-01 00:00:00
abstract:BACKGROUND:Resveratrol is a polyphenolic compound commonly found in the skins of red grapes. Sirtuin 1 (SIRT1) is a human gene that is activated by resveratrol and has been shown to promote longevity and boost mitochondrial metabolism. We examined the effect of resveratrol on normal and osteoarthritic (OA) human chondr...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.33.2000226
更新日期:2014-03-10 00:00:00
abstract:Objectives:The primary stability of the cementless Oxford Unicompartmental Knee Replacement (OUKR) relies on interference fit (or press fit). Insufficient interference may cause implant loosening, whilst excessive interference could cause bone damage and fracture.The aim of this study was to identify the optimal interf...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.73.BJR-2017-0193.R1
更新日期:2018-05-05 00:00:00
abstract:Aims:To evaluate graft healing of decellularized porcine superflexor tendon (pSFT) xenograft in an ovine anterior cruciate ligament (ACL) reconstruction model using two femoral fixation devices. Also, to determine if pSFT allows functional recovery of gait as compared with the preoperative measurements. Methods:A tota...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.96.BJR-2020-0030.R2
更新日期:2020-07-23 00:00:00
abstract:OBJECTIVES:To study the measurement properties of a joint specific patient reported outcome measure, a measure of capability and a general health-related quality of life (HRQOL) tool in a large cohort of patients with a hip fracture. METHODS:Responsiveness and associations between the Oxford Hip Score (a hip specific ...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.33.2000250
更新日期:2014-03-19 00:00:00
abstract:OBJECTIVE:The objective of this study was to explore dimensionality of the Oxford Hip Score (OHS) and examine whether self-reported pain and functioning can be distinguished in the form of subscales. METHODS:This was a secondary data analysis of the UK NHS hospital episode statistics/patient-reported outcome measures ...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.311.2000313
更新日期:2014-11-01 00:00:00
abstract:AIMS:The study aimed to determine whether the microRNA miR21-5p (MiR21) mediates temporomandibular joint osteoarthritis (TMJ-OA) by targeting growth differentiation factor 5 (Gdf5). METHODS:TMJ-OA was induced in MiR21 knockout (KO) mice and wild-type (WT) mice by a unilateral anterior crossbite (UAC) procedure. Mouse ...
journal_title:Bone & joint research
pub_type: 杂志文章
doi:10.1302/2046-3758.910.BJR-2020-0140.R1
更新日期:2020-10-01 00:00:00